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A coumarin-based fluorescent probe as a central nervous system disease biomarker

Yap AC 1 , Mahamad UA 2 , Lim SY 3 , Kim HJ 4 , Choo YM 5

Affiliations 

  • 1 Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. [email protected]
  • 2 Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. [email protected]
  • 3 Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia. [email protected]
  • 4 Department of Chemistry, Hankuk University of Foreign Studies, Yongin 449-791, Korea. [email protected]
  • 5 Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia. [email protected]
Sensors (Basel), 2014 Nov 10;14(11):21140-50.
PMID: 25390405 DOI: 10.3390/s141121140

Abstract

Homocysteine and methylmalonic acid are important biomarkers for diseases associated with an impaired central nervous system (CNS). A new chemoassay utilizing coumarin-based fluorescent probe 1 to detect the levels of homocysteine is successfully implemented using Parkinson's disease (PD) patients' blood serum. In addition, a rapid identification of homocysteine and methylmalonic acid levels in blood serum of PD patients was also performed using the liquid chromatography-mass spectrometry (LC-MS). The results obtained from both analyses were in agreement. The new chemoassay utilizing coumarin-based fluorescent probe 1 offers a cost- and time-effective method to identify the biomarkers in CNS patients.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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