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  1. Sumardino, Widodo, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:228-233.
    PMID: 32713577 DOI: 10.1016/j.enfcli.2019.11.061
    INTRODUCTION: Cases of trauma still becomes a health problem in almost all countries causing death in few cases. In some developing countries, the insidences of head injury tend to increase. One of the reasons for the many death and disability may be due to the inavailability and inadequacy of pre-hospital first aid.

    AIM: The general objective of this study is to find out the description of community first responder in providing pre-hospital first aid to head injuries.

    METHODS: This study uses qualitative descriptive method.

    RESULTS: Most of the respondents have variety of educational backgrounds and do not have sufficient knowledge and skills to provide first aid. The average respondents provided help by performing initial assessment, managing effective airway and controlling bleeding. Limited pre-hospital facilities become one of the reasons for respondent not getting help so the efforts provided are not maximal. Respondents prefer to send patients directly to health facilities.

    CONCLUSION: Regular education and training programs for the community first responders should be initiated so that the number of death and disability can be minimized.

  2. Poddar, S., Hati, A.K., Pal, D., Bhattacharya, N., Mukim, Y.
    JUMMEC, 2018;21(2):10-14.
    MyJurnal
    Purpose: The object of this study was to identify patients with diagnosed dengue infection, who were positive
    for both dengue-specific NS1 antigen and IgM antibody.
    Method: From January 2013 to December 2016, in Central Kolkata in West Bengal in India, patients with
    symptoms of dengue infection, were sent to the laboratory by the physicians for confirmatory diagnosis of
    dengue infection. A total of 4762 patients were seen, and serum samples tested and distributed into seven
    panels, according to the investigations requested. 1436 patients were tested positive.
    Results: 1053 cases were tested for both NS1 and Ig M antibody, 835 for dengue-specific NS1 antigen, IgM and
    IgG antibodies and 218 for NS1 dengue-specific antigen and IgM antibody. Of these, dengue was confirmed in
    34.3 %, with 16.6% positive for both NS1 antigen and IgM antibody. Eleven were diagnosed, with late dengue
    infection, thirty-nine with late primary infections and ten with late secondary dengue infection.
    Conclusions: Many of the patients were reactive for both NS1 antigen and IgM antibody, and they required
    proper attention and strict vigilance with effective monitoring and treatment, not of early dengue infection,
    but of late dengue infection. Unless the serological tests for Ig M and IgG antibodies, and the dengue specific
    viral antigen NS1 are performed simultaneously, these types of cases would not all be detected.
  3. Bhattacharya S, Basu P, Poddar S
    J Prev Med Hyg, 2020 Jun;61(2):E130-E136.
    PMID: 32802995 DOI: 10.15167/2421-4248/jpmh2020.61.2.1541
    SARS-CoV-2 is a new form of β-coronavirus that has been recently discovered and is responsible for COVID 19 pandemic. The earliest infection can be traced back to Wuhan, China. From there it has spread all over the world. Keeping in view the above perspective, an attempt is made in order to find out the epidemiological pattern of COVID 19 pandemic, if any, in different geo-climatological regions of the world in terms of case incidence and mortality. This study is also an endeavor to review and analyze the gradual changes of the genetic makeup of SARS-CoV from evolutionary and epidemiological perspectives. The raw data of COVID-19 cases and death incidences were collected from the World Health Organization (WHO) website from the time period: 1st April to 6th April, 2020. The data that are utilized here for general and Case fatality rate (CFR) based analysis. Western pacific region, European region and Americas have the greatest number of infected cases (P < 0.001); whereas deaths have been found to be significantly higher in Europe (P < 0.001). Total number of confirmed cases and deaths in south-east Asia are comparatively lower (P < 0.001). Case fatality rate (CFR) has also found significant for European region. SARS-CoV-2 is considered to be a strain of SARS-CoV that has a high rate of pathogenicity and transmissibility. Result indicated that the European region has been affected mostly for both cases and death incidences. The novel mutations in SARS-CoV-2 possibly increase the virus infectivity. Genetic heterogeneity of this virus within the human population might originate as the representatives of naturally selected virus quasispecies. In this context, the presence of the asymptomatic individuals could be a significant concern for SARS-CoV-2 epidemiology. Further studies are required to understand its genetic evolution and epidemiological significance.
  4. Aristoteles, Rini PS, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:1-5.
    PMID: 32713543 DOI: 10.1016/j.enfcli.2019.11.014
    BACKGROUND: Communication in adolesencest is an interpersonal interaction that use symbolic linguistic system, such as a system of verbal and non-verbal symbols. Factors that influence adolescent communication include environmental factors, education, and online games.

    RESEARCH OBJECTIVES: To find out the correlation between frequency of playing online games and teen communication on students' Level I & II of STIKes Muhammadiyah Palembang.

    RESEARCH METHOD: the research method use was quantitative descriptive research with Cross Sectional approach using Purposive Sampling as the technique of collecting data with a simple of 262 out of 760 populations. The instrument used in the form of a questionnaire about the frequency of playing online games and teen communication made by the researcher and has been tested for validity and reliability. This research was conducted in March-April 2019.

    RESULT: From the results of the study, most of the respondents with 116 respondents (82.3%) had passive communication with the frequency of playing online games often, and a small number of respondents with 2 respondents (2.9%) had aggressive communication with the frequency of playing online games sometimes. The result test of Chi Square was obtained a value P value=0.000 (<0.05) CONCLUSION: There was a correlation between frequency of playing online games and teen communication on students' level I & II STIKes Muhammadiyah Palembang.

  5. Sadiman S, Islamiyati I, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:115-118.
    PMID: 32713547 DOI: 10.1016/j.enfcli.2019.11.035
    The purpose of this study is to determine whether there are differences in hemoglobin levels before and after consuming ambon bananas in students of Prodi Kebidanan Metro. The specific purpose of this study was to determine hemoglobin levels before consuming ambon bananas, hemoglobin levels after consuming ambon bananas and the differences in hemoglobin levels before and after consuming ambon bananas in female students at Prodi Kebidanan Metro. This research is an intervention research with quasi experiment design with The One Group Pretest - posttest design. The population in this study were students in the first and second grade in Prodi Kebidanan Metro who lived in the dormitory as many as 149 female students. A sample of 49 people will take a portion of the population using simple random sampling technique. Data analysis used dependent sample t tests to test the mean increase in Hb levels before and after consuming ambon bananas. The results showed an average Hb level before consuming bananas at 12.51g/dl and an the average hb level after consuming bananas was 12.89g/dl, so the average increase in Hb levels was 0.39g/dl. The results of the analysis using the t-test dependent test obtained p value=0.000, which means that there are significant differences in Hb levels of students before and after consuming ambon banana.
  6. Eryando T, Sipahutar T, Poddar S
    F1000Res, 2023;12:966.
    PMID: 39246585 DOI: 10.12688/f1000research.135262.2
    BACKGROUND: This study investigated the determinants of community preventive behavior in complying with the Indonesian regulations to prevent COVID-19 local transmission.

    METHODS: A cross-sectional study used to collect the data via an online cross using a form created from a google questionnaire forms. A total of 1,802 respondents were gathered at a single point in time. The authors used the Health Belief Model (HBM) approach to measure and create a model for the prevention of local transmission of COVID-19.

    RESULTS: This study found that more than half of the respondents still had low perceived susceptibility (16%) and severity (43%). There were only 3% respondents with perceived barriers and 19% with strong self-efficacy. The findings showed that self-efficacy and perceived barriers had statistically significant relationships with preventive behavior (p-value <0.05). The goodness of fit index showed that the proposed model was not fit for the data (RMSE<0.080, GFI>0.950, AGFI>0.950, SRMR<0.100), which means that it was not fit to describe the empirical phenomenon under study.

    CONCLUSIONS: This study found that more than half of the respondents still had low perceived susceptibility (84%) and severity (67%), but more than half had high perceived benefits (54%). Only a few respondents had significant barriers to implementing COVID-19 transmission prevention behaviours (3%). Still, most respondents had low perceived self-efficacy (81%), and only 60% had good behaviours related to COVID-19 prevention. In the context of COVID-19 preventive behaviour, we recommended to improve perceived susceptibility and severity by providing the correct information (which contain information about how people susceptible to the virus and the impact of infected by the virus) with the local cultural context.

  7. Pangaribuan IK, Simanullang E, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:92-95.
    PMID: 32713594 DOI: 10.1016/j.enfcli.2019.11.029
    The toddler stage is a golden period and at the same time a critical period for a child, since he / she will experience rapid brain growth and development so that he/ she need sufficient nutrition intake. Toddler's abnormal growth and development are usually found in low economic community because they cannot afford to provide their need for basic food for their toddler's growth and development. The objective of the research was to analyze toddler's growth and development according to family's economic status. The research used descriptive analytic method with cross-sectional design. It was conducted at Limau Manis Village, Tanjung Morawa Subdistrict, Deli Serdang Regency, from January to June 2016. The population was 1899 toddlers, and 113 of them were used as the samples, taken by using simple random sampling technique. The data were gathered by using questionnaires to find out toddler's growth and development according to family's economic status. The result of univariate analysis showed that 57 toddlers (50.4%) had abnormal growth and development. The result of bivariate analysis with chi-square test showed that there was the correlation of toddler's growth and development with economic status (p value=0.004), women's care pattern (p value=0.028), and environmental sanitation (p value=0.01). Therefore, empowerment of family's economy through the development of family's nutrition portfolio is necessary.
  8. Syapitri H, Hutajulu J, Poddar S, Bhaumik A
    Enferm Clin, 2020 06;30 Suppl 5:183-187.
    PMID: 32713565 DOI: 10.1016/j.enfcli.2019.11.051
    The purpose of this study was to analyze the adaptation response after the eruption of Mount Sinabung in Gurukinayan Village, Karo District. This research is qualitative with an explorative phenomenological approach. Data collection was carried out through observation and in-depth interviews with key informants who were the victims of the Mount Sinabung eruption. The analysis was conducted using content analysis description and life history with 6 participants. The adaptation response results obtained from the community were maladaptive (staying/surviving in the eruption site). The impacts of this eruption are physical/health impacts (cough, shortness, flu and fever), psychological impacts (trauma, anxiety and panic), social/economic impacts (crop failure and job loss), and infrastructure impacts (damaged houses, damaged roads and clean water crisis). Post-eruption adaptation strategies are from the aspects of health (medical treatment, traditional medicine mix), social/economic aspects (carrying out community activities, cultivating land and expecting food and land assistance from donors), infrastructure aspects (building huts, repairing houses, clean water treatment, and expecting operational assistance from the government). It is recommended that in handling post-eruption of Mount Sinabung, the government or village apparatus establish a post-disaster recovery program and decision making (stakeholders) in making policies or decisions related to Eruption Disasters handling.
  9. Sarli D, Gunawan I, Novinaldi, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:129-132.
    PMID: 32713551 DOI: 10.1016/j.enfcli.2019.11.038
    Postpartum depression (PPD) is quite large, where there are 1 in 25 postpartum women experiencing PPD who still report symptoms of PPD after six months. The highest risk of experiencing PPD is more experienced by primiparas detected in 10-19 postpartum days. For PPD to not adversely affect the mother and baby, initial screening is needed to prevent the occurrence of PPD by using an Android-based EPDS application. The research objectives are an early screening of baby blues based on Android application and to determine the factors influence of baby blues. Participants download EPDS apps to make it easier for participants to screen the baby blues at the beginning of the first week after giving birth. On fourth week, the mothers refill EPDS apps screening to compare the results of screening the first week with fourth week using the Dependent T-test. In this study also analyzed the factors that influence the baby blues such as education, employment, parity, and age using the ANOVA Test. The study sample was the first-week postpartum mothers with a total sample of 64 people. The average EPDS screening results in the first week were 6.64, with a standard deviation of 2.57. The screening results on fourth week are 6.70, with a standard deviation of 2.53. The results of statistical tests obtained p-value 0.208; it can conclude that there was no difference in the results of screening tests in the first week with the fourth week. PPD events occur mostly in primiparas and women aged <20 years with p-value 0.001, while in education p-value 0.596 and employment-value 0.784. It recommended for pregnant women and health workers to do screening in the first week of postpartum so that it can detect PPD early.
  10. Surya M, Zuriati Z, Zahlimar, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:171-174.
    PMID: 32713562 DOI: 10.1016/j.enfcli.2020.02.001
    OBJECTIVE: Pain management is one of the methods used in the health sector. Nursing care focuses on non-pharmacological measures, including the provision of aromatherapy using lavender and rose aromatherapy, which aims to understand the effectiveness of lavender and rose aromatherapy on pain intensity of postoperative patients in Laparatomi Hospital in Aisyiyah General Hospital in the surgical inpatient room, Padang in 2019.

    METHOD: The design of this study is a Quasi Experiment with pre-test and post-test designs with two groups. The sample of this study was Post Laparatomi in RSU with the technique of Accidental Sampling. The sample of this study consisted of 30 people for lavender and rose aromatherapy. Statistical tests used the Independent Test T-test.

    RESULT: Based on the results of the study it was found that lavender and rose aromatherapy can reduce pain scale in postoperative patients. But compared to the two intervention groups, the aroma group of lavender therapy is more effective with mean pain scale before lavender aromatherapy being 5.20 and pre-test is 3.40 with p-value 0.000.

    CONCLUSION: The nurses could provide nursing care in dealing with pain management in postoperative patients in addition to deep breathing techniques. It is recommended to apply aroma lavender therapy, which reduces pain and makes patients more relaxed.

  11. Elba F, Hilmanto D, Poddar S
    J Public Health Res, 2022 Oct;11(4):22799036221139939.
    PMID: 36466154 DOI: 10.1177/22799036221139939
    INTRODUCTION: There has been an increase in the usage of herbal remedies, particularly among pregnant women, across the globe. The prevalence of herbal medicine use during pregnancy ranged from 54.3% to 96.6% in the region studied, indicating a rising trend in the use of herbal medicine during pregnancy. Cold and flu symptoms and stomach problems were the most prevalent reasons people turned to this drug.

    MATERIALS AND METHODS: This study's samples were pregnant women in the working area of Ciwaruga Public Health Center, which has eight villages. The samples were taken using a proportional simple random sampling technique until 96 respondents were obtained. The percentage of moms who took herbal medication was the primary outcome variable, and participants were chosen for the research using a systematic sampling procedure. A digital database was used to store quantitative data that had been coded.

    RESULTS: The factors significantly associated with the use of herbal medicines during pregnancy were perception (OR 2.18; CI 1.02-4.66), having ever used herbal medicines during a previous pregnancy (OR 3.87; CI 1.46-10.25), and healthy reproductive is other reasons (OR 7.98; CI 4.45-14.30).

    CONCLUSION: Pregnant women in the Ciwaruga Public Health Center area often turn to herbal remedies, which may indicate a lack of access to mainstream medical treatment. They believe that herbal medications are useful and that using them during a prior pregnancy is linked.

  12. Ikhlasiah M, Winarni LM, Poddar S, Bhaumik A
    Enferm Clin, 2020 06;30 Suppl 5:202-205.
    PMID: 32713569 DOI: 10.1016/j.enfcli.2019.11.054
    Based on preliminary studies, it is known that 80% of working mothers fail to make exclusive breastfeeding, on average babies are given formula milk 2-4 months old and stop breastfeeding at 6-18 months of age. Papaya leaves are one of the galactagogues that contain quercetin which can activate the hormone prolactin and help increase breastmilk. The purpose of this study was to determine the effect of giving papaya leaf juice for nursing mothers who work toward increasing levels of the hormone prolactin and infant weight in Tangerang. The research design used was a pre-experimental model with one group pretest-posttest design. Samples taken as many as 10 mothers who have babies aged 0-6 months who have difficulty in breastfeeding because the amount of breastmilk was a little. The study was conducted in several clinics in Tangerang. The intervention was giving papaya leaf juice for 7 consecutive days. The results obtained from this study are as follows: 90% of mothers aged between 20 and 35 years, 70% of mothers have a history of spontaneous labor and have no complications at the time of delivery, 40% of mother's education is junior high school (SMP), and 70% of mothers are primiparas. The average increase in the amount of prolactin after the intervention was 19.59ng/ml, while the average weight gain of the newborn after the intervention was 165g. Wilcoxon test results for increased levels of the hormone prolactin p-value of 0.047<0.05 and for an increase in body weight of newborns p-value of 0.009<0.05. CONCLUSION: There is an effect of giving papaya leaf juice for nursing mothers who work on increasing levels of the hormone prolactin and infant weight in Tangerang. Papaya leaf juice can be a galactagogue for mothers who experience problems with a small amount of breastmilk.
  13. John N, Rai RP, Amidi A, Poddar S
    Enferm Clin, 2020 06;30 Suppl 5:188-191.
    PMID: 32713566 DOI: 10.1016/j.enfcli.2020.02.005
    OBJECTIVE: The purpose of this study was to identify the nurse's overall experience with a mentoring programme and the study specifically seeks to determine the attitudes and perceived benefits of students in different semesters regarding the mentoring programme.

    METHOD: The research design was quantitative. Face-to-face survey method based on a given questionnaire was conducted with all the targeted respondents. The methodology used was a non-experimental descriptive research design. Total of 146 respondents out of 243 populations were selected using a stratified random sampling strategy to determine the research sample to give equal opportunity.

    RESULTS: Findings of hypotheses test using one-way ANOVA indicated that there is a significant difference in attitudes towards the mentoring programme and perceived benefits between different groups of nursing at Training Institute Ministry of Health (Nursing) Sandakan.

    CONCLUSION: In general, the mentoring programme had a positive impact. However, the level of attitude and perceived benefit is different among students in different semesters. The overall mean result is good and reflecting nursing students are fairly benefited from the mentoring programme.

  14. Sya'diyah H, Efendi F, Mahmudah, Saidah QI, Poddar S
    J Public Health Res, 2023 Jul;12(3):22799036231197172.
    PMID: 37667680 DOI: 10.1177/22799036231197172
    BACKGROUND: The elderly with dementia occur cognitive decline and they are considered normal by the family, and then causing the need for care from elderly. This is not following the ability of home care by the family as an informal caregiver for the elderly with dementia. The purpose of this study was to analyze the effect of caregiver demands on the ability of families to do home care for elderly dementia.

    DESIGN AND METHODS: An analytical observational study with a cross-sectional design. The sample size is 100 respondents with a cluster random sampling technique. Data were collected by questionnaire and analyzed using SEM-PLS.

    RESULTS: Caregiver demands have a direct effect on increasing the family's ability to do home care for elderly dementia with a p-value = 0.011 (p ≤ 0.05), t statistic value of 2.557 (≥1.96). Caregiver demands have four indicators, including care receiver impairment, caregiving activities, competency of caregivers, and caregiver/family relationship with elderly dementia. The condition of the elderly who experience various changes supports the increasing need for care to receive care and recovery as well as special attention from the family.

    CONCLUSION: The higher the need for care, the higher the family's ability to do home care. This research implies that the family has a duty in the health sector to provide care for the elderly at home/home care, which strengthens the need for care, this supports caregiver empowerment and increases the independence of the elderly with dementia.

  15. Abdullah D, Poddar S, Rai RP, Purwati E, Dewi NP, Pratama YE
    J Public Health Res, 2021 Nov 24;10(s2).
    PMID: 34818881 DOI: 10.4081/jphr.2021.2508
    BACKGROUND: Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for ±2 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB), which has the potential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban, Kab. Fifty Cities, West Sumatra.

    DESIGN AND METHODS: A survey method was used in this research with a descriptive analysis, Antimicrobial activity testing was done with bacteria Escherichia coli O157, Staphylococcus aureus, Listeria monocytogenes, and Listeria innocua. Molecular identification was done using the 16S rRNA gene.

    RESULTS: Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zone area results was obtained in 2DA isolates with Escherichia coli O157 test bacteria of 21.16 mm, Staphylococcus aureus with a clear zone area of 23.17 mm, Listeria innocua of 19.24 mm and Listeria monocytogenes with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running PCR base length 1419bp.

    CONCLUSIONS: Phylogenetic analysis shows that Dadiah of Limapuluh Kota Regency is a kin to Lactobacillus plantarum. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand.

  16. Manik H, Triyoga RS, Siregar MFG, Rochadi RK, Poddar S
    J Public Health Res, 2021 Dec 28;10(s2).
    PMID: 34964570 DOI: 10.4081/jphr.2021.2707
    BACKGROUND: Health and mortality problems are closely related to the maternal mortality rate (MMR). Efforts to reduce MMR have been carried out by many countries, including the Indonesian government.

    DESIGN AND METHODS: This research was conducted using two approaches, namely quantitative and qualitative or mixed methods and 149 respondents and 26 informants, to reduce MMR in Dairi Regency. This study was also carried out to determine the dominant variable that affects mother's behavior in an effort to reduce MMR in accordance with the existing theory.

    RESULT: Mother's intention to contribute to the reduction of MMR in this study was influenced by the good factor directly or indirectly. This is indicated by the score p<0.005. The study also shows that it is very important for the health workers to be able to communicate well with individuals and communities.

    CONCLUSION: The present study will help to reduce maternal fatalities, and will help to build systems and processes that will allow control the behaviour of the pregnant women and determine the cause of death as well as its contributing factors.

  17. Ambarika R, Said MSM, Adiutama NM, Anggraini NA, Poddar S, Abdullah BF
    Enferm Clin (Engl Ed), 2024 Apr 17.
    PMID: 38641005 DOI: 10.1016/j.enfcle.2024.04.004
    INTRODUCTION: The average stroke morbidity rate, especially in rural communities, is higher when compared to the national average.

    OBJECTIVE: Identify the profile of knowledge and awareness of stroke in rural and urban communities and analyze the differences between the two.

    METHOD: Respondents who met the requirements answered a questionnaire containing a total of 41 items regarding knowledge and awareness of stroke. Characteristic data obtained includes age, gender, education, occupation, and ethnicity. Differences in characteristics were analyzed using Mann-Whitney, and the profiles of knowledge and awareness of stroke in rural and urban groups were analyzed using independent t tests to see differences between the two.

    RESULT: Differences in the characteristics of rural and urban groups were only in education (p = 0.036) and occupation (p = 0.021). The mean score of the knowledge domain for the rural group was 72.53 (SD = 11.04), for the urban group it was 81.06 (SD = 11.12). The mean score awareness of stroke domain in the rural group was 72.18 (SD = 11.01), the urban group was 85.51 (SD = 11.67). The profile of knowledge and awareness of stroke in rural communities was significantly lower than in urban communities. The significance value was (p = 0.004) for knowledge and (p = 0.002) for awareness of stroke.

    CONCLUSION: Based on the findings, it is reasonable to suspect that educational and occupational factors contribute to differences in the profile of knowledge and awareness of stroke in the two groups. Therefore, the stroke awareness campaign strategy for rural communities needs to be specifically designed by emphasizing/considering educational and occupational factors.

  18. Bharadwaj KK, Rabha B, Ahmad I, Mathew SP, Bhattacharjee CK, Jaganathan BG, et al.
    J Biomol Struct Dyn, 2023 Nov 28.
    PMID: 38014451 DOI: 10.1080/07391102.2023.2275187
    Overexpression of HDAC 2 promotes cell proliferation in ovarian cancer. HDAC 2 is involved in chromatin remodeling, transcriptional repression, and the formation of condensed chromatin structures. Targeting HDAC 2 presents a promising therapeutic approach for correcting cancer-associated epigenetic abnormalities. Consequently, HDAC 2 inhibitors have evolved as an attractive class of anti-cancer agents. This work intended to investigate the anti-cancer abilities and underlying molecular mechanisms of Rhamnetin in human epithelial ovarian carcinoma cells (SKOV3), which remain largely unexplored. We employed various in vitro methods, including MTT, apoptosis study, cell cycle analysis, fluorescence microscopy imaging, and in vitro enzymatic HDAC 2 protein inhibition, to examine the chemotherapeutic sensitivity of Rhamnetin in SKOV3 cells. Additionally, we conducted in silico studies using molecular docking, MD simulation, MM-GBSA, DFT, and pharmacokinetic analysis to investigate the binding interaction mechanism within Rhamnetin and HDAC 2, alongside the compound's prospective as a lead candidate. The in vitro assay confirmed the cytotoxic effects of Rhamnetin on SKOV3 cells, through its inhibition of HDAC 2 activity. Rhamnetin, a nutraceutical flavonoid, halted at the G1 phase of the cell cycle and triggered apoptosis in SKOV3 cells. Furthermore, computational studies provided additional evidence of its stable binding to the HDAC 2 protein's binding site cavity. Based on our findings, we conclude that Rhamnetin effectively promotes apoptosis and mitigates the proliferation of SKOV3 cells through HDAC 2 inhibition. These results highlight Rhamnetin as a potential lead compound, opening a new therapeutic strategy for human epithelial ovarian cancer.Communicated by Ramaswamy H. Sarma.
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