Fulltext

Development of flexible electrochemical impedance spectroscopy-based biosensing platform for rapid screening of SARS-CoV-2 inhibitors

Kiew LV 1 , Chang CY 2 , Huang SY 3 , Wang PW 4 , Heh CH 5 , Liu CT 6 Show all authors , Cheng CH 7 , Lu YX 7 , Chen YC 7 , Huang YX 7 , Chang SY 8 , Tsai HY 9 , Kung YA 3 , Huang PN 3 , Hsu MH 10 , Leo BF 11 , Foo YY 12 , Su CH 7 , Hsu KC 13 , Huang PH 14 , Ng CJ 15 , Kamarulzaman A 16 , Yuan CJ 17 , Shieh DB 18 , Shih SR 19 , Chung LY 20 , Chang CC 21

Affiliations 

  • 1 Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia; Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan
  • 2 Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan
  • 3 Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan
  • 4 Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 70101, Taiwan
  • 5 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • 6 Division of Nephrology, Department of Internal Medicine, Wan-Fang Hospital, Taipei Medical University, Taipei, 11696, Taiwan; Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, 11031, Taiwan
  • 7 Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan
  • 8 Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan; Bachelor Program in Artificial Intelligence, Chang Gung University, Taoyuan, 33301, Taiwan
  • 9 Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan
  • 10 Department of Chemistry, National Changhua University of Education, Changhua, 50007, Taiwan
  • 11 Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • 12 Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • 13 Pd Biomedical Co., Ltd., Taoyuan, 32560, Taiwan
  • 14 Institute of Clinical Medicine, Cardiovascular Research Center, National Yang Ming Chiao Tung University, Taipei, 11121, Taiwan; Division of Cardiology, Taipei Veterans General Hospital, Taipei, 11217, Taiwan
  • 15 Department of Primary Care Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • 16 Department of Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • 17 Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Institute of Molecular Medicine and Bioengineering, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan
  • 18 Institute of Oral Medicine and Department of Stomatology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, 70101, Taiwan; Center of Applied Nanomedicine, National Cheng Kung University, Tainan, 70101, Taiwan; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan, 70101, Taiwan
  • 19 Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, 33301, Taiwan; Department of Laboratory Medicine, Linkuo Chang Gung Memorial Hospital, Taoyuan, 33301, Taiwan. Electronic address: [email protected]
  • 20 Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: [email protected]
  • 21 Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS(2)B), National Yang Ming Chiao Tung University, Hsinchu, 30068, Taiwan; Department of Electrophysics, National Yang Ming Chiao Tung University, Hsinchu, 30010, Taiwan; Institute of Physics, Academia Sinica, Nankang, Taipei, 11529, Taiwan. Electronic address: [email protected]
Biosens Bioelectron, 2021 Jul 01;183:113213.
PMID: 33857754 DOI: 10.1016/j.bios.2021.113213

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of its spike protein (S-protein) to the cell surface-expressing angiotensin-converting enzyme 2 (ACE2). Thus, inhibition of S-protein-ACE2 binding may impede SARS-CoV-2 cell entry and attenuate the progression of Coronavirus disease 2019 (COVID-19). In this study, an electrochemical impedance spectroscopy-based biosensing platform consisting of a recombinant ACE2-coated palladium nano-thin-film electrode as the core sensing element was fabricated for the screening of potential inhibitors against S-protein-ACE2 binding. The platform could detect interference of small analytes against S-protein-ACE2 binding at low analyte concentration and small volume (0.1 μg/mL and ~1 μL, estimated total analyte consumption 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications