Expression of ADR-α1, 2 and ADR-β2 in cumulus cell culture of infertile women with polycystic ovary syndrome and poor responder who are a candidate for IVF: the novel strategic role of clonidine in this expression

Zangeneh FZ 1 , Bagheri M 2 , Shoushtari MS 3 , Naghizadeh MM 4

Affiliations 

  • 1 Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
  • 2 Department of Reproductive Health, Faculty of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran
  • 3 Chemical and Environment Department, Faculty of Engineering, UPM, Selangor, Malaysia
  • 4 Nonproliferative Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
J Recept Signal Transduct Res, 2021 Jun;41(3):263-272.
PMID: 32878560 DOI: 10.1080/10799893.2020.1806320

Abstract

OBJECTIVE: Alpha and beta-adrenoceptors (ADR-α1, 2, and β2) play a regulatory role in the folliculogenesis and steroidogenesis in the ovarian follicles. This study aimed to measure these adrenoceptors mRNA and its protein levels in cumulus cells (CCs) culture of poor ovarian reserve (POR) and polycystic ovarian syndrome (PCOS) infertile women (IVF candidate) and the effect of clonidine treatment at CCs culture.

METHODS: This case/control study was conducted in 2017 includes a control (donation oocytes) and two studies (PCO and POR) groups. The ovulation induction drugs were prescribed in all groups. After the oocyte puncture, the follicular fluid was collected and CCs were isolated were cultured. RNA was extracted and cDNA was synthesized and designed the primer for the ADR-α1, 2 and ADR-β2 gene expression. The protein levels were investigated by Western Blot.

RESULTS: The results showed a high level of three adrenergic expressions in PCO women compared to the control group (p-value

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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