MyMedR

Displaying all 4 publications

Abstract:

Sort:

Catalyst free silica templated porous carbon nanoparticles from bio-waste materials

Kumar A, Hegde G, Manaf SA, Ngaini Z, Sharma KV

Chem Commun (Camb), 2014 Oct 28;50(84):12702-5.
PMID: 25100105 DOI: 10.1039/c4cc04378b

Porous Carbon Nanoparticles (PCNs) with well-developed microporosity were obtained from bio-waste oil palm leaves (OPL) using single step pyrolysis in nitrogen atmosphere at 500-600 °C in tube-furnace without any catalysis support. The key approach was using silica (SiO2) bodies of OPL as a template in the synthesis of microporous carbon nanoparticles with very small particle sizes of 35-85 nm and pore sizes between 1.9-2 nm.
Functionalized Carbon Nano-scale Drug Delivery Systems From Biowaste Sago Bark For Cancer Cell Imaging

Abdul Manaf SA, Hegde G, Mandal UK, Wui TW, Roy P

Curr Drug Deliv, 2017;14(8):1071-1077.
PMID: 27745545 DOI: 10.2174/1567201813666161017130612

BACKGROUND: Nano-scale carbon systems are emerging alternatives in drug delivery and bioimaging applications of which they gradually replace the quantum dots characterized by toxic heavy metal content in the latter application.
OBJECTIVE: The work intended to use carbon nanospheres synthesized from biowaste Sago bark for cancer cell imaging applications.
METHODS: This study synthesised carbon nanospheres from biowaste Sago bark using a catalyst-free pyrolysis technique. The nanospheres were functionalized with fluorescent dye coumarin-6 for cell imaging. Fluorescent nanosytems were characterized by field emission scanning electron microscopy-energy dispersive X ray, photon correlation spectroscopy and fourier transform infrared spectroscopy techniques.
RESULTS: The average size of carbon nanospheres ranged between 30 and 40 nm with zeta potential of -26.8 ± 1.87 mV. The percentage viability of cancer cells on exposure to nanospheres varied from 91- 89 % for N2a cells and 90-85 % for A-375 cells respectively. Speedy uptake of the fluorescent nanospheres in both N2a and A-375 cells was observed within two hours of exposure.
CONCLUSION: Novel fluorescent carbon nanosystem design following waste-to-wealth approach exhibited promising potential in cancer cell imaging applications.
Carbon nanomaterial properties help to enhance xylanase production from recombinant Kluyveromyces lactis through a cell immobilization method

Abdul Manaf SA, Mohamad Fuzi SFZ, Low KO, Hegde G, Abdul Manas NH, Md Illias R, et al.

Appl Microbiol Biotechnol, 2021 Nov;105(21-22):8531-8544.
PMID: 34611725 DOI: 10.1007/s00253-021-11616-0

Carbon nanomaterials, due to their catalytic activity and high surface area, have potential as cell immobilization supports to increase the production of xylanase. Recombinant Kluyveromyces lactis used for xylanase production was integrated into a polymeric gel network with carbon nanomaterials. Carbon nanomaterials were pretreated before cell immobilization with hydrochloric acid (HCl) treatment and glutaraldehyde (GA) crosslinking, which contributes to cell immobilization performance. Carbon nanotubes (CNTs) and graphene oxide (GO) were further screened using a Plackett-Burman experimental design. Cell loading and agar concentration were the most important factors in xylanase production with low cell leakage. Under optimized conditions, xylanase production was increased by more than 400% compared to free cells. Immobilized cell material containing such high cell densities may exhibit new and unexplored beneficial properties because the cells comprise a large fraction of the component. The use of carbon nanomaterials as a cell immobilization support along with the entrapment method successfully enhances the production of xylanase, providing a new route to improved bioprocessing, particularly for the production of enzymes. KEY POINTS: • Carbon nanomaterials (CNTs, GO) have potential as cell immobilization supports. • Entrapment in a polymeric gel network provides space for xylanase production. • Plackett-Burman design screen for the most important factor for cell immobilization.
Emergence of nanomaterials as potential immobilization supports for whole cell biocatalysts and cell toxicity effects

Abdul Manaf SA, Mohamad Fuzi SFZ, Abdul Manas NH, Md Illias R, Low KO, Hegde G, et al.

Biotechnol Appl Biochem, 2021 Dec;68(6):1128-1138.
PMID: 32969042 DOI: 10.1002/bab.2034

The traditional approach of fermentation by a free cell system has limitations of low productivity and product separation that need to be addressed for production enhancement and cost effectiveness. One of potential methods to solve the problems is cell immobilization. Microbial cell immobilization allows more efficient up-scaling by reducing the nonproductive growth phase, improving product yield and simplifying product separation. Furthermore, the emergence of nanomaterials such as carbon nanotubes, graphene, and metal-based nanomaterials with excellent functional properties provides novel supports for cell immobilization. Nanomaterials have catalytic properties that can provide specific binding site with targeted cells. However, the toxicity of nanomaterials towards cells has hampered its application as it affects the biological system of the cells, which cannot be neglected in any way. This gray area in immobilization is an important concern that needs to be addressed and understood by researchers. This review paper discusses an overview of nanomaterials used for cell immobilization with special focus on its toxicological challenges and how by understanding physicochemical properties of nanomaterials could influence the toxicity and biocompatibility of the cells.