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Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Moreno I 1 , Garcia-Grau I 1 , Perez-Villaroya D 2 , Gonzalez-Monfort M 1 , Bahçeci M 3 , Barrionuevo MJ 4 Show all authors , Taguchi S 5 , Puente E 6 , Dimattina M 7 , Lim MW 8 , Meneghini G 9 , Aubuchon M 10 , Leondires M 11 , Izquierdo A 12 , Perez-Olgiati M 13 , Chavez A 14 , Seethram K 15 , Bau D 2 , Gomez C 2 , Valbuena D 2 , Vilella F 1 , Simon C 16

Affiliations 

  • 1 Igenomix Foundation, INCLIVA Health Research Institute, Valencia, Spain
  • 2 Igenomix R&D, Valencia, Spain
  • 3 Bahçeci Group, Istanbul, Turkey
  • 4 IVF Florida, Margate, FL, USA
  • 5 Oak Clinic Sumiyoshi, Osaka, Osaka Prefecture, Japan
  • 6 Clinica Fertia, Fuengirola, Malaga, Spain
  • 7 Dominion Fertility, Arlington, WA, USA
  • 8 Alpha IVF and Women's Specialists Centre, Petaling Jaya, Selangor, Malaysia
  • 9 Gestanza Medicina Reproductiva, Rosario, Santa Fe, Argentina
  • 10 Missouri Center for Reproductive Medicine, Chesterfield, MO, USA
  • 11 RMA Connecticut, Norwalk, CT, USA
  • 12 ProcreaTec, Madrid, Spain
  • 13 Pregna Medicina Reproductiva, Buenos Aires, Argentina
  • 14 New Hope Fertility Center, Mexico City, Mexico
  • 15 Pacific Centre for Reproductive Medicine, Burnaby, British Columbia, Canada
  • 16 Igenomix Foundation, INCLIVA Health Research Institute, Valencia, Spain. [email protected]
Microbiome, 2022 Jan 04;10(1):1.
PMID: 34980280 DOI: 10.1186/s40168-021-01184-w

Abstract

BACKGROUND: Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage or no pregnancy.

METHODS: Here, we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments.

RESULTS: A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes.

CONCLUSIONS: Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies. Video Abstract.

* Title and MeSH Headings from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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