A study on distribution and diversity of benthic foraminifera in surface sediments was carried out in Northwestern Sarawak waters, Malaysia. The range of water depth at the study site was between 43 m and 71 m. A total of seven sediment samples were taken for this study. As a result, 11 genera were identified from a total of 1,222 individuals of foraminifera. 200 individuals were picked out from each sample. The 11 genera that were identified from the study site included Heterolepaspp., Textulariaspp., Quinqueloculinaspp.,Operculina spp. Pseudorotaliaspp., Amphisteginaspp., Cylindroclavulinaspp., Elphidiumspp., Ammobaculitesspp., Asterorotaliaspp. and Bolivinaspp.. The common genera found in the sediments of the study areas were Heterolepa, Textularia, Quincoloculina, Operculinaand Pseudorotalia. The highest and lowest values of Fisher alpha and Shannon-Wiener indices were shown at Station C287 and Station B482 respectively. The highest value of Fisher alpha was 3.23 and the lowest value was 1.97. The highest and lowest values of Shannon-Wiener were 2.30 and 1.91. The highest index value of diversity was 3.23 at depth 67.76 m and the lowest value was 1.53 at depth 45.54 m and 68.45 m. From this study, depth is not the main factor that influencesthe diversity of benthic foraminiferal in northwestern Sarawak waters.
Biopolymer interaction in oil-in-water (o/w) emulsions has been demonstrated to positively modify the emulsion physicochemical properties which lead to desirable stability. The present work focused on the effect of pea protein isolate (PPI), pectin, carboxymethyl cellulose (CMC) and their interaction on physicochemical properties and oxidative stability of o/w emulsions using a mixture design approach. The emulsions were prepared with 40 % sunflower oil stabilized with 1 % of PPI, pectin and CMC, respectively, as well as their mixtures according to a simplex-centroid design (10 points). ThepH values for all emulsions were within acidic condition (3.22 to 4.66) and increased significantly (p
Vinegars are most widely used as preservatives in food industry. Vinegars are known for their health benefits; however, the roles of vinegar-associated microflora in locally produced vinegars are not well established. The objectives of this study are to isolate and identify the lactic acid bacteria (LAB) from black rice vinegar and coconut vinegar, measure their pH and titratable acidity, and determine their antibacterial activity. LAB was isolated using cultural method. Phenotypic characterization of LAB was carried out using Gram-staining, oxidase test, catalase test and API 50 CHL Kit. Results from API 50 CHL Kit confirmed that BRV03M strain from black rice vinegar and CV03M strain from coconut vinegar were Lactobacillus paracaseissp. paracasei. The identified bacteria in both samples were consistent as L. paracaseiusing 16S rDNAgene sequences with 93% and 99% similarity, respectively. The pH and titratable acidity percentage of both vinegars were also determined. The stability of Cell Free Supernatant-Lactic Acid Bacteria (CFS-LAB) strains within 14 days on their inhibition against selected pathogenic bacteria was determined using agar well diffusion method. The CFS-LAB strain isolated from black rice vinegar (BRV03M) was more stable within 14 days than coconut vinegar in inhibiting tested bacteria, suggesting this strain has great potential as natural antibacterial agents.
Nowadays, Kenaf fiber is sustainably useful in marine structures and has become one of the materials that may be high in demand as it is light, biodegradable and environmental friendly. This study investigates the effect of fiber percentage on compressive strength of fiber reinforced concrete (FRC) and the relationship between compressive strength and time of FRC immersion in seawater. FRC concrete cubes were prepared using four different percentage of fiber (0%, 1.5%, 3.0% and 4.5%). These FRC were immersed in seawater for 7, 14 and 21 days for three consecutive weeks. Based on the experiment, it was found that there was improvement in compressive strength of FRC when compared to plain cement concrete. The results showedthat 3.0% of KF to cement matrix concrete determined the highest compressive strength of 205.43 Pa while 0% of KF fiber to cement concrete matrix (control specimen) showed the lowest compressive strength of 158.28 Pa. Also the addition of Kenaf fiber to cement concrete decreased the seawater absorption more than concrete with absolutely 0% of KF fiber to cement concrete (control specimen). In conclusion, the results did show significant improvement and a consistent trend on strength with the addition of FRC. This study also revealed that the percentage of water absorption was on the increase for 0, 7 and 14 days and become constant after day 21. This is due to manufacturing defects that occurred which block the water from entering the material and making the material absorb less water.
MeSH terms: Bone Cements; Construction Materials; Dental Materials; Glass Ionomer Cements; Immersion; Seawater; Water; Compressive Strength; Hibiscus
At present, heavy metal pollution is a major environmental concern and the adsorption technique is a potent method for removal of these heavy metals from wastewater. Activated carbon is one of the best adsorbents for metal ionsremoval but it is sometimes restricted due to high cost and problems with regeneration hamper large scale application. Low cost adsorbent is alternatively being introduced to replace activated carbon since it is available in large quantity, renewable and inexpensive. Hence, Pennisetum purpureum(elephant grass) was investigated for its potential in cadmium ions removal. The adsorbent was characterized by Fourier Transforms Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda (BJH) analyses.The effects of pH (1 to 5), initial metal ion concentration (5 to 25 mg/L), contact time (10 to 60 minutes) and adsorbent dosage (0.2 to 1.0 g) on cadmium ions removal were conducted by batch adsorption experiments. In this study, the FT-IR results demonstrated that the functional groups for untreated and nitric acid-treated P. purpureum mainly consisted of carbonyl, carboxyl, hydroxyl and amine groups which are able to bind with positively charged cadmium ions. SEM micrographs have proven that nitric acid modification would remove the surface impurities of P. purpureum, which increased the surface roughness, produced deep, open pores and better pore size distribution. From the BET and BJH analyses, the treated P. purpureum was mesoporous, had larger surface area and pore volume compared to untreated P. purpureum. The best pH, adsorbent dosage and contact time were pH 4, 0.6 g and 30 minutes, respectively. The highest removal percentage of cadmium ions for both untreated and treated P. purpureum were 92% and 98% correspondingly. The results shown strengthened the fact that both biosorbents have great potential in cadmium ions removal.
Solar photocatalysis is a green technology that takes advantage of sustainable solar energy for enhancing oxidation process of numerous harmful water contaminants. In this study, a custom solar driven zinc oxide (ZnO)-mediated photocatalytic system was developed and its efficiency to remove organic contaminants as well as to disinfect selected bacteria was investigated. Methylene blue (MB) dye was used as the model organic contaminant, while Escherichia coli(E.coli) was used as the model fecal coliform bacteria in contaminated water. A series of photodegradation experiments were conducted on water contaminated with either 10 mg/L of MB or ~1010CFU/ml of E.coli. The experiments were completed under sunlight irradiation in the presence of 1 g/L of nano ZnO photocatalyst for up to 6 hours. Using a solar thermal collector, the photoreactor operated in the temperature range of 25 to 50 oC. The findings revealed that the combination of solar thermal with solar photocatalysis usingZnO intensified the degradation of MB and disinfection of E.coli. 98.08% of MB dye and 99.99% of E.coliwere successfully removed from the water within the first 3 hours of treatment. Almost complete removal was eventually achieved after 6 hours of treatment. It is therefore suggested that ZnO-based solar photocatalytic system developed in this study is highly efficient at enhancing water decontamination process.
Pink Antigonon leptopushave potential to be commercialized as cut flowers for flower arrangement. In order to determine cut inflorescences' vase life, vase solution treatments containing Artificial Tap Water as control, salicyclic acid (SA) at 100, 200, 300 mg/L and combination of 100, 200, 300 mg/L SA with 2% sucrose were conducted. Parameters observed were vase life, relative fresh weight (RFW), vase solution uptake (VSU), flower drop (FD), flower colour, relative water content (RWC) and pH. The results showed that cut inflorescences in vase treatment containing 200 mg/L SA + 2% sucrose and 300 mg/L + 2% sucrose had 1.6 fold longer vase life than the control, showing higher water uptake and reduced flower drop by 28%.
Pineapple is one of the most important commercial fruit crops served in fresh-cut form which is convenientfor the consumers. However, fresh-cut pineapple induces the activity of phenolic compounds which triggers the generation of brown or dark pigments. Browning incidence (BI) directly influences the fruit’s acceptability and marketability. Therefore, different exposure times (5mins, 10mins, 15mins and 20mins)and typesof LED lights (white, red and blue) were applied on fresh-cut pineapple stored at 5oC storage for twelve days to reduce BI. A significant interaction between the two factors was recorded in lightness coefficient, chroma, total phenolic and ascorbic acid (AA)contents. Regardless of exposure times, all types of LED lights, mainly the blue light, succeeded in delaying BI in fresh-cut pineapple. In conclusion, blue light had a tendency todelay BI andmaintaintheother postharvest quality attributes of fresh-cut pineapple.
The emergence of mega vessels is not a new event in container shipping industry. Containerization has caused a spectacular growth in maritime transportation and brought significant developments to the industry, ranging from operation systems to seaport infrastructure expansion. Container vessels have gotten larger, with some having the capacity of 18,000 TEUs (Triple E Class), to cope with the demand for economies of scale (EOS). Mega vessels can influence the strategic planning of seaport operators for massive infrastructure development and investment on existing terminals at the seaports. The objective ofthis research is to identify and measure the requirements for seaport expansion that need to be prioritized by seaport operators to accommodate mega vessels. Additionally, this research aims to determine the challenges faced by seaport operator to meet the seaport infrastructure expansion requirements. Two mathematical methods were employed, namely Analytical Hierarchy Process (AHP) and Evidential Reasoning (ER). The results showed that the most important requirement for seaport expansion is navigation aspect followed by berth expansion, terminal, yard and gate operations. This research is expected to assist seaport operator in making a decision on prioritizing t requirements for expansion. Furthermore, this research aims to measure the readiness of seaports to serve mega vessels in global shipping industry in order to achieve competitive advantages.
This study was carried out in Marang, Terengganu. The objective of this study is to analyse the socio-economicprofileof fishermen and their monthly income. The Slovin’s formula was used to calculate thenumber ofrespondentsin Marang and the data was collected through a survey involving 50 respondents consisting of Malay fishermen. The results of the study showedthat the dominant types of fishing gear used were hooks and lines, gillnets and purse seines. The socio-economic profile showedthat a majority of the fishermen (35%) were aged between 40-49years, 36% of the fishermen had between 10-19 yearsof experience, and 55% of the fishermen hadbetween 2-5 children. The average monthly income of the fishermen was MYR1853.49 for those using hooks and lines, MYR2105.09 for gillnets and MYR2595.39 for purse seines. It appears that the use of purse seinesgenerated the highest income for the fishermen. Generally, the income was not fixed as it depended on the amount of catch for each fisherman’s trip, which was also affected by the season.
DNA Barcoding, primarily focusing on cytochrome coxidase subunit I (COI) gene has been appraised as an effective tool for species identification. Nonetheless, species identification based on molecular approach is essential for discrimination of look-alike species. In this study, we focused on the marine fishes Family Nemipteridae, one of the commercially important group distributed within the surrounding seas of Malaysia. Some of the samples were collected during the National Demersal Trawl Survey in the Exclusive Economic Zone of East Coast Peninsular Malaysia by the Department of Fishery Malaysia. A 652bp region of COI was sequenced for 74 individuals from nine putative species. Additional 34 COIsequences from GenBank were also included in this study making the total number of samples analysed to 108 individuals. The averageKimura 2-parameter (K2P) nucleotide divergence was 0.34% among individuals within species and 6.97% within genera. All putative species formed monophyletic clades in both Neighbour-joining (NJ) and Maximum-likelihood (ML) trees. However, there was a potential misidentification in specimen identified as Nemipterus tambuloides,as the specimen did not group with their own taxa. It was genetically grouped in Nemipterus thosaporni clade. This study supports the effectiveness of COIgene in species discrimination of Family Nemipteridae.
MeSH terms: Animals; Base Sequence; Cytochromes; DNA; Fisheries; Fishes; Humans; Malaysia; Nucleotides; Oceans and Seas; Surveys and Questionnaires; Trees; Databases, Nucleic Acid; DNA Barcoding, Taxonomic
This study was conducted to determine the morphology, prevalence and mean intensity of monogeneans on gill filaments of striped catfish Pangasianodon hypophthalmus (Pangasiidae).P. hypophthalmus are important economic fish in Southeast Asia region and predominantly produced in Asian countries such as Malaysia, Bangladesh, Indonesia, Vietnam, Laos, China and Cambodia through aquaculture. Thirty fish were sampled from an earthen pond located in Kuala Besut, Terengganu, in the period from January to February, 2018. The monogenean parasites were removed from the gill filaments and counted. For the morphological study, the extracted monogeneanswere mounted on a slide by using drop of ammonium picrate-glycerin (APG) and then were observed under the Compound Advanced Research Microscope. The drawing was done with the aid of lucida camera attached to compound microscope. The monogenean parasite was identified as Thaparocleidussp based on the characteristics of the morphology and morphometrics of the parasite, which did not differ significantly from the previous descriptions of the same species discovered in other geographic locations. The prevalence and mean intensity levels were 100% and 106.07 parasites per fish, respectively. The monogenean parasites discovered throughout this examination were recorded and the data obtained was summarized.
The number of small and medium-sized accommodations (SMSAs) has increased in Malaysia in tandem with the growth of the tourism industry worldwide. The states involved in this study were Terengganu, Kelantan and Pahang. The SMSAs in these states are rated using Orchid rating, namely 1-Orchid, 2-Orchid and 3-Orchid, generated by the Ministry of Tourism, Arts and Culture Malaysia (MOTAC). This study applied the Resource-based View (RBV) theory to investigate four factors (marketing management, human resourcesmanagement, innovation capability management and information technology) assumed to be competitive capabilities that may influence accommodation’s performance. The objective of the study is to examine whether the four factors influence the performance of Orchid-rated accommodations located in the East Coast of West Malaysia. The results revealed that the four factors do not have any relationship with the accommodation’s performance. The analysis also singled out one argument of the director of SMSA that competitive capabilities are not important to their business. This argument coincides with the results of the analysis that the four factors are not significant with regards to the accommodations’ performance.
Plastics packaging is non-biodegradable and risks to human health and environmental pollution. In contrast, gelatin-based film lack of desired mechanical, thermal and water vapour barrier properties. Thus, this study aimed to investigate the mechanical and physical properties of bio-nanocomposite films based on chicken skin gelatin with different concentration of chitosan nanoparticles (CSNPs). Gelatin/CSNPs film solutions with different CSNPs concentration (0-8%, w/w) were stirred at 45oC for 30 min and oven-dried at 45oC. Film characterization determination includes tensile strength (TS), elongation at break (EAB), Young’s modulus (YM), water solubility, water vapour permeability (WVP), film morphology and melting temperature (Tm). Results of the study indicated that incorporation of CSNPs significantly influenced film properties. The addition of CSNPs increased the TS and YM value, which lead to stronger films than the pure chicken skin gelatin films. However the addition of CSNPs decreased the EAB value. Furthermore, WVP and water solubility significantly decreased (p < 0.05) by the addition of 6% CSNPs. Morphology images showed that increased CSNPs reduced the film’s amorphous character, especially in high level, in which higher CSNPs (8%) resulted in the aggregation of particles in the composites. The nano-reinforcement films showed higher thermal stability as compared to pure chicken skin gelatin films. In conclusion, the film with 6% CSNPs showed the best formulation, as it demonstrated high in TS, YM and Tm value, while low in EAB, water solubility and WVP value compared to other films. The results presented in this study showed the feasibility of using bio-nanocomposite technology to improve the properties of biopolymer films based on chicken skin gelatin.
Millions of Muslims from all over the world perform Umrah and Hajj every year. There were 250,000 pilgrims from Malaysia in 2017, and the number is expected to grow by 20 percent in 2018. This projected increase will create a huge demand for Umrah and Hajj travel agencies’ services in Malaysia. At present, there are 328 Umrah and Hajj travel agencies registered under Malaysia’s Ministry of Tourism and Culture (MOTAC). However, the supposedly bright outlook maybe marred by undesirable consequences. Along with the increase in the number of Umrah and Hajj travel agencies, there also tends to be an increase in fraud Umrah packages offered by fake agents in Malaysia. Such incidents will cause sadness and anger in the victims and other involved parties. Umrah package fraud involving fake agents in Malaysia has attracted the attention of many including the public, the government, the private sector, even the media. Nevertheless, studies on correlation between Muslim travellers’ perception of the quality of service by Umrah travel agencies and their decision to purchase the Umrah package are scarce in Malaysia. Hence, this study investigated Muslim travellers’ perception of Umrah and Hajj travel agencies’ service quality and its influence on their decision to purchase the Umrah package, in an attempt to discover why some Muslim travellers fell into fake agents’ trap in Malaysia. A total of 319 Muslim respondents completed the questionnaire on service quality perception, specifically service quality elements and loyalty. The data obtained was examined using SPSS version 25 for descriptive and regression analysis. Umrah and Hajj travel agencies as well as relevant government agencies could use the findings of the study to assist with formulating plans and policies to improve the quality of service by Umrah and Hajj industry players and awareness among Muslim travellers on the importance of being able to identify bogus agencies. The limitations, implications andsuggestions for future research are also discussed.
MeSH terms: Anger; Attention; Fraud; Government; Government Agencies; Islam; Malaysia; Perception; Surveys and Questionnaires; Regression Analysis; Travel; Private Sector; Policy
The main purpose of this research is to understand the experiences and psychological wellbeing of nurses working in palliative ward. There were two research questions driving this research throughout the process of data collection and data analysis. Four nurses from the palliative ward participated in this research to provide details on their psychological wellbeing. Semi-structured interview questions together with observation and simple survey questions were used to gather the data. Thematic analysis was used to identify the meaningful themes for each research question. Six main themes were identified: autonomy, environmental mastery, positive relation, personal growth, self-acceptance and purpose in life. The findings are highly relevant with the theory and literature. The findings indicate that the participants have good psychological wellbeing due to their huge experiences in palliative ward.
MeSH terms: Biological Phenomena; Humans; Surveys and Questionnaires; Comprehension
Bapa tunggal merupakan satu golongan yang kurang dititikberatkan oleh masyarakat termasuk agensi-agensi dan NGO yang memfokuskan kepada kebajikan masyarakat berbanding dengan ibu tunggal. Hal ini kerana kebanyakan masyarakat merasakan bahawa golongan ini memiliki kekuatan fizikal dan mental yang tinggi memandangkan bapa tunggal adalah kaum lelaki. Namun, tidak dinafikan bahawa golongan bapa tunggal ini juga mengalami tekanan serius kerana kehilangan satu semangat atau sokongan tanpa pasangan hidupnya. Mereka terpaksa menyandang pelbagai tugas dalam rumah tangga dan membesarkan anak-anak. Kajian terdahulu lebih memfokuskan kepada ibu tunggal yang mendapat sokongan sosial daripada pelbagai agensi. Maka tekanan dan sokongan sosial penting dalam permasalahan bapa tunggal terutama dalam pengurusan rumah tangga dan pekerjaan harian. Objektif utama kajian ini ialah meneroka tekanan dan sokongan sosial dalam kalangan bapa tunggal. Kajian ini dijalankan dengan menggunakan pendekatan kualitatif berpandukan kajian kes. Teknik pengumpulan data kajian adalah melalui temu bual berstruktur. Tujuh orang informan telah dipilih bagi kajian ini dengan menggunakan teknik persampelan bertujuan (purposive sampling). Lokasi kajian yang dipilih ialah daerah Kuala Selangor, Selangor. Data temu bual berstruktur dianalisis secara tematik dan hasil kajian mendapati terdapat tiga faktor utama yang mempengaruhi tekanan terhadap bapa tunggal iaitu emosi, pengurusan rumah tangga dan pekerjaan yang ditemukan menerusi transkrip informan kajian. Manakala sokongan sosial yang diperlukan oleh golongan bapa tunggal ialah kewangan, sokongan moral dan tempat bersembang. Implikasi kajian ini diharapkan dapat mencadangkan program advokasi yang sesuai kepada pekerja sosial dalam mengendalikan kes bapa tunggal dalam komuniti dan institusi.
Kajian ini bertujuan mengenal pasti hubungan antara kebimbangan dan kemurungan dengan kualiti hidup warga emas demensia di institusi jagaan warga emas. Kajian ini turut mencadangkan terapi kelompok reminiscence dalam menurunkan kebimbangan dan kemurungan dalam kalangan penghuni di institusi. Reka bentuk kajian yang digunakan ialah kajian keratan rentas dan telah dijalankan di Rumah Sri Kenangan Cheras. Seramai 58 warga emas demensia tahap ringan dan sederhana dipilih melalui proses penyaringan oleh pakar psikologi klinikal. Kajian kuantitatif ini telah menggunakan instrumen Quality of Life-AD (QoL-AD), Geriatric Depression Scale (GDS)) dan Beck Anxiety Inventory (BAI) untuk mengkaji hubungan antara kebimbangan dan kemurungan dengan kualiti hidup warga emas demensia. Hasil kajian menunjukkan terdapat hubungan yang tidak signifikan antara kualiti hidup dengan tahap kebimbangan. Manakala hubungan antara kualiti hidup dan tahap kemurungan warga emas demensia di RSK Cheras adalah signifikan. Pengaplikasian terapi kelompok reminiscence sebagai salah satu intervensi yang berkesan dalam membantu meningkatkan kualiti hidup dan menurunkan kebimbangan dan kemurungan dalam kalangan warga emas di institusi penjagaan telah dibincangkan.
MeSH terms: Anxiety; Depression; Mass Screening; Memory; Fenofibrate; Quality of Life; Ecological Momentary Assessment
Penggunaan media sosial tanpa budaya civik dan nilai etika dalam kalangan pelajar telah menjerumuskan ramai pelajar IPTA dalam kancah buli siber. Buli secara maya boleh dilakukan dalam muatnaik perkataan kejam, gambar dan video mangsa bagi menguggut, menghasut, fitnah, membalas dendam sehingga pemangsa merasakan dirinya hebat dan berkuasa. Durasi pantas semua maklumat boleh disebarluaskan dalam kadar pantas dengan jangkauan seluas dunia. Antara faktor buli siber berlaku dalam awal dewasa ialah cemburu, menanggih perhatian kawan media sosial, kapasiti ‘jaguh” atau gengster dalam media sosial. Kajian terdahulu lebih mefokuskan kepada fenomena buli dalam kalangan remaja, pelajar sekolah dan pengguna media massa. Manakala, amat kurang kajian berkaitan isu buli siber dalam kalangan mahawiswa. Objektif utama kajian ini ialah meneroka impak buli siber dalam kalangan pelajar UKM. Pendekatan kajian kes digunakan sebagai rekabentuk metodologi kajian ini. Pembinaan garis panduan soalan semi-struktur digunakan untuk temu bual responden. Seramai 6 orang responden telah dipilih bagi kajian ini dengan menggunakan teknik persampelan bertujuan (purposive sampling). Lokasi kajian yang dipilih ialah Universiti Kebangsaan Malaysia. Data temubual berstruktur dianalisis secara tematik. Hasil kajian mendapati terdapat lima kesan utama iaitu masalah emosi, kemurungan, keinginan untuk membunuh diri, kesedihan yang berlebihan, ketakutan dan perasaan marah tidak terkawal yang ditemukan transkrip responden kajian. Implikasi kajian ini diharapkan dapat mencadangkan program intervensi dan advokasi yang sesuai kepada pekerja sosial dalam mengendalikan kes buli siber di Bahagian Kaunseling Siswa, UKM.
MeSH terms: Autonomic Nervous System Diseases; Communications Media; Malaysia; Fenofibrate
Vibriosis is a prevalent aquatic disease caused by Vibrio species and has led to massive loss of brown-marbled grouper, Epinephelus fuscoguttatus. The complexity of molecular mechanisms associated with immune defence can be studied through transcriptomics analysis. High quality and quantity of total RNAs are crucial for the veracity of RNA sequencing and gene expression analysis. A low quality RNA will compromise downstream analysis, resulting in loss of time and revenue to re-acquire the data again. Thus, a reliable and an efficient RNA isolation method is the first and most important step to obtain high quality RNA for gene expression studies. There are many aspects need to be considered when deciding an extraction method, such as the cost-effectiveness of the protocol, the duration of chemical exposure, the duration required for a complete extraction and the number of sample-transferring. A good RNA extraction protocol must be able to produce high yield and purity of RNA free from enzyme inhibitors, such as nucleases (RNase), phenols, alcohols or other chemicals carryover, apart from protein and genomic DNA contamination, to maintain isolated RNA integrity in storage condition. In this study, TransZolTM Up produced clean and pure RNA samples from control gills only but not from the infected gill and whole-body tissues. Modified conventional CTAB (conventional hexadecyltrimethylammonium bromide) method was then used as an alternative method to isolate RNA from gill and whole-body tissues of Vibrio-infected E. fuscoguttatus. Modified CTAB method produced intact RNA on gel electrophoresis with higher RIN number (>6.5) for infected gill and whole-body tissues, suggesting that this method could also be used to isolate high quality RNA from fish samples. Therefore, this method is potentially suitable to be used to extract RNA from other fish species especially those that have been infected.