Diuron has been substantially used in different parts of the world as an herbicide and as a bioactive ingredient in antifouling paint. However, knowledge on the toxicity of Diuron that could adversely affect non-targeted aquatic organisms, especially fish; is still deficient. Therefore, this study was conducted to determine the possible sublethal effects of phenylurea herbicide Diuron at the cellular level of the liver and kidneys of an adult Javanese medaka (Oryzias javanicus), which has been used as the novel model organism. In the present study, histological assessment was carried out using the hematoxylin and eosin (H&E) staining protocol where the samples were preserved in 10% buffered formalin and sectioned into 5 μm thickness. The 210 samples used contained a range of Diuron concentrations comprising 1 μg/L, 50μg/L, 100 μg/L, 500 μg/L and 1000 μg/L; which also included the control treatments. Semi quantitative analysis revealed that liver and kidney tissues exposed at higher concentrations (500 μg/L and 1000 μg/L) showed significantly moderate to severe deformities and mild lesion alterations were already shown even at a lower concentration of Diuron exposure (1.0 μg/L), as compared to the control treatment of dechlorinated water (p
The present study aims to determine the level of heavy metals in surface soils and street dust from selected urban and peri-urban locations in the metropolitan city of Kuala Lumpur. Samples were collected from 15 different locations, where Kuala Lumpur City Centre (KLCC) served as the centre point while other locations were located at specific distances from the centre. Surface soils and street dust were collected to detect the level of contamination based on five elements (Zn, Cu, Pb, Cd and Fe). Results indicated that the metal distribution displayed a descending trend as follows: [Fe] > [Zn] > [Pb] > [Cu] > [Cd] and [Fe] > [Zn] > [Cu] > [Pb] > [Cd] in the surface soil and street dust samples, respectively. Geoaccumulation index (Igeo), contamination factor (CF) and pollution load index (PLI) were calculated. Based on the Igeo index, Fe in both samples was found to cause no pollution in all study sites. In surface soils, CF showed all sites were contaminated with Cd in both soil and street dust. In conclusion, the soil sample was polluted with metals while no pollution was found in the street dust in all sites sampled. Further intensive studies are recommended in order to determine other factors which may cause contamination.