Browse publications by year: 2000

  1. Yusof RM, Haque F, Ismail M, Hassan Z
    Asia Pac J Clin Nutr, 2000 Jun;9(2):130-5.
    PMID: 24394399
    Probiotic organism Bifidobacteria was isolated from the faeces of breast-fed infants at Universiti Putra Malaysia. Trypticase phytone peptone yeast extract agar (TPY) was used as a selective media for the isolation. Morphological examination of the isolates indicated that Bifidobacteria was Gram-positive rods in nature, curved with characteristics of V and Y shapes. The organisms were non-catalase producing, non-nitrate reducing, non-motile, had an absence of indole and were unable to liquify gelatin. The ratios of acetic and lactic acids were determined using high performance liquid chromatography (HPLC). Using carbohydrate fermentation profile test API-CH-50 kits, 20 Bifidobacteria strains had been identified: they were the species of Bifidobacteria infantis and two different sub-species, mainly infantis and lacentis. Based on a wide zone of inhibition, three suitable strains of B. infantis, Bifi-11, Bifi-19 and Bifi-20, were tested in weaning foods for antimicrobial activity towards two human pathogens: E. coli-0157 (World Health Organization) and Salmonella typhimurium S-285. The pH, titratable acidity of weaning foods and total colony count for Bifidobacteria, enteropathogenic Escherichia coli and S. typhimurium were recorded at 3-h intervals for 30 h. It was found that after 9 h of incubation of weaning foods, the pH declined to < 3.6 from pH 6.0, whereas titratable acidity increased from 0.026 to 0.08%. It was indicated that Bifidobacteria inhibited E. coli better than did S typhimurium due to low pH. After 24 h of incubation, approximately 98% of E. coli was inhibited by Bifidobacteria. It is suggested that the inhibitory effect of Bifidobacteria strains in weaning foods towards the growth of enteropathogenic E. coli and S. typhimurium was solely due to low pH and the production of volatile acid components by the organism.
    MeSH terms: Agar; Anti-Infective Agents; Bifidobacterium; Breast Feeding; Chromatography, High Pressure Liquid; Feces; Fermentation; Gelatin; Humans; Indoles; Infant; Malaysia; Peptones; Salmonella typhimurium; Weaning; Lactic Acid; Probiotics; Enteropathogenic Escherichia coli
  2. Guatelli-Steinberg D, Skinner M
    Folia Primatol., 2000 May-Jun;71(3):115-32.
    PMID: 10828689
    Ninety-seven specimens of sympatric monkeys and apes from East Malaysia and 115 monkeys and apes from West Africa are examined in order to evaluate the magnitude and nature of the great ape-monkey linear enamel hypoplasia (LEH) 'dichotomy'. This study demonstrates that great apes from both regions have a higher incidence of LEH and repetitive LEH than do gibbons and monkeys. However, the authors find that the dichotomy is not as clear-cut as previous research suggests, since some monkey samples exhibit high LEH frequencies. The authors evaluate the potential influence of great ape-monkey differences in crown height on this dichotomy. They show that canine crown height variation is weakly associated with LEH variation. Differences between monkeys and great apes in their crown formation spans and in their experience of environmental stress may be more likely causes of the dichotomy.
    MeSH terms: Africa/epidemiology; Animals; Haplorhini; Asia/epidemiology; Dental Enamel Hypoplasia/epidemiology; Dental Enamel Hypoplasia/veterinary*; Dogs; Hominidae; Prevalence; Primate Diseases/epidemiology*
  3. Ng LF, Shumacher A, Goh CB
    Palliat Med, 2000 Mar;14(2):163-4.
    PMID: 10829153 DOI: 10.1191/026921600669696020
    MeSH terms: Attitude; Cultural Characteristics; Decision Making*; Family Characteristics; Humans; Neoplasms/therapy*; Palliative Care/psychology*; Prospective Studies; Third-Party Consent
  4. du Preez LH, Lim LH
    Folia Parasitol., 2000;47(1):11-6.
    PMID: 10833010
    Neopolystoma liewi sp. n. is described from the conjunctival cavity of the Malayan box turtle Cuora amboinensis (Daudin, 1802), in Peninsular Malaysia. This is the first record of Neopolystoma in Malaysia and the fourth polystomatid species described from C. amboinensis. Of the 27 Malayan box turtles examined, 8 were found to be infected. A maximum of 2 parasites per eye and 4 individuals per host was recorded. N. liewi sp. n. differs from all other members of the genus by possessing few and short genital spines and small marginal hooks. The oncomiracidium has 64 ciliated cells arranged symmetrically about the sagittal axis.
    MeSH terms: Animals; Eye/parasitology*; Malaysia; Trematoda/anatomy & histology; Trematoda/isolation & purification*; Turtles/parasitology*
  5. Kaushik SP, Vu C
    Aust N Z J Med, 2000 Apr;30(2):231-5.
    PMID: 10833116
    BACKGROUND: From European and North American data, it is recommended in the Asia Pacific consensus statement, that one week therapy with a proton pump inhibitor, amoxycillin and clarithromycin be used for Helicobacter pylori eradication, in areas of high metronidazole resistance. The efficacy of this regimen is unknown in Singapore.

    AIM: To assess the efficacy, safety and compliance of an H. pylori eradication regimen and examine clinical factors that potentially determine eradication.

    METHODS: Consecutive outpatients from a multicultural, south east Asian, population with H. pylori infection, with or without peptic ulcer, were treated with lansoprazole 30 mg, amoxycillin 1 gm, clarithromycin 500 mg, twice a day for seven days. Eradication was assessed by either rapid urease, histology or urea breath test. Compliance and side effects were recorded. The eradication rate and effect of ethnicity, age, sex, usage of alcohol, smoking and non-steroidal anti-inflammatory drugs, history of ulcer and endoscopic diagnosis on eradication were examined by univariate and multivariate analysis.

    RESULTS: Of 113 patients, the eradication rate by intention to treat was 98/113 (87%) (95% confidence interval [CI] 80-93%) and per protocol was 98/106 (92%) (95% CI 87-97%). Using Fisher's exact test, eradication was more successful in Chinese (intention to treat and per protocol respectively p=0.02 and p<0.001) compared to non-Chinese. By logistic regression analysis ethnicity was an independent factor associated with eradication success (p=0.0025). Side effects occurred in five (4.4%), resulting in cessation of treatment.

    CONCLUSIONS: This one week eradication regimen is safe and effective in south east Asians. Chinese ethnicity may be associated with a higher likelihood of eradication success.

    MeSH terms: Amoxicillin/therapeutic use*; Anti-Ulcer Agents/therapeutic use*; China/ethnology; Ethnic Groups/statistics & numerical data; Female; Humans; India/ethnology; Malaysia/ethnology; Male; Middle Aged; Omeprazole/analogs & derivatives*; Omeprazole/therapeutic use; Prospective Studies; Singapore/epidemiology; Likelihood Functions; Logistic Models; Helicobacter pylori*; Helicobacter Infections/drug therapy*; Helicobacter Infections/ethnology; Clarithromycin/therapeutic use*; 2-Pyridinylmethylsulfinylbenzimidazoles; Lansoprazole
  6. Jayaram G, Cheah PL, Yip CH
    Acta Cytol., 2000 May-Jun;44(3):375-9.
    PMID: 10833994
    BACKGROUND: Teratoma of the thyroid in adults is extremely rare, and most are malignant. Only nine cases have been adequately documented in the English-language literature, and there are no reports detailing the fine needle aspiration (FNA) cytologic characteristics.

    CASE: A 32-year-old female presented with a left-sided nodular thyroid mass with left cervical lymphadenopathy. FNA cytology of the thyroid and lymph nodes was done. The cytologic and immunocytochemical features were that of a small round cell tumor with neuroepithelial (NE) differentiation, metastasizing to the cervical nodes. Microscopic study of the thyroidectomy specimen showed a tumor showing an NE pattern with occasional islands of squamous and cuboidal epithelium, leading to a diagnosis of malignant teratoma.

    CONCLUSION: Knowledge of FNA cytologic features of rare but highly malignant lesions like thyroid teratomas allow early recognition so that suitable and possibly aggressive treatment protocols can be adopted in the hope of prolonging survival.
    MeSH terms: Adult; Biopsy, Needle; Female; Humans; Immunohistochemistry; Lymphatic Metastasis; Teratoma/pathology*; Thyroid Neoplasms/pathology*
  7. Al-Haddawi MH, Jasni S, Zamri-Saad M, Mutalib AR, Son R, Sheikh-Omar AR
    Vet Res Commun, 2000 Apr;24(3):153-67.
    PMID: 10836274
    Sixteen 8- to 9-week-old Pasteurella multocida-free rabbits were divided into two equal groups. Eight rabbits in one group were inoculated intranasally with P. multoida type A:3. The other eight were inoculated intranasally with phosphate-buffered saline and used as controls. Nasal swabs taken before and after inoculation were cultured for bacterial isolation. Post-mortem nasal swabs and lung samples were cultured for bacteriological isolation. Nasal mucosa and lung samples were collected and processed for transmission electron microscopy. Pasteurella multocida was isolated from the nasal cavity of all infected rabbits and from the lungs of four infected rabbits. Degenerative ultrastructural changes in epithelial cells and endothelial cells were seen in the infected rabbits. Deciliation of the ciliated epithelium and hyperplasia of the goblet cells in the nasal mucosa were noted. Thickening of the alveolar septa due to hyperplasia of type II pneumocytes, swelling of the endothelial lining of capillaries and infiltration of inflammatory cells were also observed. Intracellular invasion of the nasal epithelial cells and of type II pneumocytes by the organism was observed. Coccobacilli were observed in membrane-bound vacuoles in the cytoplasm of these cells. The vacuoles were adjacent to the host-cell mitochondria and some of these vacuoles appeared to be fused to the mitochondrial membrane. Some type I pneumocytes with intracellular membrane-bound vacuoles containing bacterial cells showed protrusions, which appeared to detach into the alveolar lumina. These results indicated that P. multocida serotype A:3 in rabbits can invade the epithelial cell and cause structural changes in the interstitium, epithelium and endothelium. Heterophils and macrophages appear to play important roles in tissue injury.
    MeSH terms: Animals; Lung/microbiology*; Lung/ultrastructure; Microscopy, Electron/veterinary; Nasal Mucosa/microbiology*; Nasal Mucosa/ultrastructure; Pasteurella Infections/pathology; Pasteurella Infections/veterinary*; Rabbits*; Pasteurella multocida/isolation & purification*
  8. Rasool AH, Rahman AR, Ismail R, Hatim S, Abdullah AR, Singh R, et al.
    Int J Clin Pharmacol Ther, 2000 May;38(5):260-9.
    PMID: 10839470
    OBJECTIVE: To determine whether racial differences in response to blockade of beta receptors occur among racial groups in Malaysia that are the Malays, Indians and Chinese. SUBJECTS, MATERIALS AND METHOD: 35 healthy male volunteers representing the 3 main racial groups in Malaysia (12 Malays, 12 Chinese and 11 Indians) were studied in a randomized, placebo-controlled, crossover and single-blind design. Propranolol 80 mg 12-hourly was given orally for 48 hours. Six hours after the last dose subjects attended an exercise session where resting and exercise heart rate, blood pressure, plasma potassium and glucose levels, resting FEV1 and plasma propranolol concentrations were recorded.

    RESULTS: No significant difference in plasma propranolol (mean +/- SEM) levels was seen between races six hours after the last dose (Malays, 59.7 +/- 8.8 ng/ml, Indians, 67.6 +/- 19.3 ng/ml, Chinese, 58.4 +/- 7.9 ng/ml). Chinese were least sensitive to the bradycardic and hypotensive effects of propranolol at rest and exercise. Indians and Malays had significant reduction of supine systolic blood pressure with propranolol but not Chinese. Comparison of percentage reductions of systolic blood pressure at supine, sitting and exercise by repeated measure analysis showed the Malays to have significantly higher change compared to the Chinese (p = 0.022). Similarly, comparison of percentage reductions of heart rate at supine, sitting and exercise by repeated measure analysis showed the Malays to have significantly higher change compared to the Chinese (p = 0.040). Average change in potassium concentrations at peak exercise and recovery showed the Indians to have significantly higher increase in potassium levels with propranolol compared to the Malays (p = 0.038). However, no significant interethnic difference was seen in the reduction of glucose levels at rest, peak exercise or recovery. Also, no significant interethnic difference was seen in reduction of FEV1 values.

    CONCLUSION: We, therefore, conclude that ethnic differences in response to blockade of beta-receptors exist among racial groups in Malaysia. These differences were seen at similar plasma drug levels between races suggesting ethnic differences in drug sensitivity, rather than differences in drug disposition.

    MeSH terms: Adrenergic beta-Antagonists/pharmacokinetics; Adrenergic beta-Antagonists/pharmacology*; Adult; Blood Glucose/metabolism; Forced Expiratory Volume/physiology; Humans; Malaysia/ethnology; Male; Posture/physiology; Propranolol/pharmacokinetics; Propranolol/pharmacology*; Receptors, Adrenergic, beta/drug effects*; Receptors, Adrenergic, beta/genetics*; Continental Population Groups
  9. Razali SM
    Trop Doct, 2000 Apr;30(2):105-6.
    PMID: 10842562 DOI: 10.1177/004947550003000218
    MeSH terms: Aftercare/organization & administration; Counseling/organization & administration*; Follow-Up Studies; Humans; Islam/psychology*; Malaysia/epidemiology; Medicine, Traditional*; Organizational Innovation; Psychotherapy, Group; Relaxation Therapy; Religion and Medicine*; Program Evaluation; Substance Abuse Treatment Centers/organization & administration*; Treatment Outcome; Models, Organizational; Substance-Related Disorders/ethnology; Substance-Related Disorders/rehabilitation*
  10. Tan HM
    Int. J. Androl., 2000;23 Suppl 2:87-8.
    PMID: 10849506
    The quest for improving and maintaining sexual function has been going on since time immemorial. The advent of an effective oral drug, sildenafil, has brought about unprecedented open discussion on male erectile dysfunction, and gas accelerated the pace of development of new therapies for erectile dysfunction. New knowledge in the physiology of sexual function has enabled researchers to target drug treatment at the whole network of the central nervous system and the numerous cascadic enzymatic reactions leading to relaxation of the corporal smooth muscle. One of the brightest potential applications of future molecular technology in the study of erectile dysfuction is in the utilization of gene therapy.
    MeSH terms: Sildenafil Citrate; Humans; Erectile Dysfunction/drug therapy*; Erectile Dysfunction/physiopathology; Male; Muscle Relaxation/drug effects; Muscle, Smooth, Vascular/drug effects; Muscle, Smooth, Vascular/physiopathology; Penis/blood supply; Phosphodiesterase Inhibitors/pharmacology; Phosphodiesterase Inhibitors/therapeutic use; Piperazines/pharmacology; Piperazines/therapeutic use*; Purines; Sulfones
  11. Julianto T, Yuen KH, Noor AM
    Int J Pharm, 2000 Apr 25;200(1):53-7.
    PMID: 10845685
    A single dose study was conducted to evaluate the bioavailability of a novel self-emulsifying vitamin E preparation, in comparison with that of a commercial product, Natopherol, available as soft gelatin capsules under fasted condition. The self-emulsifying preparation achieved a faster rate and higher extent of absorption. A statistically significant difference was observed between the values of the two preparations in the parameters AUC, Cmax and Tmax. Moreover, the 90% confidence interval of the logarithmic transformed AUC values of the self-emulsifying preparation over those of the soft gelatin capsule product was found to be between 2.1 and 4.1, suggesting an increase in bioavailability of between 210 and 410%. As for Cmax, the 90% confidence interval was between 2.1 and 3.0. However, no statistically significant difference was observed between the t(1/2) values estimated from the plasma concentration versus time data of the two preparations. The values are also comparable to those reported in the literature.
    MeSH terms: Adult; Biological Availability; Capsules; Chemistry, Pharmaceutical; Emulsions; Gelatin; Humans; Male; Vitamin E/administration & dosage*; Vitamin E/blood; Vitamin E/pharmacokinetics*; Cross-Over Studies; Area Under Curve
  12. Harrison RD
    Proc Biol Sci, 2000 May 7;267(1446):911-5.
    PMID: 10853734
    Figs (Ficus spp.) and their species-specific pollinators, the fig wasps (Agaonidae), have coevolved one of the most intricate interactions found in nature, in which the fig wasps, in return for pollination services, raise their offspring in the fig inflorescence. Fig wasps, however, have very short adult lives and hence are dependent on the near-continuous production of inflorescences to maintain their populations. From January to March 1998 northern Borneo suffered a very severe drought linked to the El Niño-Southern Oscillation event of 1997-1998. This caused a substantial break in the production of inflorescences on dioecious figs and led to the local extinction of their pollinators at Lambir Hills National Park, Sarawak, Malaysia. Most pollinators had not recolonized six months after the drought and, given the high level of endemism and wide extent of the drought, some species may be totally extinct. Cascading effects on vertebrate seed dispersers, for which figs are often regarded as keystone resources, and the tree species dependent on their services are also likely. This has considerable implications for the maintenance of biodiversity under a scenario of climate change and greater climatic extremes.
    MeSH terms: Animals; Borneo; Climate*; Female; Male; Symbiosis*; Trees; Wasps; Ecosystem*
  13. Nair S, Schreiber E, Thong KL, Pang T, Altwegg M
    J Microbiol Methods, 2000 Jun;41(1):35-43.
    PMID: 10856775
    Amplified fragment length polymorphism (AFLP) is a recently developed, PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In the present study, AFLP was evaluated for its usefulness in the molecular typing of Salmonella typhi in comparison to ribotyping and pulsed-field gel electrophoresis (PFGE). Six S. typhi isolates from diverse geographic areas (Malaysia, Indonesia, India, Chile, Papua New Guinea and Switzerland) gave unique, heterogeneous profiles when typed by AFLP, a result which was consistent with ribotyping and PFGE analysis. In a further study of selected S. typhi isolates from Papua New Guinea which caused fatal and non-fatal disease previously shown to be clonally related by PFGE, AFLP discriminated between these isolates but did not indicate a linkage between genotype with virulence. We conclude that AFLP (discriminatory index=0.88) has a higher discriminatory power for strain differentiation among S. typhi than ribotyping (DI=0.63) and PFGE (DI=0.74).
    MeSH terms: DNA, Bacterial/analysis; DNA, Ribosomal/analysis; Genotype; Humans; Polymorphism, Restriction Fragment Length; Salmonella typhi/classification*; Salmonella typhi/genetics; Polymerase Chain Reaction; DNA Fingerprinting/methods*; Electrophoresis, Gel, Pulsed-Field
  14. Harcourt BH, Tamin A, Ksiazek TG, Rollin PE, Anderson LJ, Bellini WJ, et al.
    Virology, 2000 Jun 5;271(2):334-49.
    PMID: 10860887
    Recently, a new paramyxovirus, now known as Nipah virus (NV), emerged in Malaysia and Singapore, causing fatal encephalitis in humans and a respiratory syndrome in pigs. Initial studies had indicated that NV is antigenically and genetically related to Hendra virus (HV). We generated the sequences of the N, P/C/V, M, F, and G genes of NV and compared these sequences with those of HV and other members of the family Paramyxoviridae. The intergenic regions of NV were identical to those of HV, and the gene start and stop sequences of NV were nearly identical to those of HV. The open reading frames (ORFs) for the V and C proteins within the P gene were found in NV, but the ORF encoding a potential short basic protein found in the P gene of HV was not conserved in NV. The N, P, C, V, M, F, and G ORFs in NV have nucleotide homologies ranging from 88% to 70% and predicted amino acid homologies ranging from 92% to 67% in comparison with HV. The predicted fusion cleavage sequence of the F protein of NV had a single amino acid substitution (K to R) in comparison with HV. Phylogenetic analysis demonstrated that although HV and NV are closely related, they are clearly distinct from any of the established genera within the Paramyxoviridae and should be considered a new genus.
    MeSH terms: Amino Acid Sequence; Animals; Cercopithecus aethiops; Cloning, Molecular; Glycoproteins/genetics; Molecular Sequence Data; Nucleoproteins/genetics; Phosphoproteins/genetics; Phylogeny; Transcription, Genetic; Vero Cells; Viral Fusion Proteins/genetics; Viral Matrix Proteins/genetics; Viral Proteins/genetics; Sequence Homology, Amino Acid; Sequence Analysis, DNA; Paramyxovirinae/classification; Paramyxovirinae/genetics*; Mice
  15. Billa N, Yuen KH, Khader MA, Omar A
    Int J Pharm, 2000 May 15;201(1):109-20.
    PMID: 10867269
    A xanthan gum matrix controlled release tablet formulation containing diclofenac sodium was evaluated in vitro and was found to release the drug at a uniform rate. The gastrointestinal transit behaviour of the formulation as determined by gamma scintigraphy, using healthy male volunteers under fasted and fed conditions, indicated that gastric emptying was delayed with food intake. In contrast, the small intestinal transit remained practically unchanged under both food statuses. Therefore, the delay in caecal arrival observed in the fed state can be attributed to the delay in gastric emptying. Rate of diclofenac sodium absorption was generally higher in the fed state compared to the fasted state, however the total amount absorbed under both food statuses remained practically the same. The rate of in vivo dissolution of the drug in the fed state was faster compared to that in the fasted state. Thus, at the time of caecal arrival, in vivo dissolution was complete in the fed state, unlike in the fasted state, where almost 60% of the drug was delivered to the colon.
    MeSH terms: Adult; Anti-Inflammatory Agents, Non-Steroidal/administration & dosage; Anti-Inflammatory Agents, Non-Steroidal/blood; Anti-Inflammatory Agents, Non-Steroidal/pharmacokinetics*; Biological Availability; Delayed-Action Preparations; Diclofenac/administration & dosage; Diclofenac/blood; Diclofenac/pharmacokinetics*; Eating; Excipients; Gastrointestinal Transit/physiology*; Humans; Male; Polysaccharides, Bacterial; Solubility; Tablets; Technetium; Area Under Curve
  16. Cheah YC, Parker G, Roy K
    Soc Psychiatry Psychiatr Epidemiol, 2000 Apr;35(4):170-6.
    PMID: 10868082
    The Profile of Community Psychiatry Clients (PCPC) was developed in a Sydney-based sample of those with a mental illness as a 35-item measure of likely need for service recognition, review and possible assistance.
    MeSH terms: Adult; Mental Disorders/epidemiology; Mental Disorders/therapy*; Catchment Area (Health); Community Mental Health Services/standards*; Community Mental Health Services/supply & distribution*; Female; Humans; Malaysia/epidemiology; Male; Reproducibility of Results; Needs Assessment*
  17. Chan YK, Ng KP
    J Obstet Gynaecol Res, 2000 Apr;26(2):137-40.
    PMID: 10870307
    OBJECTIVE: A survey covering 30% of the deliveries in Malaysia was done to determine the practice of obstetric anaesthesia and analgesia for 1996.

    RESULTS: From the survey, it was found that the regional anaesthesia rate for caesarean section was 46% in the government hospitals compared to 29.2% in the private hospitals, with spinal anaesthesia being the most common regional anaesthetic technique used in both types of hospitals. The epidural rate for labour analgesia was only 1.5% overall for the country. Epidural analgesia services were available in all private hospitals whereas 17.6% of government hospitals surveyed did not offer this service at all.

    CONCLUSIONS: Although the use of epidural analgesia for labour was low in Malaysia, the overall rate of regional anaesthesia for caesarean section (41.9%) is very much in keeping with the standards of safe practice recommended by the United Kingdom.

    MeSH terms: Anesthesia, Obstetrical/statistics & numerical data*; Female; Humans; Malaysia/epidemiology; Practice Patterns, Physicians'*; Pregnancy; Surveys and Questionnaires; Analgesia, Obstetrical/statistics & numerical data*
  18. Nadesan K
    Am J Forensic Med Pathol, 2000 Jun;21(2):107-13.
    PMID: 10871122
    True vehicular homicides are defined as those occurrences in which a motor vehicle is intentionally used as a weapon in taking of a life. A case is presented in which the deceased was traveling in the front passenger seat of a motor car that was deliberately rammed by a heavy jeep that came in the opposite direction, resulting in a serious frontal collision. Immediately after the impact, while the occupants of the car were lying in a dazed condition, the two persons riding in the jeep escaped with a bag containing money that was in the car, leaving the jeep behind. The impact mainly involved the driver's sides of both vehicles. The driver of the car sustained serious injuries but was found to be alive, whereas the front-seat passenger, who did not show any serious external injuries, was found to be in a collapsed state and was pronounced dead on admission to the hospital within 30 minutes of the accident. The autopsy revealed that death was caused by closed hemopericardium from a ruptured right atrium. The evaluation of the external and internal injuries confirmed that the fatal injury and a few serious internal injuries were caused by the seat belt (tertiary-impact injuries). The ruptured right atrium was attributed to blunt abdominal trauma by impacting against the lap belt. The case was a true vehicular homicide in which a motor vehicle had been used as a weapon to kill a person. Various aspects pertaining to road accidents, the safety of the occupants, and the advantage and disadvantage of the safety devices are discussed.
    MeSH terms: Accidents, Traffic/legislation & jurisprudence*; Autopsy; Forensic Medicine; Heart Atria/injuries; Homicide*; Humans; Malaysia; Male; Middle Aged; Multiple Trauma/pathology*; Pericardial Effusion/pathology; Rupture; Fatal Outcome
  19. Peh KK, Wong CF
    Drug Dev Ind Pharm, 2000 Jul;26(7):723-30.
    PMID: 10872090
    Controlled-release grade hydroxypropylmethylcellulose (HPMC) or xanthan gum (XG) and microcrystalline cellulose (MCC) were employed to prepare controlled-release diltiazem hydrochloride tablets. The similarity factor f2 was used for dissolution profile comparison using Herbesser 90 SR as a reference product. Drug release could be sustained in a predictable manner by modifying the content of HPMC or XG. Moreover, the drug release profiles of tablets prepared using these matrix materials were not affected by pH and agitation rate. The f2 values showed that only one batch of tablets (of diltiazem HCl, HPMC or XG, and MCC in proportions of 3.0:3.0:4.0) was considered similar to that of the reference product, with values above 50. The unbiased similarity factor f2* values were not much different from the f2 values, ascribing to a small dissolution variance of the test and reference products. The amount of HPMC or XG incorporated to produce tablets with the desired dissolution profile could be determined from the curves of f2 versus polymer content. Hence, the f2 values can be applied as screening and optimization tools during development of controlled-release preparations.
    MeSH terms: Antihypertensive Agents/administration & dosage*; Chemistry, Pharmaceutical/methods*; Delayed-Action Preparations; Diltiazem/administration & dosage*; Drug Carriers/chemistry; Hydrogen-Ion Concentration; Lactose/analogs & derivatives; Lactose/chemistry; Methylcellulose/analogs & derivatives; Methylcellulose/chemistry; Oxazines; Polysaccharides, Bacterial/chemistry; Tablets
  20. Yuen KH, Wong JW, Peh KK, Julianto T, Choy WP
    Drug Dev Ind Pharm, 2000 Jul;26(7):803-7.
    PMID: 10872103
    The bioavailability of a generic preparation of pentoxifylline sustained-release (SR) tablet was evaluated in comparison with a proprietary product (Trental 400). For the study, 12 healthy male volunteers participated; the study was conducted according to a randomized, two-way crossover design. The bioavailability was compared using the parameters total area under the plasma level-time curve AUC0-infinity, peak plasma concentration Cmax, and time to reach peak plasma concentration Tmax. No statistically significant difference was observed between the values of the two products in all three parameters. The 90% confidence interval for the ratio of the logarithmic transformed AUC0-infinity values of the generic pentoxifylline over those of Trental 400 was found to lie between 0.83 and 1.00, while that of the parameter Cmax was between 0.91 and 1.29. In addition, elimination half-life t1/2 and apparent volume of distribution Vd were calculated. There was no statistically significant difference between the t1/2 Vd values obtained from the data of the two preparations.
    MeSH terms: Administration, Oral; Adult; Analysis of Variance; Biological Availability; Chromatography, High Pressure Liquid; Delayed-Action Preparations; Half-Life; Hematologic Agents/administration & dosage; Hematologic Agents/blood; Hematologic Agents/pharmacokinetics*; Humans; Male; Middle Aged; Pentoxifylline/administration & dosage; Pentoxifylline/blood; Pentoxifylline/pharmacokinetics*; Cross-Over Studies; Area Under Curve
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