Purification of lysozyme from chicken egg white by high-density cation exchange adsorbents in stirred fluidized bed adsorption system

Show PL 1 , Ooi CW 2 , Song CP 3 , Chai WS 1 , Lin GT 4 , Liu BL 5 Show all authors , Chang YK 6

Affiliations 

  • 1 Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor, Malaysia
  • 2 Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia; Advanced Engineering Platform, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia
  • 3 Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia
  • 4 Department of Chemical Engineering/ Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan
  • 5 Department of Applied Chemistry, Chaoyang University of Technology, Taichung 413310, Taiwan. Electronic address: [email protected]
  • 6 Department of Chemical Engineering/ Graduate School of Biochemical Engineering, Ming Chi University of Technology, New Taipei City 243303, Taiwan. Electronic address: [email protected]
Food Chem, 2021 May 01;343:128543.
PMID: 33187742 DOI: 10.1016/j.foodchem.2020.128543

Abstract

Lysozyme from crude chicken egg white (CEW) feedstock was successfully purified using a stirred fluidized bed adsorption system ion exchange chromatography where STREAMLINE SP and SP-XL high density adsorbents were selected as the adsorption carrier. The thermodynamic and kinetic studies were carried out to understand the characteristics of lysozyme adsorption by adsorbents under various conditions, including adsorption pH, temperature, lysozyme concentration and salt concentrations. Results showed that SP and SP-XL adsorbents achieved optimum lysozyme adsorption at pH 9 with capacity of ~139.77 and ~251.26 mg/mL, respectively. The optimal conditions obtained from batch studies were directly employed to operate in SFBA process. For SP-XL adsorbent, the recovery yield and purification factor of lysozyme were 93.78% and ~40 folds, respectively. For SP adsorbent, lysozyme can be eluted ~100% with purification factor of ~26 folds. These two adsorbents are highly suitable for use in direct recovery of lysozyme from crude CEW.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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