Dermatophytosis is a widespread disease produced by pathogenic fungus particularly Trichophyton rubrum. The present study reports on the formulation and evaluation of topical gel containing Allium sativum, Curcuma longa, Andrographis paniculata and Alpinia galanga extracts for significant antifungal potential. Minimum Inhibitory Concentration (MIC) of single plant and combination including synergistic effects were also determined. Macrodilution method was used to determine the MIC against Trichophyton rubrum. Gel formulations were evaluated using physicochemical parameters such as color, appearance, pH, viscosity, spreadability and stability testing. Overall MIC values of extracts against Trichophyton rubrum ranged from 31.25 mg/mL to 500 mg/mL. The MIC for A. sativum, C. longa, A. paniculata and A. galanga respectively were 500mg/ml, 31.25mg/ml, 250mg/ml and 500mg/mL. However, the assessment of combinations of selected extracts showed indifferent and antagonism interactions. This study also revealed that the formulation containing C. longa showed better stability than other formulations. A. galanga gel formulation has good viscosity and spreadability making it a good candidate in the gel formulation with good efficacy as an antifungal. Thus, this herbal gel formulation showed potential used to inhibit growth of T. rubrum that can be the alternative treatment of dermatophytosis.