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Bacterial mediated silver nanoparticles and their efficacy against MRSA

Saleem S, Iqbal A, Hasnain S

Trop Biomed, 2020 Jun 01;37(2):482-488.
PMID: 33612817

Bacterial mediated Silver nanoparticles is considered as an emerging Ecofriendly approach to eradicate human pathogens. This paper aims to provide the biological approach for the synthesis of silver nanoparticles from indigenously isolated bacteria. This study will be beneficial to control the nosocomial infections triggered by MRSA (Methicillin-resistant Staphylococcus aureus). The current study is the extracellular synthesis of silver nanoparticles by using the cell free filtrate of bacterial strains isolated from the soil. The optimization study was also carried out to obtain the maximum production of silver nanoparticles. Nanoparticles were confirmed and characterized by UV-Vis spectroscopy and Transmission Electron Microscopy (TEM) having the plasmon resonance peak between 420-450nm with 10-60nm in size range and most were spherical in shape. Synthesized silver nanoparticles showed a potential antibacterial activity against MRSA (Methicillin Resistant Staphylococcus aureus) in-vitro study. This is the green approach for the production of AgNPs, as there was no previous work done on the synthesis of silver nanoparticles by bacteria in this region of Southern Punjab, Pakistan and these nanoparticles can be used to treat nosocomial infection. These silver nanoparticles can be used in effective disease management as antimicrobial agent.
The effect of peptidic and non-peptidic proteasome inhibitors on the biological properties of Acanthamoeba castellanii belonging to the T4 genotype

Siddiqui R, Saleem S, Khan NA

Exp Parasitol, 2016 Jun 18;168:16-24.
PMID: 27327524 DOI: 10.1016/j.exppara.2016.06.006

The treatment of Acanthamoeba infections remains problematic, suggesting that new targets and/or chemotherapeutic agents are needed. Bioassay-guided screening of drugs that are clinically-approved for non-communicable diseases against opportunistic eukaryotic pathogens is a viable strategy. With known targets and mode of action, such drugs can advance to clinical trials at a faster pace. Recently Bortezomib (proteasome inhibitor) has been approved by FDA in the treatment of multiple myeloma. As proteasomal pathways are well known regulators of a variety of eukaryotic cellular functions, the overall aim of the present study was to study the effects of peptidic and non-peptidic proteasome inhibitors on the biology and pathogenesis of Acanthamoeba castellanii of the T4 genotype, in vitro. Zymographic assays revealed that inhibition of proteasome had detrimental effects on the extracellular proteolytic activities of A. castellanii. Proteasome inhibition affected A. castellanii growth (using amoebistatic assays), but not viability of A. castellanii. Importantly, proteasome inhibitors affected encystation as determined by trophozoite transformation into the cyst form, as well as excystation, as determined by cyst transformation into the trophozoite form. The ability of proteasome inhibitor to block Acanthamoeba differentiation is significant, as it presents a major challenge in the successful treatment of Acanthamoeba infection. As these drugs are used clinically against non-communicable diseases, the findings reported here have the potential to be tested in a clinical setting against amoebic infections.
Fulltext The construction process for pre-stressed ultra high performance concrete communication tower

Lai VY, Hejazi F, Saleem S

PLoS One, 2020;15(11):e0238654.
PMID: 33147216 DOI: 10.1371/journal.pone.0238654

Towers are important structures for installing radio equipment to emit electromagnetic waves that allow radio, television and/or mobile communications to function. Feasibility, cost, and speed of the construction are considered in the design process as well as providing stability and functionality for the communication tower. This study proposes the new design for construction of segmental tubular section communication tower with ultra-high-performance fibre concrete (UHPFC) material and prestress tendon to gain durability, ductility, and strength. The proposed mix design for UHPFC in this study which used for construction of communication tower is consisted of densified Silica Fume, Silica fine and coarse Sand and hooked-ends Steel Fiber. The prestressed tendon is used in the tower body to provide sufficient strength against the lateral load. The proposed design allows the tower to be built with three precast segments that are connected using bolts and nuts. This paper presents a novel method of construction and installation of the communication tower. The advantages of proposed design and construction process include rapid casting of the precast segment for the tower and efficient installation of segments in the project. The use of UHPFC material with high strength and prestress tendon can reduce the size and thickness of the tower as well as the cost of construction. Notably, this material can also facilitate the construction and installation procedure.
Analysis of non-Newtonian magnetic Casson blood flow in an inclined stenosed artery using Caputo-Fabrizio fractional derivatives

Jamil DF, Saleem S, Roslan R, Al-Mubaddel FS, Rahimi-Gorji M, Issakhov A, et al.

Comput Methods Programs Biomed, 2021 May;203:106044.
PMID: 33756187 DOI: 10.1016/j.cmpb.2021.106044

BACKGROUND AND OBJECTIVE: Arterial diseases would lead to several serious disorders in the cardiovascular system such as atherosclerosis. These disorders are mainly caused by the presence of fatty deposits, cholesterol and lipoproteins inside blood vessel. This paper deals with the analysis of non-Newtonian magnetic blood flow in an inclined stenosed artery.
METHODS: The Casson fluid was used to model the blood that flows under the influences of uniformly distributed magnetic field and oscillating pressure gradient. The governing fractional differential equations were expressed using the Caputo Fabrizio fractional derivative without singular kernel.
RESULTS: The analytical solutions of velocities for non-Newtonian model were then calculated by means of Laplace and finite Hankel transforms. These velocities were then presented graphically. The result shows that the velocity increases with respect to Reynolds number and Casson parameter, while decreases when Hartmann number increases.
CONCLUSIONS: Casson blood was treated as the non-Newtonian fluid. The MHD blood flow was accelerated by pressure gradient. These findings are beneficial for studying atherosclerosis therapy, the diagnosis and therapeutic treatment of some medical problems.
Clinical Ethics in Pediatrics: An International Perspective

Lantos JD, Saleem S, Raza F, Syltern J, Khoo EJ, Iyengar A, et al.

J Clin Ethics, 2019 3 22;30(1):35-45.
PMID: 30896442

In this article, we first review the development of clinical ethics in pediatrics in the United States. We report that, over the last 40 years, most children's hospitals have ethics committees but that those committees are rarely consulted. We speculate that the reasons for the paucity of ethics consults might be because ethical dilemmas are aired in other venues. The role of the ethics consultant, then, might be to shape the institutional climate and create safe spaces for the discussion of difficult and sometimes contentious issues. Finally, we report how pediatric clinical ethics has evolved differently in a number of other countries around the world.
MALAT1: A key regulator in lung cancer pathogenesis and therapeutic targeting

Bhat AA, Afzal O, Afzal M, Gupta G, Thapa R, Ali H, et al.

Pathol Res Pract, 2024 Jan;253:154991.
PMID: 38070223 DOI: 10.1016/j.prp.2023.154991

Lung cancer remains a formidable global health burden, necessitating a comprehensive understanding of the underlying molecular mechanisms driving its progression. Recently, lncRNAs have become necessary controllers of various biological functions, including cancer development. MALAT1 has garnered significant attention due to its multifaceted role in lung cancer progression. Lung cancer, among other malignancies, upregulates MALAT1. Its overexpression has been associated with aggressive tumor behavior and poor patient prognosis. MALAT1 promotes cellular proliferation, epithelial-mesenchymal transition (EMT), and angiogenesis in lung cancer, collectively facilitating tumor growth and metastasis. Additionally, MALAT1 enhances cancer cell invasion by interacting with numerous signaling pathways. Furthermore, MALAT1 has been implicated in mediating drug resistance in lung cancer, contributing to the limited efficacy of conventional therapies. Recent advancements in molecular biology and high-throughput sequencing technologies have offered fresh perspectives into the regulatory networks of MALAT1 in lung cancer. It exerts its oncogenic effects by acting as a ceRNA to sponge microRNAs, thereby relieving their inhibitory effects on target genes. Moreover, MALAT1 also influences chromatin remodeling and post-translational modifications to modulate gene expression, further expanding its regulatory capabilities. This review sheds light on the multifaceted roles of MALAT1 in lung cancer progression, underscoring its potential as an innovative therapeutic target and diagnostic biomarker. Targeting MALAT1 alone or combined with existing therapies holds promise to mitigate lung cancer progression and improve patient outcomes.
The energetic brain - A review from students to students

Bordone MP, Salman MM, Titus HE, Amini E, Andersen JV, Chakraborti B, et al.

J Neurochem, 2019 10;151(2):139-165.
PMID: 31318452 DOI: 10.1111/jnc.14829

The past 20 years have resulted in unprecedented progress in understanding brain energy metabolism and its role in health and disease. In this review, which was initiated at the 14th International Society for Neurochemistry Advanced School, we address the basic concepts of brain energy metabolism and approach the question of why the brain has high energy expenditure. Our review illustrates that the vertebrate brain has a high need for energy because of the high number of neurons and the need to maintain a delicate interplay between energy metabolism, neurotransmission, and plasticity. Disturbances to the energetic balance, to mitochondria quality control or to glia-neuron metabolic interaction may lead to brain circuit malfunction or even severe disorders of the CNS. We cover neuronal energy consumption in neural transmission and basic ('housekeeping') cellular processes. Additionally, we describe the most common (glucose) and alternative sources of energy namely glutamate, lactate, ketone bodies, and medium chain fatty acids. We discuss the multifaceted role of non-neuronal cells in the transport of energy substrates from circulation (pericytes and astrocytes) and in the supply (astrocytes and microglia) and usage of different energy fuels. Finally, we address pathological consequences of disrupted energy homeostasis in the CNS.