Detection of virus-specific polymeric immunoglobulin A in acute hepatitis A, C, E virus serum samples using novel chimeric secretory component

Mohd Hanafiah K; Garcia ML; Barnes NC; Anderson DA

doi:10.1186/s13104-018-3799-2

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Detection of virus-specific polymeric immunoglobulin A in acute hepatitis A, C, E virus serum samples using novel chimeric secretory component

Mohd Hanafiah K ¹ , Garcia ML ² , Barnes NC ² , Anderson DA ²

Affiliations

¹ Life Sciences, Macfarlane Burnet Institute, 85 Commercial Rd, Melbourne, VIC, 3004, Australia. [email protected]
² Life Sciences, Macfarlane Burnet Institute, 85 Commercial Rd, Melbourne, VIC, 3004, Australia

BMC Res Notes, 2018 Oct 01;11(1):688.

PMID: 30285838 DOI: 10.1186/s13104-018-3799-2

Abstract

OBJECTIVE: To conduct a proof-of-concept study on preferential binding of polymeric IgA (pIgA) using a novel recombinant rabbit/human chimeric secretory component (cSC) and preliminary assessment of the diagnostic potential of virus-specific pIgA in discriminating acute hepatitis A, E, and C (HAV, HEV, HCV) patients and uninfected controls using an indirect enzyme-linked immunoassay.

RESULTS: cSC binds > 0.06 μg/ml of purified human and mouse pIgA with negligible cross-reactivity against IgM and IgA. Virus-specific pIgA was significantly higher in serum of acute HAV (n = 6) and HEV (n = 12) patients than uninfected samples (HEV: p

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.