Browse publications by year: 1998

  1. Ng TP, Seow A, Tan WC
    Eur Respir J, 1998 Jul;12(1):198-203.
    PMID: 9701437
    This study investigated the prevalence of snoring and symptoms of sleep breathing-related disorders in the multi-ethnic population of Singapore (3 million people, comprising 75% Chinese, 15% Malay and 7% Indian). A multistaged, area cluster, disproportionate stratified, random sampling of adults aged 20-74 yrs was used to obtain a sample of 2,298 subjects (65% response), with approximately equal numbers of Chinese, Malay and Indian and in each 10 yr age group. An interviewer-administered field questionnaire was used to record symptoms of snoring and breathing disturbances during sleep witnessed by a room-mate and other personal and health-related data. The weighted point estimate (and 95% confidence interval) of the whole population prevalence of snoring was 6.8% (53-83). There were pronounced ethnic differences among Chinese, 6.2% (4.4-8.1); Malay, 8.1% (6.1-10.2) and Indian, 10.9% (85-13.4). The minimum whole population prevalence by the most restricted symptom criteria for defining sleep breathing-related disorder was 0.43% (0.05-0.8%). Similar marked ethnic differences in rates were observed using various symptom criteria. The ethnic differences in sleep breathing symptoms paralleled the differences in body mass index, neck circumference and hypertension, but statistically significant differences remained after adjustment for sex, age and these known associated factors. Marked ethnic differences in snoring and sleep breathing-related disorders were observed in Chinese, Malays and Indians in Singapore, which were only partly explained by known factors of sex, age and body habitus.
    MeSH terms: Adult; China/ethnology; Cross-Sectional Studies; Cross-Cultural Comparison; Ethnic Groups/statistics & numerical data*; Humans; India/ethnology; Malaysia/ethnology; Middle Aged; Risk Factors; Singapore/epidemiology; Sleep Apnea Syndromes/ethnology*; Sleep Apnea Syndromes/epidemiology; Snoring/ethnology*; Snoring/epidemiology; Incidence; Cluster Analysis
  2. Vadivelu J, Puthucheary SD, Drasar BS, Dance DA, Pitt TL
    Trop Med Int Health, 1998 Jul;3(7):518-21.
    PMID: 9705184
    The constancy of strain genotypes of multiple isolates of Burkholderia pseudomallei from 13 patients with melioidosis was examined by BamHI ribotyping and pulsed-field gel electrophoresis (PFGE) of XbaI digests of DNA. Seven of 8 patients with single episodes of melioidosis each yielded genetically identical isolates and only one of five patients with recurrent episodes was infected with a new strain clearly distinct from the original primary strain. Variation was observed in PFGE patterns of primary and relapse isolates of another patient but this was insufficient to define genetically distinct strains. We conclude that most patients with single or multiple episodes of melioidosis retain a single strain.
    MeSH terms: Genotype; Humans; Melioidosis/microbiology*; Recurrence; RNA, Bacterial/genetics; RNA, Ribosomal/genetics; Serotyping/methods; Restriction Mapping; Electrophoresis, Gel, Pulsed-Field; Burkholderia pseudomallei/classification; Burkholderia pseudomallei/genetics*; Burkholderia pseudomallei/isolation & purification
  3. Hawariah A, Stanslas J
    In Vivo, 1998 Jul-Aug;12(4):403-10.
    PMID: 9706492
    Early studies reported that a styrylpyrone derivative (SPD) purified from the Goniothalamus sp. acts as a non-competitive antiestrogen in early pregnant mice (1). In the immature rat uterine wet weight test, we found that SPD markedly reduced uterine weight at doses 1 and 100 mg/kg, thus reflecting negative antiestrogenicity, probably attributed to low binding affinities towards ER. Tamoxifen (Tam) on the other hand exhibited partial antiestrogenicity at all doses (0.01-10 mg/kg BW) and dose-dependent estrogenicity. However, the estrogen antagonism: agonism ratio for SPD is much higher than Tam, which is indicative of the breast cancer antitumor activity as seen in compounds such as MER-25. Pretreatment assessment on 1 mg/kg BW SPD and Tam showed that SPD is not a very good, estrogen antagonist compared to Tam, as it was unable to revert the estrogenicity effect of estradiol benzoate (EB) on immature rat uterine weight. Antitumor activity assessment for SPD exhibited significant tumor growth retardation in 7,12-dimethyl benzanthracene (DMBA) induced rat mammary tumors at all doses employed (2, 10 and 50 mg/kg) compared to the controls (p < 0.01). This compound was found to be more potent than Tam (2 and 10 mg/kg) and displayed greater potency at a dose of 10 mg/kg. It caused complete remission of 33.3% of tumors but failed to prevent onset of new tumors. However, SPD administration at 2 mg/kg caused 16.7% complete remission and partial remission. It also prevented the onset of new tumors throughout the experiment.
    MeSH terms: Animals; Antineoplastic Agents/pharmacology*; Carcinogens/pharmacology; Female; Mammary Neoplasms, Experimental/chemically induced; Mammary Neoplasms, Experimental/drug therapy*; Mammary Neoplasms, Experimental/pathology; Organ Size; Pyrones/pharmacology*; Tamoxifen/pharmacology; Uterus; 9,10-Dimethyl-1,2-benzanthracene; Rats, Sprague-Dawley; Styrene/pharmacology*; Rats
  4. Loh HS, Ling SY, Shanmuhasuntharam P, Zain R, Yeo JF, Khoo SP
    Aust Dent J, 1998 Jun;43(3):188-91.
    PMID: 9707784
    This survey was undertaken to study the clinical features of trigeminal neuralgia in an Asian population. Demographic data of 44 patients treated at the Dental Faculty of the National University of Singapore and at the University of Malaya were reviewed. The results of the survey were analysed and comparisons made with those of Caucasian patients as reported in other studies where there was a general similarity in the clinical findings. Trigeminal neuralgia presented predominantly in females. Right-sided involvement occurred at a greater frequency, and the peak age at onset was between the sixth and seventh decades of life. The only significant variant in the present sample was the greater involvement of the mandibular branch of the trigeminal nerve rather than the maxillary division. In addition, there was a much greater representation from Chinese patients over Malays as compared with their ratios in the general population.
    MeSH terms: Adult; Aged; Aged, 80 and over; China/ethnology; Female; Humans; Malaysia/ethnology; Malaysia/epidemiology; Male; Mandibular Nerve/physiopathology; Maxillary Nerve/physiopathology; Middle Aged; Retrospective Studies; Sex Factors; Singapore/epidemiology; Trigeminal Neuralgia/ethnology; Trigeminal Neuralgia/epidemiology*; Age of Onset; European Continental Ancestry Group; Asian Continental Ancestry Group
  5. Deshmukh RG, Lou KK, Neo CB, Yew KS, Rozman I, George J
    Injury, 1998 Apr;29(3):207-10.
    PMID: 9709422
    Closed intramedullary nailing is a successful method of treating adult femoral shaft fractures. In comminuted or segmental fractures, this operation is associated with an incidence of rotational malalignment and malunion. After locked nailing, this can only be corrected by further operation. A simple method of judging and obtaining rotational alignment in such cases intra-operatively is described. A comparison of two groups of patients with such fractures, one using this technique and the other using conventional methods of judging alignment, revealed statistically significant improvement in rotational alignment (p = 0.016).
    MeSH terms: Adolescent; Adult; Bone Nails*; Female; Femoral Fractures/surgery*; Fracture Fixation, Intramedullary/methods*; Fractures, Closed/surgery*; Humans; Male; Prospective Studies; Fracture Healing
  6. Abdullah BJ, Teong LK, Mahadevan J, Jalaludin A
    J Otolaryngol, 1998 Aug;27(4):190-4.
    PMID: 9711512
    Impacted foreign bodies in the trachea and esophagus are common. In the esophagus, these are usually bones, while for the trachea, it is commonly some form of seed or nut. The incidence of impacted dental prostheses is not highlighted in the literature. They usually have a definite history of ingestion, frequently during trauma, seizures, or sleep. Prompt management in a safe and effective manner is required if significant morbidity, and even mortality, is to be avoided.
    MeSH terms: Adult; Aged; Aged, 80 and over; Denture, Partial*; Endoscopy; Esophagus/radiography*; Female; Foreign Bodies/complications; Foreign Bodies/radiography*; Humans; Larynx/radiography*; Male; Middle Aged; Oropharynx/radiography*
  7. Radu S, Elhadi N, Hassan Z, Rusul G, Lihan S, Fifadara N, et al.
    FEMS Microbiol Lett, 1998 Aug 01;165(1):139-43.
    PMID: 9711850
    Antibiotic susceptibility, plasmid profiles and random amplification of polymorphic DNA (RAPD) were used to study strains of Vibrio vulnificus isolated from cockles (Anadara granosa). Thirty-six isolates were analyzed. The prevalent biotypes were 1 (72.2% of the isolates) and 2 (27.8%). Among these, 21 strains of biotype 1 and two strains of biotype 2 contained plasmid DNA bands ranging in size from 1.4 to 9.7 MDa. Thirty-one (83.3%) were found to be resistant to one or more of the antimicrobial agents tested, however no specific correlation between antimicrobial resistance patterns and a single biotype was found. In addition, no particular plasmid profile was predictive of a particular pattern of antibiotic susceptibility. Two primers produced polymorphisms in all strains tested, producing bands ranging from 0.25 to 2.7 kb, indicating a high variability among both biotype 1 and biotype 2 of the V. vulnificus strains investigated. RAPD identity across biotypes was also observed among Vibrio vulnificus strains.
    MeSH terms: Animals; Anti-Bacterial Agents/pharmacology; DNA, Bacterial/analysis; DNA, Bacterial/genetics; Drug Resistance, Microbial; Kanamycin Resistance; Penicillin Resistance; Plasmids/genetics*; Shellfish/microbiology*; Vibrio/classification; Vibrio/drug effects*; Vibrio/genetics*; Vibrio/isolation & purification; Random Amplified Polymorphic DNA Technique
  8. Liam CK, Lim KH
    Int J Tuberc Lung Dis, 1998 Aug;2(8):683-9.
    PMID: 9712285
    University of Malaya Medical Centre, Kuala Lumpur, Malaysia.
    MeSH terms: Adolescent; Adult; Asthma/drug therapy; Awareness; Bronchodilator Agents/administration & dosage*; Child; Equipment Design; Female; Humans; Male; Middle Aged; Nebulizers and Vaporizers*; Ozone*; Patient Acceptance of Health Care*; Chlorofluorocarbons*; Anti-Asthmatic Agents/administration & dosage
  9. Cheng HM, Chamley LW
    Proc. Soc. Exp. Biol. Med., 1998 Sep;218(4):277.
    PMID: 9714070
    MeSH terms: Glycoproteins/physiology*; Humans; Lipoproteins, LDL/metabolism*; Placenta/metabolism*; Trophoblasts/metabolism*; beta 2-Glycoprotein I
  10. Jin LZ, Ho YW, Abdullah N, Ali MA, Jalaludin S
    J Appl Microbiol, 1998 Jun;84(6):1171-4.
    PMID: 9717304
    Two Lactobacillus isolates, Lact. acidophilus I 26 and Lact. fermentum I 25, were selected, based on their poor aggregation with Escherichia coli and strong ability to adhere to ileal epithelial cells (IEC), to study in vitro interactions with E. coli O1:K1, O2:K1 and O78:K80 in an IEC radioactive-assay under the conditions of exclusion (lactobacilli and IEC, followed by the addition of E. coli), competition (lactobacilli, IEC and E. coli together) and displacement (E. coli and IEC, followed by the addition of lactobacilli). The results indicated that Lact. acidophilus I 26 and Lact. fermentum I 25 could not significantly reduce the attachment of E. coli O1:K1, O2:K1 and O78:K80 to IEC under the three conditions tested in vitro, except that the attachment of E. coli O1:K1 was slightly reduced by Lact. fermentum I 25 in the test for competition.
    MeSH terms: Animals; Bacterial Adhesion*; Chickens/microbiology; Epithelial Cells/microbiology; Escherichia coli/isolation & purification; Escherichia coli/physiology*; Ileum/microbiology; Intestinal Mucosa/microbiology*; Lactobacillus/physiology*; In Vitro Techniques
  11. Iyngkaran N, Yadav M
    J Trop Pediatr, 1998 08;44(4):199-203.
    PMID: 9718904 DOI: 10.1093/tropej/44.4.199
    Rice-starch based oral rehydration solution (ORS) has been shown to be a suitable alternative to glucose-based ORS in the treatment of both choleragenic and non-choleragenic dehydration in older infants and children. However, in young infants, the wider use of rice-starch ORS has been impeded because of theoretical concern about the poor digestibility of starch. The present study was conducted to evaluate the safety and efficacy of rice-starch ORS in the rehydration of acute diarrhoeal dehydration in infants below 6 months of age. Sixty-three infants with clinical features of acute gastroenteritis were randomly allocated to two groups. Group A, comprising 31 infants, received a rice-starch ORS and group B, comprising 32 infants, received a glucose-based ORS. The response to treatment was monitored by weight gain, stool frequency, and decrease in vomiting. The mean weight gain in moderately dehydrated and mildly dehydrated infants in both groups A and B were closely similar at 12, 24, and 48 h after treatment with the respective ORS solution. The infants without dehydration receiving rice-starch ORS had significantly greater weight gain at 12 h compared to those receiving glucose ORS. However, this difference was not observed at 24 and 48 h. The results of this study show that rice-starch ORS is as safe and efficacious as glucose-based ORS in young infants.
    MeSH terms: Dehydration/therapy*; Female; Fluid Therapy/methods; Glucose/administration & dosage*; Humans; Infant; Infant, Newborn; Male; Starch/administration & dosage*; Rehydration Solutions/administration & dosage*; Treatment Outcome
  12. Cannon CH, Peart DR, Leighton M
    Science, 1998 Aug 28;281(5381):1366-8.
    PMID: 9721105
    The effects of commercial logging on tree diversity in tropical rainforest are largely unknown. In this study, selectively logged tropical rainforest in Indonesian Borneo is shown to contain high tree species richness, despite severe structural damage. Plots logged 8 years before sampling contained fewer species of trees greater than 20 centimeters in diameter than did similar-sized unlogged plots. However, in samples of the same numbers of trees (requiring a 50 percent larger area), logged forest contained as many tree species as unlogged forest. These findings warrant reassessment of the conservation potential of large tracts of commercially logged tropical rainforest.
    MeSH terms: Borneo; Indonesia; Trees; Forestry; Forests; Rainforest
  13. Ong LC, Chandran V, Zasmani S, Lye MS
    J Paediatr Child Health, 1998 Aug;34(4):363-8.
    PMID: 9727180
    OBJECTIVES: To compare the neurobehavioural outcome of children aged 6-12 years with severe closed head injury [sCHI] (coma > 24 h), mild-to-moderate head injury [mCHI] (coma < 6 h) and orthopaedic controls.

    METHODS: Twenty-nine children in each group, matched for age, sex and ethnicity, were assessed using the Glasgow outcome Scale (GOS), Weschler Intelligence Scale for Children (WISC-III), Movement Assessment Battery for Children (Movement ABC), Wide Range Assessment of Learning and Memory (WRAML) and a standardised neurological examination 6 months post-injury. Parental reporting of pre- and post-injury behaviour was documented using the Child Behaviour Checklist (CBCL).

    RESULTS: Seven (24.1%) children with sCHI and three (10.3%) orthopaedic controls had residual motor deficits. Three (10.3%) children with sCHI and none in the other groups faced problems with independent ambulation. Twenty-seven (93.1%) of those with sCHI and all children in the other groups had GOS scores of good recovery or moderate disability. Twenty-two (81.5%) sCHI, five (18.5%) mCHI and one (3.7%) orthopaedic control reported a deterioration in school performance. MANOVAS identified a significant injury group effect for performance skills (P = 0.007), verbal skills (P = 0.002), memory and learning (P = 0.001) and motor skills (P = 0.001). Repeated measures ANOVA for pre- and post-injury CBCL scores showed significant differences related to somatic complaints (P = 0.004), problems of socialising (P = 0.003), delinquency (P = 0.004), aggressiveness (P = 0.010), thought (P < 0.001) and attention (P < 0.001). Post-hoc univariate analysis showed the significant differences were between that of the sCHI children and the other two groups.

    CONCLUSION: Although most sCHI children seemed to have made good physical recovery, there were cognitive, motor, memory and learning difficulties and behavioural problems concomitant with a deterioration in school performance compared with those with lesser or no head injury. This highlights the need for better integrated rehabilitation services to enable a gradual return into mainstream school.

    MeSH terms: Achievement; Analysis of Variance; Child; Child Behavior Disorders/etiology*; Child, Hospitalized/psychology; Cognition Disorders/etiology*; Female; Humans; Malaysia; Male; Movement Disorders/etiology; Neuropsychological Tests; Perceptual Disorders/etiology; Prospective Studies; Psychomotor Disorders/etiology; Glasgow Coma Scale; Chi-Square Distribution; Case-Control Studies; Head Injuries, Closed/complications*; Head Injuries, Closed/rehabilitation; Treatment Outcome; Cost of Illness
  14. Lee WS, Puthucheary SD, Boey CC
    J Paediatr Child Health, 1998 Aug;34(4):387-90.
    PMID: 9727185
    OBJECTIVE: To study the clinical features of non-typhoid Salmonella gastroenteritis and the incidence, risk factors and outcome of invasive complications in urban Malaysian children. To describe the serotypes of Salmonella species isolated and the pattern of antibiotic susceptibility.

    METHODOLOGY: Retrospective review of a group of 131 children with non-typhoid Salmonella gastroenteritis seen at the University Hospital, Kuala Lumpur, Malaysia from January 1994 to December 1996.

    RESULTS: Sixty-seven percent were infants below one year of age. Fever and vomiting were seen in nearly half of children. Seven children (5.3%) had invasive complications: 5 bacteraemia and 2 meningitis. Age below 6 months, fever > 38.0 degrees C, and dehydration on admission were significantly associated with invasive complications. The commonest serotypes isolated were S. enteritidis, S. paratyphi B, and S. bovis-morbificans. A total of 94-100% of isolates were susceptible to commonly prescribed antibiotics.

    CONCLUSIONS: Children with Salmonella gastroenteritis below 6 months of age who are febrile and dehydrated should be treated empirically with antibiotics until the result of blood culture is available.

    MeSH terms: Adolescent; Child; Child, Preschool; Drug Resistance, Microbial; Female; Gastroenteritis/complications; Gastroenteritis/drug therapy; Gastroenteritis/microbiology*; Gastroenteritis/epidemiology; Humans; Infant; Infant, Newborn; Malaysia/epidemiology; Male; Meningitis/microbiology; Meningitis/epidemiology; Microbial Sensitivity Tests; Retrospective Studies; Risk Factors; Salmonella/drug effects; Salmonella/isolation & purification; Salmonella Infections/complications; Salmonella Infections/drug therapy; Salmonella Infections/microbiology; Salmonella Infections/epidemiology*; Incidence; Bacteremia/microbiology; Bacteremia/epidemiology; Treatment Outcome
  15. Hisham AN, Samad SA, Sharifah NA
    Australas Radiol, 1998 Aug;42(3):250-1.
    PMID: 9727258
    Adrenal tumours are either functioning or non-functioning. Non-functioning adrenal tumours are generally asymptomatic and usually of enormous proportions at the time of presentation. A case is presented here of a patient with a huge right adrenal haemangioma which was successfully treated surgically. This unusual tumour was 25 cm in diameter, was well encapsulated and weighed 4 kg. The literature pertaining to this interesting case is reviewed.
    MeSH terms: Adrenal Gland Neoplasms/surgery; Female; Hemangioma/surgery; Humans; Middle Aged; Tomography, X-Ray Computed
  16. Gumel AB, Kubota K, Twizell EH
    Math Biosci, 1998 Aug 15;152(1):87-103.
    PMID: 9727298
    A sequential algorithm is developed for the non-linear dual-sorption model developed by Chandrasekaran et al. [1,2] which monitors pharmacokinetic profiles in percutaneous drug absorption. In the experimental study of percutaneous absorption, it is often observed that the lag-time decreases with the increase in the donor concentration when two or more donor concentrations of the same compound are used. The dual-sorption model has sometimes been employed to explain such experimental results. In this paper, it is shown that another feature observed after vehicle removal may also characterize the dual-sorption model. Soon after vehicle removal, the plots of the drug flux versus time become straight lines on a semilogarithmic scale as in the linear model, but the half-life is prolonged thereafter when the dual-sorption model prevails. The initial half-life after vehicle removal with a low donor concentration is longer than that with a higher donor concentration. These features, if observed in experiments, may be used as evidence to confirm that the dual-sorption model gives an explanation to the non-linear kinetic behaviour of a permeant.
    MeSH terms: Administration, Cutaneous; Algorithms*; Half-Life; Models, Biological*; Numerical Analysis, Computer-Assisted*; Pharmacokinetics; Skin Absorption/drug effects*; Linear Models
  17. Sankaran-Kutty M, Das PK, Kannan Kutty M
    Int Orthop, 1998;22(3):189-92.
    PMID: 9728315 DOI: 10.1007/s002640050239
    A comparative study of synovitis in Saudi Arabia and Malaysia was made with a view to determining any geographic variation in the incidence and pattern of the arthritides. The diagnostic spectrum in both series included pyogenic arthritis, rheumatoid arthritis, brucellar and tubercular arthritis, gout, pigmented villonodular synovitis, synovial chondromatosis and acute rheumatic fever. Date-palm thorn synovitis was observed only in the Saudi Arabian series. While brucellar and tuberculous arthritis were predominantly seen in Saudi Arabia, the incidence of rheumatoid arthritis, pigmented villonodular synovitis and acute suppurative arthritis was almost equal in both countries.
    MeSH terms: Adolescent; Adult; Aged; Biopsy; Child; Female; Humans; Malaysia/epidemiology; Male; Middle Aged; Saudi Arabia/epidemiology; Synovitis/etiology; Synovitis/epidemiology*; Synovitis/pathology; Incidence
  18. Liew WS, Leisner JJ, Rusul G, Radu S, Rassip A
    Int J Food Microbiol, 1998 Jul 21;42(3):167-73.
    PMID: 9728687
    The effect of heat-treatment on the internal temperature of raw cockles (Anadara granosa) and survival of their intrinsic flora of Vibrio spp. as well as of inoculated V. cholerae 0139 was examined. The cockles were purchased from markets in Malaysia and had an average weight including shells of 8.90+/-2.45 g. In one experiment heatpenetration of individual cockles was examined. Cockles weighing < 8 g (including shell) exhibited maximum internal temperatures of between 50 and 75 degrees C when heated in water at 99 degrees C for 10 s and 71-93 degrees C when heated for 30 s. Cockles weighing > 12 g exhibited maximum internal temperatures between 42 and 58 degrees C when heated in water at 99 degrees C for 10 s and 56-69 degrees C when heated for 30 s. In another experiment, heat-treatment of 10 cockles treated as a group at 99 degrees C for 10 or 30 s resulted in reduction of levels of intrinsic Vibrio spp. (enumerated directly on thiosulphate-citrate-bile salt sucrose agar; TCBS) from 5.73 to 3.15 log cfu g(-1) or below 1 log cfu g(-1), respectively. The levels of Vibrio spp. after heat-treatment decreased with an increase in numbers of cockles grouped together during treatment. In a third experiment V. cholerae 0139 was inoculated into cockles and subjected to heat-treatment at 99 degrees C for 0, 10, 15, 20, 25 or 30 s. The levels of Vibrio spp. in uninoculated, non-heat-treated cockles was 4.89 log cfu g(-1) on TCBS, and the predominant species were V. parahaemolyticus and V. alginolyticus. V. cholerae 0139 inoculated into cockles with an average weight of 13.5+/-1.90 g (including shell) decreased for samples examined immediately after heat-treatment from 6 log cfu g(-1) initially to 3.5 log cfu g(-1) after 25 s and < 1 log cfu g(-1) (TCBS) after 30 s of heat-treatment. The most probable number method by enrichment in alkaline peptone water gave in general within 1 log unit higher counts than TCBS direct enumeration. TCBS direct enumeration and MPN counts were up to 2.38 or 1.30 log units higher, respectively, for samples heat-treated for 20 s or longer and stored for 6 h at 30 degrees C before examination, than for samples heat-treated for same periods of time and examined immediately. This study shows that a mild heat-treatment of cockles for up to 25 s is inadequate to ensure a large reduction in numbers of Vibrio spp., including V. cholerae 0139.
    MeSH terms: Animals; Body Weight; Cholera/prevention & control*; Food Microbiology*; Hot Temperature; Humans; Hydrogen-Ion Concentration; Mollusca/microbiology*; Shellfish/microbiology*; Vibrio cholerae/growth & development*; Vibrio cholerae/physiology; Water Microbiology*; Colony Count, Microbial
  19. Zhao B, Lee EJ, Yeoh PN, Gong NH
    Pharmacogenetics, 1998 Aug;8(4):299-304.
    PMID: 9731716
    The xenobiotic metabolizing enzymes N-acetyltransferases (NATs) are important for the biotransformation and/or bioactivation of drugs and carcinogens. NATs are coded for in humans by two distinct genes, designated NAT1 and NAT2. NAT1, which was originally thought to be monomorphic, was recently reported to exhibit variation in human populations. Recent studies suggested that a genetic polymorphism of NAT1 may be associated with colorectal cancer risk. The distributions of NAT1 allele and genotype frequencies in unrelated individuals among Indian (n = 140), Malay (n = 122) and Chinese (n = 181) populations in Singapore were characterized by polymerase chain reaction-restriction fragment length polymorphism and allele-specific-polymerase chain reaction. The allelic frequencies of NAT1*3, NAT1*4, NAT1*10 and NAT1*11 among Indians were 0.3, 0.51, 0.17 and 0.02, respectively. The corresponding NAT1 allelic frequencies in Malays were 0.29, 0.30, 0.39 and 0.02, respectively, and were similar to those in Chinese in the region. The allelic frequencies of NAT1*3, NAT1*4, NAT1*10 and NAT1*11 among Chinese were 0.33, 0.35, 0.30 and 0.02, respectively. These findings are of importance for the determination of cancer risk in these populations. In addition, nucleotide changes at positions 350-351 (GG to CC) and 497-499 (GGG to CCC) of the NAT1 gene were not found in the alleles of the populations studied.
    MeSH terms: Acetyltransferases/genetics*; Adult; Aged; Alleles; Arylamine N-Acetyltransferase/genetics; Base Sequence; Female; Gene Frequency; Humans; India; Isoenzymes; Malaysia; Male; Middle Aged; Molecular Sequence Data; Mutation*; Polymorphism, Genetic*; Polymerase Chain Reaction; European Continental Ancestry Group/genetics; Asian Continental Ancestry Group/genetics
  20. Jin LZ, Ho YW, Abdullah N, Jalaludin S
    Poult Sci, 1998 Sep;77(9):1259-65.
    PMID: 9733111
    A study was conducted to determine the effects of adherent Lactobacillus culture on growth performance, intestinal microbial population, and serum cholesterol level of broilers. Four dietary treatments, consisting of the basal diet (control), basal diet + 0.05, 0.10, or 0.15% Lactobacillus culture (LC), were fed to 2,000 Arbor Acres broiler chicks from 1 to 42 d of age (DOA). The chicks were randomly assigned to 40 cages (50 chicks per cage, 10 cages per diet). The experimental period was 42 d. Body weights and feed to gain ratio were measured at 21 and 42 DOA. The intestinal microbial populations and serum cholesterol levels were determined at 10, 20, 30, and 40 DOA. The results showed that body weights and feed to gain ratios were improved significantly (P < 0.05) when compared to control broilers for broilers fed diets containing 0.05 or 0.10% LC, but not 0.15% LC, at 21 and 42 DOA. Coliform counts in the cecum of birds receiving 0.05% LC at 10, 20, and 30 DOA, and 0.10% at 10 and 20 DOA were significantly lower (P < 0.05) than those of the control birds. The total aerobes, total anaerobes, lactobacilli, and streptococci in the small intestines and ceca of the control birds were not significantly different from those of the treated groups. Serum cholesterol levels were significantly lower (P < 0.05) in broilers fed the three diets containing LC at 30 DOA, and in the birds fed 0.05 or 0.10% LC at 20 DOA.
    MeSH terms: Animals; Animal Nutritional Physiological Phenomena; Body Weight; Chickens/blood; Chickens/growth & development*; Chickens/microbiology; Cholesterol/blood*; Diet*; Intestines/microbiology*; Probiotics*
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