Length-weight relationship (LWR) was used as a tool to assess the status of fish stocks, through condition factor, in major rivers in Thailand. Fifty-one fish species from each river, i.e., The examined for LWR using 11 years of monitoring data (2010-2020) for Bang Pakong and 14 years of monitoring data (2007-2020) for Chao Phraya, which comprised 57,871 samples. The parameters for LWR and condition factor were examined by species and by body shape, i.e., ovate, oblong, elongate or eel-like. The coefficient of determination (r 2 ) of all log-transformed LWRs was greater than 0.90. Parameter b of LWR ranged between 2.06 and 3.46 (median = 3.00) for fishes from the Chao Phraya River and between 1.72 and 3.68 (median = 2.76) for fishes from the Bang Pakong River. The overall condition factor, which implies the well-being that indicates the health or fattening of the fishes in a stock, ranged between 0.93 and 1.09. There was no significant difference in the overall well-being (P = 0.279) between the two rivers. Fishes with oblong and elongate shapes in the Chao Phraya River showed higher median values of parameter b of LWR than those from the Bang Pakong River. However, there was no significant difference (P > 0.05) in the well-being of the fish stocks between the two rivers when pooled by shape. The findings are fundamental information for fish stock assessment in the two rivers, which greatly support the small-scale fisheries in Thailand.