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  1. Abdul, R., Hasnain, J., Zarfishan, T., Aina, S. Z., Warda, F., Iftkhar, A., et al.
    MyJurnal
    This study was aimed to find out the prevalence of HCV infection and frequency of its genotypes in the
    population of the Karol war village i.e. situated in Lahore, Punjab, Pakistan. It was a descriptive crosssectional study model. Tests were performed in the Department of Bacteriology, Institute of Public
    Health Lahore, Pakistan. Cases positive for Anti-HCV antibodies by chemiluminescence assay were
    subjected to HCV RNA detection by PCR. Genotyping was done by Geno-Sen’s HCV Genotyping
    1/2/3/4 Real Time PCR kit for Rotor Gene. A total of 396 patients were enrolled for this study. The
    incidence of positive HBSAg and HCV cases were 11.86% and 1.5% respectively. Males were more
    affected with 61.7% (n=29) while positive cases in females were 38.3% (n=18). Age group of 31-45
    years had higher infection rate among other ages (n=20). The frequency of genotypes 3, 1 and 2 was
    36, 4 and 3 respectively while the genotype of 4 patients was found to be indeterminate. Genotype 3 is
    the most prevalent genotype in the studied individuals which corresponds to the local data available so
    far however 11.86 % positivity figure is quite alarming figure for that area.
  2. Iftkhar A, Hasan IJ, Sarfraz M, Jafri L, Ashraf MA
    West Indian Med J, 2015 12;64(5):462-467.
    PMID: 27398602 DOI: 10.7727/wimj.2016.052
    Background: The present study was designed to evaluate the nephroprotective effect of the leaves of Aloe barbadensis against toxicity induced by diclofenac sodium in albino rabbits.

    Subjects and Method: Thirty-six healthy albino rabbits were randomly divided into six groups each with six animals. Group 1 served as the untreated control, group 2 was treated only with diclofenac sodium, group 3 with the nephroprotective drug silymarin and groups 4, 5, and 6 were treated with different doses of Aloe barbadensis,ie 200 mg/kg, 400 mg/kg and 600 mg/kg, respectively after being treated with diclofenac sodium. Blood samples were collected after every five days up to fifteen days. Haematological and histopathological parameters were determined by using diagnostic kits.

    Results: Results of haematological studies showed that use of the powder of Aloe barbadensis normalized the level of different factors eg, white blood cells (WBCs), red blood cells (RBCs), platelet count, packed cell volume (PCV), mean cell volume (MCV) and haemoglobin (Hb) values. Histopathological studies showed that Aloe barbadensis ameliorated pyknotic nuclei in the renal epithelial cells and reduced oxidative stress by increasing the level of catalase and decreasing malondialdehyde (MDA) level.

    Conclusion: These results have shown that Aloe barbadensis can normalize oxidative stress and can be used as an effective nephroprotective agent against drug-induced nephrotoxicity.

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