Biopolymer collagen-chitosan scaffold containing Aloe vera for chondrogenic efficacy on cartilage tissue engineering

Jithendra P 1 , Mohamed JMM 2 , Annamalai D 3 , Al-Serwi RH 4 , Ibrahim AM 5 , El-Sherbiny M 6 Show all authors , Rajam AM 7 , Eldesoqui M 8 , Mansour N 9

Affiliations 

  • 1 Department of Biotechnology, CSIR-Central Leather Research Institute, Chennai 600020, India; Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Kuala Lumpur, Malaysia. Electronic address: [email protected]
  • 2 Vaasudhara College of Pharmacy, Sante Circle, Chintamani Road, Hoskote, 562114, Karnataka, India
  • 3 Centre for Academic and Research Excellence, CSIR-Central Leather Research Institute, Chennai 600020, India
  • 4 Department of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: [email protected]
  • 5 Department of Nursing, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia; Department of Family and Community Health Nursing, Faculty of Nursing, Port Said University, Egypt. Electronic address: [email protected]
  • 6 Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: [email protected]
  • 7 Medical Research center, Women's wellness Research Center, Hamad Medical Corporation, Doha, Qatar
  • 8 Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia; Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: [email protected]
  • 9 Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia. Electronic address: [email protected]
Int J Biol Macromol, 2023 Sep 01;248:125948.
PMID: 37482169 DOI: 10.1016/j.ijbiomac.2023.125948

Abstract

The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes in a standard condition. Cytocompatibility was analyzed using fluorescent dyes (calcein AM/ethidium bromide) and the viable cells were quantified by MTT assay. Glycosaminoglycan (GAG) content of ECM was estimated by using 1, 9-Dimethyl methylene Blue (DMMB). The total RNA content was quantified and the cartilage specific genes (col2a1, Acan) were amplified by reverse transcription-PCR from the cell lysate of the scaffolds. Histological examination was made using Haematoxylin and Eosin (H&E), safranin-O, masson's trichrome, alcian blue, and alizarin red to stain the specific component of ECM secreted on the construct. The cartilage specific collagen type II was estimated by immunohistochemistry using monoclonal type II collagen antibody. The results of these studies proved that COL-CS-AV scaffold has more chondrogenic efficacy than COL-CS, thus the aloe vera blend COL-CS-AV scaffold might be used as suitable candidate for cartilage tissue engineering.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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