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A device to facilitate preparation of high-density neural cell cultures in MEAs

Mok SY, Lim YM, Goh SY

J Neurosci Methods, 2009 May 15;179(2):284-91.
PMID: 19428539 DOI: 10.1016/j.jneumeth.2009.02.009

A device to facilitate high-density seeding of dissociated neural cells on planar multi-electrode arrays (MEAs) is presented in this paper. The device comprises a metal cover with two concentric cylinders-the outer cylinder fits tightly on to the external diameter of a MEA to hold it in place and an inner cylinder holds a central glass tube for introducing a cell suspension over the electrode area of the MEA. An O-ring is placed at the bottom of the inner cylinder and the glass tube to provide a fluid-tight seal between the glass tube and the MEA electrode surface. The volume of cell suspension in the glass tube is varied according to the desired plating density. After plating, the device can be lifted from the MEA without leaving any residue on the contact surface. The device has enabled us to increase and control the plating density of neural cell suspension with low viability, and to prepare successful primary cultures from cryopreserved neurons and glia. The cultures of cryopreserved dissociated cortical neurons that we have grown in this manner remained spontaneously active over months, exhibited stable development and similar network characteristics as reported by other researchers.

Matched MeSH terms: Neurophysiology/instrumentation*; Neurophysiology/methods
Fulltext Developing Normative Reference Values for Nerve Conduction Studies of Commonly Tested Nerves among a Sample Pakistani Population

Shivji Z, Jabeen A, Awan S, Khan S

J Neurosci Rural Pract, 2019 4 20;10(2):178-184.
PMID: 31001002 DOI: 10.4103/jnrp.jnrp_370_18

Introduction: Most neurophysiology departments around the world establish their own normative data. However, ethnic differences are not taken into account. Our aim was to establish normal nerve conduction studies (NCS) data for routinely tested nerves in individuals of Pakistani (South Asian) origin and to compare with Western published data.
Materials and Methods: One hundred healthy adults' nerves were assessed, using standardized techniques. Individuals were grouped into age groups. Gender differences were assessed.
Results: Of the 100 volunteers, 49 were female and 51 were male. Their mean age was 39.8 years. Findings showed statistically significant prolongation of median distal motor latency (DML) and F-wave latency with age and reduction of median, ulnar, and sural sensory amplitudes as age increased. Gender differences showed consistent difference in the normal values for median, ulnar, and peroneal DMLs and respective F-wave latencies, which were significantly shorter in females. Sensory amplitudes of tested upper extremity nerves were significantly lower in males. Comparing with available data, our findings are similar to the Saudi population but significantly different from the American and multiethnic Malaysian populations. Pakistani individuals generally have significantly higher amplitudes and faster conduction velocities with similarities to South Asian studies.
Conclusions: We recommend normative NCS parameters for commonly tested nerves for the Pakistani population, using standardized techniques to ensure highest quality testing and outcomes.

Matched MeSH terms: Neurophysiology
Fulltext The beck anxiety inventory for Malays (BAI-Malay): a preliminary study on psychometric properties

Firdaus, M., Nor Sheereen, Z.

Malaysian Journal of Medicine and Health Sciences, 2011;7(1):75-81.
MyJurnal

Introduction: The purpose of the present study was to investigate exploratory factor analysis of the
Beck Anxiety Inventory (BAI) and reports its reliability and validity in Malaysia. Method: One thousand
and ninety participants from four different samples (namely students, general population, medical and
psychiatric patients) completed the Malay version of Beck Anxiety Inventory, as well as additional
questionnaires such as Fear Questionnaire, Anxiety Sensitivity Index, Depression Anxiety Stress Scale, and Catastrophic Cognition Questionnaire. Results: Exploratory factor analysis revealed a three factor solution and accounted for 48.01% of the total variance. The three-factor structure appeared to be; subjective anxiety, autonomic, and neurophysiology. The Cronbach alpha coefficients (α) ranged from 0.66 to 0.89 with satisfactory overall alpha value (.91). Evidence was also found acceptable concurrent validity of the BAI-Malay (range between r=.22 to r=.67). Conclusion: This study shows that the BAIMalay is a reliable and valid instrument to measure symptoms of anxiety in the Malay population
and can be used in research and clinical service in Malaysia. However, replication of study by using
confirmatory factor analysis and application of the instrument among anxiety patients worth of further
investigation.

Matched MeSH terms: Neurophysiology
Population genetic variation of SLC6A4 gene, associated with neurophysiological development

Hande SH, Krishna SM, Sahote KK, Dev N, Erl TP, Ramakrishna K, et al.

J Genet, 2021;100.
PMID: 33764333

The serotonin transporter 5-HTT is encoded by a single gene SLC6A4. Polymorphisms in SLC6A4 has been associated with a wide variety of neurological and psychiatric disorders including increased risk of posttraumatic stress disorder, higher likelihood for depression, obsessive-compulsive disorder (OCD), increased hostility and criminal behaviour. Genes associated with complex diseases often exhibit strong signatures of purifying selection compared to others. Further, discernible population specific variation in the signature of natural selection have been observed for several complex disease-related genes. In this project we aimed to investigate the population genetic variation of the serotonin transporter gene (SLC6A4), focussing on the single nucleotide polymorphisms (SNPs). To this end, we employed 2504 individuals around the globe available in 1000 Genome project Phase III data and classified them into five ethnic groups: Americans (AMR), Europeans (EUR), Africans (AFR), East Asians (EAS) and South Asians (SAS). Principal component analysis (PCA) performed on all annotated SNPs of SLC6A4 depicted clear clustering between Africans and the rest of the world along PC1, and East Asians and other non-African populations along PC2. Further, these SNPs were found to be under strong selection pressure especially among East Asian populations with significantly high positive cross-population extended haplotype homozygosity scores compared to Africans, indicating that SLC6A4 has likely undergone a strong selective sweep among the East Asians in the recent past. Our study can potentially explain the association between polymorphisms in SLC6A4, and major depression and suicidal tendencies among people of East Asian ancestry and the absence of such associations among people of European ancestry.

Matched MeSH terms: Neurophysiology