Severe early childhood caries (SECC) is a microbialinfection that severely compromises the dentition ofyoung children.The aim of this study is toidentify bacteria associated with SECC and compare bacterial species between SECC and caries-free children. Bacterial samples were isolated from dental plaque samples of 3 to 6-yearold preschool children and the status of dental caries at the surface level was evaluated using World Health Organization (WHO) diagnostic criteria for decayed, missing and filled tooth surfaces (DFMS). 30 samples from the interproximal part were collected and genomic DNA was extracted using MasterPure™ Gram Positive DNA Purification Kit. Polymerase Reaction (PCR) was conducted on the genomic DNA extracted to amplify 16S rRNAgene and successfully amplified 16S rRNAgene from the samples was sent for sequencing.This research was approved by the IIUM Research Ethic Committee (IREC). Using the selected kit, genomic DNA was successfully extracted and PCR amplification of the bacterial 16S rRNAgeneusing universal bacterial primers was achieved giving a product of 1500 bp. Results from sequencing when analysedusing BLAST from the NCBI website, identified bacteria from the genus Vibrio, Haemophilusand Aggregatibacterfrom SECC samples while for caries-free samples Granulicatellawas identified.
Dens evaginatus (DE) is a developmental anomaly. It is an extra cusp or tubercle that protrudes from the occlusal surface of posterior teeth, as well as the lingual surface of anterior teeth. Tubercles are susceptible to pulp exposure from wear or fracture because of malocclusion;leading to pulpal complications early after eruption.DE may also complicate the process of daily routine oral health care. A 13-year-old girl presented to our polyclinic with sinus track at the posterior lower left buccal gingiva forthe past1 year. All 35 teeth were sound. At the lingual side, there was an accessory tooth in close relation to tooth 35 covered with supra-gingival inflammation. Electric pulp test (EPT) showed that tooth 35 was already non-vital. Intra-oral periapical (IOPA) radiograph and cone beam computed tomography (CBCT) scan showed an abnormal-appearing root which shows DE with radiolucency at the periapical area. Root canal treatment was performed using crown down pressureless (CDP) technique and obturation was done using lateral condensation technique. The tooth was restored using composite restoration. DE is a rare condition. It is quite a challenge for dental practitioner since the diagnosis is difficult and the treatment options are limited. In the case of DE with necrotic pulp and periapical abscess, root canal treatment is a treatment of choice. We have to accept and appreciate any gift from Allah SWT whether it is good or bad, no equal divine creation except from Allah SWT.Therefore we need to take care of our oral hygiene to prevent diseases. In every disease, there is a cure; we thus need to try to do the best to find the cure and to not easily get rid of the tooth and replace it with a denture. Early diagnosis of DE can lead to proper treatment which can result in high success rate if it is followed by adequate restoration.
: Natal tooth and neonatal tooth is a rare occurrence but can have a significant impact
on lactation. A 45-day-old baby girl was referred for problem with breastfeeding. Her mother
complained of painful nipple during nursing for the past two weeks. (Copied from article).
The aim is to investigate the dental development (DD) in children with hypodontia and hyperdontia compared to age, gender, and race matched controls.A match-pair case control study was conducted among children aged 5 to 14-year-old, attending the Dental Polyclinic, IIUM, from December 2011 until September 2014. Willem’s method was used to analyze the dental age (DA) using panoramic radiograph. The difference between dental age of hypodontia/hyperdontia children and the controlswas analyzed using analysis of covariance (ANCOVA). The differences between dental and chronological ages (CA) and the differences in DA and CA with the numbers of missing teeth were analyzed using t-test. Intra-class correlation coefficient between examiners ranged from 0.98-0.99. The result is that27 hypodontia and 12 hyperdontia samples were recruited and compared to a total of 78 matched controls. Both male (n=12) and female (n=15) hypodontia children had statistically significant delay in DD (p=0.000). There was no significant difference in the DD of male (n=7, p=0.811) and female (n=5, p=0.235) in hyperdontia children compared to the matched controls. No differences were observed between DA and CA with the number of missing teeth. Unlike hyperdontia children, hypodontia children showed delayed DD in comparison to age, gender, and race matched controls. There was no association between magnitudes of dental development delay in hypodontia children with the number of missing teeth. This study provides valuable baseline information for provision of better treatment planning for those hypodontia and hyperdontia children that may involve inter-disciplinary management.
Non-syndromic tooth agenesis defined as developmental absence of more than one
tooth that appears as independent congenital oral trait. Its prevalence, pattern and distribution
rates vary by populations. The aim of this study was to identify the pattern and distribution of
tooth agenesis in permanent dentition among IIUM dental polyclinic patients. (Copied from article).