Effects of salt and heat pre-treatment factors on efficient regeneration in barley (Hordeum vulgare L.)

Haque M 1 , Islam SMS 2 , Subramaniam S 3

Affiliations 

  • 1 Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh
  • 2 Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh. [email protected]
  • 3 School of Biological Sciences, Universiti Sains Malaysia (USM), 11800, Georgetown, Penang, Malaysia
3 Biotech, 2017 May;7(1):63.
PMID: 28452013 DOI: 10.1007/s13205-017-0675-z

Abstract

An efficient callus induction and plant regeneration system has been developed using salt and heat as pre-treatment factors for three barley genotypes viz. BB-3, BB-6 and BHL-18. Different concentrations of NaCl (1.5, 2.5, 3.5, 4.5, 5.5 and 6.5 g/L) were used and its effects were determined on the basis of the viability of callus (CV), plant regeneration (PR), relative growth rate (RGR) and tolerance index (TI). The BB-6 showed highest performance on tolerance based on CV (14.72%), PR (7.69%), RGR (0.91%) and TI (0.42%) at 6.5 g/L NaCl. Various NaCl concentrations displayed significantly differences at P 

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