Browse publications by year: 2024

  1. Kuan WC, Lim KK, Chee KH, Kasim S, Dujaili JA, Lee KK, et al.
    Qual Life Res, 2024 Oct 30.
    PMID: 39476200 DOI: 10.1007/s11136-024-03818-6
    PURPOSE: This study aimed to examine the trajectory in health-related quality of life (HRQoL) during and after hospitalisation for worsening of heart failure (HF) in Malaysia.

    METHODS: 200 patients with heart failure and reduced ejection fraction (HFrEF) admitted into two hospitals in Malaysia due to worsening of HF were surveyed using the EQ-5D-5 L questionnaire. The primary outcomes were utility values at admission, discharge and 1-month post-discharge (1MPD). Secondary outcomes included the visual analogue scores (VAS) and the proportion of patients reporting each EQ-5D-5 L dimension levels. Missing data were imputed using multiple imputation, and generalised linear mixed models were fitted.

    RESULTS: At admission, the unadjusted mean utility values and VAS scores for HFrEF patients in Malaysia were as low as 0.150 ± 0.393 and 38.2 ± 20.8, respectively. After a median hospital stay of 4 days, there was a significant improvement in utility values and VAS scores by 0.510 (95% CI: 0.455-0.564) and 28.8 (95% CI: 25.5-32.1), respectively. The utility value and VAS score at 1-month post-discharge were not significantly different from discharge. The proportion of HFrEF patients reporting problems and severe problems in mobility, self-care, usual activities, and anxiety/depression, pain/discomfort reduced at varying degree from admission to discharge and 1MPD.

    CONCLUSION: HF is a progressive condition with substantial variation in HRQoL during the disease trajectory. During hospitalisation due to worsening of HF, HFrEF population has unfavourable HRQoL. Rapid and significant HRQoL improvement was observed at discharge, which sustained over one month. The study findings can inform future cost-effectiveness analyses and policies.

  2. Khan J, Rezo V, Vincze T, Weis M, Momin SA, El-Atab N, et al.
    Chemosphere, 2024 Nov;367:143618.
    PMID: 39490758 DOI: 10.1016/j.chemosphere.2024.143618
    Nitrogen dioxide (NO2) is a major cause of respiratory disorders in outdoor and indoor environments. Real-time NO2 monitoring using nonintrusive wearable devices can save lives and provide valuable health data. This study reports a room-temperature, wearable, and flexible smart NO2 gas sensor fabricated via cost-effective printing technology on a polyimide substrate. The sensor uses alkali lignin with edge-oxidised graphene oxide (EGO-AL) ink, demonstrating a sensitivity of 1.70% ppm⁻1 and a detection limit of 12.70 ppb, with excellent selectivity towards NO2. The high sensing properties are attributed to labile oxygen functional groups from GO and alkali lignin, offering abundant interacting sites for NO2 adsorption and electron transfer. The sensor fully recovers to the baseline after heat treatment at 150 °C, indicating its reusability. Integration into lab coats showcased its wearable application, utilising a flexible printed circuit board to wirelessly alert the wearer via cell phone to harmful NO2 levels (>3 ppm) in the environment. This smart sensing application underscores the potential for practical, real-time air quality monitoring, personal safety enhancement, and health management.
    MeSH terms: Environmental Monitoring/instrumentation; Environmental Monitoring/methods; Humans; Ink; Oxides/chemistry; Limit of Detection
  3. Khairul-Asri M G, Jaharudin M EA, Khor V KV, Yusof M R, Mohamad Sharin M F, Jagwani A JA, et al.
    Urologiia, 2024 Sep.
    PMID: 39563589
    OBJECTIVE: To compare the tolerability and feasibility of transrectal(TR) versus transperineal (TP) routes for prostate biopsy under local anaesthesia(LA). To assess the functional outcome and the complication of both procedures.

    METHOD: s. A prospective cohort observational study was performed on patients who underwent prostate biopsy under LA. Visual Analogue Scale (VAS) was used during the procedure. International Prostate Symptoms Score (IPSS) and International Index of Erectile dysfunction (IIEF) were assessed before the procedure and in 14 days after the procedure. Complication for each procedure was recorded.

    RESULT: A total of 128 patients with 64 patients for each group underwent prostate biopsy by TP and TR under LA. TP targeted biopsy group had comparable pain scores to those who underwent the procedure using the TR routes. The median pain score for the TP group was 2 and TR was 3, (IQR=2, range 0-10 for both groups)with no significant pain difference between both groups (P=0.48). Furthermore, there was no significant difference in urinary function(p=0.68) and sexual function (p=0.19) between the two groups post-procedure. Both groups have similar rates of complications, with no significant difference observed. Urinary tract infection incidents that did occur were rare and did not significantly differ between the groups (p=0.21). None of the patients experienced sepsis postoperatively. AUR was reported in both groups, slightly higher with 9.4%(N=6) in the TP group and 6.3%(N=4) in the TR group however no significant difference(p=0.112) was noted. Haematuria is common in both groups with TP (66%) and TR (59%) but self-limiting with Clavien-Dindo grade I without significant difference (p=0.589).

    CONCLUSION: Our results showed that both Transperineal and transrectal approaches have similar tolerability with no significant difference in functional outcome or complications. Further studies are mandatory to verify our results.

    MeSH terms: Aged; Humans; Magnetic Resonance Imaging/methods; Male; Middle Aged; Perineum; Prospective Studies; Prostatic Neoplasms/pathology; Prostatic Neoplasms/surgery; Cohort Studies; Ultrasonography, Interventional/methods; Patient Reported Outcome Measures
  4. Wang X, Rasidi WNA, Seluakumaran K
    Int J Audiol, 2024 Nov 20.
    PMID: 39563635 DOI: 10.1080/14992027.2024.2429025
    OBJECTIVES: This two-part pilot study investigated the feasibility of using a frequency selectivity measure (TFS) to develop a novel hearing screening test for cooperative adults. Study 1 determined the optimal masker level, while Study 2 assessed the reliability of a self-administered TFS test prototype performed at the selected masker level.

    DESIGN AND STUDY SAMPLE: Study 1 (normal hearing, n = 20) examined masker-level effects on TFS using a manual threshold determination method from an earlier study. Study 2 (normal hearing, n = 21; hearing loss, n = 5) evaluated the reliability of a self-administered TFS test using a new automated threshold determination procedure.

    RESULTS: Moderate masker levels (30-40 dB SPL) were suitable for the TFS measurements, with 40 dB SPL being optimal. Lower level (20 dB SPL) led to floor effects, while higher level (50 dB SPL) broadened cochlear tuning and reduced TFS values. The self-administered test demonstrated ±9 dB limit of agreement, with intra-subject absolute mean differences of 1.8-2.7 dB across test frequencies, indicating greater variability compared to the manual method.

    CONCLUSIONS: The self-administered TFS test is a candidate for hearing screening, particularly for mild sensorineural hearing loss. However, further research is needed to reduce measurement variability and optimise testing for real-world use.

  5. Huan NC, Ng KL, Nyanti LE, Khaw JY, Lee JH, Mohd Aminudin NH, et al.
    Respirol Case Rep, 2024 Nov;12(11):e70061.
    PMID: 39563685 DOI: 10.1002/rcr2.70061
    A third of patients with non-Hodgkin's lymphoma (NHL) develop pleural effusion during the disease course for various reasons. In most cases, lymphoma-related pleural effusion is a manifestation of widespread systemic disease, signifying a high tumour burden and therefore, a poorer prognosis. On the other hand, primary pleural lymphomas (PPLs) exhibit exclusive or dominant involvement of serous cavities, without detectable solid tumour masses. PPL is an uncommon disease and is of two types: primary effusion lymphoma (PEL) and diffuse large B-cell lymphoma associated with chronic inflammation (DLBCL-CI). PPLs not related to PELs and DLBCL-CIs are exceedingly rare. Herein, we describe four patients with biopsy proven B-cell NHL. One had no extra-pleural involvement at the time of diagnosis, indicating PPL. In all cases, histopathological examination of pleural biopsies obtained via medical thoracoscopy (MT) were crucial in clinching the final diagnosis. Clinicians are alerted to the potential relationship between exudative effusion and NHL as well as the role of MT in the diagnosis of B-cell NHL.
  6. Bhat S, Gunawardana DU, Boparai DK, Bamunusinghe TK, Krishanth K, Premakrishna A, et al.
    PMID: 39563920 DOI: 10.1017/ash.2024.419
    BACKGROUND: During the past several decades, enterococci are emerging as an important cause of healthcare-associated infections. They have developed resistance to various antimicrobials previously used for the treatment of urinary tract infections (UTIs). This study was aimed to determine the prevalence of Enterococcus species among urinary tract-infected patients in a tertiary care hospital, in Karnataka, India.

    MATERIAL AND METHODS: We have analyzed 4341 culture-positive urine samples received by microbiology laboratory during the year 2021. The bacterial identification was done by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The antibiotic sensitivity was tested by automated VITEK-2® COMPACT (bioMérieux) system.

    RESULTS: Among 4341 culture-positive samples, Enterococcal species were isolated from 159 samples. A total of 64.7% of the isolates were identified as Enterococcus faecalis and 28.3% of the strains as Enterococcus faecium. All the enterococci were sensitive to linezolid, teicoplanin, and vancomycin, whereas 59.1%, 30.9%, and 23.3% of the strains exhibited resistance to high-level gentamicin, benzylpenicillin, and nitrofurantoin, respectively. 33.67 % of the isolates were identified as multidrug-resistant (MDR) strains as they exhibited resistance to high-level gentamicin, benzylpenicillin, and nitrofurantoin.

    CONCLUSION: Our study shows the prevalence of Enterococcus faecalis and high-level gentamicin-resistant enterococcal strains. The MDR pattern of enterococci requires careful consideration of antimicrobial therapy to treat UTIs. The reserved drugs such as linezolid, vancomycin, and teicoplanin should be cautiously used for the treatment of enterococcal UTI.

  7. Yee Mun H, Alawi R, Abdul Muttlib NA, Abd Ghani H, Noorani TY
    Cureus, 2024 Oct;16(10):e71950.
    PMID: 39564065 DOI: 10.7759/cureus.71950
    Iatrogenic perforation is caused by the degradation of the dentin root floor or wall and the overlying cementum. This condition frequently arises from improper post-space preparation, root canal negotiation and preparation, and the use of misaligned burs or mechanical instruments during endodontic access. Proper management is essential; otherwise, it could lead to the need for tooth extraction. Management of iatrogenic perforation can be challenging. In this case report, a strip perforation on an upper anterior tooth was addressed for tooth rehabilitation using tricalcium silicate-based cement and a flexible glass fiber post. This approach aims to restore the tooth's function, structural integrity, and aesthetics while minimizing complications.
  8. Ghazali KC, Mat AD, Yaacob H, Hamdan MUO, Sidek ASM
    J Surg Case Rep, 2024 Nov;2024(11):rjae711.
    PMID: 39564073 DOI: 10.1093/jscr/rjae711
    Pulmonary hypertension is a known perioperative risk factor that carries a high morbidity and mortality rate. Severe pulmonary hypertension is related to high morbidity after general anaesthesia. We are reporting three patients with underlying severe pulmonary hypertension, who presented with intestinal obstruction managed with different perioperative approaches. In case 1, a 38-year-old man with Eisenmenger syndrome and severe pulmonary hypertension underwent exploratory laparotomy, right hemicolectomy, and double barrel stoma for obstructed right-sided colonic tumour. He passed away on Day 6 post-operation. In case 2, a 52-year-old man with Eisenmenger syndrome and severe pulmonary hypertension presented with obstructed rectosigmoid tumour and jejunojejunal intussusception and underwent exploratory laparotomy and Hartmann's procedure. He succumbed after 33 days of fighting with cardiovascular and respiratory complications. In case 3, a 65-year-old woman, with strangulated paraumbilical hernia, underwent mini laparotomy, small bowel resection, primary anastomosis, and paraumbilical hernia repair under monitored sedation and local anaesthesia. She was discharged home after 7 days of hospitalization.
  9. Othin M, Haranal M, Sivalingam S, Khalid KFM, Soo KW
    Ann Pediatr Cardiol, 2024;17(3):180-187.
    PMID: 39564156 DOI: 10.4103/apc.apc_66_24
    BACKGROUND: Extracorporeal membrane oxygenation (ECMO) is a vital mechanical circulatory support used with increasing frequency in complex congenital cardiac surgeries. This study evaluated the outcomes of a protocol-based venoarterial (VA) ECMO program following congenital heart surgeries.

    METHODS AND RESULTS: This was a retrospective review of 198 patients who underwent VA-ECMO after congenital cardiac surgeries at our institute between 2004 and 2023. Patients were divided into pre-ECMO protocol (2004-2017) and post-ECMO protocol (2018-2023) implementation. There were 107 patients in the preprotocol era and 91 in the postprotocol era. We compared weaning from ECMO and survival to hospital discharge between the two eras. An analysis of the factors influencing survival to hospital discharge was also done. ECMO was initiated through the central cannulation technique through median sternotomy in all patients. The median age and weight at initiation were 4 months (interquartile range [IQR] 1-33.5 months) and 4.4 kg (IQR 3.3-10.1 kg), respectively. The successful weaning of the ECMO (n = 67/91, 73.6%) and survival to discharge (n = 43/91, 47.3%) were higher in patients of the postprotocol era. However, it was not statistically significant. Higher risk adjustment for congenital heart surgery-1 >3 and acute kidney injury were independent predictors of poorer survival to hospital discharge.

    CONCLUSIONS: A protocol-based ECMO program may improve outcomes of successful weaning and survival to discharge in patients undergoing congenital cardiac surgeries.

  10. Ross JL, Teeraananchai S, Avihingsanon A, Lee MP, Ditangco R, Rajasuriar R, et al.
    J Acquir Immune Defic Syndr, 2024 Aug 15;96(5):421-428.
    PMID: 39564477 DOI: 10.1097/qai.0000000000003446
    BACKGROUND: Mental health and substance use disorders are common among people living with HIV and are associated with high-risk sexual behaviors, such as unprotected sex and multiple sexual partners, but Asia-Pacific data are limited.

    METHODS: Adult PLHIV in care at five Asia-Pacific HIV clinics were enrolled at routine clinic visits between July 2019 and June 2020. Depression, substance use, sexual practice and socio-demographic data were collected using PHQ-9, ASSIST, and a study-specific questionnaire. Clinical data were accessed from medical records. Risk factors for medium- to high-risk sexual practices, defined based on total scores from the sexual practice questionnaire assessing number of sexual partners and condom use, were analyzed using logistic regression. Moderate to severe depression was defined as a PHQ-9 score >9, and moderate- to high-risk substance use as an ASSIST score ≥11 for alcohol or ≥4 for other substances.

    RESULTS: Among 723 participants, median age was 38 years, 89% were male, 99% were on ART and 37% had medium- to high-risk sexual practices. Medium- to high-risk sexual practices were more common among those ≤30 years old, unemployed, and HIV status disclosed, and were more likely in participants with moderate to severe depression (aOR 2.09, 95%CI 1.17-3.74) compared to none to minimal depression, and moderate- to high-risk substance use (aOR 1.73, 95%CI 1.23-2.44) compared to those without.

    CONCLUSIONS: Further integration of comprehensive sexual risk reduction strategies, mental health services and substance use harm reduction within HIV clinical settings in the region is needed.

    MeSH terms: Adult; Asia/epidemiology; Female; Humans; Male; Middle Aged; Surveys and Questionnaires; Risk Factors; Risk-Taking*; Sexual Behavior*; Sexual Partners; Unsafe Sex/statistics & numerical data; Young Adult
  11. Sattar K, Meo SA, Yusoff MSB
    Front Med (Lausanne), 2024;11:1468654.
    PMID: 39564509 DOI: 10.3389/fmed.2024.1468654
    INTRODUCTION: The rigorous nature of medical education, long and night shifts, and prevalent issues like stress, anxiety, and depression affect medical students' mental well-being and medical professionalism. This study aims to explore the intricate relationships between mental well-being, medical professionalism, and coping strategies, among undergraduate medical students, utilizing structural equation modeling (SEM) to unravel these dynamics.

    METHODS: Conducted at Universiti Sains Malaysia, this cross-sectional study involved 234 medical students from the 1st, 3rd, and 5th years of the MBBS program. Data were collected via five validated survey instruments: DASS-9, TEQ, Dundee, Brief COPE, and CBI, through Google Forms. Participants were selected using purposive sampling. The surveys assessed mental well-being (burnout, anxiety, depression, stress), coping strategies, and medical professionalism attributes. Model fit was evaluated using established indices.

    RESULTS: Findings indicated that professional behavior reduces burnout and negatively impacts negative coping strategies (NCSs). Additionally, medical professionalism indirectly enhances empathy and positively influences CSs. Conversely, psychological distress increases NCSs and reduces empathy. Positive coping strategies (PCSs) enhance empathy levels, while MWB issues elevate NCSs.

    DISCUSSION: The study underscores the vital role of professional behavior in mitigating burnout and fostering positive coping mechanisms among medical students. Addressing MWB issues through targeted interventions can enhance empathy and professional behavior, ultimately improving the quality of patient care.

  12. Chisholm RA, Fung T, Anderson-Teixeira KJ, Bourg NA, Brockelman WY, Bunyavejchewin S, et al.
    Proc Biol Sci, 2024 Nov;291(2035):20240486.
    PMID: 39564678 DOI: 10.1098/rspb.2024.0486
    Populations of forest trees exhibit large temporal fluctuations, but little is known about the synchrony of these fluctuations across space, including their sign, magnitude, causes and characteristic scales. These have important implications for metapopulation persistence and theoretical community ecology. Using data from permanent forest plots spanning local, regional and global spatial scales, we measured spatial synchrony in tree population growth rates over sub-decadal and decadal timescales and explored the relationship of synchrony to geographical distance. Synchrony was high at local scales of less than 1 km, with estimated Pearson correlations of approximately 0.6-0.8 between species' population growth rates across pairs of quadrats. Synchrony decayed by approximately 17-44% with each order of magnitude increase in distance but was still detectably positive at distances of 100 km and beyond. Dispersal cannot explain observed large-scale synchrony because typical seed dispersal distances (<100 m) are far too short to couple the dynamics of distant forests on decadal timescales. We attribute the observed synchrony in forest dynamics primarily to the effect of spatially synchronous environmental drivers (the Moran effect), in particular climate, although pests, pathogens and anthropogenic drivers may play a role for some species.
    MeSH terms: Population Dynamics*; Seed Dispersal; Forests*
  13. Nusurupia JJ, Germana LH, Wickramasinghe P, Tang HK, Munambah N, Hossain MS, et al.
    Child Care Health Dev, 2024 Nov;50(6):e70008.
    PMID: 39564734 DOI: 10.1111/cch.70008
    BACKGROUND: Insufficient physical activity, excessive screen time and short sleep duration among young children are global public health concerns; however, data on prevalence of meeting World Health Organisation 24-h movement behaviour guidelines for 3-4-year-old children in low- and middle-income countries (LMICs) are limited, and it is unknown whether urbanisation is related to young children's movement behaviours. The present study examined differences in prevalence of meeting 24-h movement behaviour guidelines among 3-4-year-old children living in urban versus rural settings in LMICs.

    METHODS: The SUNRISE Study recruited 429, 3-4-year-old child/parent dyads from 10 LMICs. Children wore activPAL accelerometers continuously for at least 48 h to assess their physical activity and sleep duration. Screen time and time spent restrained were assessed via parent questionnaire. Differences in prevalence of meeting guidelines between urban- and rural-dwelling children were examined using chi-square tests.

    RESULTS: Physical activity guidelines were met by 17% of children (14% urban vs. 18% rural), sleep guidelines by 57% (61% urban vs. 54% rural), screen time guidelines by 50% (50% urban vs. 50% rural), restrained guidelines by 84% (81% urban vs. 86% rural) and all guidelines combined by 4% (4% urban vs.4% rural). We found no significant differences in meeting the guidelines between urban and rural areas.

    CONCLUSIONS: Only a small proportion of children in both rural and urban settings met the WHO 24-h movement guidelines. Strategies to improve movement behaviours in LMICs should consider including both rural and urban settings.

    MeSH terms: Child Behavior; Child, Preschool; Developing Countries*; Female; Humans; Male; Pilot Projects; Rural Population*; Sleep/physiology; Urban Population*; Exercise*; Guideline Adherence/statistics & numerical data; Sedentary Lifestyle*; Accelerometry
  14. Ten KE, Rahman S, Tan HS
    Microb Genom, 2024 Nov;10(11).
    PMID: 39565092 DOI: 10.1099/mgen.0.001327
    Despite being a major human pathogen, limited studies have reported RNA modifications in Acinetobacter baumannii. These post-transcriptional modifications play crucial regulatory roles in bacteria and have also been shown to modulate bacterial virulence. Using nanopore sequencing, we characterized RNA modifications in a virulent A. baumannii strain (Ab-C98) under free-living (mid-exponential phase in vitro culture) and during an early stage of infection (3 h post-infection) in Galleria mellonella larvae. Analysis revealed that m5C methylations are essential for ribosome synthesis, while m6A and Ψ are involved in metabolic pathways and translation processes. Iron-chelating genes exbD (m5C and m6A) and feoB (m6A and Ψ) and RNA polymerase subunit rpoC (m6A and Ψ) were selectively modified during infection. This first transcriptome-wide study highlights the potential regulatory roles of m5C, m6A and Ψ modifications in A. baumannii during infection.
    MeSH terms: Acinetobacter Infections/microbiology; Animals; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Larva/microbiology; Moths/microbiology; RNA Processing, Post-Transcriptional*; RNA, Bacterial/genetics; Virulence/genetics; Gene Expression Regulation, Bacterial; Transcriptome*
  15. Turkyilmaz I, Abdullah JY, Wilkins GN, Bernardi S, Varvara G
    Minerva Dent Oral Sci, 2024 Nov 20.
    PMID: 39565339 DOI: 10.23736/S2724-6329.24.04898-8
    BACKGROUND: The purpose of this study was to determine the accuracy of an intraoral scanner to digitally duplicate complete dentures and analyze the effects of mesh reduction of digital files on the surface area and volume accuracy of complete dentures, in vitro.

    METHODS: A mandibular complete denture was scanned by a desktop scanner to create a digital STL reference file (control). Fifteen identical scans were created by using an intraoral scanner and exported as STL files (test group). These 15 files were saved at 100% of the original scan resolution then reduced to 75%, 50%, 25%, and 10% of their original quality. These 75 scans were statistically analyzed by calculating The Hausdorff Distance (HD) and Dice Similarity Coefficients (DSC) to assess the variation between the mean reduced intraoral scanner files test and the control desktop scanner file and eventual inconsistencies. The volumes of the reduced mesh files were also compared with the 100% resolution intraoral mesh files to evaluate precision and trueness of the intraoral scanner.

    RESULTS: Reduced mesh files of 10%, 25%, 50%, 75% of the original scan yielded a percentage similarity average of 99.7%, indicating a very high precision value for the intraoral scanner. Also, the volumes of each associated mesh reduction slightly decreased with non-statistically significant results.

    CONCLUSIONS: This study concluded that the chosen intraoral scanner for this study provided very high trueness (98.34%) and precision (99.7%), and also the volumes of reduced mesh files slightly decreased but were not statistically significant.

  16. Jabri T, Daalah M, Alawfi BS, Gul J, Ahmed U, Shah MR, et al.
    Parasitol Res, 2024 Nov 20;123(11):387.
    PMID: 39565414 DOI: 10.1007/s00436-024-08389-6
    Acanthamoeba castellanii is the causative pathogen of a severe eye infection, known as Acanthamoeba keratitis and a life-threatening brain infection, named granulomatous amoebic encephalitis. Current treatments are problematic and costly and exhibit limited efficacy against Acanthamoeba parasite, especially the cyst stage. In parallel to drug discovery and drug repurposing efforts, drug modification is also an important approach to tackle infections, especially against neglected parasites such as free-living amoebae: Acanthamoeba. In this study, we determined whether modifying pentamidine and doxycycline through chitosan-functionalized graphene oxide loading enhances their anti-amoebic effects. Various concentrations of doxycycline, pentamidine, graphene oxide, chitosan-functionalized graphene oxide, and chitosan-functionalized graphene oxide loaded with doxycycline and pentamidine were investigated for amoebicidal effects against pathogenic A. castellanii belonging to the T4 genotype. Lactate dehydrogenase assays were performed to determine toxic effects of these various drugs and nanoconjugates against human cells. The findings revealed that chitosan-functionalized graphene oxide loaded with doxycycline demonstrated potent amoebicidal effects. Nanomaterials significantly (p 
    MeSH terms: Amebicides/pharmacology; Humans
  17. Asghar MA, Tang S, Wong LP, Yang P, Zhao Q
    J Ophthalmic Inflamm Infect, 2024 Nov 20;14(1):60.
    PMID: 39565496 DOI: 10.1186/s12348-024-00444-8
    BACKGROUND: Infectious uveitis is a significant cause of visual impairment worldwide, caused by diverse pathogens such as viruses, bacteria, fungi, and parasites. Understanding its prevalence, etiology, pathogenesis, molecular mechanism, and clinical manifestations is essential for effective diagnosis and management.

    METHODS: A systematic literature search was conducted using PubMed, Google Scholar, Web of Science, Scopus, and Embase, focusing on studies published in the last fifteen years from 2009 to 2023. Keywords included "uveitis," "infectious uveitis," "viral uveitis," and others. Rigorous inclusion and exclusion criteria were applied, and data were synthesized thematically. Gene symbols related to infectious uveitis were analyzed using protein-protein interaction (PPI) networks and pathway analyses to uncover molecular mechanisms associated with infectious uveitis.

    RESULTS: The search from different databases yielded 97 eligible studies. The review identified a significant rise in publications on infectious uveitis, particularly viral uveitis, over the past fifteen years. Infectious uveitis prevalence varies geographically, with high rates in developing regions due to systemic infections and limited diagnostic resources. Etiologies include viruses (39%), bacteria (17%), and other pathogens, substantially impacting adults aged 20-50 years. Pathogenesis involves complex interactions between infectious agents and the ocular immune response, with key roles for cytokines and chemokines. The PPI network highlighted IFNG, IL6, TNF, and CD4 as central nodes. Enriched pathways included cytokine-cytokine receptor interaction and JAK-STAT signaling. Clinical manifestations range from anterior to posterior uveitis, with systemic symptoms often accompanying ocular signs. Diagnostic strategies encompass clinical evaluation, laboratory tests, and imaging, while management involves targeted antimicrobial therapy and anti-inflammatory agents.

    CONCLUSION: This review underscores the complexity of infectious uveitis, driven by diverse pathogens and influenced by various geographical and systemic factors. Molecular insights from PPI networks and pathway analyses provide a deeper understanding of its pathogenesis. Effective management requires comprehensive diagnostic approaches and targeted therapeutic strategies.

  18. Loganathan T, Chan ZX, Hassan F, Kunpeuk W, Suphanchaimat R, Yi H, et al.
    PLoS One, 2024;19(11):e0314491.
    PMID: 39565764 DOI: 10.1371/journal.pone.0314491
    [This corrects the article DOI: 10.1371/journal.pone.0259546.].
  19. Abbasi SA, Rehman W, Rahim F, Hussain R, Hawsawi MB, Alluhaibi MS, et al.
    PMID: 39565952 DOI: 10.1515/znc-2024-0202
    Diabetes mellitus (DM) is a disorder which is raised at the alarming level and it is characterized by the hyperglycemia results from the impaired action of insulin, production of insulin or both of these simultaneously. Consequently, it causes problems or failure of different body organs such as kidneys, heart, eyes, nerve system. Since this disease cannot be completely cured until now, we aimed to design series of enzymes inhibitors and tested them for DM treatment. In this series, benzimidazole-based thiazolidinone bearing chalcone derivatives completed in a four step reaction and their structures were confirmed through various spectroscopic techniques. A significant efficacy on antidiabetic enzymes was observed, with IC50 values ranging from 25.05 ± 0.04 to 56.08 ± 0.07 μM for α-amylase and 22.07 ± 0.02 to 53.06 ± 0.07 μM for α-glucosidase. The obtained results were compared to those of the standard glimepiride drug (IC50 = 18.05 ± 0.07 µM for α-amylase and IC50 = 15.02 ± 0 .03 µM for α-glucosidase). The synthesized compounds showed promising antidiabetic potency. Moreover, a molecular docking study was conducted on the most active analogs of the compounds to better understand their interactions with the active sites of the targeted enzymes.
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