MyMedR

Displaying all 4 publications

Abstract:

Sort:

Case Report: Management of a Rare Case of Neonatal Uterovaginal Prolapse Using a Vaginal Plug

Gunasagran Y, Wahab AVA

J Pediatr Adolesc Gynecol, 2021 Sep 10.
PMID: 34517116 DOI: 10.1016/j.jpag.2021.09.001

Congenital neonatal uterovaginal prolapse is a rare occurrence that is usually detected after delivery. Most cases are managed conservatively, although there are more invasive approaches to management. We report a case of a neonate with uterovaginal prolapse that we successfully managed conservatively. In this case, a vaginal plug made from a wet cotton ball and condom was inserted into the vaginal canal. To further reinforce the vaginal plug, a pressure garment was applied over the neonate's diaper. A trial of noninvasive methods that are simple and cost-effective should always precede other, more invasive methods in managing cases such as this.
Corrigendum to "Aberrant upregulation of CDK1 contributes to medroxyprogesterone acetate (MPA) resistance in cancer-associated fibroblasts of the endometrium" [Biochem. Biophys. Res. Commun. 628 (2022) 133-140]

Omar IS, Mat Adenan NA, Godoy A, Teo IH, Gunasagran Y, Chung I

Biochem Biophys Res Commun, 2023 Jan 15;640:202.
PMID: 36593136 DOI: 10.1016/j.bbrc.2022.12.065
Oral rehydration therapy versus intravenous rehydration therapy in the first 12 h following hospitalization for hyperemesis gravidarum: A randomized controlled trial

Wan Jabarudin WNE, Narayanan V, Hamdan M, Gunasagran Y, Thavarajan RD, Kamarudin M, et al.

Int J Gynaecol Obstet, 2024 Feb 15.
PMID: 38358264 DOI: 10.1002/ijgo.15429

OBJECTIVE: To evaluate oral rehydration therapy (ORT) compared with intravenous rehydration therapy (IVT) in the early inpatient management of hyperemesis gravidarum (HG).
METHODS: A total of 124 women hospitalized for HG from February 10, 2021 till January 6, 2023 were randomized to ORT (n = 61) or IVT (n = 63) for an initial 12 h. Inclusion criteria includes women older than 18 years, with a viable intrauterine pregnancy less than 14 weeks at their first hospitalization for HG with ketonuria of at least 2+. Primary outcomes were (1) satisfaction score with allocated intervention, (2) weight change, and (3) ketonuria change at 12 h. Secondary outcomes included vomiting frequency, nausea score, serial vital signs, hematocrit and electrolyte levels at 12 h, deviation from treatment protocol (cross-over therapy), participant recommendation of allocated treatment to a friend, and length of hospital stay.
RESULTS: Primary outcomes of (1) participant satisfaction score (on a 0-10 visual numerical rating scale) was 7 (interquartile range [IQR] 5-8) versus 9 (IQR 8-10), P
Distinct Lipid Phenotype of Cancer-Associated Fibroblasts (CAFs) Isolated From Overweight/Obese Endometrial Cancer Patients as Assessed Using Raman Spectroscopy

Yeu TH, Omar IS, Sani SFA, Pathmanathan D, Goh BT, Ravindran N, et al.

Appl Spectrosc, 2023 Jul;77(7):723-733.
PMID: 37357678 DOI: 10.1177/00037028231182721

Obesity is strongly linked with increased risk and poorer prognosis of endometrial cancer (EC). Cancer-associated fibroblasts (CAFs) are activated fibroblasts that form a large component of the tumor microenvironment and undergo metabolic reprogramming to provide critical metabolites for tumor growth. However, it is still unknown how obesity, characterized by a surplus of free fatty acids drives the modifications of CAFs lipid metabolism which may provide the mechanistic link between obesity and EC progression. The present study aims to evaluate the utility of Raman spectroscopy, an emerging nondestructive analytical tool to detect signature changes in lipid metabolites of CAFs from EC patients with varying body mass index. We established primary cultures of fibroblasts from human EC tissues, and CAFs of overweight/obese and nonobese women using antibody-conjugated magnetic beads isolation. These homogeneous fibroblast cultures expressed fibroblast markers, including α-smooth muscle actin and vimentin. Analysis was made in the Raman spectra region best associated with cancer progression biochemical changes in lipids (600-1800 cm-1 and 2800-3200 cm-1). Direct band analysis and ratiometric analysis were conducted to extract information from the Raman spectrum. Present results demonstrated minor shifts in the CH2 symmetric stretch of lipids at 2879 cm-1 and CH3 asymmetric stretching from protein at 2932 cm-1 in the overweight/obese CAFS compared to nonobese CAFs, indicating increased lipid content and a higher degree of lipid saturation. Principal component analysis showed that CAFs from overweight/obese and nonobese EC patients can be clearly distinguished indicating the capability of Raman spectroscopy to detect changes in biochemical components. Our results suggest Raman spectroscopy supported by chemometric analysis is a reliable technique for characterizing metabolic changes in clinical samples, providing an insight into obesity-driven alteration in CAFs, a critical stromal component during EC tumorigenesis.