Knema retusa is antibacterial and antibiofilm against antibiotic resistant Staphylococcus aureus and S. haemolyticus isolated in bovine mastitis

Chuprom J 1 , Kidsin K 2 , Sangkanu S 3 , Nissapatorn V 3 , Wiart C 4 , de Lourdes Pereira M 5 Show all authors , Wongtawan T 1 , Daus M 6 , Sotthibandhu DS 7 , Tipmanee V 8 , Paul AK 9 , Scholfield CN 1 , Zulkipli MB 10 , Abdullah NH 11 , Mitsuwan W 12

Affiliations 

  • 1 Akkhraratchakumari Veterinary College, Walailak University, 80160, Nakhon Si Thammarat, Thailand
  • 2 Animal Health Section, Department of Livestock Development, The Eight Reginal Livestock Office, 84000, Surat Thani, Thailand
  • 3 School of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), and Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, 80160, Nakhon Si Thammarat, Thailand
  • 4 The Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Jalan UMS, Kota Kin-abalu, 88400, Sabah, Malaysia
  • 5 Department of Medical Sciences, CICECO-Aveiro Institute of Materials, University of Aveiro, Aveiro, Portugal
  • 6 Division of Physical Science and Natural Product Center of Excellent, Faculty of Science, Prince of Songkla University, 80160, Songkhla, Thailand
  • 7 Faculty of Medical Technology, Prince of Songkla University, 90110, Songkhla, Thailand
  • 8 Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, 90110, Songkhla, Thailand
  • 9 School of Pharmacy and Pharmacology, University of Tasmania, 7001, Hobart, TAS, Australia
  • 10 School of Pharmacy, University of Nottingham Malaysia Campus, 43500, Semenyih, Malaysia
  • 11 Natural Product Division, Forest Research Institute Malaysia (FRIM), 52109, Kepong, Malaysia
  • 12 Akkhraratchakumari Veterinary College, Walailak University, 80160, Nakhon Si Thammarat, Thailand. [email protected]
Vet Res Commun, 2023 Jun;47(2):523-538.
PMID: 36260188 DOI: 10.1007/s11259-022-09999-0

Abstract

This study aimed to assess antibacterial activity of Knema retusa wood extract (KRe) against antibiotic resistant staphylococci which are causative agents of bovine mastitis. From 75 cases of intramammary infections in dairy cows, 66 staphylococcal isolates were collected, including 11 Staphylococcus aureus isolates (17%) and 55 coagulase-negative staphylococci (83%). Sixty isolates (91%) formed strong biofilms. KRe had minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) against the isolates ranging 32-256 ug/mL and 64-512 ug/mL, respectively. Two-hour KRe exposures at 4×MIC, viabilities of S. aureus and S. haemolyticus decreased by 3 log10 compared to the control. Scanning EM (SEM) showed that KRe disrupted the bacterial cells of both species. KRe at 1/16×MIC significantly inhibited biofilm formation (P 

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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