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  1. Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks
    Juanssilfero AB, Kahar P, Amza RL, Yopi, Sudesh K, Ogino C, et al.
    J Biosci Bioeng, 2019 Jun;127(6):726-731.
    PMID: 30642786 DOI: 10.1016/j.jbiosc.2018.12.002
    The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.
    Matched MeSH terms: Lipomyces/metabolism*
  2. Fulltext Growth kinetics of ethidium bromide mutagenized Lipomyces starkeyi strains
    Micky Vincent, Latifah Suali, Afizul Safwan Azahari, Patricia Rowena Mark Baran, Elexson Nillian, Lesley Maurice Bilung
    Journal of Advanced Research in Applied Sciences and Engineering Technology, 2018;11(1):47-54.
    MyJurnal
    Yeast growth and biomass production are greatly influenced by the length of the
    incubation period during cultivation. Therefore, this study was conducted to
    investigate the growth kinetics of five Lipomyces starkeyi strains as determined by
    biomass production. The five L. starkeyi strains, namely L. starkeyi ATCC 12659, L.
    starkeyi MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8, were inoculated
    in sterilized Yeast Malt broth, and, incubated for 192 hr at ambient temperature.
    Biomass yields were assessed and calculated gravimetrically every 24 hr. Results
    indicated that the optimal biomass production of L. starkeyi ATCC 12659, L. starkeyi
    MV-1, L. starkeyi MV-4, L. starkeyi MV-5 and L. starkeyi MV-8 were at 120, 168, 144,
    168 and 120 hr, with the concentrations of 6.64, 6.43, 9.78, 11.23 and 8.56 g/L,
    respectively. These results indicate that each L. starkeyi strain requires specific
    incubation period for the optimum production of fungal biomass. Therefore, by
    cultivating each L. starkeyi strain at the predetermined incubation period, biomass
    yields could significantly be improved for further downstream applications such as
    single cell protein and lipid production.
    Matched MeSH terms: Lipomyces
  3. Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation
    Karim A, Yousuf A, Islam MA, Naif YH, Faizal CKM, Alam MZ, et al.
    Biotechnol Prog, 2018 07;34(4):838-845.
    PMID: 29464927 DOI: 10.1002/btpr.2625
    The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m-3 ). At this condition, maximum lipid (63 mg g-1 ) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:838-845, 2018.
    Matched MeSH terms: Lipomyces/metabolism*
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