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Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods

Lin HR 1 , Heish CW 1 , Liu CH 1 , Muduli S 1 , Li HF 1 , Higuchi A 1 Show all authors , Kumar SS 2 , Alarfaj AA 3 , Munusamy MA 3 , Hsu ST 4 , Chen DC 5 , Benelli G 6 , Murugan K 7 , Cheng NC 8 , Wang HC 9 , Wu GJ 10

Affiliations 

  • 1 Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan
  • 2 Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
  • 3 Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia
  • 4 Department of Obstetrics and Gynecology, Taiwan Landseed Hospital, 77, Kuangtai Road, Pingjen City, Taoyuan 32405, Taiwan
  • 5 Department of Internal Medicine, Taiwan Landseed Hospital, 77, Kuangtai Road, Pingjen City, Taoyuan 32405, Taiwan
  • 6 Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy
  • 7 Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India
  • 8 Department of Surgery, National Taiwan University Hospital and College of Medicine, 7 Chung-Shan S. Rd., Taipei 100, Taiwan
  • 9 Hungchi Women &Children's Hospital, No. 233, Yuanhua Rd., Jhongli, Taoyuan 320, Taiwan
  • 10 Graduate Institute of Medical Sciences and Department of Obstetrics &Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan
Sci Rep, 2017 01 10;7:40069.
PMID: 28071738 DOI: 10.1038/srep40069

Abstract

Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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