Browse publications by year: 2024

  1. Yong MY, Tan KY, Tan CH
    PMID: 39579840 DOI: 10.1016/j.cbpc.2024.110077
    High molecular weight proteins are present abundantly in viper venoms. The amino acid sequence can be highly variable though, contributing to the structure and function diversity of snake venom protein. This, however, remains unresolved in many species. The study investigated the venom protein variability in a distinct clade of Asian pit vipers (Trimeresurus species) through comparative proteomics, applying gel electrophoresis (SDS-PAGE), liquid chromatography-tandem mass spectrometry (LCMS/MS), and bioinformatic approaches. The proteomes revealed a number of conserved protein families, within each are variably expressed protein paralogs that are unrelated to the snake phylogeny and geographic origin. The expression levels of two major enzymes, i.e., snake venom serine proteinase and metalloproteinase, correlate weakly with procoagulant and hemorrhagic activities, implying co-expression of other functionally versatile toxins in the venom. The phospholipase A2 (PLA2) abundance correlates strongly with its enzymatic activity, and a unique phenotype was discovered in two species expressing extremely little PLA2. The commercial mono-specific antivenom effectively neutralized the venoms' procoagulant and hemorrhagic effects but failed to inhibit the PLA2 activities. Instead, the PLA2 activities of all venoms were effectively inhibited by the small molecule inhibitor varespladib, suggesting its potential to be repurposed as a highly potent adjuvant therapeutic in snakebite envenoming.
  2. Lee FCH, Sitam FT, Tan LP
    J Virol Methods, 2024 Nov 21.
    PMID: 39580121 DOI: 10.1016/j.jviromet.2024.115074
    DNA samples selected for long read sequencing (LRS) are routinely required to be 'pure' with high DNA concentration. Hence the usefulness of samples with substandard DNA quality for LRS is unknown. We aim to perform de-novo assembly of Adenovirus sequenced from non-human primate (NHP) faeces using the Oxford Nanopore technologies (ONT), an LRS platform. Guided by initial conventional PCR screening, we performed ONT sequencing on 34 Adenovirus positive DNA samples, without prior selection based on faeces freshness level or DNA quality. Non-parametric correlations analysis showed that ONT sequencing outputs is not significantly associated (p > 0.05) with DNA concentrations, faeces freshness levels and the OD ratios of A260/A280 and A260/A230. This indicated that conventional DNA quality parameters may not be the most critical factors in determining the suitability of samples for ONT sequencing. A total of 61.76% (21/34) of the positive-by-PCR-screening samples yielded Adenovirus reads while 38.24% (13/34) did not in the PCR-free ONT workflow, although rarefaction analysis showed that sequencing saturation was achieved by all samples. Among the 21 samples with adenovirus reads, ten resulted in at least one Adenovirus contig by the Flye assembler while nine did not and two samples had only a single Adenovirus read. Identity similarity above 90% in conventional PCR screening may help in selecting ONT positive samples.
  3. Rehman DE, Mohd Ismail ZI, Jawdat D, Ali SA, Sapiai NA, AlAli A
    PMID: 39580556 DOI: 10.1007/s00405-024-09082-1
    BACKGROUND: The sphenoid sinus features many anatomical variations between individuals from different populations in the world. The understanding of these variations is important for the surgeons to plan for surgeries which involve intervention through the nasal approach. The aim of the present systematic review was to perform a qualitative synthesis of available studies which assess the effect of sphenoid sinus (SS) anatomical variations on the outcomes of endoscopic endonasal transsphenoidal surgeries (ETSS).

    METHODS: The current review followed PRISMA 2020 guidelines. A systematic advanced electronic search was performed in four databases Medline (via PubMed), Scopus, Web of Science (WoS) and Lilacs in December 2023. Studies that assessed the anatomical variations of the SS that affect the outcomes of ETSS were eligible for inclusion. A qualitative synthesis of the methodology and results of the included studies was carried out. Quality assessment was performed using the National Institute of Health (NIH) quality assessment tool (last accessed on December 24, 2023).

    RESULTS: A total of 14 studies were included in the qualitative synthesis. Most of the studies included in the review found that the sellar pneumatization was the commonest followed by the postsellar type. Single intersphenoid sinus septum (ISS) was found to be the most common variation, which is more frequently found in males compared to females. There was sex difference seen in the attachment of the ISS to the carotid canal. It is more commonly attached to the posterolateral wall of the sinus in males compared to the females. Though complicated cases were less compared to uncomplicated ones, cerebrospinal fluid (CSF) leak was the leading post operative complication amongst the complicated cases and paranasal sinus (PNS) computed tomography (CT) scan showed ISS findings differed from intraoperative findings.

    CONCLUSION: It is concluded that the ISS poses the commonest anatomical variations encountered during the ETSS. During planning for transsphenoidal endoscopic procedure, the ISS should thoroughly be assessed to minimize potential surgical complications.

  4. Abukhder M, Sahovaler A, Vrakas P, McGurk M, Thavaraj S, Schilling C
    JAMA Otolaryngol Head Neck Surg, 2024 Oct 10;150(11):1021-8.
    PMID: 39388171 DOI: 10.1001/jamaoto.2024.3094
    IMPORTANCE: Frozen section (FS) analysis of sentinel nodes offers potential on-table diagnosis and treatment for occult metastasis in oral squamous cell cancer. Systematic analysis of FS during sentinel node biopsy has not been illuminated in the literature.

    OBJECTIVE: To systematically review pooled data from studies using FS analysis in evaluating sentinel nodes in patients with cT1-T2 N0 oral squamous cell cancer.

    DATA SOURCES: An academic librarian led the search of CENTRAL, CINAHL, Cochrane Database of Systematic Reviews, Embase, and MEDLINE for studies published in English between January 2000 and January 2023.

    STUDY SELECTION: Two authors independently screened cohort studies, case series, and randomized clinical trials, in which FS analysis was used to evaluate sentinel nodes in patients with cT1-T2 N0 oral squamous cell cancer.

    DATA EXTRACTION AND SYNTHESIS: Data were extracted by 2 reviewers. Reporting quality was estimated using the Diagnostic Precision Study Quality Assessment Tool. Data analysis was performed between April and July 2023, and the meta-analysis was completed using the bivariate random-effects model.

    MAIN OUTCOMES AND MEASURES: The primary outcome was the pooled sensitivity of FS sentinel node analysis. Secondary outcomes included evaluation of the FS technique, rate of occult metastasis, false-negative rate, and survival.

    RESULTS: Seventeen articles with 878 patients met the eligibility criteria. Although protocols varied, confirmatory serial step sectioning was performed in all studies. Occult metastasis was found in 263 of 878 patients (30%), and FS analysis identified 173 cases (65.8%). Following serial sectioning, an additional 90 positive results were identified, leading to 47 patients undergoing staged completion neck dissection. The pooled sensitivity of FS was 0.71 (95% CI, 0.60-0.80), the diagnostic odds ratio was 110, and the false-negative rate was 34.2%. The Cochrane Q value was 15.62 (df = 16; P = .48) and τ2 = 0.36.

    CONCLUSION AND RELEVANCE: In this systematic review and meta-analysis, evaluated studies showed various techniques, in which pooled sensitivity reached 0.71, providing a benchmark for comparison to other 1-stop approaches. Due to the high false-negative rate of approximately one-third of patients, intraoperative FS must always be supplemented by serial sectioning. On-table diagnosis remains a key objective for sentinel node biopsy, and FS detection may be improved by standardizing protocols.

  5. Teoh KW, Ng CM, Chong CW, Cheong WL, Ng YL, Bell JS, et al.
    BMJ Open, 2024 Oct 23;14(10):e083921.
    PMID: 39448216 DOI: 10.1136/bmjopen-2024-083921
    INTRODUCTION: Pre-diabetes indicates an elevated risk of developing type-2 diabetes and presents a window for preventive actions. The Pre-diabetes Intervention, Management and Evaluation (PRIME) programme is a community pharmacy-based pre-diabetes management programme that uses a mobile application for self-monitoring and pre-diabetes education, aiming to promote lifestyle changes among participants with pre-diabetes.

    METHODS AND ANALYSIS: This is a protocol for a cluster randomised controlled trial that aims to evaluate the impact of the PRIME programme on participants' clinical outcomes and explore participants' and pharmacists' views towards its implementation. This protocol describes the development of the PRIME programme and mobile app, its feasibility and implementation in community pharmacy settings. 16 pharmacies from two states in Malaysia will be randomised to the intervention arm or standard care. The study will include overweight or obese adults with pre-diabetes. During each follow-up visit at the pharmacy, intervention participants will receive in-depth counselling from pharmacists after reviewing their self-monitoring data recorded in the PRIME app. They will also receive pre-diabetes education through the app and join a peer support chatgroup. The primary clinical outcome includes changes in body weight at 6 months, while the secondary clinical outcomes include changes in blood glucose profile, lipid profile, blood pressure and adiposity measures. The sustainability of the PRIME programme will be accessed using a follow-up questionnaire, while participants' engagement with the intervention will be evaluated using attendance rate and the app data. Focus group discussions and one-to-one interviews will be conducted for process evaluation. This study will inform the impact of community pharmacists-led digital health intervention in pre-diabetes management.

    ETHICS AND DISSEMINATION: This study has been registered with clinicaltrials.gov (NCT04832984) and approved by the Monash University Human Research Ethics Committee (Project ID: 27512).

    TRIAL REGISTRATION NUMBER: clinicaltrials.gov (NCT04832984).

    MeSH terms: Adult; Diabetes Mellitus, Type 2/prevention & control; Diabetes Mellitus, Type 2/therapy; Female; Humans; Life Style; Malaysia; Male; Obesity/prevention & control; Obesity/therapy; Pharmacists; Randomized Controlled Trials as Topic; Telemedicine; Mobile Applications
  6. Ong SH, Thomson AB, Wright NE, Nic Ionmhain U, Roberts DM
    Clin Toxicol (Phila), 2024 Nov;62(11):770-775.
    PMID: 39466316 DOI: 10.1080/15563650.2024.2412203
    INTRODUCTION: In 2015, Australia and New Zealand treatment guidelines recommended a 2 h paracetamol serum concentration for risk assessment of unintentional paracetamol liquid exposures. We assess our experience with this approach.

    METHODS: Retrospective case review of children <6 years-old with liquid paracetamol overdoses referred to a regional poisons information centre January 2017 to August 2022. We extracted data on the exposure and management from the poisons information centre and hospital medical records. We identified additional cases with two paracetamol concentrations obtained from September 2022 to June 2024.

    RESULTS: Of 437 paediatric poisonings, 271 were eligible for inclusion. The median age was 24 months, the median time to presentation was 120 min, and paracetamol was the sole ingestant in 92% of cases. Blood testing was recommended in 131 patients (48.3%), occurring at 2 h post-ingestion in 62 patients (47.3%). Testing at a later time was mostly due to delayed presentation, including to hospitals unable to measure paracetamol concentrations. Eighteen patients (16.7%) had repeat blood testing, and five additional cases were identified in the subsequent period. Overall, the concentration decreased in 19 patients (83%), but in three patients it increased, from 73 mg/L to 81 mg/L (0.49-0.54 mmol/L), from 154 mg/L to 179 mg/L (1.03-1.19 mmol/L), and from 56 mg/L to 115 mg/L (0.37-0.77 mmol/L). Symptomatic patients were more likely to receive a second blood test or acetylcysteine while awaiting investigations. Of 19 patients administered acetylcysteine, it was discontinued in five due to low paracetamol serum concentrations. All patients recovered.

    DISCUSSION: Guidelines were followed in >90% of patients and this testing regimen shortened length of stay. Based on these data, Australian treatment guidelines now recommend repeat testing for 2 h paracetamol serum concentrations >100 mg/L (0.67 mmol/L).

    CONCLUSION: A paracetamol serum concentration between 2 h and 4 h post-ingestion in children <6 years-old with unintentional poisonings of paracetamol liquid can facilitate medical discharge.

    MeSH terms: Australia; Child, Preschool; Female; Humans; Infant; Male; New Zealand; Poison Control Centers*; Retrospective Studies; Practice Guidelines as Topic*; Analgesics, Non-Narcotic/blood; Analgesics, Non-Narcotic/poisoning
  7. Lagutkin D, Panaifo L, Nurul-Aain AF, Israelsson L, Hansson M, Lundberg K, et al.
    ACR Open Rheumatol, 2024 Nov 22.
    PMID: 39576054 DOI: 10.1002/acr2.11767
    OBJECTIVE: Autoantibodies serve as essential clinical biomarkers and may indicate etiological mechanisms in rheumatic diseases. In light of the increasing knowledge concerning the diversity and biologic implications of anti-citrullinated peptide/protein antibodies (ACPAs), we have re-evaluated the association between the ACPA response and the HLA-DRB1 allelic groups, known to represent a major genetic risk factor for rheumatoid arthritis (RA).

    METHODS: We explored a collection of 4,392 well-characterized incident patients with RA of White European descent from the Epidemiological Investigation of Rheumatoid Arthritis (EIRA) new-onset RA study, as well as 1,199 cases of patients with RA of Southeast Asian origin from the Malaysian EIRA study. We focused on a quantitative analysis of the levels of anti-cyclic citrullinated peptide IgG antibodies, including those falling below the diagnostic threshold.

    RESULTS: Our data show that non-shared epitope alleles HLA-DRB1*09 and *15 exhibit significant associations with ACPA levels. Notably, these novel associations were independent of ethnicity. To validate our findings, we conducted an additional replication study in an independent pool of 4,109 patients with RA of White European origin.

    CONCLUSION: These results indicate a new, previously overlooked, role for the HLA locus in the regulation of the levels of ACPA RA-specific autoantibodies that goes beyond the shared epitope-defined gene variants.

  8. Papot E, Tovar-Sanchez T, Woods J, Thaurignac G, Eriobu N, Borok M, et al.
    AIDS, 2024 Nov 22.
    PMID: 39576151 DOI: 10.1097/QAD.0000000000004068
    OBJECTIVE: Data on the impact of COVID-19 in people living with HIV (PWH) are lacking in resource-constrained settings. We utilised existingrandomised clinical trials (RCTs) on antiretroviral therapies (ART) in HIV-1 infection to conduct a SARS-CoV-2 serosurvey, between January and March 2021, while characterising participants' features.

    DESIGN: Cross-sectional serosurvey.

    METHODS: Demographic characteristics, medical history and a serum sample were collected from consenting PWH. Samples were analysed centrally for immunoglobulin G antibodies to recombinant nucleocapsid and spike proteins derived from SARS-CoV-2 using a Luminex based assay.

    RESULTS: The 549participants recruited in 9 sites across Africa had a median age of 40 years (IQR [34-45]); 63.0% (346) were female. All were on ART; 81.8% (449) had an HIV-1 viral load <50 copies/mL, with CD4 count median at 478/mm 3 (IQR [320-677]). None had received vaccination against SARS-CoV-2. Forty participants (7.3%) had a prior SARS-CoV-2 PCR testing, of whom 10 were positive (1.8%). Crude SARS-CoV-2 seroprevalence was 36.2% (; 95%CI [32.2-40.4]). In the explorative multivariable analysis, comparison of the characteristics of PWH with a positive SARS-CoV-2 serology with those with a negative or indeterminate serology: PWH with a body mass index (BMI)≥30 kg/m 2 were more likely to have a positive serology than those with a BMI≤25 (aOR = 2.39 [1.48-3.86], p 

  9. Fauzi AAB, Chitraningrum N, Budiman I, Subyakto S, Widyaningrum BA, Maheswari CS, et al.
    PMID: 39576437 DOI: 10.1007/s11356-024-35589-5
    A promising water treatment method involves using biomass-derived activated carbon (AC) to remove emerging pollutants from wastewater due to its adsorption capacity, cost-effectiveness, and sustainability. Notwithstanding, the existing literature lacks comprehensive studies that specifically focus on removing contaminants in water by comparing the effectiveness of adsorption and photocatalytic degradation methods. Additionally, there is not much emphasis on analyzing the combined processes of adsorption-photocatalytic degradation utilizing AC. Herein, this paper investigates the intricacies of adsorption-photocatalytic degradation mechanisms and contributing variables in the enhancement of performances using biomass-derived AC. Furthermore, this review paper presents a comprehensive examination of different biomass sources employed in the synthesis of AC. It also discusses the diverse techniques utilized for the fabrication of AC, including physical and chemical activation methods. Finally, the shortcomings and future prospects of biomass-derived AC have been addressed. This study offers significant insights for the development of future biomass-derived AC, with the goal of improving their efficiency and expanding their uses in wastewater treatment.
  10. Levis B, Bhandari PM, Neupane D, Fan S, Sun Y, He C, et al.
    JAMA Netw Open, 2024 Nov 04;7(11):e2429630.
    PMID: 39576645 DOI: 10.1001/jamanetworkopen.2024.29630
    IMPORTANCE: Test accuracy studies often use small datasets to simultaneously select an optimal cutoff score that maximizes test accuracy and generate accuracy estimates.

    OBJECTIVE: To evaluate the degree to which using data-driven methods to simultaneously select an optimal Patient Health Questionnaire-9 (PHQ-9) cutoff score and estimate accuracy yields (1) optimal cutoff scores that differ from the population-level optimal cutoff score and (2) biased accuracy estimates.

    DESIGN, SETTING, AND PARTICIPANTS: This study used cross-sectional data from an existing individual participant data meta-analysis (IPDMA) database on PHQ-9 screening accuracy to represent a hypothetical population. Studies in the IPDMA database compared participant PHQ-9 scores with a major depression classification. From the IPDMA population, 1000 studies of 100, 200, 500, and 1000 participants each were resampled.

    MAIN OUTCOMES AND MEASURES: For the full IPDMA population and each simulated study, an optimal cutoff score was selected by maximizing the Youden index. Accuracy estimates for optimal cutoff scores in simulated studies were compared with accuracy in the full population.

    RESULTS: The IPDMA database included 100 primary studies with 44 503 participants (4541 [10%] cases of major depression). The population-level optimal cutoff score was 8 or higher. Optimal cutoff scores in simulated studies ranged from 2 or higher to 21 or higher in samples of 100 participants and 5 or higher to 11 or higher in samples of 1000 participants. The percentage of simulated studies that identified the true optimal cutoff score of 8 or higher was 17% for samples of 100 participants and 33% for samples of 1000 participants. Compared with estimates for a cutoff score of 8 or higher in the population, sensitivity was overestimated by 6.4 (95% CI, 5.7-7.1) percentage points in samples of 100 participants, 4.9 (95% CI, 4.3-5.5) percentage points in samples of 200 participants, 2.2 (95% CI, 1.8-2.6) percentage points in samples of 500 participants, and 1.8 (95% CI, 1.5-2.1) percentage points in samples of 1000 participants. Specificity was within 1 percentage point across sample sizes.

    CONCLUSIONS AND RELEVANCE: This study of cross-sectional data found that optimal cutoff scores and accuracy estimates differed substantially from population values when data-driven methods were used to simultaneously identify an optimal cutoff score and estimate accuracy. Users of diagnostic accuracy evidence should evaluate studies of accuracy with caution and ensure that cutoff score recommendations are based on adequately powered research or well-conducted meta-analyses.

    MeSH terms: Cross-Sectional Studies; Depression/diagnosis; Depressive Disorder, Major/diagnosis; Female; Humans; Male; Mass Screening/methods; Sensitivity and Specificity
  11. Abdull Rahim U, Mustapa M, Mohamed Shakrin NNS, Nurdin A, Mohamad Taridi N, Yusof YAM, et al.
    PLoS One, 2024;19(11):e0314280.
    PMID: 39576841 DOI: 10.1371/journal.pone.0314280
    Cervical cancer ranked fourth most common malignancy among women worldwide despite the establishment of vaccination programmes. This systematic review evaluates the anti-cancer properties of turmeric and ginger bioactive compounds, specifically curcumin, 6/10-gingerol, and 6/10-shogaol, and their combination in cervical cancer through in-vitro and in-vivo models. A comprehensive electronic search was performed using Science Direct, PubMed, and Scopus from inception until the second week of June 2024 for studies published in English. Only studies investigating the effects of curcumin, gingerol, shogaol, and/or their combination in human cervical cancer cell lines and/or rodent animal models implanted with cervical cancer xenografts were included. Altogether, 27 studies were included in this review. The evidence gathered indicated that curcumin, 6/10-gingerol and 6-shogaol exert their anticancer action through modulation of cell signalling pathways, including AMPK, WNT, PI3K/AKT, and NF-κB pathway, and mediators including Bax/Bcl2, TNF-α, EGFR, COX-2, caspases-3, -9, p53, and pRb. However, the synergistic effect of these bioactive compounds is not known due to lack of evidence. In conclusion, curcumin, 6/10-gingerols, and 6-shogaols hold promise as therapeutic agents for cervical cancer. Yet, further research is essential to understand their combined efficacy, emphasising the need for additional studies exploring the synergistic anticancer effects of these bioactive compounds. Additional factors to explore include long-term effects and susceptibility of chemoresistant cervical cancer cells towards curcumin, shogaols, and gingerols.
    MeSH terms: Animals; Antineoplastic Agents/pharmacology; Antineoplastic Agents/therapeutic use; Female; Humans; Signal Transduction/drug effects; Ginger/chemistry; Cell Line, Tumor
  12. Hayrapetyan A, Tumasyan A, Adam W, Andrejkovic JW, Bergauer T, Chatterjee S, et al.
    Phys Rev Lett, 2024 Nov 08;133(19):191902.
    PMID: 39576923 DOI: 10.1103/PhysRevLett.133.191902
    The first search for soft unclustered energy patterns (SUEPs) is performed using an integrated luminosity of 138  fb^{-1} of proton-proton collision data at sqrt[s]=13  TeV, collected in 2016-2018 by the CMS detector at the LHC. Such SUEPs are predicted by hidden valley models with a new, confining force with a large 't Hooft coupling. In events with boosted topologies, selected by high-threshold hadronic triggers, the multiplicity and sphericity of clustered tracks are used to reject the background from standard model quantum chromodynamics. With no observed excess of events over the standard model expectation, limits are set on the cross section for production via gluon fusion of a scalar mediator with SUEP-like decays.
  13. Yip YY, Makmor-Bakry M, Chong WW
    J Med Internet Res, 2024 Nov 22;26:e59742.
    PMID: 39576982 DOI: 10.2196/59742
    BACKGROUND: The high prevalence of noncommunicable diseases and the growing importance of social media have prompted health care professionals (HCPs) to use social media to deliver health information aimed at reducing lifestyle risk factors. Previous studies have acknowledged that the identification of elements that influence user engagement metrics could help HCPs in creating engaging posts toward effective health promotion on social media. Nevertheless, few studies have attempted to comprehensively identify a list of elements in social media posts that could influence user engagement metrics.

    OBJECTIVE: This systematic review aimed to identify elements influencing user engagement metrics in social media posts by HCPs aimed to reduce lifestyle risk factors.

    METHODS: Relevant studies in English, published between January 2006 and June 2023 were identified from MEDLINE or OVID, Scopus, Web of Science, and CINAHL databases. Included studies were those that examined social media posts by HCPs aimed at reducing the 4 key lifestyle risk factors. Additionally, the studies also outlined elements in social media posts that influenced user engagement metrics. The titles, abstracts, and full papers were screened and reviewed for eligibility. Following data extraction, narrative synthesis was performed. All investigated elements in the included studies were categorized. The elements in social media posts that influenced user engagement metrics were identified.

    RESULTS: A total of 19 studies were included in this review. Investigated elements were grouped into 9 categories, with 35 elements found to influence user engagement. The 3 predominant categories of elements influencing user engagement were communication using supportive or emotive elements, communication aimed toward behavioral changes, and the appearance of posts. In contrast, the source of post content, social media platform, and timing of post had less than 3 studies with elements influencing user engagement.

    CONCLUSIONS: Findings demonstrated that supportive or emotive communication toward behavioral changes and post appearance could increase postlevel interactions, indicating a favorable response from the users toward posts made by HCPs. As social media continues to evolve, these elements should be constantly evaluated through further research.

    MeSH terms: Health Personnel/psychology; Health Promotion/methods; Humans; Life Style*; Risk Factors; Social Media*
  14. Singh VA, Ong BK, Yasin NF
    Musculoskelet Surg, 2024 Dec;108(4):483-489.
    PMID: 38848000 DOI: 10.1007/s12306-024-00840-2
    BACKGROUND: Major musculoskeletal oncology procedures often result in perioperative bleeding. This exposes patients to allogeneic red blood cell transfusion and its potential complications, thus increasing the risk of surgical wound infection and prolonged hospital stay. This study aimed to investigate the efficacy of oxidised cellulose, a topical haemostatic agent, in reducing postoperative blood loss and its subsequent risks.

    METHODS: In this randomised controlled trial, 40 patients undergoing major musculoskeletal oncology procedures were assigned to control and intervention groups. Oxidised cellulose was inserted into the surgical wound after the resection's conclusion before the wound's closure to reduce postoperative bleeding for patients in the intervention group. Postoperative closed suction drain system (Redivac TM) volume, drop in haemoglobin level, allogeneic red blood cell transfusion rate, duration of surgery, and length of hospital stay were compared between the two groups.

    RESULTS: The postoperative Redivac volume (Control: 432 MLS vs. Intervention: 431.75 MLS), drop in haemoglobin level (Control: 3.12 g/dL vs. Intervention: 3.06 g/dL), duration of surgery (Control: 134 vs. Intervention: 156 min), and allogeneic red blood cell transfusion were lower in the intervention group (Control: 204 MLS vs. Intervention: 170 MLS), but they were not statistically significant (p > 0.05) (Control: 134 vs. Intervention: 156 min). Mean hospital stay was similar in both groups (Control: 5.45 days vs. Intervention: 5.85 days).

    CONCLUSION: Oxidised cellulose use does not significantly affect postoperative blood loss, the rate of allogeneic blood transfusion, and hospital stay. However, we believe its use contributes positively but not considerably towards lower postoperative blood loss in musculoskeletal oncology surgeries.

    MeSH terms: Adult; Aged; Bone Neoplasms/surgery; Female; Hemoglobins/analysis; Hemostatics/therapeutic use; Humans; Length of Stay; Male; Middle Aged; Erythrocyte Transfusion; Operative Time
  15. Munawar K, Mukhtar F, Roy M, Majeed N, Jalaludin MY
    Psychol Health Med, 2024 Dec;29(10):1705-1752.
    PMID: 39467823 DOI: 10.1080/13548506.2024.2421461
    This systematic review evaluates studies focusing on parenting and feeding practices, children's feeding behavior, and growth stunting in Asian countries. Eight electronic databases were searched to screen studies published between Jan 2015 and May 2021. There were contradictory findings related to gender differences in growth stunting and factors that reinforce/facilitate or inhibit acquisition of optimum growth in children. Theme of parenting practices showed that time spent on childcare, traditional beliefs about child-care feeding and responsive feeding were also contributing factors. Amidst economic improvement, political, social changes, and worldwide execution of various nutritional programs, stunting continues to be relentlessly persistent and widespread in Asia. Undernutrition disturbs areas of the mind involved in reasoning, reminiscence, locomotor skills and also an adverse influence on the physical and psychological growth of children and ensuing learning capabilities. Stunted children have more anxiety and depression and lower self-esteem than non-stunted children. A public health strategy is required to: (i) properly examine stunting with time through collaborated efforts of community members and various sectors, (ii) tackle malnutrition with steps to enhance maternal nutrition during pregnancy, infant feeding practices and (iii) involvement of effective multi-sectoral partnership programs for management of stunting.
    MeSH terms: Asia; Child; Child, Preschool; Female; Humans; Infant; Male
  16. van der Laan L, Silva A, Kleinendorst L, Rooney K, Haghshenas S, Lauffer P, et al.
    HGG Adv, 2024 Nov 04;6(1):100380.
    PMID: 39501558 DOI: 10.1016/j.xhgg.2024.100380
    Neurodevelopmental disorder with or without autism or seizures (NEDAUS) is a neurodevelopmental disorder characterized by global developmental delay, speech delay, seizures, autistic features, and/or behavior abnormalities. It is caused by CUL3 (Cullin-3 ubiquitin ligase) haploinsufficiency. We collected clinical and molecular data from 26 individuals carrying pathogenic variants and variants of uncertain significance (VUS) in the CUL3 gene, including 20 previously unreported cases. By comparing their DNA methylation (DNAm) classifiers with those of healthy controls and other neurodevelopmental disorders characterized by established episignatures, we aimed to create a diagnostic biomarker (episignature) and gain more knowledge of the molecular pathophysiology. We discovered a sensitive and specific DNAm episignature for patients with pathogenic variants in CUL3 and utilized it to reclassify patients carrying a VUS in the CUL3 gene. Comparative epigenomic analysis revealed similarities between NEDAUS and several other rare genetic neurodevelopmental disorders with previously identified episignatures, highlighting the broader implication of our findings. In addition, we performed genotype-phenotype correlation studies to explain the variety in clinical presentation between the cases. We discovered a highly accurate DNAm episignature serving as a robust diagnostic biomarker for NEDAUS. Furthermore, we broadened the phenotypic spectrum by identifying 20 new individuals and confirming five previously reported cases of NEDAUS.
  17. Mohamed Tahir AR, Haw VJ, Zaid WSW, Nizaruddin MA, Abd Razak FS, Ludin AFM
    Turk J Pharm Sci, 2024 Nov 21;21(5):413-420.
    PMID: 39569669 DOI: 10.4274/tjps.galenos.2023.23428
    OBJECTIVES: To promote a holistic approach to healthcare, the University of Cyberjaya introduced a unique elective course called sports pharmacy, which incorporates components of lifestyle interventions. Customers increasingly seek guidance about lifestyle factors that impact their health. Pharmacists with expertise in sports and exercise can meet this need. However, there is a lack of studies on the impact of non-pharmacological approaches (NPAs) on health parameters among healthcare professionals and pharmacy students in Malaysia. The objectives of this study were to compare students' health parameters based on NPAs and identify the factors that motivate students to maintain their health.

    MATERIALS AND METHODS: The study comprised 47 Year 4 pharmacy students in the pre- and post-practical phases (September 2018 and November 2018), followed by the post-resting phase (May 2019). The data collection form in the Sport Pharmacy course was used for the data collection.

    RESULTS: Nearly half of the students initially displayed a normal body mass index (BMI). However, after the post-resting period, there was a noticeable increase in the number of students categorized as obese and those with elevated total cholesterol (TC) and fasting blood glucose (FBG) levels. Specifically, in the pre-study phase, out of 47 participants, 22 were within the normal BMI (47%), six underweight (13%), nine overweight (19%), and ten obese (21%). The intervention phase showed a slight reduction in the overweight category, while the obese category showed an increase. For TC, the pre-phase had 70% of participants within the normal range, with 26% borderline high and 4% high. Post-study, showed an improvement, likely influenced by controlled dietary intake and physical activity. In the post-resting phase, however, there was a regression as the majority did not adhere to the non-pharmacological regimen. FBG demonstrated significant changes after the intervention, particularly within the normal range (≤ 6.0 mmol/L), showing the only statistically significant change across parameters. While the post-resting phase saw a minor increase, it remained below baseline. Approximately 23% continued diet control, while 32% maintained physical activity. Key motivations included health improvement, visible results, and improved well-being, while lack of motivation, time, and study schedules were primary discontinuation factors.

    CONCLUSION: The 10-week intervention significantly impacted FBG but had limited influence on BMI and TC. Post-resting outcomes highlight that only a small fraction maintained the non-pharmacological approach, resulting in no marked changes in any parameters. Recommendations include further long-term studies to confirm the sustained benefits and the role of educational institutions in supporting such interventions.

  18. Darbandi A, Koupaei M, Kiani P, Ghanavati R, Najafi P, Hosseini J, et al.
    Immun Inflamm Dis, 2024 Nov;12(11):e70076.
    PMID: 39570098 DOI: 10.1002/iid3.70076
    BACKGROUND: Despite the advent of vaccines against COVID-19, there is considerable variation in the acceptance and hesitancy towards the vaccination program across different countries. The objective of this study was to ascertain the prevalence of hesitancy and acceptance regarding the use of the vaccine against the novel coronavirus, also known as COVID-19, and to identify the factors that influence these attitudes.

    MATERIALS AND METHODS: All the cross-sectional studies were retrieved from the PubMed databases, the Web of Science ISI, Scopus, and the Cochrane Library. Papers published in English between 2 November 2019 and 23 May 2023 were subjected to further assessment based on their title, abstract, and main text, with a view to ensuring their relevance to the present study.

    RESULTS: Following an exhaustive investigation, 59 studies were selected for screening in this systematic review. The most frequently employed method of data collection was the online survey. The study sample comprised 59.12% women and 40.88% men, with ages ranging from 16 to 78 years. The proportion of individuals accepting the vaccine ranged from 13% to 96%, while the proportion of those exhibiting hesitancy ranged from 0% to 57.5%. The primary reasons for accepting the COIVD-19 vaccine were a heightened perception of risk associated with the virus and a general trust in the healthcare system. The most frequently cited reasons for vaccine hesitancy in the context of the ongoing pandemic include concerns about the potential dangers of the vaccines, the rapid pace of their development, the possibility of adverse effects (such as infertility or death), and the assumption that they have been designed to inject microchips.

    DISCUSSION: A variety of socio-demographic factors are implicated in determining the rate of vaccine acceptance. A number of socio-demographic factors have been identified as influencing vaccine acceptance. These include high income, male gender, older age, marriage, the presence of older children who have been vaccinated and do not have chronic diseases, high education, and health insurance coverage.

    CONCLUSION: Eliminating vaccine hesitancy or increasing vaccine acceptance is a crucial factor that should be addressed through various means and in collaboration with regulatory and healthcare organizations.

    MeSH terms: Adolescent; Adult; Aged; Cross-Sectional Studies; Female; Humans; Health Knowledge, Attitudes, Practice; Male; Middle Aged; Young Adult
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