Gene Silencing by RNAi in Mammalian Cells

Ponthan F 1 , Yusoff NM 2 , Soria NM 1 , Heidenreich O 1 , Coffey K 1

Affiliations 

  • 1 Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, United Kingdom
  • 2 Advanced Medical & Dental Institute, Universiti Sains Malaysia, Pulau Pinang, Malaysia
Curr Protoc Mol Biol, 2015 Jul 01;111:26.2.1-26.2.17.
PMID: 26131850 DOI: 10.1002/0471142727.mb2602s111

Abstract

This unit provides information how to use short interfering RNA (siRNA) for sequence-specific gene silencing in mammalian cells. Several methods for siRNA generation and optimization, as well as recommendations for cell transfection and transduction, are presented.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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