Due to high energy demand worldwide, finding an alternative renewable and
sustainable energy source is of great interest. Plant microbial fuel cell (P-MFC) is one
of the most promising methods to generate green energy. In P-MFC, a plant is placed
into the anode compartment. Mutual interaction between plant root rhizodeposits
and bacterial community results in the biofilm formation at the vicinity of the
rhizosphere area in plant root could be utilized to generate electricity. Indeed, in PMFC,
bacteria metabolize rhizodeposits into electrons and protons. These electrons
could be then converted into green electricity. The objectives of this research are to
utilize Epipremnum aureum plant collected from Kota Tinggi’s lake to generate
electricity and observe current generation by different resistors, to characterize
immobilized bacteria attached on the anode surface then identify the optimum growth
temperature for isolated bacteria. Five plant microbial fuel cells were constructed in a
H-shape (dual- chambers) configuration in the plastic container. Maximum current
density for 20 days for P-MFC by external resistance of 100k Ω was 0.1 µA/cm2
with
maximum power density of 0.85 µW/cm2 and the open circuit voltage (OCV) was
measured at 195 mV. Besides, fresh biomass averages increased 5g after 20 days of
experiments below and above ground as compared to the initial fresh biomass. Five
isolated bacterial strains from the graphite felt surface found on the anode were
screened by nine biochemical tests such as catalase, TSI (triple sugar iron agar), gelatin
and etc. The immobilized bacteria attached to anode electrode in P-MFC were further
examined with Fast Electron Scanning Electron Microscopy (FESEM). The isolated
bacterial growth curves were determined at two different temperatures of 25 °C and
37 °C. The optimum growth temperature predominantly for them was 37 °C.