Introduction: Traditionally, Mallotus paniculatus (Balik Angin) plant is used in the treatment of various
diseases in rural areas such as remedy after childbirth, wound healing and fever. In this present study, four
medicinal properties of the plant were investigated which included antibacterial, antifungal, anticancer and
antioxidant activities. Materials and Methods: Potential medicinal compounds were extracted from the plant
leaves by sonication with 3 different solvents namely ethanol, ethyl acetate and hexane respectively. The
antibacterial and antifungal properties were determined using disc diffusion agar and broth dilution assay,
the antioxidant activity by DPPH scavenging assay and the anticancer effect by MTT assay. Results: From the
screening of the medicinal properties, M. paniculatus leave extracts were shown to possess antibacterial,
antioxidant and anticancer properties but not antifungal properties. Ethanolic and ethyl acetate extracts of
the leave were active against gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) but not
gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The antioxidant activity of the
ethanolic crude extract was high; with IC50 of 30 μg/ml comparable with the positive controls; ascorbic acid
and butylated hydroxytoluene (BHT). Both ethanolic and ethyl acetate extracts were cytotoxic against breast
cancer (MCF7), colon cancer (HT-29), cervix cancer (Hela) cell lines. Conclusion: M. paniculatus leave
extract has many potential medicinal values for further studies.