Browse publications by year: 1994

  1. Boo NY, Lye MS, Ong LC
    Singapore Med J, 1994 Apr;35(2):163-6.
    PMID: 7939812
    A cross-sectional study was carried out on 8,478 consecutive normal singleton Malaysian neonates born in the Maternity Hospital, Kuala Lumpur. The objectives were to compare the mean birth-weights, crown-heel lengths and head circumferences of Malay, Chinese and Indian infants at gestation age from 28 to 42 weeks, and to construct the Malaysian growth charts. Above the gestation age of 34 weeks, the birthweights were significantly influenced by maternal gravida status (p < 0.03), ethnic origin (p < 0.001) and/or sex of the neonates (p < 0.026). Above this gestation age, neonates of multigravida mothers were significantly heavier than those of primigravida mothers; Indians were significantly lighter than Malays and Chinese; and males were significantly heavier than females. The head circumferences and body lengths of neonates were significantly influenced by ethnic origin, sex and/or maternal gravida status at gestation above 35 and 36 weeks respectively (p < 0.05). It was most likely due to the small sample size which explained our inability to detect statistically significant difference in all measurements (birthweight, length and head circumference) by sex, ethnicity and maternal gravida status at gestation below 35 weeks. Based on the measurements obtained in this study, percentile charts for the Malaysian population were constructed and made available for the first time. These charts will be useful for the assessment of Malaysian neonates during the perinatal period.
    MeSH terms: Birth Weight; Body Height; Cephalometry/statistics & numerical data; Cross-Sectional Studies; Cross-Cultural Comparison*; Ethnic Groups/statistics & numerical data*; Female; Fetal Growth Retardation/ethnology*; Humans; Infant, Newborn; Malaysia/epidemiology; Male; Pregnancy; Pregnancy Trimester, Third; Reference Values
  2. Ismail Y, Loo CS, Zahary MK
    Singapore Med J, 1994 Apr;35(2):171-2.
    PMID: 7939814
    We reviewed 116 chest radiographs done in 70 adult asthmatic patients who were admitted to the Hospital Universiti Sains Malaysia from January to December 1989. The chest radiographs were abnormal in 23% of cases. Twelve percent showed hyperinflation and 7% had pneumonia. Eight patients diagnosed clinically to have pneumonia had normal chest radiographs. Seven patients had radiographic findings of conditions which were unsuspected clinically. These included two cases of pneumonia, one case each of fibrosing alveolitis, pneumothorax, pneumomediastinum, mitral stenosis with left ventricular failure and right pleural effusion. In conclusion, we found that significant chest radiograph abnormalities in adult patients admitted for asthma were uncommon although chest radiographs were helpful in detecting complications or coincidental conditions. Chest radiograph is therefore an important investigation in adult asthmatic patients who are admitted. However, considering the cost and the risk of radiation, it should be done only in selective cases rather than as a routine procedure.
    Study site: Hospital Universiti Sains Malaysia, Kelantan, Malaysia
    MeSH terms: Adolescent; Adult; Asthma/radiography*; Child; Female; Humans; Lung Diseases, Obstructive/radiography*; Malaysia; Male; Middle Aged; Patient Admission*; Retrospective Studies
  3. Ram SP, Noor AR, Ariffin WA, Ariffin NA
    Singapore Med J, 1994 Apr;35(2):205-7.
    PMID: 7939823
    A 37-week gestation male boy was born to a gravida seven para six mother by spontaneous vertex delivery at home. The baby cried at birth. On day 3 of life, he was admitted for respiratory distress. Physical examination revealed ectrodactyly, thin dry skin, anomalous tear duct with cardiomegaly. X-ray revealed absent radii, cardiomegaly and hemivertebra at L1. Echocardiogram revealed perimembranous type of ventricular septal defect. A diagnosis of Ectodermal Dysplasia Ectrodactyly Clefting Syndrome with ventricular septal defect was made. He was managed conservatively in the nursery. However, he expired on day 27 of life following short spell of fever apnoeic episode due to neonatal sepsis.
    MeSH terms: Abnormalities, Multiple/genetics*; Cleft Lip/genetics*; Cleft Palate/genetics*; Ectodermal Dysplasia/genetics*; Ectromelia/genetics*; Heart Septal Defects, Ventricular/genetics*; Humans; Infant, Newborn; Male; Pedigree; Radius/abnormalities; Syndrome
  4. Malik AS, Zabidi MH, Noor AR
    Singapore Med J, 1994 Apr;35(2):215-6.
    PMID: 7939826
    Traditional medicine is practised to some degree in all cultures. Many different types of herbal preparations and "oils" are widely used in Malaysia, too. We report a case of acute salicylism due to accidental ingestion of one brand of such oils. Compulsory labelling of traditional drugs with their chemical ingredients is suggested for proper and timely management of such cases.
    MeSH terms: Blood Chemical Analysis; Epilepsy, Tonic-Clonic/blood; Epilepsy, Tonic-Clonic/chemically induced; Humans; Infant; Malaysia; Male; Medicine, Traditional*; Metabolic Clearance Rate; Salicylates/pharmacokinetics; Salicylates/poisoning*; Drug Overdose/blood; Drug Overdose/etiology*
  5. Hassan H, Teh A
    Singapore Med J, 1994 Apr;35(2):217-8.
    PMID: 7939827
    Clostridium septicum infection has been shown to have a strikingly high association with either bowel or blood malignancies. The infection may be fatal if unrecognised. We report a case of C. septicum bacteremia in a man diagnosed with acute myeloid leukaemia.
    MeSH terms: Adult; Clostridium/isolation & purification*; Clostridium/pathogenicity; Clostridium Infections/microbiology*; Enterocolitis, Pseudomembranous/microbiology; Humans; Male; Opportunistic Infections/microbiology*; Virulence; Leukemia, Myeloid, Acute/complications*; Leukemia, Myeloid, Acute/microbiology; Sepsis/microbiology*
  6. Ram SP, Kyaw K, Noor AR
    Trop Doct, 1994 Apr;24(2):81-2.
    PMID: 8009626
    MeSH terms: Diagnosis, Differential; Hematoma/diagnosis; Hematoma/etiology*; Humans; Infant, Newborn; Malaysia; Male; Massage/adverse effects*; Medicine, Traditional; Testicular Diseases/diagnosis; Testicular Diseases/etiology*
  7. Raj SM, Anteson R, Sivakumaran S, Abdullah MR, Low HC
    Trop Doct, 1994 Apr;24(2):88-9.
    PMID: 8009635
    MeSH terms: Adolescent; Adult; Aged; Aged, 80 and over; Ascariasis/complications*; Ascariasis/epidemiology; Female; Hookworm Infections/complications*; Hookworm Infections/epidemiology; Humans; Malaysia/epidemiology; Male; Middle Aged; Parasite Egg Count; Trichuriasis/complications*; Trichuriasis/epidemiology; Abdominal Pain/etiology*; Abdominal Pain/epidemiology
  8. Yap KL, Lam SK
    J Virol Methods, 1994 Apr;47(1-2):217-26.
    PMID: 8051228
    A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) was developed for the fast-growing and cytopathic cell culture-adapted hepatitis A virus (HAV) strain HM175A.2. Infectivity titration by EIA correlated well with titration by cytopathic effects. The reliability of this assay was demonstrated by close agreement in virus infectivity titers among different assays of the same virus aliquot and between assays of different virus aliquots. HAV infected cell cultures after fixation could be stored for up to 1 week before testing without decline in virus titer.
    MeSH terms: Antigens, Viral/analysis; Cells, Cultured; Cytopathogenic Effect, Viral; Hepatitis A/microbiology*; Hepatitis A/pathology; Hepatovirus/immunology; Hepatovirus/isolation & purification*; Humans; Immunoenzyme Techniques*; Tissue Preservation; Reproducibility of Results; Observer Variation; Hepatitis A Antigens
  9. Ahmad M, Roy RA
    Endod Dent Traumatol, 1994 Apr;10(2):71-6.
    PMID: 8062810
    The incidence of breakage of Piezon-Master ultrasonic K files were evaluated. Three groups of unused files were subjected to three treatments, namely; free vibration in air without irrigation, free vibration in root canal while minimizing contact with the wall of canal in the presence of irrigation and light filing in root canal with free flow of irrigation. Cavitation produced by files in contact and free of contact with a glass surface was examined in order to observe the relationship between cavitation defects and breakage. In addition, the fractured and unfractured files were examined under a scanning electron microscope for the presence of cavitation pits. The results indicated that more files broke in air. In water, a higher incidence of breakage occurred when files were allowed to freely vibrate while no breakage occurred when the files were used in filing. All files generated cavitation which resulted in pitting of their surfaces. However, it was considered unlikely that the pits contributed to fracture. Fatigue cracks which could be the result of the manufacturing process were observed at some of the corners of the cross sections of the fractured files and could be the main contributory factor to fracture.
    MeSH terms: Chemistry, Physical; Equipment Failure; Materials Testing; Metals/chemistry; Microscopy, Electron, Scanning; Root Canal Therapy/instrumentation*; Surface Properties; Ultrasonic Therapy/instrumentation; Vibration; Chemical Phenomena
  10. Cheah PL, Looi LM
    Pathology, 1994 Apr;26(2):115-8.
    PMID: 8090580
    Examination of routinely stained haematoxylin and eosin sections may sometimes prove inadequate in differentiating partial hydatidiform moles (PHM) from complete hydatidiform moles (CHM). While cytogenetic analysis can aid in the distinction, such facilities are not always available. The possibility of using immunohistochemistry to aid in the differentiation was studied. Twenty-five histologically proven CHM and 11 PHM were studied for their patterns of expression of human chorionic gonadotrophin (hCG), human placental lactogen (hPL) and placental alkaline phosphatase (PIAP). All CHM stained diffusely with hCG and focally with both hPL and PIAP irrespective of gestational age. Of PHM, 63.6% were diffusely positive for hCG, 27.3% for hPL and 54.5% for PIAP; the rest were focally positive. The hCG pattern changed from diffuse to focal with increasing gestational age of PHM, while those of hPL and PIAP became increasingly diffuse with gestational age. While these protein expressions may be applied in differentiating late PHM from CHM, it is not useful in first trimester cases. The most helpful application is that focal expression of hCG and diffuse expressions of hPL and PIAP is not seen in CHM, thereby excluding such a diagnosis. PHM, in contrast, can show either diffuse or focal expression of all 3 antigens.
    MeSH terms: Adult; Alkaline Phosphatase/analysis*; Female; Chorionic Gonadotropin/analysis*; Humans; Hydatidiform Mole/diagnosis; Hydatidiform Mole/pathology; Hydatidiform Mole/chemistry*; Immunohistochemistry; Isoenzymes/analysis*; Placental Lactogen/analysis*; Pregnancy; Retrospective Studies; Uterine Neoplasms/diagnosis; Uterine Neoplasms/pathology; Uterine Neoplasms/chemistry*
  11. Sundram K, Hayes KC, Siru OH
    Am J Clin Nutr, 1994 Apr;59(4):841-6.
    PMID: 8147328
    In a double-blind crossover study, 17 normocholesterolemic male volunteers were fed carefully designed whole-food diets in which 5% of energy was exchanged between palmitic (16:0) and lauric + myristic acids (12:0 + 14:0) whereas all other fatty acids were held constant. Resident males received each diet during separate 4-wk periods. The test diets supplied approximately 30% of energy as fat and 200 mg cholesterol/d. Compared with the 12:0 + 14:0-rich diet, the 16:0-rich diet produced a 9% lower serum cholesterol concentration, reflected primarily by a lower (11%) low-density-lipoprotein-cholesterol concentration and, to a lesser extent, high-density-lipoprotein cholesterol. No diet-induced changes were noted in the cholesterol content of other lipoproteins, nor did exchange of saturated fatty acids affect the triglyceride concentration in serum or lipoprotein fractions. These data indicate that a dietary 12:0 + 14:0 combination produces a higher serum cholesterol concentration than does 16:0 in healthy normocholesterolemic young men fed a low-cholesterol diet.
    MeSH terms: Adult; Apolipoproteins/analysis; Cholesterol/blood*; Dietary Fats/pharmacology*; Double-Blind Method; Fatty Acids/pharmacology*; Humans; Lauric Acids/pharmacology; Male; Myristic Acids/pharmacology; Palmitic Acids/pharmacology; Triglycerides/blood
  12. Wong KT, Pathmanathan R
    J Parasitol, 1994 Apr;80(2):327-30.
    PMID: 8158479
    The ultrastructure of the human skeletal muscle sarcocyst found in Malaysia is reported. Sarcocyst-positive, formalin-fixed tongue tissues were postfixed in osmium tetroxide. The primary cyst wall consisted of a thin membrane supported by osmiophilic material that was interrupted regularly by vesicle-like invaginations. Although there were no cytophaneres, stubby protrusions of the primary wall were observed. These protrusions were accentuated by dense, curvilinear material externally. The primary wall was wavy over about half the cross section of the cyst. The granular ground substance underlying the primary wall occasionally contained hitherto undescribed coiled microtubular structures. Branching septa extended from the ground substance into the cyst, separating mature merozoites into compartments. A few peripheral metrocytes and many laminated myelin figure-like structures, probably degenerating merozoites, were found. Although the human muscular sarcocyst has the same basic ultrastructure as those found in other animals, the stubby protrusions and coiled microtubular structures in the ground substance have not been described previously in nonhuman animals.
    MeSH terms: Animals; Humans; Microscopy, Electron; Muscles/parasitology*; Sarcocystis/ultrastructure*; Sarcocystosis/parasitology*
  13. Manonmani V, Wallace SJ
    Arch Dis Child, 1994 Apr;70(4):288-90.
    PMID: 8185360
    The cases are described of eight children, five of them girls, who had epilepsy with myoclonic absences. The mean age of onset was 4.9 years. Brief episodes of loss of awareness with bilateral clonic jerking of the upper limbs were associated with rhythmic 3 cycles/second spike-wave discharges on electroencephalogram. Generalised tonic-clonic or astatic seizures, or both, also occurred in seven patients. All now have learning difficulties, and seven have behavioural problems. Conventional treatment for absences was effective in only two children. Of six patients treated with lamotrigine, five have improved substantially, but only one is in sustained complete remission. One recently diagnosed patient continues to have frequent myoclonic absences. As the response to treatment and long term outcome are much poorer, it is important to differentiate myoclonic absences from typical childhood absence epilepsy.
    MeSH terms: Anticonvulsants/therapeutic use; Brain/physiopathology; Child; Child Behavior Disorders/etiology; Child, Preschool; Electroencephalography; Epilepsy, Absence/diagnosis*; Epilepsy, Absence/drug therapy; Epilepsy, Absence/psychology; Female; Follow-Up Studies; Humans; Infant; Learning Disorders/etiology; Male; Prognosis; Triazines/therapeutic use
  14. Ng TP, Hui KP, Tan WC
    Thorax, 1994 Apr;49(4):347-51.
    PMID: 8202905
    BACKGROUND: The prevalence and morbidity of asthma vary greatly among different ethnic communities and geographical locations, but the roles of environmental and genetic factors are not fully understood. The differences in prevalence of adult asthma among Chinese, Malay, and Indian ethnic groups in Singapore were examined, and the extent to which these could be explained by personal and environmental factors were investigated.
    METHODS: A stratified disproportionate random sample (n = 2868) of Chinese (n = 1018), Malays (n = 967), and Indians (n = 883) of both sexes was drawn from households in five public housing estates, and an interviewer administered questionnaire was used to determine cumulative and current prevalence of "physician diagnosed asthma" (symptoms with a physician diagnosis of asthma).
    RESULTS: Lifetime cumulative prevalence (standardised to the general population) of "physician diagnosed asthma" was 4.7% in men and 4.3% in women; 12 month period prevalences were 2.4% and 2.0%, respectively. Cumulative prevalence of asthma was significantly higher in Indians (6.6%) and Malays (6.0%) than in Chinese (3.0%); period prevalences of asthma were 4.5% in Indians, 3.3% in Malays, and 0.9% in Chinese. Ownership of cats or dogs was more frequent in Malays (15.4%) and Indians (11.2%) than in Chinese (8.8%). Rugs and carpets were also more frequently used by Malays (52.2%) and Indians (40.7%) than by Chinese (8.9%). Current smoking prevalences were higher in Malays (27.3%) than in Indians (19.4%) and Chinese (23.0%). Malays and Indians did not have higher rates of atopy (11.1% and 15.2%, respectively) than Chinese (15.4%). Adjustment for these factors in multivariate analyses reduced the greater odds of asthma in Malays and Indians, but not to a significant extent.
    CONCLUSIONS: There are ethnic differences in the prevalence of asthma in Singapore which are not entirely explained by differences in smoking, atopy, or other risk factors. Other unmeasured environmental factors or genetic influences are likely to account for residual differences in the prevalence of asthma.
    MeSH terms: Adult; Aged; Asthma/etiology; Asthma/epidemiology*; China/ethnology; Female; Humans; India/ethnology; Interior Design and Furnishings; Malaysia/ethnology; Male; Middle Aged; Random Allocation; Risk Factors; Singapore; Smoking/epidemiology; Urban Population; Prevalence; Multivariate Analysis
  15. Zinn JS, Kashlak RJ, Balotsky ER
    Hosp Health Serv Adm, 1994;39(1):17-30.
    PMID: 10132097
    As growth potential in the U.S. market declines and regulatory constraints increase, providers of health-related services may look increasingly to international opportunities as a way to supplement the lost domestic market. In this article, critical factors bearing on the decision to compete in international markets are identified. Existing theories of multinational competition are expanded to provide a framework for analyzing international competition. Applied in the context of the proprietary hospital industry, the critical factors governing both the selection of foreign markets and mode of entry are proposed to be host country receptivity and market growth potential.
    MeSH terms: Australia; Brazil; Contract Services/economics; Economic Competition/trends*; Great Britain; Hospital Planning/trends; Hospitals, Proprietary/economics*; Hospitals, Proprietary/trends; International Cooperation*; Investments/economics; Malaysia; Middle East; Multi-Institutional Systems/economics*; Multi-Institutional Systems/trends; Organizational Objectives; Ownership/economics; Singapore; United States; Models, Organizational
  16. Liong ML, Suzuki T, Yamanaka H, Kurokawa K, Daikuzono N, Nakazato M
    J Clin Laser Med Surg, 1994 Apr;12(2):85-92.
    PMID: 10151050
    Prostalase¿ has a probe that emits a laser beam at 360 degrees . Targeted obstructive prostatic adenoma tissue was heated to above the cytotoxic threshold temperature of 45 degrees C. After successful canine prostate study, from September 1992 to April 1993, 45 patients were treated. This paper reports the 9 months results of this initial cohort of patients. Prostatic and periprostatic temperature mapping showed the mean temperature within the adenoma zone was 49 degrees C, while the periprostatic tissue remained within the safety level of less than 42.5 degrees C. The mean prostate volume reductions at 3, 6, and 9 months were 36, 33, and 38%, respectively. Those patients whose surgery was unsuccessful had prostatic tissue removed by TURP at 2 to 3 months. This tissue revealed a definite zone of coagulative necrosis. For the clinical assessment, patients were divided into urine retention (UR) and nonretention (NR) subgroups. At 9 months, 20 of the 32 UR subgroup and 10 of the 13 NR subgroup patients were available for assessment. Due to poor response or complications, 6 of the 26 UR patients (23%) required ancillary treatment. Hence, 20 of the 26 cases (77%) remained catheter free and their mean maximum uroflow +/- SE was 9.6 +/- 0.7 ml/sec. Based on a Siroky normogram only 7 of these 26 patients (27%) became unobstructed. Two of the 12 NR subgroup patients (17%) required ancillary treatment. The mean maximum uroflow +/- SE was 10.7 +/- 1.2 ml/sec.(ABSTRACT TRUNCATED AT 250 WORDS)
    MeSH terms: Equipment Design; Humans; Hyperthermia, Induced/adverse effects; Hyperthermia, Induced/instrumentation*; Hyperthermia, Induced/methods; Prostate/anatomy & histology; Prostatic Hyperplasia/therapy*; Urodynamics; Treatment Outcome; Laser Therapy*
  17. Grove-White RJ
    Proc R Coll Physicians Edinb, 1994 Apr;24(2):267-75.
    PMID: 11639254
    MeSH terms: Humans; Malaysia; Medicine*; Singapore; Warfare*; History, 20th Century
  18. Spaan E
    Int Migr Rev, 1994;28(1):93-113.
    PMID: 12287280
    "This article discusses international migration from Java in the past and present and the role brokers have played in stimulating this movement. It describes legal and clandestine labor migration to Singapore, Malaysia, and Saudi Arabia, the influence of employment brokers on the process, and the organization of the recruitment networks. The involvement of brokers is crucial but not always beneficial for the migrants. Migrants are dependent on the brokers and risk exploitation. In the case of movement to Saudi Arabia, there is a linkage with religious institutions and the Islamic pilgrimage."
    MeSH terms: Asia; Asia, Southeastern; Asia, Western; Communication; Demography; Developing Countries; Economics; Emigration and Immigration*; Employment*; Health Manpower; Health Planning; Indonesia; Health Knowledge, Attitudes, Practice; Leadership*; Malaysia; Middle East; Organization and Administration; Personnel Selection*; Population; Population Dynamics; Saudi Arabia; Singapore; Transients and Migrants*
  19. Mathew A, Cheng HM, Sam CK, Prasad U
    Clin. Immunol. Immunopathol., 1994 May;71(2):164-8.
    PMID: 7514112
    Inhibition studies were carried out to study possible cross-reactivity between a peptide fragment of the Epstein-Barr virus nuclear antigen, EBNA-1, and keratin/collagen. The 20-amino acid peptide (pAG), derived from a glycine-alanine repeat region of EBNA-1, uniquely makes up about one-third of the viral protein and is a dominant IgA antigenic epitope in patients with nasopharyngeal carcinoma (NPC). A small percentage of normal human sera (NHS) also binds pAG and this reactivity is examined in this study. Ten percent (2/20) and 13.4% (2/15) of IgA-pAG-positive NPC sera and NHS, respectively, were significantly inhibited by keratin in a competitive ELISA system. Conversely, 31.6% (6/19) and 30.8% (4/13) of IgA-keratin-positive NPC sera and NHS, respectively, were significantly inhibited by pAG. This indicated minimum cross-reactivity between IgA serum antibodies to EBNA-1 and keratin. Using collagen as inhibitor, none of 18 and only 2/13 IgA-pAG-positive NPC sera and NHS, respectively, were inhibited. In the collagen ELISA system, only 2/19 (10.5%) and 4/25 (16%) of IgA-collagen-positive NPC sera and NHS, respectively, were inhibited with pAG. Therefore, cross-reactivity with collagen was also low. IgA-pAG-positive NHS may therefore not be a false positive phenomenon, but whether it may represent an early serological profile related to NPC carcinogenesis remains to be determined.
    MeSH terms: Alanine/immunology*; Amino Acid Sequence; Antigens, Viral/immunology*; Binding, Competitive; Collagen/immunology*; Cross Reactions; DNA-Binding Proteins/immunology*; Enzyme-Linked Immunosorbent Assay; Herpesvirus 4, Human/immunology; Glycine/immunology*; Humans; Immunoglobulin A/blood; Immunoglobulin A/immunology*; Keratins/immunology*; Molecular Sequence Data; Nasopharyngeal Neoplasms/blood; Nasopharyngeal Neoplasms/immunology*; Repetitive Sequences, Nucleic Acid; Epstein-Barr Virus Nuclear Antigens
  20. Thong KL, Cheong YM, Puthucheary S, Koh CL, Pang T
    J Clin Microbiol, 1994 May;32(5):1135-41.
    PMID: 7914202
    Pulsed-field gel electrophoresis (PFGE) was used to compare and analyze 158 isolates of Salmonella typhi from five well-defined outbreaks of typhoid fever in Malaysia and also isolates involved in sporadic cases of typhoid fever occurring during the same period. Digestion of chromosomal DNAs from these S. typhi isolates with the restriction endonucleases XbaI (5'-TCTAGA-3'), SpeI (5'-ACTAGT-3'), and AvrII (5'-CCTAGG-3') and then PFGE produced restriction endonuclease analysis (REA) patterns consisting of 11 to 24 DNA fragments ranging in size from 20 to 630 kbp. Analysis of the REA patterns generated by PFGE after digestion with XbaI and SpeI indicated that the S. typhi isolates obtained from sporadic cases of infection were much more heterogeneous (at least 13 different REA patterns were detected; Dice coefficient, between 0.73 and 1.0) than those obtained during outbreaks of typhoid fever. The clonal nature and the close genetic identities of isolates from outbreaks in Alor Setar, Penang, Kota Kinabalu, Johor Bahru, and Kota Bahru were suggested by the fact that only a limited number of REA patterns, which mostly differed by only a single band, were detected (one to four patterns; Dice coefficient, between 0.82 and 1.0), although a different pattern was associated with each of these outbreaks. Comparison of REA patterns with ribotyping for 18 S. typhi isolates involved in sporadic cases of infection showed a good correlation, in that 72% of the isolates were in the same group. There was no clear correlation of phage types with a specific REA pattern. We conclude that PFGE of s. typhi chromosomal DNA digested with infrequently cutting restriction endonucleases is a useful method for comparing and differentiating S. typhi isolates for epidemiological purposes.
    MeSH terms: Base Sequence; Disease Outbreaks*; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Epidemiologic Methods; Humans; Malaysia/epidemiology; Polymorphism, Restriction Fragment Length; Salmonella typhi/classification; Salmonella typhi/genetics; Salmonella typhi/isolation & purification*; Typhoid Fever/microbiology*; Typhoid Fever/epidemiology*; Deoxyribonucleases, Type II Site-Specific; Electrophoresis, Gel, Pulsed-Field
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