Displaying all 11 publications

Abstract:
Sort:
  1. Nur Aini Abu Bakar, Malina Osman, Huzlinda Hussin
    MyJurnal
    Introduction: Endometrial carcinoma is the most common gynaecological malignancy in developed countries and the sixth most common cancer among women worldwide. Cancer staging is vital in treatment decisions and the prediction of prognoses, and is based on imaging studies, histological results and surgery. Therefore, a simple and fast preoperative tool to predict the precise cancer stage of patients is needed. CA 125, a cancer antigen, is used in assessing therapeutic response and cancer surveillance in endometrial carcinoma. However, this tumour marker is not routinely performed in the mentioned circumstances. Studies have shown that preoperative CA 125 was significantly high in patients in a higher stage of endometrial cancer. Thus, this study aims to assess the primary role of CA 125 in predicting the stage of endometrial carcinoma, by correlating preoperative serum CA 125 with clinicopathological parameters. Method: The retrospective data of endometrial carcinoma cases consisting of de- mographic and clinicopathological parameters as well as preoperative serum CA 125 levels were retrieved from Laboratories Information System (LIS) at Hospital Selayang, Selangor, Malaysia, from January 2000 until June 2016. Only 20 cases fulfilled the inclusion and exclusion criteria. Preoperative serum CA 125 was correlated with demographic and clinicopathological parameters, and was analysed using a Kruskal-Wallis test. Results: There was a significant association between elevated serum CA 125 with myometrial and cervical stroma invasion in endometrial carcinoma (p
  2. Nur Aliaa, Eusni Rahayu Mohd Tohit, Nik Hafidzah Nik Mustapha, Malina Osman
    MyJurnal
    Introduction: Increased monocyte percentage and monocyte anisocytosis were suggested as new markers for den- gue fever detection. This study aims to investigate and evaluate monocyte volume standard deviation (MoV-SD) and monocyte percentage (Mono %) parameters using Coulter automated haematology analyser as screening parameters in discriminating between dengue infection and other febrile illness. Methods: A cross-sectional laboratory analysis using suspected dengue fever patients were included in this study. The study was conducted in the Department of Pathology, Hospital Tuanku Jaafar Seremban from June 2016 until June 2017. Patients were classified into dengue positive and dengue negative based on dengue IgM and NS1 result. The diagnostic performance of MoV-SD and Mono % was analysed by receiver operating characteristic (ROC) curve analysis. The cut-off value of the MoV-SD and Mono % was determined and evaluated with the validation group. Chi-square test was used to assess the as- sociation between the parameters. Results: 88 (48.4%) from 182 samples were confirmed to have dengue infection. ROC curve analysis showed Mono % at cut off value of 10.5 % with area under the curve (AUC) of 0.869 with 84.1% sensitivity and 84% specificity (95% CI: 0.812-0.925) and MoV-SD cut off value at 22.2 (AUC 0.776, 80.7% sensitivity, 61.7% specificity, 95% CI: 0.709-0.843) are an excellent parameters in separating dengue positive and dengue-negative patients. A cut-off value of 10.5 of Mono % and 22.2 of MoV-SD were applied to the validation group showed 83.1%, 66.4% sensitivity and 84.9%, 77.3% specificity respectively. Conclusion: MoV-SD and Mono
    % parameters are a potential parameter for the screening of dengue infection in acute febrile illness patients with good specificity and sensitivity.
  3. Fatma Ibrahem Alaribi, Ngah Zasmy Unyah, Norashiqin Misni, Siti Norbaya Masri, Malina Osman
    MyJurnal
    Introduction: Soil-transmitted helminths (STHs) are considered one of the neglected parasitic diseases that cause ma- jor health problems and other related complications. The aim of this study was to determine the prevalence of STHs and anaemia among school children of little-known refugee communities in Malaysia. Methods: A cross-sectional study was conducted among 148 refugee school children in the Klang Valley, Malaysia, from 2017 to 2019. Data were collected using a questionnaire, in addition to haemoglobin testing while faecal samples were screened for STHs eggs using the formalin-ether concentration technique. Results: The result showed that the prevalence of STHs in this study was 37.2%. Trichuris trichiura infection was the most prevalent (46.4%) followed by Ascaris lumbri- coides (44.9%) and hookworms (8.7%). About 37.8 % of the respondents were found to be anaemic. Binary logistic regression showed significant infection with STHs complicated with anaemia (AOR: 3.67, 95 % CI 1.595-8.5). Con- clusion: The evidence from this study suggests proper and strategic interventions to reduce morbidity. An alternative and efficient system is needed to provide the schoolchildren with basic care for sanitation and health provision. It is recommended that voluntary clinics provided by non-government organisations focus on counselling and advice on personal hygiene of the children.
  4. Nurimatussolehah Sarijan, Sabariah Md Noor, Tun Maizura Mohd Fathullah, Malina Osman, Zainina Seman
    MyJurnal
    Percentage of haemolysis is widely used as a quality parameter to assess red blood cell viability in blood banking. In certain blood banks, serum potassium level is used due to the unavailability of the former test. The relationship between these two tests, however, is still unclear. The objective of this study is to determine the association between haemolysis measured using two different methods for quality control. Methods: A total of forty-four samples of packed red cell in citrate-phosphate-dextrose with optisol were randomly selected from donation drives. Nine millilitres of blood was collected weekly starting from day-2 of storage, followed by day-7, 14, 21, 28, 35 and 42 for assessment of red blood cell haemolysis by measuring serum potassium level and percentage of haemolysis.Results: These two parameters were correlated significantly with a positive moderate linear relationship on day 7, 21 and 28 with r = 0.393, 0.448 and 0.425, respectively and p-values less than 0.01. The linear regression analysis showed there was a significant regression equation which could be used to predict the serum potassium level from the percentage of haemolysis. Conclusion: There were significant increases in the percentage of haemolysis and serum potassium level in the packed red cell units with storage. The serum potassium level would be able to be predicted from the percentage of haemolysis using the regression equations on day 7, 21 and 28. The serum potassium measurement could be used as an alternative test to the percentage of haemolysis before issuing blood.
  5. Mohd Rohaizad Md Roduan, Norhafizah Mohtarrudin, Chong PP, Malina Osman, Noraini Mohd Dusa
    Sains Malaysiana, 2015;44:727-733.
    Inflammation plays an important role to the process of prostate carcinogenesis by increasing the rate of cell proliferation,
    which contributes to an aggressive tumour phenotype. Cyclooxygenase-2 (COX-2) has been found overexpressed in
    various types of cancer cells including prostate. The aim of this study was to investigate the COX-2 expressions in different
    types of human prostate tissues. Paraffin-embedded prostate tissues from 263 samples were examined for the expression
    of COX-2 marker by immunohistochemistry method. COX-2 was found highly expressed in prostate adenocarcinoma
    (p=0.001) as compared to benign and normal tissues. The score of COX-2 expressions in most of normal prostate was
    weak 49 (77.8%), while only 16 (16%) of BPH showed strong expression. 56 cases (56%) prostate cancer showed strong
    COX-2 expression. Prostate cancer cases showed significant differences in staining patterns as tumour grade increased.
    In addition, COX-2 expression was significantly correlated with Gleason score in cancerous tissues. This study suggests
    that COX-2 overexpression is associated with prostate cancer and higher grade tumour.
  6. Jannaltul Adni Azmi, Subashini C. Thambiah, Hanisah Abdul Hamid, Siti Yazmin Zahari Sham, Intan Nureslyna Samsudin, Malina Osman
    MyJurnal
    Introduction: Total calcium concentration is widely used to assess body calcium status although limited by many
    confounding factors. Thus, this study aimed to derive and internally validate an albumin-adjusted calcium equation
    for a selected Malaysian population. Method: This cross-sectional study involved 1011 adults at an emergency
    department of a tertiary hospital. Patients who had total calcium, ionised calcium and albumin measurements
    taken simultaneously were included. Derivation of the albumin-adjusted calcium equation was based on the
    adjustment equation obtained from the Association for Clinical Biochemistry and Laboratory Medicine 2015
    position paper. Additionally, the equation was internally validated and compared with ionised calcium
    (gold standard) and the conventional Payne’s equation. Results: The newly derived equation =
    total calcium + 0.017 (41.35 – albumin). Internal validation exhibited the amount of shrinkage of 0.049.
    It tends to overestimate the adjusted calcium by a mean difference of 0.029 mmol/L compared to Payne’s equation.
    The comparison between Payne’s equation and the new equation with ionised calcium reclassified
    402 and 486 patients, respectively into different calcium status. When both equations were compared,
    calcium status classification significantly differed in all and hypoalbuminaemic subjects by 90 and 16 patients,
    respectively. Conclusion: Locally derived albumin-adjusted calcium equation differed statistically in calcium
    status classification when compared to the Payne’s equation. However, to confirm this significance, the
    result must be compared to ionised calcium under strict, controlled preanalytical conditions. In terms of clinical
    significance, there was no difference in classification of calcium status between Payne’s and the new equation at
    medical decision limits
  7. Thambiah, Subashini Chellapah, Zalinah Ahmad, Zarida Hambali, Malina Osman, Munia Mohd Zain, Fuziah Md Zain, et al.
    MyJurnal
    A clinical descriptive study was done to determine the sociodemographic, laboratory and clinical characteristics of patients with congenital adrenal hyperplasia (CAH) referred to Hospital Putrajaya, a tertiary endocrine centre in Malaysia. Electronic laboratory data of 51 CAH patients were obtained. The demographics and clinical details of the study population were acquired from a questionnaire completed by parents of participants. There were 25 males (49%) and 26 females (51%), of which, 58.8% were Malays. Median age of participants was 4 years whilst median age at diagnosis of CAH was two years. Parental consanguinity was documented in three patients (5.9%). Patients originated from Johor (19.6%), Selangor (19.6%), Negeri Sembilan (17.6%) and Kedah (13.7%). Majority of patients were diagnosed after one week of life (80.4%) although more females were diagnosed under the age of one week compared to males (p=0.041). Most females presented with ambiguous genitalia (42.3%) [p=0.001] whereas 72% of males presented with salt wasting (p=0.003). No significant associations between race and all other variables, though interestingly three Malay patients presented with ambiguous genitalia and hypertension. Equal gender distribution noted as expected in an autosomal recessive condition, although not in keeping with other Asian countries. Early diagnosis in females attributed to obvious genital ambiguity at birth. Varied clinical presentation, although in minority, necessitates genetic studies for prompt diagnosis and treatment. Considering that majority of patients presented with salt wasting and the age at diagnosis was delayed, the introduction of a neonatal screening programme is essential in Malaysia.
  8. Norhafizah Mohtarrudin, Mhd Shidee Mhd Nasir, Rosila Abu Bakar, Malina Osman, Koey, Peng Mun, Suryati Mohd Yusoff
    MyJurnal
    Renal cell carcinoma (RCC) is the most common renal malignancies. In advanced stage, it is highly resistant to systemic therapies. RCC is a highly vascular tumour and angiogenesis pathway has been postulated in its carcinogenesis. Novel drug targeting Vascular Endothelial Growth Factor (VEGF) and advanced surgical interventions have shown to increase the overall patients’ survival. In this study, we evaluated the VEGF expression of RCC using immunohistochemistry technique and its potential correlation with the tumour grades. Methods: 40 RCC cases that underwent nephrectomy were selected. The archived samples of formalin fixed, paraffin-embedded (FFPE) were retrieved. The tumour tissue blocks were carefully chosen, sectioned and stained with VEGF using immunohistochemistry technique. The intensity of VEGF expression was scored as 0 (negative), 1+ (weak), 2+ (moderate) or 3+ (strong). Results: Majority of the RCC cases were male, with male to female ratio of 2.1:1. Mean patient age was 56.2 years (age ranged between 16 to 74 years). Most of the cases were Malays (42.5 %). VEGF was expressed in 36 (90%) of RCC cases. Among the 36 cases that were immunopositive, 8 (16.7%) were grade 1, 20 (55.6%) grade 2 and 8 (16.7%) grade 3. There was no significant association between VEGF expressions score and grades of RCC (p=0.39). Conclusion: VEGF was expressed in majority of RCC cases although there was no significant association with tumour grades.
  9. Umar S, Shinkafi SH, Hudu SA, Neela V, Suresh K, Nordin SA, et al.
    Ann Parasitol, 2017;63(2):133-139.
    PMID: 28822206 DOI: 10.17420/ap6302.97
    Schistosomiasis is the major source of morbidity in Sub-Saharan Africa and Asia. It is estimated that 207 million people are infected, of which 97% are in Africa. The aim of this study was the determining of prevalence as well as the phylogeny of S. haematobium among school children in Argungu Emirate, Kebbi State Nigeria. A total of 325 urine samples was collected from school children between 7 to 14 years. S. heamatobium eggs was examined under dissecting microscope and DNA was extracted from urine sample and COX1 gene was amplified by nested PCR. The PCR products were purified, sequenced and analysed. This study showed a prevalence of 32.09%, with male pupils having the highest prevalence. S. haematobium infections in children who fetch water in the river have 24 times higher risk of being infected while those who bath in the river have 158 times higher risk of being infected. Our sequences were phylogenetically related to S. haematobium isolate U82266 from Kenya and consistence with the predominant species in Africa. This was the first S. haematobium and S. mansoni co-infection reported in Nigeria. S. haematobium infection is prevalent among school age and significantly associated with water contact.
  10. Saizan S, Sukeri S, Zahiruddin WM, Shafei MN, Rukman AH, Malina O, et al.
    Int J Health Plann Manage, 2019 Jan;34(1):e817-e823.
    PMID: 30357908 DOI: 10.1002/hpm.2696
    Leptospirosis is an endemic disease in Malaysia. Despite the increasing incidence rate, knowledge on the economic assessment of preventing leptospirosis is still limited. This paper introduces the willingness-to-contribute (WTC) method for estimating the economic benefit of preventing leptospirosis. A cross-sectional study using the WTC method was applied to measure how much time respondents in Kelantan were willing to contribute toward preventing leptospirosis. Study respondents were wet market traders aged 18 years old and above who were fluent in the Malay language. The average WTC value was multiplied by the population of Kelantan to derive the monetary value of preventing leptospirosis. Two hundred and fifty respondents participated in the study. The mean time contribution was 6.68 hours (SD9.01) per month. The average WTC corresponded to a monthly cost savings of US$4.94 per person. Approximately between US$106.7 million to US$315 million per annum can be saved through the prevention of leptospirosis in Kelantan. Preventing leptospirosis is beneficial to Kelantan and would bring major economic savings. The findings are intended to help policy makers in the planning and management of leptospirosis policies and interventions.
  11. Zahiruddin WM, Arifin WN, Mohd-Nazri S, Sukeri S, Zawaha I, Bakar RA, et al.
    BMC Public Health, 2018 03 07;18(1):331.
    PMID: 29514645 DOI: 10.1186/s12889-018-5234-y
    BACKGROUND: In Malaysia, leptospirosis is considered an endemic disease, with sporadic outbreaks following rainy or flood seasons. The objective of this study was to develop and validate a new knowledge, attitude, belief and practice (KABP) questionnaire on leptospirosis for use in urban and rural populations in Malaysia.

    METHODS: The questionnaire comprised development and validation stages. The development phase encompassed a literature review, expert panel review, focus-group testing, and evaluation. The validation phase consisted of exploratory and confirmatory parts to verify the psychometric properties of the questionnaire. A total of 214 and 759 participants were recruited from two Malaysian states, Kelantan and Selangor respectively, for the validation phase. The participants comprised urban and rural communities with a high reported incidence of leptospirosis. The knowledge section of the validation phase utilized item response theory (IRT) analysis. The attitude and belief sections utilized exploratory factor analysis (EFA) and confirmatory factor analysis (CFA).

    RESULTS: The development phase resulted in a questionnaire that included four main sections: knowledge, attitude, belief, and practice. In the exploratory phase, as shown by the IRT analysis of knowledge about leptospirosis, the difficulty and discrimination values of the items were acceptable, with the exception of two items. Based on the EFA, the psychometric properties of the attitude, belief, and practice sections were poor. Thus, these sections were revised, and no further factor analysis of the practice section was conducted. In the confirmatory stage, the difficulty and discrimination values of the items in the knowledge section remained within the acceptable range. The CFA of the attitude section resulted in a good-fitting two-factor model. The CFA of the belief section retained low number of items, although the analysis resulted in a good fit in the final three-factor model.

    CONCLUSIONS: Based on the IRT analysis and factor analytic evidence, the knowledge and attitude sections of the KABP questionnaire on leptospirosis were psychometrically valid. However, the psychometric properties of the belief section were unsatisfactory, despite being revised after the initial validation study. Further development of this section is warranted in future studies.

Related Terms
Filters
Contact Us

Please provide feedback to Administrator ([email protected])

External Links