For promoting in situ conservation, it is important to estimate the density distribution of fertile individuals, and there is a need for developing an easy monitoring method to discriminate between physiological states. To date, physiological state has generally been determined by measuring hormone concentration using radioimmunoassay or enzyme immunoassay (EIA) methods. However, these methods have rarely been applied in situ because of the requirements for a large amount of reagent, instruments, and a radioactive isotope. In addition, the proper storage of the sample (including urine and feces) on site until analysis is difficult. On the other hand, near infrared (NIR) spectroscopy requires no reagent and enables rapid measurement. In the present study, we attempted urinary NIR spectroscopy to determine the estrogen levels of orangutans in Japanese zoos and in the Danum Valley Conservation Area, Sabah, Malaysia. Reflectance NIR spectra were obtained from urine stored using a filter paper. Filter paper is easy to use to store dried urine, even in the wild. Urinary estrogen and creatinine concentrations measured by EIA were used as the reference data of partial least square (PLS) regression of urinary NIR spectra. High accuracies (R(2) > 0.68) were obtained in both estrogen and creatinine regression models. In addition, the PLS regressions in both standards showed higher accuracies (R(2) > 0.70). Therefore, the present study demonstrates that urinary NIR spectra have the potential to estimate the estrogen and creatinine concentrations.
Experiments demonstrated that visible and near-infrared (Vis-NIR) spectroscopy is a highly reliable tool for determining the nutritional status of plants. Although numerous studies on various kinds of plants have been conducted, there are only a few summaries of the research findings regarding the absorbance bands in the visible and near-infrared region and how they relate to the nutritional status of plants. This article will discuss the application of Vis-NIR spectroscopy for monitoring the nutrient conditions of plants, with a particular emphasis on three major components required by plants, namely nitrogen (N), phosphorus (P), and potassium (K), or NPK. Each section discussed different topics, for instance, the essential nutrients needed by plants, the application of Vis-NIR spectroscopy in nutrient status analysis, chemometrics tools, and absorbance bands related to the nutrient status, respectively. Deduction made concluded that factors affecting the plant's structure are contributed by several circumstances like the age of leaves, concentration of pigments, and water content. These factors are intertwined, strongly correlated, and can be observed in the visible and near-infrared regions. While the visible region is commonly utilised for nutritional analysis in plants, the literature review performed in this paper shows that the near-infrared region as well contains valuable information about the plant's nutritional status. A few wavelengths related to the direct estimation of nutrients in this review explained that information on nutrients can be linked with chlorophyll and water absorption bands such that N and P are the components of chlorophyll and protein; on the other hand, K exists in the form of cationic carbohydrates which are sensitive to water region.