Prazosin (PRZ), a drug used to treat hypertensive patients, is an emergent contaminant in water systems. PRZ is an alpha-adrenergic receptor blocker used to treat anxiety, and is believed to reach the environment through human excretion, irresponsible disposal of unused medicine, and waste products from manufacturing plants. The purpose of this research was to isolate and characterize potential microbes for PRZ biodegradation and to identify the degradation pathway. After screening, isolated strain STP3 showed a capability for PRZ degradation and was chosen for further analysis. Resting cell assays with PRZ were conducted to identify the intermediate metabolites formed from biodegradation by Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) analysis. Two metabolites degraded from PRZ by STP3 were successfully found, and as these metabolites are derived from the main structure of PRZ, their presence proved PRZ degradation. Draft genome sequencing analysis of STP3 was performed to identify potential enzymes for PRZ biodegradation based on the metabolites found.
Previous studies on screening of lignin-degrading bacteria mainly focused on the ligninolytic ability of the isolated bacteria for the utilization of lignin monomers. In this study, we focused on the depolymerization of alkali lignin to prove the ability of the isolated thermophilic bacterial strains to utilize and depolymerize more than a monomer of alkali lignin within 7 days of incubation. Indigenous thermophilic bacterial isolates from the palm oil plantation were used to evaluate the depolymerization and utilization of alkali lignin. The confirmation of the bacterium-mediated depolymerization of oil palm empty fruit bunch was achieved through the removal of silica bodies, as observed with scanning electron microscopy. Stenotrophomonas sp. S2 and Bacillus subtilis S11Y were able to reduce approximately 50% and 20% of alkali lignin at 7 days of incubation without the requirement for additional carbon sources.
The natural coagulant Moringa oleifera lectin (MoL) as cationic protein is a promising candidate in coagulation process of water treatment plant. Introducing the gene encoding MoL into a host, Pichia pastoris, to secrete soluble recombinant protein is assessed in this study. Initial screening using PCR confirmed the insertion of MoL gene, and SDS-PAGE analysis detected the MoL protein at 8 kDa. Cultured optimization showed the highest MoL protein at 520 mg/L was observed at 28 °C for 144 h of culturing by induction in 1% methanol. Approximately, 0.40 mg/mL of recombinant MoL protein showed 95 ± 2% turbidity removal of 1% kaolin suspension. In 0.1% kaolin suspension, the concentration of MoL at 10 μg/mL exhibits the highest turbidity reduction at 68 ± 1%. Thus, recombinant MoL protein from P. pastoris is an effective coagulant for water treatment.
Tannery sludge, heavy metals (HMs) enriched hazardous solid waste, is produced extensively in many regions of the world. Even though the sludge is hazardous, it can be considered a material resource, if organic matter and HMs in the sludge can be stabilized to minimize its negative environmental impacts. This research aimed to evaluate the efficacy of using subcritical water (SCW) treatment for tannery sludge treatment through immobilization and thus reduction of HMs to mitigate their potential environmental risk and toxicity. HMs in the tannery sludge were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) and the average concentration of HMs (mg kg-1) was found in the following decreasing order of Cr (12 950) > Fe (1265) > Cu (76) > Mn (44) > Zn (36) > Pb (14) with very high Cr concentration. The result of toxicity characteristics leaching procedure and sequential extraction procedure tests revealed that the raw tannery sludge leachate contained 11.24 mg L-1 Cr, which classified the raw tannery sludge into a very high-risk category. After SCW treatment, the concentration of Cr in leachate was reduced to 1.6 mg L-1 indicating risk reduction to a low-risk category. The eco-toxicity levels of other HMs also decreased considerably after SCW treatment. X-ray diffractometry (XRD) and scanning electron microscopy (SEM) analysis were employed to identify the effective immobilizing substances formed in the SCW treatment process. The favorable formation of immobilizing orthorhombic tobermorite (Ca5Si6O16(OH)2·4H2O) at 240 °C in the SCW treatment process was confirmed by XRD and SEM analysis. The results confirmed that the formation of 11 Å tobermorite is capable of strongly immobilizing HMs in the SCW treatment process. Further, both orthorhombic 11 Å tobermorite and 9 Å tobermorite were successfully synthesized by SCW treatment on a mixture of tannery sludge including rice husk silica and Ca(OH)2 with water under rather mild conditions. Hence, it can be concluded that SCW treatment of tannery sludge with supplementary silica from rice husk can effectively immobilize the HMs and significantly reduce their environmental risk through tobermorite formation.
We report the complete genome sequence of Bacillus sp. strain PR5, isolated from a river receiving hospital and urban wastewater in Malaysia, which demonstrated a high capability for degrading prazosin. This genome sequence of 4,525,264 bp exhibited 41.5% GC content, 4,402 coding sequences, and 32 RNAs.
Musty odor production by actinomycetes is usually related to the presence of geosmin and 2-methylisoborneol (2-MIB), which are synthesized by enzymes encoded by the geoA and tpc genes, respectively. Streptomyces spp. strain S10, which was isolated from a water reservoir in Malaysia, has the ability to produce geosmin when cultivated in a basal salt (BS) solid medium, but no 2-MIB production occurred during growth in BS medium. Strain S10 could produce higher levels of geosmin when the phosphate concentration was limited to 0.05 mg/L, with a yield of 17.53 ± 3.12 ✕ 105 ng/L, compared with growth in BS medium. Interestingly, 2-MIB production was suddenly detected when the nitrate concentration was limited to 1.0 mg/L, with a yield of 1.4 ± 0.11 ✕ 105 ng/L. Therefore, it was concluded that phosphate- and nitrate-limiting conditions could induce the initial production of geosmin and 2-MIB by strain S10. Furthermore, a positive amplicon of geoA was detected in strain S10, but no tpc amplicon was detected by PCR analysis. Draft genome sequence analysis showed that one open reading frame (ORF) contained a conserved motif of geosmin synthase with 95% identity with geoA in Streptomyces coelicolor A3 (2). In the case of the tpc genes, it was found that one ORF showed 23% identity to the known tpc gene in S. coelicolor A3(2), but strain S10 lacked one motif in the N-terminus.