Isolation of C-phycocyanin from Spirulina platensis microalga using Ionic liquid based aqueous two-phase system

Chang YK 1 , Show PL 2 , Lan JC 3 , Tsai JC 4 , Huang CR 4

Affiliations 

  • 1 Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan. Electronic address: [email protected]
  • 2 Bioseparation Research Group, Department of Chemical Engineering and Environmental Engineering, University of Nottingham Malaysia Campus, Selangor Darul Ehsan, Malaysia
  • 3 Department of Chemical Engineering and Materials Science, College of Engineering, Yuan Ze University, Taiwan
  • 4 Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City, Taiwan
Bioresour Technol, 2018 Dec;270:320-327.
PMID: 30241065 DOI: 10.1016/j.biortech.2018.07.138

Abstract

An aqueous two-phase system (ATPS) with ionic liquids (ILs) was used for the isolate of C-phycocyanin (CPC) from Spirulina platensis microalga. Various imidazolium ILs and potassium salts were studied. The effect of ILs-ATPS on the extraction efficiency of CPC was also studied. The experimental parameters like pH, loading volume, algae concentration, temperature, and alkyl chain length of IL were well-covered in this report. The experimental results showed that the extraction efficiency, the partition coefficient, and the separation factor for CPC were 99%, 36.6, and 5.8, respectively, for an optimal pH value of 7 and a temperature of 308 K. The order of extraction efficiency for CPC using IL-ATPS was: 1-octyl-3-methylimidazolium bromide (C8MIM-Br) > 1-hexyl-3-methylimidazolium bromide (C6MIM-Br) > 1-butyl-3-methylimidazolium bromide (C4MIM-Br). The isolation process followed the pseudo second-order kinetic model and the thermodynamic results were obviously spontaneous.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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