Affiliations 

  • 1 School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81300, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia
  • 2 School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia
  • 3 Health and Wellness Research Alliance, Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor Bahru, Malaysia
  • 4 School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81300, Skudai, Johor Bahru, Johor Darul Takzim, Malaysia; Health and Wellness Research Alliance, Universiti Teknologi Malaysia, 81310, UTM Skudai, Johor Bahru, Malaysia; Institute of Bioproduct Development, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia. Electronic address: [email protected]
Int J Biol Macromol, 2020 Sep 15;159:577-589.
PMID: 32380107 DOI: 10.1016/j.ijbiomac.2020.04.262

Abstract

Short-chain fructooligosaccharides (scFOSs) can be produced from the levan hydrolysis using levanase. Levanase from Bacillus lehensis G1 (rlevblg1) is an enzyme that specifically converts levan to scFOSs. However, the use of free levanase presents a lack of stability and reusability, thus hindering the synthesis of scFOSs for continuous reactions. Here, CLEAs for rlevblg1 were prepared and characterized. Cross-linked levanase aggregates using glutaraldehyde (CLLAs-ga) and bovine albumin serum (CLLAs-ga-bsa) showed the best activity recovery of 92.8% and 121.2%, respectively. The optimum temperature of CLLAs-ga and CLLAs-ga-bsa was increased to 35 °C and 40 °C, respectively, from its free rlevblg1 (30 °C). At high temperature (50 °C), the half-life of CLLAs-ga-bsa was higher than that of free rlevblg1 and CLLAs-ga. Both CLLAs exhibited higher stability at pH 9 and pH 10. Hyperactivation of CLLAs-ga-bsa was achieved with an effectiveness factor of more than 1 and with improved catalytic efficiency. After 3 h reaction, CLLAs-ga-bsa produced the highest total scFOSs yield of 35.4% and total sugar of 60.4% per gram levan. Finally, the reusability of CLLAs for 8 cycles with more than 50% activity retained makes them as a potential synthetic catalyst to be explored for scFOSs synthesis.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.