Partial differential equations involve results of unknown functions when there are multiple independent variables. There is a need for analytical solutions to ensure partial differential equations could be solved accurately. Thus, these partial differential equations could be solved using the right initial and boundaries conditions. In this light, boundary conditions depend on the general solution; the partial differential equations should present particular solutions when paired with varied boundary conditions. This study analysed the use of variable separation to provide an analytical solution of the homogeneous, one-dimensional heat equation. This study is applied to varied boundary conditions to examine the flow attributes of the heat equation. The solution is verified through different boundary conditions: Dirichlet, Neumann, and mixed-insulated boundary conditions. the initial value was kept constant despite the varied boundary conditions. There are two significant findings in this study. First, the temperature profile changes are influenced by the boundary conditions, and that the boundary conditions are dependent on the heat equation’s flow attributes.