Fulltext

Sea grapes powder with the addition of tempe rich in collagen: An anti-aging functional food

Permatasari HK 1 , Nurkolis F 2 , Vivo CD 3 , Noor SL 4 , Rahmawati R 5 , Radu S 6 Show all authors , Hardinsyah H 7 , Taslim NA 8 , Mayulu N 9 , Wewengkang DS 10 , Kuswari M 11 , Batubara SC 5 , Gunawan WB 12 , Nahdi MS 2

Affiliations 

  • 1 Department of Biochemistry and Biomolecular, Faculty of Medicine, Brawijaya University, Malang, 65145, Indonesia
  • 2 Department of Biological Sciences, Faculty of Sciences and Technology, State Islamic University of Sunan Kalijaga, Yogyakarta, 55281, Indonesia
  • 3 Dentistry, University of Indonesia, Depok, 16424, Indonesia
  • 4 Clinical and Public Health Nutrition Programme, University College London, London, WC1E 6BT, UK
  • 5 Food Technology Department, Sahid University of Jakarta, South Jakarta, 12870, Indonesia
  • 6 Food Sciences, Faculty of Human Ecology, Universiti Putra Malaysia, Serdang Selangor Darul Ehsan, 43400, Malaysia
  • 7 Applied Nutrition, Faculty of Human Ecology, IPB University, Bogor, 16680, Indonesia
  • 8 Clinical Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia
  • 9 Nutrition and Food, Faculty of Medicine, Sam Ratulangi University, Manado, 95115, Indonesia
  • 10 Pharmacy department, Faculty of Mathematics and Sciences, Sam Ratulangi University, Manado, 95115, Indonesia
  • 11 Nutrition Department, Faculty of health sciences, Universitas Esa Unggul, Jakarta, 11510, Indonesia
  • 12 Nutrition Sciences Department, Faculty of Medicine, Diponegoro University, Semarang, 50275, Indonesia
F1000Res, 2021;10:789.
PMID: 36237995 DOI: 10.12688/f1000research.55307.3

Abstract

Background: This study aimed to determine the potential anti-aging effects of sea grapes and tempe (fermented soybeans) collagen particle size, by measuring the activities of anti-glycation, antioxidant, and tyrosinase inhibitors. Methods: Collagen was isolated from freeze-dried sea grapes and tempe powder and treated with different NaOH concentrations (0.10 M; 0.20 M; 0.30 M), and CH 3COOH 1 M solution, separately. The collagen particle size was adjusted by stirring at 1000 rpm for 5 and 10 hours. 2,2-diphenyl-1-picrylhydrazyl (DPPH) was used to measure the antioxidant activity, and L-tyrosine and L-DOPA (l-3,4-dihydroxyphenylalanine) was used as a marker of tyrosine inhibition.  Results:  The collagen treated with 0.10 M NaOH produced the highest collagen yield (11.65%), and the largest particle size (2455 nm). Additionally, this collagen, when treated for 5 hours, exhibited 24.70% antioxidant activity, 62.60% anti-glycation, 8.97% L-tyrosine, and 26.77% L-Dopa inhibition activities. Meanwhile, the collagen treated for 10 hours had a 9.98% antioxidant activity, 41.48% anti-glycation, 7.89% L-tyrosine, and 2.67% L-Dopa inhibition activity.  Conclusion: Sea grapes and tempe collagen powder treated with 0.10 M NaOH and stirred for 5 hours, possess the best potential anti-aging properties as a functional food.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

MeSH terms
Similar publications