A simple and sensitive fluorescence based biosensor for the determination of uric acid using H2O2-sensitive quantum dots/dual enzymes

Azmi NE 1 , Ramli NI 2 , Abdullah J 3 , Abdul Hamid MA 4 , Sidek H 2 , Abd Rahman S 5 Show all authors , Ariffin N 2 , Yusof NA 6

Affiliations 

  • 1 Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia
  • 2 Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia
  • 3 Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. Electronic address: [email protected]
  • 4 School of Applied Physics, Faculty of Sciences and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor D.E., Malaysia
  • 5 Industrial Biotechnology Research Centre, SIRIM Berhad, No.1, Persiaran Dato' Menteri, Section 2, P.O. Box 7035, 40700 Shah Alam, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia
  • 6 Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia
Biosens Bioelectron, 2015 May 15;67:129-33.
PMID: 25113659 DOI: 10.1016/j.bios.2014.07.056

Abstract

A novel optical detection system consisting of combination of uricase/HRP-CdS quantum dots (QDs) for the determination of uric acid in urine sample is described. The QDs was used as an indicator to reveal fluorescence property of the system resulting from enzymatic reaction of uricase and HRP (horseradish peroxidase), which is involved in oxidizing uric acid to allaintoin and hydrogen peroxide. The hydrogen peroxide produced was able to quench the QDs fluorescence, which was proportional to uric acid concentration. The system demonstrated sufficient activity of uricase and HRP at a ratio of 5U:5U and pH 7.0. The linearity of the system toward uric acid was in the concentration range of 125-1000 µM with detection limit of 125 µM.

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