Displaying publications 21 - 40 of 205 in total

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  1. Connelly K, Kandane-Rathnayake R, Hoi A, Louthrenoo W, Hamijoyo L, Cho J, et al.
    Lancet Rheumatol, 2022 Dec;4(12):e831-e841.
    PMID: 38261391 DOI: 10.1016/S2665-9913(22)00307-1
    BACKGROUND: The selection and categorisation of laboratory tests in disease activity measures used within systemic lupus erythematosus (SLE) trial endpoints lack strong evidence. We aimed to determine whether longitudinal improvements in routinely measured laboratory tests are associated with measures of clinical improvement in patients with baseline active SLE.

    METHODS: We included patients from a multicentre longitudinal cohort (recruited between May 1, 2013, and Dec 31, 2019) with active SLE (SLEDAI-2K ≥6) coinciding with an abnormality in at least one of 13 routine laboratory tests, at a visit designated as baseline. At 12 months, we analysed associations between thresholds of improvement in individual laboratory test results, measured as continuous variables, and five clinical outcomes using logistic regression. Primary outcomes were damage accrual and lupus low disease activity state (LLDAS), and secondary outcomes were modified SLE responder index (mSRI), physician global assessment (PGA) improvement of at least 0·3, and flare.

    FINDINGS: We included 1525 patients (1415 [93%] women and 110 [7%] men, 1328 [87%] Asian ethnicity) in separate subsets for each laboratory test. The strongest associations with LLDAS and damage protection were seen with improvements in proteinuria (complete response: adjusted odds ratio [OR] 62·48, 95% CI 18·79-208·31 for LLDAS, OR 0·22, 95% CI 0·10-0·49 for damage accrual), albumin (complete response: adjusted OR 6·46, 95% CI 2·20-18·98 for LLDAS, OR 0·42, 95% CI 0·20-1·22 for damage accrual), haemoglobin (complete response: adjusted OR 1·97, 95% CI 1·09-3·53 for LLDAS, OR 0·33, 95% CI 0·15-0·71 for damage accrual), erythrocyte sedimentation rate (complete response: adjusted OR 1·71, 95% CI 1·10-2·67 for LLDAS, OR 0·53, 95% CI 0·30-0·94 for damage accrual), and platelets (complete response: adjusted OR 4·82, 95% CI 1·54-15·07 for LLDAS, OR 0·49, 95% CI 0·20-1·19 for damage accrual). Improvement in serological tests were mainly associated with PGA and mSRI. White cell and lymphocyte count improvements were least predictive.

    INTERPRETATION: Improvements in several routine laboratory tests correspond with clinical outcomes in SLE over 12 months. Tests with the strongest associations were discrepant with laboratory tests included in current trial endpoints, and associations were observed across a range of improvement thresholds including incomplete resolution. These findings suggest the need to revise the use of laboratory test results in SLE trial endpoints.

    FUNDING: Abbvie.

    Matched MeSH terms: Laboratories*
  2. Ajura AJ, Sumairi I, Lau SH
    Malays J Pathol, 2007 Dec;29(2):101-5.
    PMID: 19108402 MyJurnal
    Immunohistochemistry has become part of normal routine diagnostic work in the Stomatology Unit, Institute for Medical Research, Kuala Lumpur. Of 9523 cases received from the year 2000 to 2005, 197 cases (2.1%) required immunohistochemical staining. These cases ranged from benign to malignant lesions. They include lymphomas (n=41), epithelial tumours (n=29), neural lesions (n=21), fibroblastic/myofibroblastic tumours (n=16), small round cell tumour (n=11), vascular tumours (n=4), smooth muscle tumours (n=4), myxomatous tumours (n=4) and skeletal muscle tumours (n=1). In most of the cases (69.5%), immunohistochemical staining was mandatory to reach a definite diagnosis, while 60 cases (30.5%) required immunohistochemistry in confirming the diagnosis. In 32 cases (16.2%), definitive diagnosis could not be made due to the small size of the specimens received or the results of immunohistochemistry were inconclusive. Standardization of techniques, competent medical laboratory technologists and sufficient budget allocation are important in producing a high quality immunohistochemistry service.
    Matched MeSH terms: Laboratories/economics; Laboratories/standards*
  3. Hussain H, Khalid NM, Selamat R, Wan Nazaimoon WM
    Ann Lab Med, 2013 Sep;33(5):319-25.
    PMID: 24003421 DOI: 10.3343/alm.2013.33.5.319
    BACKGROUND: The urinary iodine micromethod (UIMM) is a modification of the conventional method and its performance needs evaluation.
    METHODS: UIMM performance was evaluated using the method validation and 2008 Iodine Deficiency Disorders survey data obtained from four urinary iodine (UI) laboratories. Method acceptability tests and Sigma quality metrics were determined using total allowable errors (TEas) set by two external quality assurance (EQA) providers.
    RESULTS: UIMM obeyed various method acceptability test criteria with some discrepancies at low concentrations. Method validation data calculated against the UI Quality Program (TUIQP) TEas showed that the Sigma metrics were at 2.75, 1.80, and 3.80 for 51±15.50 µg/L, 108±32.40 µg/L, and 149±38.60 µg/L UI, respectively. External quality control (EQC) data showed that the performance of the laboratories was within Sigma metrics of 0.85-1.12, 1.57-4.36, and 1.46-4.98 at 46.91±7.05 µg/L, 135.14±13.53 µg/L, and 238.58±17.90 µg/L, respectively. No laboratory showed a calculated total error (TEcalc)
    Matched MeSH terms: Laboratories/standards
  4. College of Pathologists, Academy of Medicine of, Malaysia
    Malays J Pathol, 2005 Jun;27(1):73-4.
    PMID: 16676699
    Matched MeSH terms: Laboratories/standards*
  5. Suppian R, Vegandraj S, Kandaiya S
    Int J Rad Appl Instrum A, 1992 Jul;43(7):937-8.
    PMID: 1321104
    Pumping air through a soft tissue which acts as a membrane is a relatively easy and quick method to collect and measure radon/thoron and its daughter nuclides in air. Analysis of the activity of the radionuclides can be calculated using an alpha counter which has been calibrated. In this method the activity of radon/thoron cannot be separated from the activity of radionuclides already present in the aerosol or dust particles.
    Matched MeSH terms: Laboratories*
  6. Lim VK, Cheong YM
    Malays J Pathol, 1992 Jun;14(1):41-3.
    PMID: 1469917
    A turnaround time study was conducted for bacteriological culture tests in seven Malaysian general hospitals. The turnaround times were determined using a specially designed form that was completed by the ward staff. Doctors at these hospitals were also polled to find out whether they were satisfied with the promptness of bacteriological test reporting in their hospitals. The turnaround times obtained from this survey were found to be satisfactory taking into account the constraints of laboratory methods employed. Nevertheless only about a third of doctors expressed satisfaction with the timeliness of the bacteriological test reporting. Doctors and microbiologists should get together and agree on acceptable standards of turnaround times that are practical and reasonable.
    Matched MeSH terms: Laboratories, Hospital/standards*
  7. Jegathesan M, de Witt GF
    Malays J Pathol, 1982 Aug;5:1-5.
    PMID: 7187452
    Matched MeSH terms: Laboratories/organization & administration*
  8. Fleming KA, Naidoo M, Wilson M, Flanigan J, Horton S, Kuti M, et al.
    Am J Clin Pathol, 2017 01 01;147(1):15-32.
    PMID: 28158414 DOI: 10.1093/ajcp/aqw143
    Objectives: We review the current status of pathology services in low- and middle-income countries and propose an “essential pathology package” along with estimated costs. The purpose is to provide guidance to policy makers as countries move toward universal health care systems.

    Methods: Five key themes were reviewed using existing literature (role of leadership; education, training, and continuing professional development; technology; accreditation, management, and quality standards; and reimbursement systems). A tiered system is described, building on existing proposals. The economic analysis draws on the very limited published studies, combined with expert opinion.

    Results: Countries have underinvested in pathology services, with detrimental effects on health care. The equipment needs for a tier 1 laboratory in a primary health facility are modest ($2-$5,000), compared with $150,000 to $200,000 in a district hospital, and higher in a referral hospital (depending on tests undertaken). Access to a national (or regional) specialized laboratory undertaking disease surveillance and registry is important. Recurrent costs of appropriate laboratories in district and referral hospitals are around 6% of the hospital budget in midsized hospitals and likely decline in the largest hospitals. Primary health facilities rely largely on single-use tests.

    Conclusions: Pathology is an essential component of good universal health care.

    Matched MeSH terms: Laboratories, Hospital/economics*
  9. Yap WH, Teoh ML, Tang YQ, Goh BH
    Biochem Mol Biol Educ, 2021 09;49(5):685-691.
    PMID: 34291546 DOI: 10.1002/bmb.21562
    This study presents an evaluation of integrating virtual laboratory simulations in assessment design of a biotechnology course at Taylor's University in Malaysia before, during and post-COVID recovery phases. The purpose was to investigate how virtual laboratory simulations were integrated as part of the assessments of a practical-embedded course-the aim being to evaluate students' acceptance and perception of using virtual simulation. A total of 46 students, across three different study cohorts (August 2019, March 2020, and August 2020) were evaluated different educational aspects of using virtual laboratory cases in a 4-week course within Animal Biotechnology. Overall, students regarded virtual laboratory simulation useful as part of their learning, and there is a significant increase in the level of acceptance before, during and post-COVID recovery phases. The study showed that across the different study cohorts, students perceived their confidence level in laboratory skills have been enhanced and that they can apply the skills in real-life situation. Interestingly, students (March and August 2020 cohort) who have not been exposed to the related laboratory session still perceived that the simulated activity provides clear explanation and realistic experience. Furthermore, it had been highlighted across the study cohorts that the quiz questions helped to enhance their understanding on the underlying principles of the laboratory techniques. The overall conclusion of this study was that structured simulation-based activities which provide clear instructions and explanation would support significant improvements in students learning.
    Matched MeSH terms: Laboratories*
  10. Mallhi TH, Khan AH, Sarriff A, Adnan AS, Khan YH, Jummaat F
    Postgrad Med J, 2016 Feb;92(1084):78-86.
    PMID: 26729887 DOI: 10.1136/postgradmedj-2015-133582
    Several criteria have been used to stratify acute kidney injury (AKI) in dengue infection and have resulted in variations in its incidence as well as clinic-laboratory characteristics. The current study was aimed to compare three commonly used criteria of AKI among patients with dengue.
    Matched MeSH terms: Laboratories
  11. Chua KH, Lee PC, Chai HC
    Malar J, 2016;15:134.
    PMID: 26931146 DOI: 10.1186/s12936-016-1183-z
    Detection of Plasmodium spp. is sometimes inconvenient especially in rural areas that are distant from a laboratory. In this study a portable diagnostic test of Plasmodium spp. was developed using insulated isothermal polymerase chain reaction (iiPCR) as an alternative approach to improve this situation.
    Matched MeSH terms: Laboratories
  12. Munksgaard NC, Kurita N, Sánchez-Murillo R, Ahmed N, Araguas L, Balachew DL, et al.
    Sci Rep, 2019 10 08;9(1):14419.
    PMID: 31595004 DOI: 10.1038/s41598-019-50973-9
    We present precipitation isotope data (δ2H and δ18O values) from 19 stations across the tropics collected from 2012 to 2017 under the Coordinated Research Project F31004 sponsored by the International Atomic Energy Agency. Rainfall samples were collected daily and analysed for stable isotopic ratios of oxygen and hydrogen by participating laboratories following a common analytical framework. We also calculated daily mean stratiform rainfall area fractions around each station over an area of 5° x 5° longitude/latitude based on TRMM/GPM satellite data. Isotope time series, along with information on rainfall amount and stratiform/convective proportions provide a valuable tool for rainfall characterisation and to improve the ability of isotope-enabled Global Circulation Models to predict variability and availability of inputs to fresh water resources across the tropics.
    Matched MeSH terms: Laboratories
  13. Chaudhry MH, Ahmad A, Gulzar Q, Farid MS, Shahabi H, Al-Ansari N
    Sensors (Basel), 2021 Feb 27;21(5).
    PMID: 33673425 DOI: 10.3390/s21051649
    Unmanned Aerial Vehicle (UAV) is one of the latest technologies for high spatial resolution 3D modeling of the Earth. The objectives of this study are to assess low-cost UAV data using image radiometric transformation techniques and investigate its effects on global and local accuracy of the Digital Surface Model (DSM). This research uses UAV Light Detection and Ranging (LIDAR) data from 80 meters and UAV Drone data from 300 and 500 meters flying height. RAW UAV images acquired from 500 meters flying height are radiometrically transformed in Matrix Laboratory (MATLAB). UAV images from 300 meters flying height are processed for the generation of 3D point cloud and DSM in Pix4D Mapper. UAV LIDAR data are used for the acquisition of Ground Control Points (GCP) and accuracy assessment of UAV Image data products. Accuracy of enhanced DSM with DSM generated from 300 meters flight height were analyzed for point cloud number, density and distribution. Root Mean Square Error (RMSE) value of Z is enhanced from ±2.15 meters to 0.11 meters. For local accuracy assessment of DSM, four different types of land covers are statistically compared with UAV LIDAR resulting in compatibility of enhancement technique with UAV LIDAR accuracy.
    Matched MeSH terms: Laboratories
  14. Song X, Hu Z, Shang L, Leaw CP, Lim PT, Tang YZ
    Harmful Algae, 2020 11;99:101926.
    PMID: 33218448 DOI: 10.1016/j.hal.2020.101926
    Multiple dinoflagellate species from the genus Karlodinium have been well known to form massive and toxic blooms that consequently cause fish kills in many coastal waters around the world. Karlodinium australe is a mixotrophic and potentially ichthyotoxic species associated with fish kills. Here, we investigated phagotrophy of K. australe (isolate KaJb05) established from a bloom event in the West Johor Strait, Malaysia, using several prey species (phytoplankton, zooplankton, and larval fish). The results showed that K. australe ingested relatively small prey cells of co-occurring microalgae by direct engulfment, while it fed on larger prey cells of microalgae by tube feeding. The results of animal exposure bioassays using rotifer (Brachionus plicatilis), brine shrimp (Artemia salina), and larval fish (Oryzias melastigma) demonstrated that phagotrophy (in terms of the trophic mode of the dinoflagellate), or micropredation (in terms of the mechanism of lethal effects on prey), played a more important role than the toxicity did in causing the lethal effects of K. australe on these aquatic animals under low cell densities of K. australe, while the mortalities of animals observed in the exposure to cell lysates of K. australe were solely caused by the toxicity. A comparison of the lethal effects between K. australe and K. veneficum revealed that the lethal effect of K. australe on rotifers was much stronger than that of K. veneficum at all cell densities applied in the experiments and the more "aggressive" micropredation of K. australe is suggested to explain the difference in lethal effect between K. austale and K. veneficum. Our results may explain why K. australe exhibited fish killings during moderate blooms at cell densities < 2.34 × 106 cells L-1, whereas K. veneficum was observed to cause massive fish kills only if the cell density was above 107 cells L-1. We believe these findings provide new insights into the ecological consequences of phagotrophy exhibited in some mixotrophic and harmful algae such as species of Karlodinium and of HAB events in general.
    Matched MeSH terms: Laboratories
  15. Hamidi H, Sharifi Haddad A, Wisdom Otumudia E, Rafati R, Mohammadian E, Azdarpour A, et al.
    Ultrasonics, 2021 Feb;110:106288.
    PMID: 33142226 DOI: 10.1016/j.ultras.2020.106288
    Ultrasound technique is an inexpensive and ecofriendly technology commonly used in oil and gas industry to improve oil recovery and its applications have been successfully tested in both laboratory and field scales. In this technique, high-power ultrasonic waves are utilized downhole to improve oil recovery and reduce formation damage in near wellbore region that causes a reduction in hydrocarbon production rate due to the penetration of mud, scale deposition, etc. In most of the cases, barriers for the oil flow to the wellbore are effectively removed by using the ultrasound technique and the effect of improved oil recovery may last up to several months. The aim of this paper is to provide an overview of recent laboratory, field and mathematical studies to serve as reference for future extensive examination of ultrasound assisted improved oil recovery. As an added value to this field of study, research gaps and opportunities based on the review of recent works were identified and factors that needs to be considered to improve the outcome of future studies were recommended.
    Matched MeSH terms: Laboratories
  16. Kingsbury AN
    Matched MeSH terms: Laboratories
  17. Hu CH, Ting R, Wong L
    Family Physician, 1991;3:70-73.
    Matched MeSH terms: Laboratories
  18. Ch'ng ES, Othman NH
    Malays J Pathol, 2021 Apr;43(1):19-23.
    PMID: 33903301
    International Academy of Pathology, Malaysian Division has initiated and run the external quality assurance program for general diagnostic histopathology since the year 2017. This article introduces the educational philosophy of this external quality assurance program and the technicalities in running such a national program. Challenges in ensuring the successful running of this program to gain wide acceptance by histopathology laboratories in Malaysia as well as experience in overcoming these challenges are detailed. This article charts the future direction of this external quality assurance program.
    Matched MeSH terms: Laboratories
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