Fabrication, characterization and application of laccase-nylon 6,6/Fe(3+) composite nanofibrous membrane for 3,3'-dimethoxybenzidine detoxification

Jasni MJ 1 , Sathishkumar P 2 , Sornambikai S 3 , Yusoff AR 4 , Ameen F 5 , Buang NA 1 Show all authors , Kadir MR 3 , Yusop Z 6

Affiliations 

  • 1 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia
  • 2 Centre for Sustainable Environment and Water Security (IPASA), Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia. [email protected]
  • 3 Medical Devices and Technology Group (MEDITEG), Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia
  • 4 Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor Bahru, Malaysia. [email protected]
  • 5 Department of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh, 11451, Saudi Arabia
  • 6 Centre for Sustainable Environment and Water Security (IPASA), Universiti Teknologi Malaysia (UTM), 81310, Johor Bahru, Malaysia
Bioprocess Biosyst Eng, 2017 Feb;40(2):191-200.
PMID: 27757535 DOI: 10.1007/s00449-016-1686-6

Abstract

In this study, laccase was immobilized on nylon 6,6/Fe(3+) composite (NFC) nanofibrous membrane and used for the detoxification of 3,3'-dimethoxybenzidine (DMOB). The average size and tensile strength of the NFC membrane were found to be 60-80 nm (diameter) and 2.70 MPa, respectively. The FTIR results confirm that the amine (N-H) group of laccase was attached with Fe(3+) particles and the carbonyl (C=O) group of NFC membrane via hydrogen bonding. The half-life of the laccase-NFC membrane storage stability was increased from 6 to 11 weeks and the reusability was significantly extended up to 43 cycles against ABTS oxidation. Enhanced electro-oxidation of DMOB by laccase was observed at 0.33 V and the catalytic current was found to be 30 µA. The DMOB-treated mouse fibroblast 3T3-L1 preadipocytes showed maximum (97 %) cell inhibition at 75 µM L(-1) within 24 h. The cytotoxicity of DMOB was significantly decreased to 78 % after laccase treatment. This study suggests that laccase-NFC membrane might be a good candidate for emerging pollutant detoxification.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications