Browse publications by year: 2024

  1. Chong WN, Adiana G, Baharom IN, Kamaruzzaman BY, Takarina ND, Ong MC
    Mar Pollut Bull, 2024 Nov 19;209(Pt B):117281.
    PMID: 39566141 DOI: 10.1016/j.marpolbul.2024.117281
    Heavy metal pollution in aquatic ecosystems can lead to harmful accumulation in organisms like stingrays, posing potential health risks to humans. To assess this risk, 45 stingrays were sampled from Johor Waters (Pontian, Muar, and Batu Pahat) and analyzed for heavy metal content using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The average concentrations of Cu, Zn, As, Cd, Pb, and Hg in the fillets were 0.982±0.637, 31.3±5.93, 93.57±24.5, 0.039±0.047, 0.158±0.036, and 0.592±0.424 μg/g dw, respectively. Arsenic (As) was found at the highest concentration. The study indicates that fish size does not directly influence heavy metal accumulation and that metal levels vary by location. Calculated consumption limits for different metals are crucial for human safety. This data is valuable for evaluating contamination risks and guiding future research to protect marine ecosystems in Johor Waters.
  2. Gao F, Chui PL, Che CC, Wang K
    Geriatr Nurs, 2024 Nov 19;61:192-199.
    PMID: 39566231 DOI: 10.1016/j.gerinurse.2024.10.061
    OBJECTIVE: To determine the mediating role of family cohesion in the relationship between death anxiety and readiness toward advance care planning (ACP).

    METHODS: This study included 965 Chinese community-dwelling older adults. Pearson correlation coefficient was conducted to assess the relationship between readiness toward ACP, death anxiety, and family cohesion. Structural equation model was used to examine the study hypothetical model.

    RESULTS: 965 valid questionnaires were collected. Death anxiety is significantly related to the readiness toward ACP (r = -0.437, P < 0.01) and family cohesion (r = -0.444, P < 0.01), and family cohesion exhibited a positive correlation with readiness toward ACP (r = 0.499, P < 0.01). Family cohesion partially mediated the effect of death anxiety on readiness toward ACP, accounting for 35.94 % of the total effect.

    CONCLUSIONS: Family cohesion mediates the relationship between death anxiety and readiness toward ACP. Healthcare professionals should implement measures to alleviate death anxiety and promote family cohesion in older adults, thereby enhancing their readiness toward ACP.

  3. Waheed A, Akram S, Butt FW, Liaqat Z, Siddique M, Anwar F, et al.
    J Chromatogr A, 2024 Nov 08;1739:465503.
    PMID: 39566285 DOI: 10.1016/j.chroma.2024.465503
    Ionic liquids (ILs) have emerged as more desirable liquids than conventional solvents for chemistry, material science, engineering and environmental science. The scientific literature reveals an exponential increase in the number of research projects aimed at exploring the chromatographic features of ionic liquids. The review provides sound scientific data to examine the structural characteristics of ionic liquids that make them ideal for use in chromatography. This contribution is distinctive since it integrates the synthesis, benefits, drawbacks, and possible uses of ionic liquids in several chromatographic separation processes. Keeping the cation the same, the introduction of different anions is also possible, and this strategy leads to the synthesis of a series of different ionic liquids with varying properties. A detailed probe is given on the influence of ionic liquid structure and properties on their chromatographic behavior, both as stationary phase and mobile phase and/or mobile phase additives. Ionic liquid based immobilized stationary phases and their analyte retention mechanisms (hydrogen bonding, electrostatic forces of attraction, π-π stacking, ion exchange, and hydrophilic interactions, etc.) are critically discussed. Finally, a thorough analysis of the literature suggests that IL-based stationary phases may undergo multi-mode and more flexible retention mechanisms. Their dual polarity can facilitate interaction with both polar and non-polar compounds. Similarly, using IL as a mobile phase can offer more pragmatic and sustainable options for enantiomer separation.
  4. Mohamed Thaha UAB, Wan Mohamad WM, Nik Husain NR, Yusop N, Mohamud R, Wan Ghazali WS
    Int Immunopharmacol, 2024 Nov 19;144:113597.
    PMID: 39566387 DOI: 10.1016/j.intimp.2024.113597
    BACKGROUND: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by dysregulated immune responses and inflammation. Interleukin-37 (IL-37) is a recently discovered immunomodulatory cytokine with potential anti-inflammatory properties. This systematic review explores the relationship between IL and 37 and SLE disease activity, and evaluates its potential as a therapeutic agent.

    METHODS: Electronic databases were searched for studies investigating IL-37 and SLE. Data on IL-37 levels, SLE Disease Activity Index (SLEDAI) score, genetic polymorphisms, and its therapeutic effects from pre-clinical studies were extracted.

    RESULTS: Previous studies presented conflicting findings on IL-37 levels in SLE patients. Some reported positive correlations with disease activity, while others observed associations between lower IL-37 and increased activity. Genetic variations in the IL-37 gene linked to SLE susceptibility have been reported. Pre-clinical studies using engineered mesenchymal stem cells or direct IL-37 treatment showed promise in reducing disease severity in mouse models and cell cultures of SLE. The analysis of multiple studies reveals that IL-37 expression varies significantly across different SLE subtypes.

    CONCLUSIONS: While a potential link exists between IL and 37 and disease activity, genetic predisposition, and therapeutic benefit, further research is needed. Future studies with standardized designs, larger and more diverse populations, and mechanistic investigations are crucial to determine the therapeutic potential of IL-37 for SLE. This review highlights the need for well-designed clinical trials to evaluate the safety and efficacy of IL-37 therapy in patients with SLE.

  5. Lee ZM, Goh BH, Khaw KY
    Planta Med, 2024 Nov 20.
    PMID: 39566518 DOI: 10.1055/a-2446-2146
    Aloe vera is commonly used as traditional medicine for cutaneous wound healing. Nonetheless, the wound healing mechanisms of Aloe vera remain unclear. This review aims to provide insight into the molecular mechanisms of Aloe vera in promoting cutaneous wound healing, with particular emphasis on the mechanisms that stimulate cell proliferation and migration. Aloe vera has been shown to upregulate growth factors such as keratinocyte growth factor-1 (KGF-1), transforming growth factor-β (TGF-β), cyclin D1, insulin-like growth factor 1 (IGF-1), vascular endothelial growth factor (VEGF), basic fibroblastic growth factor (bFGF), and microfibril-associated glycoprotein 4 (MFAP4), as well as collagen, fibrillin, elastin, α-smooth muscle actin (α-SMA), integrins, and platelet endothelial cell adhesion molecule 1 (PECAM-1, also known as CD31), while downregulating the expression of matrix metalloproteinases (MMPs). In addition, Aloe vera was also found to upregulate PI3K/Akt and MAPK pathways, as well as the TGF-β signalling pathway via Smad proteins. Furthermore, molecular docking studies revealed that certain chemical constituents of Aloe vera bind to some of the forementioned growth factors or signalling molecules. With regards to current applications, although human clinical trials have reported positive results from using Aloe vera in healing open wounds and burns and alleviating some inflammatory skin diseases, the current commercial uses of Aloe vera remain largely focused on cosmetic products. Thus, greater advances are required to promote the use of Aloe vera products in clinical settings.
  6. Niu Q, Li D, Zhang J, Piao Z, Xu B, Xi Y, et al.
    Ageing Res Rev, 2024 Dec;102:102593.
    PMID: 39566741 DOI: 10.1016/j.arr.2024.102593
    Alzheimer's disease (AD), commonly known as senile dementia, is a neurodegenerative disease with insidious onset and gradually worsening course. The brain is particularly sensitive to senescence, and neuronal senescence is an important risk factor for the occurrence of AD. However, the exact pathogenesis between neuronal senescence and AD has not been fully elucidated so far. Neuronal senescence is characterized by the permanent stagnation of the cell cycle, and the changes in its structure, function, and microenvironment are closely related to the pathogenesis and progression of AD. In recent years, studies such as the Aβ cascade hypothesis and Tau protein phosphorylation have provided new strategies for the therapy of AD, but due to the complexity of the etiology of AD, there are still no effective treatment measures. This article aims to deeply analyze the pathogenesis between AD and neuronal senescence, and sort out various existing therapeutic methods, to provide new ideas and references for the clinical treatment of AD.
    MeSH terms: Aging/pathology; Aging/physiology; Animals; Brain/metabolism; Brain/pathology; Humans
  7. Goh SM, Dassanayake MK, Foan CC, Wiart C, Symonds R, Khoo TJ, et al.
    Microb Pathog, 2024 Nov 18.
    PMID: 39566830 DOI: 10.1016/j.micpath.2024.107134
    BACKGROUND AND OBJECTIVES: Plants contain a wide variety of bioactive compounds, which have attracted the interest of researchers in finding novel sources of natural medicine. In the following paper, we aim to evaluate the antibacterial potential of extract fractions associated with Parkia speciosa pods and beans against human pathogenic bacteria.

    METHODS: Antimicrobial activity was determined with disc diffusion and broth microdilution assays against eight skin colonising microorganisms including Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia followed by further fractionation of the pods ethyl acetate fraction by column chromatography along with preparative thin-layer chromatography. Quantification of bacterial death mechanism was elucidated by the measurement of hole size in cell wall that has been induced by extract constituents via field-emission scanning electron microscopy (FESEM).

    RESULTS: Four fractions showed significant antimicrobial activity against the six microorganisms tested (p<0.01), with inhibition zones ranging from 35.67 to 17.00 mm, and minimum inhibitory concentration ranging from 6.25 to 50.00 mg/ml in which the pods ethyl acetate fraction was the most effective. The methanol fraction isolated from the pods ethyl acetate fraction was much more effective with a four-fold increase from 6.25 to 1.25 mg/ml against S. epidermidis. The disintegration of S. aureus was due to chronic cell wall alterations with pore creation, invaginations and morphological disorganisation. Autolysis in bacterial cells via the expression of peptidoglycan-disrupting lysozyme or bacterial murein hydrolase was postulated. A significantly large pore with a mean diameter of 293.7 nm was detected in the cell wall of S. aureus.

    CONCLUSION: P. speciosa fraction could be a potential novel source for the development of a natural antibacterial agent.

  8. Saleh RO, Hamad HA, Najim MA, Menon SV, Kaur M, Sivaprasad GV, et al.
    Cell Biochem Biophys, 2024 Nov 20.
    PMID: 39567423 DOI: 10.1007/s12013-024-01617-x
    Exosomes are extracellular vesicles with a diameter ranging from 40 to 160 nm. They are produced by hepatocytes, cholangiocytes, hepatic stellate cells (HSCs), liver sinusoidal endothelial cells (LSECs) and Kupffer cells in liver tissue. The secretion of exosomes might vary in quantity and composition in reaction to multiple triggers and various stages of disease. They transport various payloads, such as proteins, DNAs, and RNAs, and enable cell interaction to regulate myriad physiological and pathological processes in liver tissue. Long non-coding RNAs (lncRNAs) are a crucial component of exosomes with an excellent capability to regulate multiple cellular activities such as differentiation, development, metabolism, proliferation, apoptosis, and activation. With the advancements in transcriptomic and genomic study methods and database management technology, the functions and mechanisms of exosomal lncRNAs in liver diseases have been well-studied. This article delves into the detailed role of exosomal lncRNAs in liver disease onset and progression, ranging from hepatocellular carcinoma (HCC) to liver fibrosis drug-induced liver damage (DILI) and steatotic liver diseases.
  9. Zhang J, Noor ZZ, Baharuddin NH, Setu SA, Hamzah MAAM, Zakaria ZA
    World J Microbiol Biotechnol, 2024 Nov 21;40(12):387.
    PMID: 39567441 DOI: 10.1007/s11274-024-04194-6
    This study highlights the biosorption capacity for Cd (II), Cu (II) and Pb (II) by a locally isolated Pseudomonas aeruginosa DR7. At initial concentrations of 150 mg L-1 and 240 min of contact time, P. aeruginosa DR7 showed a 62.56 mg/g removal capacity for Cd (II) at an optimum pH of 6.0, 72.49 mg/g for Cu (II) at an optimum pH of 6.0, and 94.2 mg/g for Pb (II) at an optimum pH of 7.0. The experimental data of Cd (II), Cu (II), and Pb (II) adsorbed by the pseudo-second-order kinetic model correlates well with P. aeruginosa DR7, with R2 all above 0.99, showing that the fitting effect was satisfactory. The isothermal adsorption processes of Cd (II) (0.980) and Cu (II) (0.986) were more consistent with the Freundlich model, whereas Pb (II) was more consistent with the Langmuir model (0.978). FTIR analysis suggested the involvement of hydroxyl, carbonyl, carboxyl, and amine groups present in the inner regions of P. aeruginosa cells during the biosorption process. SEM-EDS analysis revealed that after contact with metals, there were slight changes in the surface appearance of the cells, which confirmed the deposition of metals on the bacterial surface. There was also the possibility of the metals being translocated into the bacterial inner regions by the appearance of electron-dense particles, as observed using TEM. As a conclusion, the removal of metals from solutions using P. aeruginosa DR7 was a plausible alternative as a safe, cheap, and easily used biosorbent.
    MeSH terms: Adsorption; Biodegradation, Environmental*; Hydrogen-Ion Concentration; Kinetics; Soil Microbiology*; Spectroscopy, Fourier Transform Infrared
  10. Karthika A, Sudhakar C, Karuppasamy P, Tamilselvi B, Meena S, Anantharaju KS, et al.
    Sci Rep, 2024 Nov 20;14(1):28714.
    PMID: 39567594 DOI: 10.1038/s41598-024-80146-2
    A highly selective, sensitive caffeic acid (CA) detection based on calcium oxide nanoparticles (CaO NPs) derived from extract of Moringa oleifera leaves decorated graphitic carbon nitride covalently grafted poly vinyl alcohol (CaO/g-C3N4/PVA) nanocomposite modified glassy carbon electrode (GCE) was studied. A facile sonochemical method was adapted to synthesis nanomaterials and characterized by HR-TEM (High resolution transmission electron microscopy), FT-IR (Fourier transform infrared spectroscopy), XRD (X-ray diffraction), FE-SEM (Field emission scanning electron microscopy), EDX (Energy dispersive X-ray analysis), Mapping and BET (Brunauer-Emmett-Teller) analysis, and electrochemical techniques. The nanocomposite modified GCE exhibited an excellent catalytic performance to the oxidation of CA under optimized conditions owing to better electron transfer efficiency, conductivity and high surface area of the electrode material. The present electrochemical sensor showed high selectivity towards the determination of 10 µM CA in the presence of 100-fold higher concentrations of interferents. The modified CA sensor exhibited a wide sensing linear range from 0.01 µM to 70 µM and the detection limit (LOD) was found to be 0.0024 µM (S/N = 3) in 0.1 M phosphate buffer saline (PBS) as a supporting electrolyte at pH 7.0. The fabricated CA sensor provides an excellent stability, reproducibility and selectivity for the determination of CA. The modified CA sensor was applied to real blood plasma samples and obtained good recovery (97.6-100.1%) results.
  11. Shahrul AI, Pauzzi ER, Abas NDA, Yahya NA, Khan KAM, Nor MM
    BMC Public Health, 2024 Nov 20;24(1):3224.
    PMID: 39567923 DOI: 10.1186/s12889-024-20460-6
    BACKGROUND: The increasing number of young adults seeking cheaper and easily accessible orthodontic treatment from unlicensed practitioners in Malaysia poses significant risks to patients. Therefore, it is essential to evaluate their motivations and awareness regarding such practices. The objective of our study was to assess the knowledge, awareness, and perceptions of non-dentists offering orthodontic treatment among the Malaysian young adult population.

    METHODS: This was a cross-sectional study. An online self-administered questionnaire was distributed to Malaysian citizens aged 18-37 years. The questionnaire consisted of 11 questions that investigated their awareness of non-dentists offering orthodontic treatment, the harmful effects of braces fitted by non-dentists, and potential strategies to mitigate this phenomenon.

    RESULTS: The study was completed by 426 participants, predominantly Malay, with a mean age of 22.9 years. A total of 76.1% reported awareness of braces fixed by non-dentists, primarily through social media platforms such as Instagram and Facebook. Lower cost emerged as the predominant motive (83.6%) for opting for non-dentist orthodontic treatment, followed by no waiting list (48.8%). Notably, the majority of participants acknowledged the illegality (70%) and potential harm (77%) associated with non-dentists providing orthodontic treatment. Legal enforcement (53.1%) was identified as the preferred method for mitigating this practice. Occupation significantly influenced knowledge of illegal orthodontic treatment (p  0.05).

    CONCLUSION: The survey revealed that young adults are aware of and informed about non-dentists offering orthodontic treatment. While they identified cost as the primary reason for seeking such services, they also recognized legislation and public awareness through campaigns and social media as effective strategies to address this issue. Additionally, significant differences in legal awareness were observed among different occupational levels.

    MeSH terms: Adolescent; Adult; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice*; Malaysia; Male; Orthodontics, Corrective/psychology; Surveys and Questionnaires; Young Adult
  12. Maspero J, De Paula Motta Rubini N, Zhang J, Sanclemente G, Amador JR, El Sayed MH, et al.
    World Allergy Organ J, 2024 Nov;17(11):100966.
    PMID: 39568731 DOI: 10.1016/j.waojou.2024.100966
    [This corrects the article DOI: 10.1016/j.waojou.2022.100724.].
  13. Farwa U, Sandhu ZA, Kiran A, Raza MA, Ashraf S, Gulzarab H, et al.
    RSC Adv, 2024 Nov 19;14(50):37164-37195.
    PMID: 39569125 DOI: 10.1039/d4ra05642f
    The global problem of ecological safety and public health necessitates, the development of new sustainable ideas for pollution remediation. In recent development, metal-organic frameworks (MOF) are the emerging technology with remarkable potential, which have been employed in environmental remediation. MOFs are networks that are created by the coordination of metals or polyanions with ligands and contain organic components that can be customized. The interesting features of MOFs are a large surface area, tuneable porosity, functional diversity, and high predictability of pollutant adsorption, catalysis, and degradation. It is a solid material that occupies a unique position in the war against environmental pollutants. One of the main benefits of MOFs is that they exhibit selective adsorption of a wide range of pollutants, including heavy metals, organics, greenhouse gases, water and soil. Only particles with the right combination of pore size and chemical composition will achieve this selectivity, derived from the high level of specificity. Besides, they possess high catalytic ability for the removal of pollutants by means of different methods such as photocatalysis, Fenton-like reactions, and oxidative degradation. By generating mobile active sites within the framework of MOFs, we can not only ensure high affinity for pollutants but also effective transformation of toxic chemicals into less harmful or even inert end products. However, the long-term stability of MOFs is becoming more important as eco-friendly parts are replaced with those that can be used repeatedly, and systems based on MOFs that can remove pollutants in more than one way are fabricated. MOFs can reduce waste production, energy consumption as compared to the other removal process. With its endless capacities, MOF technology brings a solution to the environmental cleansing problem, working as a flexible problem solver from one field to another. The investigation of MOF synthesis and principles will allow researchers to fully understand the potential of MOFs in environmental problem solving, making the world a better place for all of us.
  14. Zhang B, Liu L, Meng D, Kue CS
    Acta Radiol, 2024 Nov 21.
    PMID: 39569554 DOI: 10.1177/02841851241291931
    BACKGROUND: Cervical cancer is a major cause of morbidity and mortality among gynecological malignancies. Diagnostic imaging of lymph node (LN) metastasis for prognosis and staging is used; however, the accuracy in classifying the stage needs to improve.

    PURPOSE: To examine the accuracy of AI-based radiomics in diagnosis, prognosis assessment and predicting the diagnostic value of radiomics for pelvic LN metastasis in cervical cancer patients.

    MATERIAL AND METHODS: The study included 118 female patients with 660 LNs and 118 merged LNs. Four imaging histology models-decision tree, random forest, logistic regression, and support vector machine (SVM)-were created in this study. The imaging histology features were extracted from both the independent and merged LN groups. The AUC values for the test sets and the training sets of the four imaging histology models were compared for the independent LN group and the merged LN group. The DeLong test was used to compare the models.

    RESULT: The imaging histology prediction model developed in the merged LN group outperformed the independent LN group in terms of test set AUC (0.668 vs. 0.535 for decision tree, 0.841 vs. 0.627 for logistic regression, 0.785 vs. 0.637 for random forest, 0.85 vs. 0.648 for SVM) and accuracy (0.754 vs. 0.676 for decision tree, 0.780 vs. 0.671 for random forest, 0.848 vs. 0.685 for logistic regression, 0.822 vs. 0.657 for SVM).

    CONCLUSION: The constructed SVM imaging histology model for the merged LN group might be advantageous in predicting pelvic LN metastasis in cervical cancer.

  15. Wong KYR, Hakim I, Sawali H, Lim RCA, Mohd Mohsin NK
    Medeni Med J, 2024 Jun 28;39(2):132-135.
    PMID: 38940503 DOI: 10.4274/MMJ.galenos.2024.37729
    Tumors occurring in the submandibular space are infrequent among pediatric patients, and benign peripheral nerve tumors in this region are exceptionally rare. This study describes the uncommon occurrence of a schwannoma in the submandibular space in a child and describes its management. A 7-year-old child presented with a gradually enlarging swelling over a 7-month period in the submandibular region, clinically resembling a salivary gland tumor. There were no associated marginal mandibular, lingual, or hypoglossal nerve palsy. The mass was excised completely, and histopathological examination revealed it to be a schwannoma. It is appropriate to consider benign peripheral nerve tumors, such as schwannoma, in the differential diagnosis of submandibular space tumors in children.
  16. Elmahrouk A, Daoulah A, Panduranga P, Rajan R, Jamjoom A, Kanbr O, et al.
    Int J Surg, 2024 Aug 08;110(11):7142-9.
    PMID: 39116452 DOI: 10.1097/JS9.0000000000002032
    BACKGROUND: Risk stratification for patients undergoing coronary artery bypass surgery (CABG) for left main coronary artery (LMCA) disease is essential for informed decision-making. This study explored the potential of machine learning (ML) methods to identify key risk factors associated with mortality in this patient group.

    METHODS: This retrospective cohort study was conducted on 866 patients from the Gulf Left Main Registry who presented between 2015 and 2019. The study outcome was hospital all-cause mortality. Various machine learning models [logistic regression, random forest (RF), k-nearest neighbor, support vector machine, naïve Bayes, multilayer perception, boosting] were used to predict mortality, and their performance was measured using accuracy, precision, recall, F1 score, and area under the receiver operator characteristic curve (AUC).

    RESULTS: Nonsurvivors had significantly greater EuroSCORE II values (1.84 (10.08-3.67) vs. 4.75 (2.54-9.53) %, P<0.001 for survivors and nonsurvivors, respectively). The EuroSCORE II score significantly predicted hospital mortality (OR: 1.13 (95% confidence interval: 1.09-1.18), P<0.001), with an AUC of 0.736. RF achieved the best ML performance (accuracy=98, precision=100, recall=97 and F1 score=98). Explainable artificial intelligence using SHAP demonstrated the most important features as follows: preoperative lactate level, emergency surgery, chronic kidney disease (CKD), NSTEMI, nonsmoking status, and sex. QLattice identified lactate and CKD as the most important factors for predicting hospital mortality this patient group.

    CONCLUSION: This study demonstrates the potential of ML, particularly the Random Forest, to accurately predict hospital mortality in patients undergoing CABG for LMCA disease and its superiority over traditional methods. The key risk factors identified, including preoperative lactate levels, emergency surgery, chronic kidney disease, NSTEMI, nonsmoking status, and sex, provide valuable insights for risk stratification and informed decision-making in this high-risk patient population. Additionally, incorporating newly identified risk factors into future risk scoring systems can further improve mortality prediction accuracy.

  17. Xu XJ, Myint PK, Lee SWH, Ramasamy K, Lim SM, Majeed ABA, et al.
    Int J Clin Pharm, 2024 Dec;46(6):1453-1463.
    PMID: 39264491 DOI: 10.1007/s11096-024-01791-1
    BACKGROUND: While the effects of anticholinergic drug use have been increasingly highlighted, trends in anticholinergic use remain poorly understood.

    AIM: To determine the changes in frequency and pattern of anticholinergic drug use within a low- and middle-income country.

    METHOD: Comparisons were made between population-based datasets collected from Malaysian residents aged 55 years and older in 2013-15 and 2020-22. Anticholinergic exposure was determined using the anticholinergic cognitive burden (ACB) tool. Drugs with ACB were categorised according to the Anatomical Therapeutic Chemical (ATC) classification.

    RESULTS: A total number of 5707 medications were recorded from the 1616 participants included in the 2013-15 dataset. A total number of 6175 medications were recorded from 2733 participants in 2020-22. Two hundred and ninety-three (18.1%) and 280 (10.2%) participants consumed ≥ 1 medication with ACB ≥ 1 in 2013-15 and 2020-22 respectively. The use of nervous system drugs with ACB had increased (27 (0.47%) versus 39 (0.63%). The use of ACB drugs in the cardiovascular (224 (3.9%) versus 215 (3.4%)) and alimentary tract and metabolism (30 (0.52%) versus 4 (0.06%)) classes had reduced over time. Participants in 2020-22 were significantly less likely than those in 2013-15 to have total ACB = 1 - 2 (odds ratio [95% confidence interval] = 0.473[0.385-0.581]) and ACB ≥ 3 (0.251[0.137 - 0.460]) compared to ACB = 0 after adjustment for potential confounders (p 

    MeSH terms: Aged; Aged, 80 and over; Developing Countries; Drug Utilization/trends; Drug Utilization/statistics & numerical data; Female; Humans; Longitudinal Studies; Malaysia/epidemiology; Male; Middle Aged
  18. Teo HL, Abdul Wahab R, Zainal-Abidin MH, Mark-Lee WF, Susanti E
    Int J Biol Macromol, 2024 Nov;280(Pt 2):135787.
    PMID: 39304051 DOI: 10.1016/j.ijbiomac.2024.135787
    This study explores an eco-friendly delignification technique for raw oil palm leaves (OPL), highlighting the optimized conditions of choline chloride-lactic acid deep eutectic solvent (DES)-mediated ball milling pretreatment to maximize the co-production yields of highly crystalline cellulose and lignin. Our five-level-four-factor Taguchi design identified the optimal reaction settings for cellulose production (85.83 % yield, 47.28 % crystallinity) as 90-minute milling, 1500 rpm, mill-ball size ratio of 30:10, ball-to-sample mass ratio of 20:1, DES-to-sample mass ratio of 3:1. Conversely, the maximal lignin extraction yield (35.23 %) occurred optimally at 120-minute milling, 600 rpm, mill-ball size ratio of 25:5, ball-to-sample mass ratio of 20:1 and DES-to-sample mass ratio of 9:1. Statistical results showed that milling frequency (p-value ≤ 0.0001) was highly significant in improving cellulose crystallinity and yield, while DES-to-sample mass ratio (p-value ≤ 0.0001) was the most impacting on lignin yield. The thermogravimetric method affirmed the elevated cellulose thermal stability, corroborating the enhanced cellulose content (40.14 % to 73.67 %) alongside elevated crystallinity and crystallite size (3.31 to 4.72 nm) shown by X-ray diffractograms. The increased surface roughness seen in micrographs mirrored the above-said post-treatment changes. In short, our optimized one-pot dual-action pretreatment effectively delignified the raw OPL to produce cellulose-rich material with enhanced crystallinity and lignin solidity.
    MeSH terms: Solvents/chemistry; Arecaceae/chemistry
  19. Nordin AH, Yusoff AH, Husna SMN, Noor SFM, Norfarhana AS, Paiman SH, et al.
    Int J Biol Macromol, 2024 Nov;280(Pt 2):135799.
    PMID: 39307484 DOI: 10.1016/j.ijbiomac.2024.135799
    The long-term presence of pharmaceutical pollution in water bodies has raised public awareness. Nanocellulose is often used in adsorption to remove pollutants from wastewater since it is an abundant, green and sustainable material. This paper offers an extensive overview of the recent works reporting the potential of nanocellulose-based adsorbents to treat pharmaceutical wastewater. This study distinguishes itself by not only summarizing recent research findings but also critically integrating discussions on the improvements in nanocellulose production and sorts of alterations based on the type of pharmaceutical contaminants. Commonly, charged, or hydrophobic characteristics are introduced onto nanocellulose surfaces to accelerate and enhance the removal of pharmaceutical compounds. Although adsorbents based on nanocellulose have considerable potential, several significant challenges impede their practical application, particularly concerning cost and scalability. Large-scale synthesis of nanocellulose is technically challenging and expensive, which prevents its widespread use in wastewater treatment plants. Continued innovation in this area could lead to breakthroughs in the practical application of nanocellulose as a superior adsorbent. The prospects of utilization of nanocellulose are explained, providing a sustainable way to address the existing restriction and maximize the application of the modified nanocellulose in the field of pharmaceutical pollutants removal.
    MeSH terms: Adsorption; Pharmaceutical Preparations/isolation & purification; Pharmaceutical Preparations/chemistry; Nanostructures/chemistry
  20. Chellathurai MS, Chung LY, Hilles AR, Sofian ZM, Singha S, Ghosal K, et al.
    Int J Biol Macromol, 2024 Nov;280(Pt 2):135775.
    PMID: 39307491 DOI: 10.1016/j.ijbiomac.2024.135775
    Chitosan (CS) has become a focal point of extensive research in the pharmaceutical industry due to its remarkable biodegradability, biocompatibility and sustainability. Chitosan hydrogels (CS HGs) are characterized by their viscoelasticity, flexibility and softness. The polar surfaces exhibit properties that mitigate interfacial tension between the hydrogel and body fluids. The inherent compatibility of CS HGs with body tissues and fluids positions them as outstanding polymers for delivering therapeutic proteins, peptides, DNA, siRNA, and vaccines. Designed to release drugs through mechanisms such as swelling-based diffusion, bioerosion, and responsiveness to stimuli, CS HGs offer a versatile platform for drug delivery. CS HGs play pivotal roles in serving purposes such as prolonging the duration of preprogrammed drug delivery, enabling stimuli-responsive smart delivery to target sites, protecting encapsulated drugs within the mesh network from adverse environments, and facilitating mucoadhesion and penetration through cell membranes. This review comprehensively outlines various novel preparation methods of CS HGs, delving into the parameters influencing drug delivery system design, providing a rationale for CS HG utilization in drug delivery, and presenting diverse applications across the pharmaceutical landscape. In synthesizing these facets, the review seeks to contribute to a nuanced understanding of the multifaceted role that CS HGs play in advancing drug delivery methodologies.
    MeSH terms: Animals; Biocompatible Materials/chemistry; Drug Carriers/chemistry; Humans; Drug Delivery Systems
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