Active packaging is becoming increasingly significant in the food industry. The present study aims to explore the use of Syzygium Cumini Seed Extract (SCSE) as an antioxidant and chitosan as an antibacterial agent to produce active packaging based on polylactic acid (PLA), poly ε-caprolactone (PCL), and polyethylene glycol (PEG) blend. Using advanced characterization techniques, active packaging (PLA/PCL/PEG) incorporating with 0.5 g chitosan-0.5 mL SCSE was evaluated for its mechanical, physical, structural, and antibacterial-antioxidant properties. The addition of chitosan-SCSE caused an 18.57 % increase in tensile strength and decreased the Water Vapor Transmission Rate (WVTR) by up to 52 %, whereas smooth surface microscopy indicated good compatibility between polymers and active agents. Active packaging incorporating chitosan-SCSE reduced 96.66 % of Gram-positive bacteria Staphylococcus aureus and 73.98 % of Gram-negative bacteria, Escherichia coli. During 15 days of storage, the active packaging was able to slow the increase in Total Volatile Basic Nitrogen (TVBN) in beef and prevent the decrease in vitamin C contents in pineapple.
Broiler chicken (Gallus gallus) is a source of animal protein with a high nutritional content. The purpose of this study was to evaluate the quality of broiler chicken meat (Gallus gallus) by analyzing its nutritional value, genetic profile, and protein level. The chicken meat samples were obtained from four different districts in Malang city, Indonesia. We analysed the proximate composition of chicken meat to detect its nutrition content. Furthermore, we have examined the sequence of the Myoz1 gene as well as the level of ApoB proteins in the same meat. The nutritional analysis of chicken meat showed that in the four locations different levels of protein, ash, water, and lipids were observed. The Myoz1 gene of femur chicken broilers from the second and third districts has five and twenty-one substitution mutations, respectively. The ApoB expression level in locations 2 and 3 was higher than that in the other districts. In conclusion, Myoz1 and ApoB levels were correlated with the nutritional content and quality of broiler chicken meat.
This study aims to produce and investigate the potential of biodegradable Polylactic Acid (PLA)-based composites mixed with chitosan and Turmeric Essential Oil (TEO) as an anti-microbial biomaterial. PLA has good barrier properties for moisture, so it is suitable for use as a raw material for making packaging and is included in the GRAS (Generally Recognized As Safe). Chitosan is a non-toxic and antibacterial cationic polysaccharide that needs to be improved in its ability to fight microbes. TEO must be added to increase antibacterial properties due to a large number of hydroxyl (-OH) and carbonyl functional groups. The samples were prepared in three different variations: 2 g of chitosan, 0 mL TEO and 0 mL glycerol (Biofilm 1), 3 g of chitosan, 0.3 mL TEO and 0.5 mL of glycerol (Biofilm 2), and 4 g of chitosan, 0.3 of TEO and 0.5 mL of glycerol (Biofilm 3). The final product was characterized by its functional group through Fourier transform infrared (FTIR); the functional groups contained by the addition of TEO are C-H, C=O, O-H, and N-H with the extraction method, and as indicated by the emergence of a wide band at 3503 cm-1, turmeric essential oil interacts with the polymer matrix by creating intermolecular hydrogen bonds between their terminal hydroxyl group and the carbonyl groups of the ester moieties of both PLA and Chitosan. Thermogravimetric analysis (TGA) of PLA as biofilms, the maximum temperature of a biofilm was observed at 315.74 °C in the variation of 4 g chitosan, 0.3 mL TEO, and 0.5 mL glycerol (Biofilm 3). Morphological conditions analyzed under scanning electron microscopy (SEM) showed that the addition of TEO inside the chitosan interlayer bound chitosan molecules to produce solid particles. Chitosan and TEO showed increased anti-bacterial activity in the anti-microbial test. Furthermore, after 12 days of exposure to open areas, the biofilms generated were able to resist S. aureus and E. coli bacteria.