Browse publications by year: 2000

  1. Yeang HY
    Ann. Allergy Asthma Immunol., 2000 Jun;84(6):628-32.
    PMID: 10875493 DOI: 10.1016/S1081-1206(10)62415-5
    BACKGROUND:
    The prevalence of latex-specific IgE computed from the results of serologic assays is commonly thought to reflect, to a greater or lesser extent, the prevalence of latex allergy and its implied risk.

    OBJECTIVE:
    The study examines how imperfect test specificity of in vitro assays influences the precision of latex allergy prevalence that it estimates.

    METHODS:
    Various models encompassing a range of hypothetical test sensitivity and specificity values are investigated to gauge their influence on the estimate of latex allergy prevalence. The models examine these interactions in situations of high or low allergy prevalence.

    RESULTS:
    Serologic latex diagnostic assays with test specificity within the range of those of commercially available assays can greatly overestimate prevalence where the true prevalence is low (eg, of the order of one in 100 or one in 1,000). A formula to correct for errors in prevalence estimates arising from imperfect test sensitivity and specificity of an in vitro assay is presented.

    CONCLUSION:
    While serologic assays for latex IgE pose few hazards to the patient and are useful for confirming the diagnosis of latex allergy, the test results may vastly overestimate the true prevalence of latex allergy and its associated risks in situations where latex allergy is actually rare.
    MeSH terms: Diagnostic Errors; False Negative Reactions; False Positive Reactions; Humans; Methods; Sensitivity and Specificity; Reproducibility of Results; Prevalence; Latex Hypersensitivity/diagnosis; Latex Hypersensitivity/epidemiology*
  2. Ismail A
    Malays J Med Sci, 2000 Jul;7(2):3-8.
    PMID: 22977383 MyJurnal
    For effective management of typhoid, diagnosis of the disease must be done with speed and accuracy. Development of such a test would require antigens that are specific for typhoid diagnosis. Attempts at finding the specific antigen have been carried out throughout the years. The finding of such an antigen can lead to carrier detection as well. Candidate antigens have been used in the development of antigen or antibody detection tests with variation in sensitivity and specificity. Further characterization and understanding of the candidate antigens combined with use of innovative technologies will allow for the ideal test for typhoid and typhoid carriers to be within reach.
    MeSH terms: Carrier State; Immunologic Tests; Salmonella typhi; Sensitivity and Specificity; Typhoid Fever
  3. Rahim AF, Hitam WH, Alwi MN, Ghazali G, Rahim SZ
    Malays J Med Sci, 2000 Jul;7(2):9-12.
    PMID: 22977384
    To aid future curriculum revision and planning, a batch of newly graduated medical students were surveyed using a questionnaire containing items representing possible areas of concern during house-officership. Students rated items representing communication issues as areas of concern. They did not agree that areas concerning responsibilities as a doctor, continuing medical education, theoretical and practical skills and potentially stressful working conditions were problem areas. Communication skills should remain among the priority areas for undergraduate training. Students should also be given more information about the house-officership period prior to graduation. Further study is needed to confirm perceived strengths of the USM curriculum suggested by the study, which are skills in finding resources for further learning and skills in leadership. A task-analysis of the house-officership period is also needed.
    MeSH terms: Curriculum; Education, Medical, Continuing; Leadership; Physicians; Surveys and Questionnaires; Students, Medical
  4. Musa R, Kyi W, Rampal KG
    Malays J Med Sci, 2000 Jul;7(2):13-7.
    PMID: 22977385 MyJurnal
    A cross sectional study was carried out to evaluate the extent of occupational health problems focusing on some aspects of musculoskeletal symptoms among batik workers in Kelantan, Malaysia. The workers selected must have been in that industry for at least one year. Using cluster sampling, 202 workers were selected from 21 factories. More than half (60.2%) of the workers had been troubled with musculoskeletal symptoms at work. The most common symptoms were pain over the shoulders (41.0%), lower back (34.4%) and ankle (34.4%). Duration of employment, younger age group, prolonged standing and awkward working task were among contributing factors. It is therefore necessary to improve on both ergonomic and psychosocial environments of batik workers in order to prevent these musculoskeletal symptoms.
    MeSH terms: Adult; Cross-Sectional Studies; Humans; Malaysia; Musculoskeletal Pain*
  5. Abd-Manan F
    Malays J Med Sci, 2000 Jul;7(2):18-26.
    PMID: 22977386 MyJurnal
    Previous studies have shown that stress on the vergence and accommodation systems, either artificially induced or naturally occurring, results in small misalignment of the visual axes, reduces binocular visual acuity and produces symptoms of ocular discomfort. This study examines the effect of artificially induced visual stress using ophthalmic prisms on three dimensional perception on 30 optometry students ages ranging from 19 to 29 years old. 6D base-in prisms, equally divided between the eyes (3D base-in each) was used to induce stress on the visual system producing misalignment of visual axes known as fixation disparity. The fixation disparity is quantified using near vision Mallett Unit and an enlarged scaled diagram. Stereoscopic perception was measured with the TNO test, with and without the presence of stress and the results was compared. Wilcoxon's matched pair ranked tests show statistically significant difference in the stereo thresholds of both conditions, p = 0.01 for advancing stereopsis and p = 0.01 for receding stereopsis, respectively. The study concludes that visual stress induced by prisms, produce misalignment of the visual axes and thus reduces three dimensional performance.
    MeSH terms: Accommodation, Ocular; Depth Perception; Eye; Myopia; Optometry; Students; Visual Acuity; Vision Disparity
  6. Raman R
    PMID: 23119711 DOI: 10.1007/BF03006219
    A micro anatomical feature in the ear useful in surgery is described.
    MeSH terms: Ear, Middle; Temporal Bone
  7. Bidawid S, Farber JM, Sattar SA
    Appl Environ Microbiol, 2000 Jul;66(7):2759-63.
    PMID: 10877765
    Hepatitis A virus (HAV) is an important pathogen which has been responsible for many food-borne outbreaks. HAV-excreting food handlers, especially those with poor hygienic practices, can contaminate the foods which they handle. Consumption of such foods without further processing has been known to result in cases of infectious hepatitis. Since quantitative data on virus transfer during contact of hands with foods is not available, we investigated the transfer of HAV from artificially contaminated fingerpads of adult volunteers to pieces of fresh lettuce. Touching the lettuce with artificially contaminated fingerpads for 10 s at a pressure of 0.2 to 0.4 kg/cm(2) resulted in transfer of 9.2% +/- 0.9% of the infectious virus. The pretreatments tested to interrupt virus transfer from contaminated fingerpads included (i) hard-water rinsing and towel drying, (ii) application of a domestic or commercial topical agent followed by water rinsing and towel drying, and (iii) exposure to a hand gel containing 62% ethanol or 75% liquid ethanol without water rinsing or towel drying. When the fingerpads were treated with the topical agents or alcohol before the lettuce was touched, the amount of infectious virus transferred to lettuce was reduced from 9.2% to between 0.3 and 0.6% (depending on the topical agent used), which was a reduction in virus transfer of up to 30-fold. Surprisingly, no virus transfer to lettuce was detected when the fingerpads were rinsed with water alone before the lettuce was touched. However, additional experiments with water rinsing in which smaller volumes of water were used (1 ml instead of 15 ml) showed that the rate of virus transfer to lettuce was 0.3% +/- 0.1%. The variability in virus transfer rates following water rinsing may indicate that the volume of water at least in part influences virus removal from the fingerpads differently, a possibility which should be investigated further. This study provided novel information concerning the rate of virus transfer to foods and a model for investigating the transfer of viral and other food-borne pathogens from contaminated hands to foods, as well as techniques for interrupting such transfer to improve food safety.
    MeSH terms: Adult; Disinfection/methods; Female; Fingers/virology; Food Contamination/prevention & control*; Food Handling*; Food Microbiology*; Hand Disinfection; Hepatitis A/transmission*; Hepatitis A/virology; Hepatovirus/isolation & purification*; Hepatovirus/physiology; Humans; Male; Middle Aged; Viral Plaque Assay; Lettuce/virology
  8. Menon BS, Reddy SC, Maziah WM, Ham A, Rosline H
    Med. Pediatr. Oncol., 2000 Jul;35(1):75-6.
    PMID: 10881012
    MeSH terms: Child, Preschool; Female; Humans; Infant; Malaysia/epidemiology; Male; Medical Records; Retinoblastoma/epidemiology*; Retinoblastoma/secondary*; Retrospective Studies; Retinal Neoplasms/epidemiology*; Retinal Neoplasms/pathology*
  9. Bhattachary-Chatterjee M, Nath Baral R, Chatterjee SK, Das R, Zeytin H, Chakraborty M, et al.
    Cancer Immunol Immunother, 2000 Jun;49(3):133-41.
    PMID: 10881692
    Anti-idiotype (Id) vaccine therapy has been tested and shown to be effective, in several animal models, for triggering the immune system to induce specific and protective immunity against bacterial, viral and parasitic infections. The administration of anti-Id antibodies as surrogate tumor-associated antigens (TAA) also represents another potential application of the concept of the Id network. Limited experience in human trials using anti-Id to stimulate immunity against tumors has shown promising results. In this "counter-point" article, we discuss our own findings showing the potential of anti-Id antibody vaccines to be novel therapeutic approaches to various human cancers and also discuss where anti-Id vaccines may perform better than traditional multiple-epitope antigen vaccines.
    MeSH terms: Adjuvants, Immunologic; Alum Compounds; Animals; Antibodies, Anti-Idiotypic/adverse effects; Antibodies, Anti-Idiotypic/immunology*; Antibodies, Anti-Idiotypic/therapeutic use; Antibodies, Monoclonal/adverse effects; Antibodies, Monoclonal/immunology*; Antibodies, Monoclonal/therapeutic use; Antibodies, Neoplasm/biosynthesis; Antibodies, Neoplasm/immunology; Epitopes/immunology*; Antigens, Neoplasm/immunology*; Breast Neoplasms/therapy; Carcinoembryonic Antigen/immunology; Clinical Trials as Topic; Evaluation Studies as Topic; Gangliosides/immunology; Glycolipids/immunology; Glycoproteins/immunology; Humans; Immunization; Melanoma/therapy; Mice, Inbred C57BL; Neoplasms/prevention & control; Neoplasms/therapy*; Neoplasms, Experimental/therapy; Colorectal Neoplasms/therapy; Leukemia-Lymphoma, Adult T-Cell/immunology; Treatment Outcome; Models, Immunological; Molecular Mimicry*; Cancer Vaccines/adverse effects; Cancer Vaccines/immunology*; Cancer Vaccines/therapeutic use; Mice; Lipid Droplets
  10. Yazid AM, Ali AM, Shuhaimi M, Kalaivaani V, Rokiah MY, Reezal A
    Lett Appl Microbiol, 2000 Jul;31(1):57-62.
    PMID: 10886616
    Eighteen Bifidobacterium strains were tested for their susceptibility to a range of antimicrobial agents. All the strains tested, including the reference culture Lactobacillus acidophilus CH2, were susceptible to several groups of antimicrobial agents, they were cephalosporin (cefamandole, cefazolin, cefaperazone, cefoxitin), polypeptide (bacitracin), macrolide (erythromycin), penicillin (amoxicillin), phenicol (chloramphenicol) and beta-lactam (imipenem). Fourteen strains were resistant to more than 10 antibiotics. The reference culture was resistant to only three antibiotics. The results showed that bifidobacteria are resistant to a wide range of antimicrobial agents.
    MeSH terms: Anti-Bacterial Agents/pharmacology*; Bifidobacterium/drug effects*; Drug Resistance, Microbial*; Lactobacillus acidophilus/drug effects; Microbial Sensitivity Tests
  11. Zakaria R, Ismail Z, Chatterjee A
    Pharmacol Res, 2000 Aug;42(2):183-6.
    PMID: 10887050
    Reproductive dysfunction in the female diabetic rat is associated with impaired hypothalamic-hypophyseal system, anovulation, insufficiency of ovarian steroidogenesis and spontaneous failure of pregnancy. Formation of decidua, the highly modified endometrium of pregnancy and pseudopregnancy could only be achieved when the uterus was sensitized by a sequence of oestrogen and progesterone. In this study, we examined whether the impaired expression of endometrial decidualization in the pseudopregnant rat is linked with diabetes-associated hypersecretion of testosterone. Rats were made pseudopregnant by sterile mating. Diabetes was induced by streptozotocin on day 1 p.c. Deciduogenic stimulus was given on day 5 p.c. Treatment of cyproterone acetate (10 mg kg(-1)) was scheduled from day 5 through day 9 p.c. Animals were killed on day 10 p.c, and the degree of endometrial decidual growth, plasma levels of oestradiol, progesterone, ACTH and testosterone were determined. Results showed that compared to controls there was a concomitant drop in endometrial decidual growth concurrently with impaired levels of oestradiol and progesterone in diabetic pseudopregnant rats. ACTH and testosterone levels were, however, profoundly elevated. Cyproterone acetate treatment in the diabetic pseudopregnant rat resulted in a simultaneous elevation of oestradiol and progesterone, which eventually helped the endometrial differentiation to decidua in the diabetic pseudopregnant rat parallel to controls. Present experimental data suggest that diabetes-associated impaired endometrial decidualization in the pseudopregnant rat is possibly caused by testosterone-induced oestrogen deficiency.
    MeSH terms: Adrenocorticotropic Hormone/blood; Androgen Antagonists/pharmacology*; Animals; Decidua/drug effects*; Decidua/growth & development; Decidua/physiopathology*; Diabetes Mellitus, Experimental/blood; Diabetes Mellitus, Experimental/physiopathology*; Estrogens/blood; Female; Organ Size/drug effects; Progesterone/blood; Pseudopregnancy/blood; Pseudopregnancy/physiopathology*; Uterus/anatomy & histology; Uterus/drug effects; Rats, Wistar; Cyproterone Acetate/pharmacology*; Rats
  12. Boo NY, Zuraidah AL, Lim NL, Zulfiqar MA
    J Trop Pediatr, 2000 Jun;46(3):172-5.
    PMID: 10893920
    A case-control study was carried out on 97 consecutive preterm (< 37 weeks) infants to determine predictors associated with failure of nasal continuous positive airway pressure (CPAP) in the treatment of respiratory distress syndrome (RDS). Logistic regression analysis showed that only three risk factors were significantly associated with failed CPAP. These were: moderate or severe RDS (odds ratio: 5.9; 95 per cent confidence interval (CI): 2.2-16.0); septicemia during CPAP therapy (OR: 8.8; 95 per cent: CI 1.5-50.7); and pneumothorax during CPAP therapy (odds ratio: 6.9; 95 per cent: CI 1.1-41.7).
    MeSH terms: Apgar Score; Humans; Infant, Newborn; Infant, Premature; Pneumothorax/etiology; Positive-Pressure Respiration*; Predictive Value of Tests; Respiratory Distress Syndrome, Newborn/complications; Respiratory Distress Syndrome, Newborn/therapy*; Risk Factors; Logistic Models; Case-Control Studies; Treatment Failure; Sepsis/etiology
  13. Awang MB, Jaafar AB, Abdullah AM, Ismail MB, Hassan MN, Abdullah R, et al.
    Respirology, 2000 Jun;5(2):183-96.
    PMID: 10894109
    OBJECTIVE: Observations have been made on the long-term trends of major air pollutants in Malaysia including nitrogen dioxide, carbon monoxide, the ozone and total suspended particulate matter (particularly PM10), and sulfur dioxide, emitted from industrial and urban areas from early 1970s until late 1998.

    METHODOLOGY: The data show that the status of atmospheric environment in Malaysia, in particular in highly industrialized areas such as Klang Valley, was determined both by local and transboundary emissions and could be described as haze and non-haze periods.

    RESULTS: During the non-haze periods, vehicular emissions accounted for more than 70% of the total emissions in the urban areas and have demonstrated two peaks in the diurnal variations of the aforementioned air pollutants, except ozone. The morning 'rush-hour' peak was mainly due to vehicle emissions, while the late evening peak was mainly attributed to meteorological conditions, particularly atmospheric stability and wind speed. Total suspended particulate matter was the main pollutant with its concentrations at few sites often exceeding the Recommended Malaysia Air Quality Guidelines. The levels of other pollutants were generally within the guidelines. Since 1980, six major haze episodes were officially reported in Malaysia: April 1983, August 1990, June 1991, October 1991, August to October 1994, and July to October 1997. The 1997 haze episode was the worst ever experienced by the country. Short-term observations using continuous monitoring systems during the haze episodes during these periods clearly showed that suspended particulate matter (PM10) was the main cause of haze and was transboundary in nature. Large forest fires in parts of Sumatra and Kalimantan during the haze period, clearly evident in satellite images, were identified as the probable key sources of the widespread heavy haze that extended across Southeast Asia from Indonesia to Singapore, Malaysia and Brunei. The results of several studies have also provided strong evidence that biomass burning is the dominating source of particulate matter. The severity and extent of 1997's haze pollution was unprecedented, affecting some 300 million people across the region. The amount of economic costs suffered by Southeast Asian countries during this environmental disaster was enormous and is yet to be fully determined. Among the important sectors severely affected were air and land transport, shipping, construction, tourism and agro-based industries. The economic cost of the haze-related damage to Malaysia presented in this study include short-term health costs, production losses, tourism-related losses and the cost of avertive action. Although the cost reported here is likely to be underestimated, they are nevertheless significant (roughly RM1 billion).

    CONCLUSIONS: The general air quality of Malaysia since 1970 has deteriorated. Studies have shown that should no effective countermeasures be introduced, the emissions of sulfur dioxide, nitrogen oxides, particulate matter, hydrocarbons and carbon monoxide in the year 2005 would increase by 1.4, 2.12, 1.47 and 2.27 times, respectively, from the 1992 levels.

    MeSH terms: Air Pollutants/analysis*; Carbon Monoxide/analysis; Fires; Humans; Lead/analysis; Malaysia/epidemiology; Nitrogen Dioxide/analysis; Ozone/analysis; Respiratory Tract Diseases/epidemiology*; Smoke/adverse effects; Sulfur Dioxide/analysis; Trees
  14. Wong CY
    Br J Fam Plann, 2000 Apr;26(2):117.
    PMID: 10896464
    MeSH terms: Contraceptive Agents, Female*; Cultural Characteristics*; Female; Humans; Intrauterine Devices, Medicated*; Islam*; Malaysia; Levonorgestrel*; Progesterone Congeners*
  15. Heng DM, Lee J, Chew SK, Tan BY, Hughes K, Chia KS
    Ann Acad Med Singap, 2000 Mar;29(2):231-6.
    PMID: 10895345
    INTRODUCTION: This is the first prospective cohort study in Singapore to describe the incidence of ischaemic heart disease (IHD) and stroke among Chinese, Malays and Asian Indians.

    MATERIALS AND METHODS: The Singapore Cardiovascular Cohort Study is a longitudinal follow-up study on a general population cohort of 5920 persons drawn from 3 previous cross-sectional surveys. Morbidity and mortality from IHD and stroke were ascertained by record linkage using a unique identification number with the death registry, Singapore Myocardial Infarct Registry and in-patient discharge databases.

    RESULTS: There were 193 first IHD events and 97 first strokes during 52,806 person-years of observation. The overall incidence of IHD was 3.8/1000 person-years and that of stroke was 1.8/1000 person-years. In both males and females, Indians had the highest IHD incidence, followed by Malays and then Chinese. For males after adjusting for age, Indians were 2.78 times (95% CI 1.86, 4.17; P < 0.0001) and 2.28 times (95% CI 1.34, 3.88; P = 0.002) more likely to get IHD than Chinese and Malays respectively. For females after adjusting for age, Indians were 1.97 times (95% CI 1.07, 3.63; P = 0.03) and 1.37 times (95% CI 0.67, 2.80; P = 0.39) more likely to get IHD than Chinese and Malays respectively. For stroke, male Chinese and Indians had higher incidence than Malays (though not statistically significant). However, in females, Malays had the highest incidence of stroke, being 2.57 times (95% CI 1.31, 5.05; P = 0.008) more likely to get stroke than Chinese after adjustment for age.

    CONCLUSIONS: This prospective study of both mortality and morbidity has confirmed the higher risk of IHD in Indians. It has also found that Malay females have a higher incidence of stroke, which deserves further study because of its potential public health importance.

    MeSH terms: Adult; Aged; China/ethnology; Cross-Sectional Studies; Female; Humans; India/ethnology; Longitudinal Studies; Malaysia/ethnology; Male; Middle Aged; Probability; Registries; Risk Factors; Singapore/epidemiology; Cohort Studies; Incidence; Survival Rate; Confidence Intervals; Myocardial Ischemia/diagnosis; Myocardial Ischemia/ethnology*; Myocardial Ischemia/mortality; Age Distribution; Sex Distribution; Stroke/diagnosis; Stroke/ethnology*; Stroke/mortality; Asian Continental Ancestry Group*
  16. Chiu CL, Ong GS
    Ann Acad Med Singap, 2000 Mar;29(2):256-8.
    PMID: 10895351
    INTRODUCTION: We report a case of subcutaneous emphysema and pneumomediastinum that presented postoperatively after tracheal extubation.

    CLINICAL PICTURE: A 51-year-old man had an uneventful anaesthesia lasting about 6.5 hours. Intubation was performed by a very junior medical officer and was considered difficult. He developed sore throat, chest pain, numbness of both hands and palpable crepitus around the neck postoperatively. Chest X-ray revealed diffuse subcutaneous emphysema, pneumomediastinum and possible pneumopericardium.

    TREATMENT: He was treated conservatively with bed rest, oxygen, analgesia, antibiotic prophylaxis, reassurance and close monitoring.

    OUTCOME: The patient made an uneventful recovery.

    CONCLUSIONS: We discussed the possible causes.

    MeSH terms: Anesthesia, General/adverse effects; Anesthesia, General/methods; Follow-Up Studies; Humans; Intubation, Intratracheal/adverse effects*; Intubation, Intratracheal/methods; Male; Mediastinal Emphysema/diagnosis; Mediastinal Emphysema/etiology*; Mediastinal Emphysema/therapy; Middle Aged; Subcutaneous Emphysema/diagnosis; Subcutaneous Emphysema/etiology*; Subcutaneous Emphysema/therapy; Arthroplasty, Replacement, Hip/adverse effects
  17. Teoh LO, Ishikawa H, Liebmann JM, Ritch R
    Arch. Ophthalmol., 2000 Jul;118(7):989-90.
    PMID: 10900117
    MeSH terms: Aged; Cell Division; Female; Humans; Intraocular Pressure; Iris/pathology*; Iris/surgery; Iris/ultrasonography; Male; Middle Aged; Pigment Epithelium of Eye/pathology*; Pigment Epithelium of Eye/ultrasonography; Recurrence; Glaucoma, Angle-Closure/etiology*; Glaucoma, Angle-Closure/surgery; Glaucoma, Angle-Closure/ultrasonography; Laser Therapy*
  18. Tabuk TC, Ulger S
    Med Parazitol (Mosk), 2000 Apr-Jun.
    PMID: 10900916
    Turkey is the last country in the temperate zone on the edge of the European continent in which malaria is prevalent at endemic and occasionally epidemic proportions. Malaria was the most significant vector borne disease constituting a serious healthy problem until it was suppressed in 1965. Following the establishment of malaria eradication program in 1957 which began operation in 1960 after many years of malaria control, the incidence of malaria decreased annually and the stricken areas became more and more restricted. Unfortunately, an agricultural development program initiated in mid 70's in the Cukurova Plain caused a substantial migration of workers from the eastern areas where malaria at that time was more prevalent. This population movement together with the industrial expansion that took place resulted in a serious epidemic of vivax malaria in 1977 in the provinces of Adana, Icel and Hatay, where 101,867 cases were reported. The following years, Turkey targeted to reduce the number of malaria cases to less than 800 by 1984. After 1985, the number of malaria cases in the country has continued to increase and in the past five and six years a serious malaria epidemics has been building up in the southeastern provinces. The gravitational center of the disease has now moved from the Cukurova to the GAP area in South East Anatolia and beyond. The indicator of this movement is that 89% of total cases in 1998 is concerning to the GAP region. By the year 1998 the number of reported cases were 36,842. The common parasite type is P. vivax in the country. The other types are generally imported from other countries. These are Syria, S. Arabia, Pakistan, Afghanistan, Yemen, Nigeria, India, Malaysia, Ghana, Indonesia, Sudan etc. Malaria cases are registered in bordering areas of the country constantly. The suggested solutions for Malaria control in bordering areas are: 1. To establish control laboratories in customs in order to take blood from persons who come from risky areas for malaria. When positive cases are found these laboratories will also provide free treatment. 2. East country should give information about the malaria situation in their country to the other countries.
    MeSH terms: Animals; Humans; Malaria/epidemiology*; Morbidity/trends; Plasmodium malariae*; Turkey/epidemiology; Incidence; Malaria, Falciparum/epidemiology*; Malaria, Vivax/epidemiology*
  19. Xu ZQ, Norris KJ, Weinberg DS, Kardatzke J, Wertz P, Frank P, et al.
    J Chromatogr B Biomed Sci Appl, 2000 Jun 09;742(2):267-75.
    PMID: 10901131
    A HPLC method was validated for quantification of (+)-calanolide A (1), a novel anti-HIV agent, in rat, dog and human plasma. The synthetic intermediate (+/-)-12-oxocalanolide A (2) was found to be a suitable internal standard. Compounds were extracted from plasma using a solid-phase C(18) cartridge and quantified over the assay range of 12.5 to 800 ng/ml. The method was utilized to determine (+)-calanolide A pharmacokinetics in rats, dogs and humans. This is the first report of a validated HPLC assay for determination of (+)-calanolide A concentrations in rat and dog plasma as well as human plasma obtained from clinical trials. There was no evidence of in vivo epimerization of (+)-calanolide A to its inactive epimer (+)-calanolide B (3).
    MeSH terms: Administration, Oral; Animals; Chromatography, High Pressure Liquid/methods*; Coumarins/administration & dosage; Coumarins/blood*; Coumarins/pharmacokinetics; Dogs; Female; Humans; Male; Reference Standards; Spectrometry, Fluorescence; Reproducibility of Results; Cohort Studies; HIV Seronegativity; Anti-HIV Agents/administration & dosage; Anti-HIV Agents/blood*; Anti-HIV Agents/pharmacokinetics; Area Under Curve; Pyranocoumarins; Rats
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