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Effect of Collagen and GelMA on Preservation of the Costal Chondrocytes' Phenotype in a Scaffold in vivo

Isaeva EV 1 , Kisel AA 2 , Beketov EE 3 , Demyashkin GA 4 , Yakovleva ND 5 , Lagoda TS 6 Show all authors , Arguchinskaya NV 7 , Baranovsky DS 8 , Ivanov SA 9 , Shegay PV 10 , Kaprin AD 11

Affiliations 

  • 1 Senior Researcher, Laboratory of Tissue Engineering; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia
  • 2 Researcher, Laboratory of Tissue Engineering; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia
  • 3 Researcher, Laboratory of Medical and Environmental Dosimetry and Radiation Safety; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia; Associate Professor, Engineering Physics Institute of Biomedicine; Obninsk Institute for Nuclear Power Engineering - Branch of the National Research Nuclear University MEPhI, 1 Studgorodok, Obninsk, 249034, Russia
  • 4 Head of the Department of Pathomorphology; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia; Head of Department of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology; I.M. Sechenov First Moscow State Medical University (Sechenov University), 8/2 Malaya Trubetskaya St., Moscow, 119991, Russia
  • 5 Lecturer; Medical Technical School, 75 A Lenina St., Obninsk, 249037, Russia
  • 6 Research Laboratory Assistant, Laboratory of Tissue Engineering; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia
  • 7 Junior Researcher, Laboratory of Tissue Engineering; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia
  • 8 Head of Laboratory of Tissue Engineering; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia; Researcher, Research and Educational Resource Center for Cellular Technologies; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia
  • 9 Corresponding Member of the Russian Academy of Sciences, Director; A. Tsyb Medical Radiological Research Centre - Branch of the National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation, 10 Zhukova St., Obninsk, 249036, Russia; Professor, Department of Oncology and X-ray Radiology named after V.P. Kharchenko, Medical Institute; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia
  • 10 Head of the Center for Innovative Radiological and Regenerative Technologies; National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroleva St., Obninsk, 249036, Russia
  • 11 Professor, Academician of the Russian Academy of Sciences, General Director; National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, 4 Koroleva St., Obninsk, 249036, Russia Head of the Department of Urology and Operative Nephrology with a Course of Oncourology, Medical Institute; Peoples' Friendship University of Russia, 6 Miklukho-Maklaya St., Moscow, 117198, Russia
Sovrem Tekhnologii Med, 2023;15(2):5-16.
PMID: 37389022 DOI: 10.17691/stm2023.15.2.01

Abstract

The aim of the study was to compare type I collagen-based and methacryloyl gelatin-based (GelMA) hydrogels by their ability to form hyaline cartilage in animals after subcutaneous implantation of scaffolds.

MATERIALS AND METHODS: Chondrocytes were isolated from the costal cartilage of newborn rats using 0.15% collagenase solution in DMEM. The cells was characterized by glycosaminoglycan staining with alcian blue. Chondrocyte scaffolds were obtained from 4% type I porcine atelocollagen and 10% GelMA by micromolding and then implanted subcutaneously into the withers of two groups of Wistar rats. Histological and immunohistochemical studies were performed on days 12 and 26 after implantation. Tissue samples were stained with hematoxylin and eosin, alcian blue; type I and type II collagens were identified by the corresponding antibodies.

RESULTS: The implanted scaffolds induced a moderate inflammatory response in both groups when implanted in animals. By day 26 after implantation, both collagen and GelMA had almost completely resorbed. Cartilage tissue formation was observed in both animal groups. The newly formed tissue was stained intensively with alcian blue, and the cells were positive for both types of collagen. Cartilage tissue was formed among muscle fibers.

CONCLUSION: The ability of collagen type I and GelMA hydrogels to form hyaline cartilage in animals after subcutaneous implantation of scaffolds was studied. Both collagen and GelMA contributed to formation of hyaline-like cartilage tissue type in animals, but the chondrocyte phenotype is characterized as mixed. Additional detailed studies of possible mechanisms of chondrogenesis under the influence of each of the hydrogels are needed.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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