Phyto-synthesis of silver nanoparticles using Alternanthera tenella leaf extract: an effective inhibitor for the migration of human breast adenocarcinoma (MCF-7) cells

Sathishkumar P 1 , Vennila K 2 , Jayakumar R 3 , Yusoff AR 4 , Hadibarata T 1 , Palvannan T 5

Affiliations 

  • 1 Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia
  • 2 Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem, 636 011, Tamil Nadu, India
  • 3 Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia
  • 4 Centre for Environmental Sustainability and Water Security (IPASA), Research Institute for Sustainable Environment (RISE), Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor, Malaysia. [email protected]
  • 5 Laboratory of Bioprocess and Engineering, Department of Biochemistry, Periyar University, Salem, 636 011, Tamil Nadu, India. [email protected]
Bioprocess Biosyst Eng, 2016 Apr;39(4):651-9.
PMID: 26801668 DOI: 10.1007/s00449-016-1546-4

Abstract

In this study, phyto-synthesis of silver nanoparticles (AgNPs) was achieved using an aqueous leaf extract of Alternanthera tenella. The phytochemical screening results revealed that flavonoids are responsible for the AgNPs formation. The AgNPs were characterised using UV-visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray, transmission electron microscopy, fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction. The average size of the nanoparticles was found to be ≈48 nm. The EDX results show that strong signals were observed for the silver atoms. The strong band appearing at 1601-1595 cm(-1) correspond to C-C stretching vibration from dienes in FT-IR spectrum indicating the formation of AgNPs. Human breast adenocarcinoma (MCF-7) cells treated with various concentrations of AgNPs showed a dose-dependent increase in cell inhibition. The IC50 value of the AgNPs was calculated to be 42.5 μg mL(-1). The AgNPs showed a significant reduction in the migration of MCF-7 cells.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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