The endothelial nitric oxide synthase gene G894T, glutathione S-transferase (GSTM1 and GSTT1) polymorphisms as a risk factor in the patient with nephrolithiasis

Mehdi WA 1 , Mehde AA 2 , Yusof F 3 , Raus RA 3 , Resen AK 4 , Ghazali H 5

Affiliations 

  • 1 Sakarya University, Science & Arts Faculty, Department of Chemistry, 54187, Sakarya, Turkey. Electronic address: [email protected]
  • 2 Sakarya University, Science & Arts Faculty, Department of Chemistry, 54187, Sakarya, Turkey
  • 3 International Islamic University Malaysia, College of Engineering, Department of Biotechnology Engineering, 50728 Kuala Lumpur, Malaysia
  • 4 Ministry of Health, Health Care Sector in Rusafa, Iraq
  • 5 Department of Urology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia
Int J Biol Macromol, 2019 Nov 01;140:719-726.
PMID: 31445152 DOI: 10.1016/j.ijbiomac.2019.08.184

Abstract

BACKGROUND: The genetic features indicate a crucial role in nephrolithiasis. The present study was aimed to investigate the role of Glutathione-S-transferase Mu (GSTM1), Glutathione-S- transferase Theta (GSTT1) and endothelial nitric oxide synthase (eNOs) gene polymorphism in nephrolithiasis.

METHODS: We involved a case-control study in which 480 individuals were divided into 240 healthy control and 240 patients with nephrolithiasis. For each patient and control, we measured biochemical criteria, levels of glutathione S-transferase, eNOs, GSTM1, GSTT1genes and eNOS genes polymorphism by PCR-RFLP.

RESULTS: GSTM1 and GSTT1 null genotypes are not a risk features for nephrolithiasis. The eNOS frequency GG, GT, and TT genotypes by using Ban II enzyme as restriction enzyme were found to be (48.33, 36.67, and 15.00) %. The eNOS frequency TT, GT, and GG genotypes by using the Ban II enzyme as restriction enzyme were found to be 15.84, 25.83, and 58.33%, respectively. The result showed an increase in serum eNOs levels were in the patient's group comparing to control.

CONCLUSIONS: This work is the first in the literature to study the relation between eNOs genes polymorphisms and nephrolithiasis. The results conclude that TT genotypes in the eNOs genes are associated with an increase the oxidative stress in patients.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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