The growth performance and survival rates of juvenile striped catfish, Pangasianodon hypophthalmus, reared at various levels of pH and temperature were described in this article. Two rearing trials were conducted separately for pH and temperature where both trials lasted for 35 days. One hundred and twenty juveniles (1.5 ± 0.23 g) were randomly stocked into 12 rectangle glass aquariums (n = 10 fish/tank; three replicates per treatment) with 100 L of water for each trial. The treatment consisted of four different pH level (7.5, 8.0, 8.5 and 9.0) and four different temperature level (26 °C, 28 °C, 30 °C and 32 °C). The survival of fish was counted at the end of experiments, and the weight of the juvenile was measured once a week. The quantification of feed intake was determined through the measurement of the residual weight of the feeds after the feeding process. Upon the conclusion of the experiment, the data pertaining to weight and feed intake were utilized to calculate the specific growth rate (SGR) and food conversion ratio (FCR) as indicators of growth performance. Additionally, the number of live fish was employed to ascertain the survival rate. The data obtained from the calculation of SGR, FCR and survival rate were next subjected to a normality test, one-way analysis of variance (ANOVA), and a Tukey post-hoc test. The information in this article will help in the business, experimental, and personal usage for P. hypophthalmus juveniles rearing process.