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Toward understanding the distribution of Laurasian frogs: a test of Savage's biogeographical hypothesis using the genus Bombina

Zheng Y, Fu J, Li S

Mol Phylogenet Evol, 2009 Jul;52(1):70-83.
PMID: 19348953 DOI: 10.1016/j.ympev.2009.03.026

Several anuran groups of Laurasian origin are each co-distributed in four isolated regions of the Northern Hemisphere: central/southern Europe and adjacent areas, Korean Peninsula and adjacent areas, Indo-Malaya, and southern North America. Similar distribution patterns have been observed in diverse animal and plant groups. Savage [Savage, J.M., 1973. The geographic distribution of frogs: patterns and predictions. In: Vial, J.L. (Ed.), Evolutionary Biology of the Anurans. University of Missouri Press, Columbia, pp. 351-445] hypothesized that the Miocene global cooling and increasing aridities in interiors of Eurasia and North America caused a southward displacement and range contraction of Laurasian frogs (and other groups). We use the frog genus Bombina to test Savage's biogeographical hypothesis. A phylogeny of Bombina is reconstructed based on three mitochondrial and two nuclear gene fragments. The genus is divided into three major clades: an Indo-Malaya clade includes B. fortinuptialis, B. lichuanensis, B. maxima, and B. microdeladigitora; a European clade includes B. bombina, B. pachypus, and B. variegata; and a Korean clade contains B. orientalis. The European and Korean clades form sister-group relationship. Molecular dating of the phylogenetic tree using the penalized likelihood and Bayesian analyses suggests that the divergence between the Indo-Malaya clade and other Bombina species occurred 5.9-28.6 million years ago. The split time between the European clade and the Korean clade is estimated at 5.1-20.9 million years ago. The divergence times of these clades are not significantly later than the timing of Miocene cooling and drying, and therefore can not reject Savage's hypothesis. Some other aspects of biogeography of Bombina also are discussed. The Korean Peninsula and the Shandong Peninsula might have supplied distinct southern refugia for B. orientalis during the Pleistocene glacial maxima. In the Indo-Malaya clade, the uplift of the Tibetan Plateau might have promoted the split between B. maxima and the other species.
Carboxymethyl cellulose/sodium alginate beads incorporated with calcium carbonate nanoparticles and bentonite for phosphate recovery

Fu J, Yap JX, Leo CP, Chang CK

Int J Biol Macromol, 2023 Apr 15;234:123642.
PMID: 36791941 DOI: 10.1016/j.ijbiomac.2023.123642

Although anionic polyelectrolyte hydrogel beads offer attractive adsorption of cationic dyes, phosphate adsorption is limited by electrostatic interactions. In this work, carboxymethyl cellulose (CMC)/sodium alginate (SA) hydrogel beads were modified with calcium carbonate (CaCO3) and/or bentonite (Be). The compatibility between CaCO3 and Be was proven by the homogeneous surface, as shown in the scanning electron microscopic images. Fourier-transform infrared and X-ray diffraction spectra further confirmed the existence of inorganic filler in the hydrogel beads. Although CMC/SA/Be/CaCO3 hydrogel beads attained the highest methylene blue and phosphate adsorption capacities (142.15 MB mg/g, 90.31 P mg/g), phosphate adsorption was significantly improved once CaCO3 nanoparticles were incorporated into CMC/SA/CaCO3 hydrogel beads. The kinetics of MB adsorption by CMC/SA hydrogel beads with or without inorganic fillers could be described by the pseudo-second-order model under chemical interactions. The phosphate adsorption by CMC/SA/Be/CaCO3 hydrogel beads could be explained by the Elovich model due to heterogeneous properties. The incorporation of Be and CaCO3 also improved the phosphate adsorption through chemical interaction since Langmuir isotherm fitted the phosphate adsorption by CMC/SA/Be/CaCO3 hydrogel beads. Unlike MB adsorption, the reusability of these hydrogel beads in phosphate adsorption reduced slightly after 5 cycles.
Probiotic and Safety Evaluation of Twelve Lactic Acid Bacteria as Future Probiotics

Hu Y, Xie Y, Su Q, Fu J, Chen J, Liu Y

Foodborne Pathog Dis, 2023 Nov;20(11):521-530.
PMID: 37722019 DOI: 10.1089/fpd.2023.0039

The human gut flora is highly diverse. Most lactic acid bacteria (LAB) are widely used as probiotics in human and animal husbandry and have a variety of physiological benefits. This article mainly studied the bacteriostatic ability of LAB against four pathogenic bacteria, gastrointestinal environment tolerance, and adhesion ability to Caco-2 cells. The genome of Lactiplantibacillus plantarum L461 was sequenced and analyzed. The results showed that strains F512, L461, and D469 had the most significant inhibitory effects on Escherichia coli, Salmonella enterica B, Staphylococcus aureus, and Listeria monocytogenes. In addition, strains L461, C502, and P231 showed good tolerance after exposure to simulated gastric fluid for 0-4 h. Strains C502, H781, and L461 showed good tolerance in simulated intestinal fluid. Strains L461 and H781 showed good adhesion to Caco-2 cells. The number of viable bacteria was more than 60. Therefore, we screened L. plantarum L461 from 12 LAB strains through three aspects of evaluation, and conducted whole genome sequencing and analysis. Sequencing results showed that L. plantarum L461 had more defense mechanisms and phage annotation genes than L. plantarum WCFS1. Virulence factor studies showed that L. plantarum L461 has iron absorption system and adhesion-related gene annotation, indicating that L. plantarum L461 has survival advantage in intestinal tract. The predicted results showed that there were eight phages with phage resistance in L. plantarum L461. L. plantarum L461 is sensitive to several antibiotics, notably penicillin and oxacillin. In summary, the results of this study prove that L. plantarum L461 has good prebiotic function and is safe. Therefore, L. plantarum L461 can be safely used as a potential functional probiotic.
Enhanced photocatalytic regeneration of carboxymethyl cellulose/lignin/ZnO complex hydrogel after methylene blue adsorption

Fu J, Yap JX, Leo CP, Chang CK

Int J Biol Macromol, 2024 Aug;274(Pt 2):133510.
PMID: 38960270 DOI: 10.1016/j.ijbiomac.2024.133510

The biodegradable, nontoxic, and renewable carboxymethyl cellulose (CMC) hydrogel has been developed into a green adsorbent. However, the weak chemical interaction limits its adsorption capability and reusability. This work incorporated lignin with complex structure and ZnO nanoparticles with photocatalytic properties into CMC hydrogel beads to improve the removal of methylene blue (MB) through chemical interaction. Scanning electron microscopic images and Fourier-transform infrared spectra confirmed the compatibility between lignin and ZnO nanoparticles as well as the increment of active sites for dye removal. The MB adsorption on CMC hydrogel beads was more significantly affected by the temperate and initial concentration compared to contact time, pH, and adsorbent dosage. The MB adsorption capacity of CMC hydrogel was improved to 276.79 mg/g after incorporating lignin and ZnO nanoparticles. The adsorption followed the pseudo-second-order kinetic model and Langmuir isotherm model, indicating chemical adsorption. After 6 cycles, the adsorption capacity was reduced by about 15 %. The UV irradiation could recover and improve MB adsorption capacity of CMC hydrogel beads containing ZnO nanoparticles due to the introduction of reactive oxygen species.
Fulltext Preparation and characterization of curdlan/polyvinyl alcohol/ thyme essential oil blending film and its application to chilled meat preservation

Zhang Y, Zhou L, Zhang C, Show PL, Du A, Fu J, et al.

Carbohydr Polym, 2020 Nov 01;247:116670.
PMID: 32829798 DOI: 10.1016/j.carbpol.2020.116670

With the growing interest in food safety and in environmental protection, it is more attractive to develop novel biodegradable packaging films. In this regard, one new blending film was prepared with curdlan (CD)/polyvinyl alcohol (PVA)/thyme essential oil. Our results demonstrated that the mechanical properties of the blending film were the best when the ratio of the CD and PVA was 4:1. Further, the barrier properties of the film were optimized by incorporating with thyme essential oil. It was proved that not only water vapor permeability was lower, but also the elongation at break was improved, when 2% (w/w) thyme essential oil used. The potential interactions of the film matrix were analyzed by FTIR, XRD and Cryo-scanning electron microscopy. Importantly, both the antioxidant activity and antibacterial activity were improved. Finally, the blending film was employed for the preservation of chilled meat, while the shelf life was extended up to 10 days.
Fulltext Population structure, demographic history and local adaptation of the grass carp

Shen Y, Wang L, Fu J, Xu X, Yue GH, Li J

BMC Genomics, 2019 Jun 07;20(1):467.
PMID: 31174480 DOI: 10.1186/s12864-019-5872-1

BACKGROUND: Genetic diversity within a species reflects population evolution, ecology, and ability to adapt. Genome-wide population surveys of both natural and introduced populations provide insights into genetic diversity, the evolutionary processes and the genetic basis underlying local adaptation. Grass carp is the most important freshwater foodfish species for food and water weed control. However, there is as yet no overall picture on genetic variations and population structure of this species, which is important for its aquaculture.
RESULTS: We used 43,310 SNPs to infer the population structure, evidence of local adaptation and sources of introduction. The overall genetic differentiation of this species was low. The native populations were differentiated into three genetic clusters, corresponding to the Yangtze, Pearl and Heilongjiang River Systems, respectively. The populations in Malaysia, India and Nepal were introduced from both the Yangtze and Pearl River Systems. Loci and genes involved in putative local selection for native locations were identified. Evidence of both positive and balancing selection was found in the introduced locations. Genes associated with loci under putative selection were involved in many biological functions. Outlier loci were grouped into clusters as genomic islands within some specific genomic regions, which likely agrees with the divergence hitchhiking scenario of divergence-with-gene-flow.
CONCLUSIONS: This study, for the first time, sheds novel insights on the population differentiation of the grass carp, genetics of its strong ability in adaption to diverse environments and sources of some introduced grass carp populations. Our data also suggests that the natural populations of the grass carp have been affected by the aquaculture besides neutral and adaptive forces.
Fulltext Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations

Jiang B, Fu J, Dong Z, Fang M, Zhu W, Wang L

PeerJ, 2019;7:e7007.
PMID: 31179190 DOI: 10.7717/peerj.7007

Background: Many tilapia species or varieties have been widely introduced and have become an economically important food fish in China. Information on the genetic backgrounds of these populations is deficient and requires more research, especially for red tilapia strains.
Methods: In the present study, displacement loop (D-loop) sequences were used to evaluate the genetic relationship and diversity of seven tilapia populations that are widely cultured in China; this was done specifically to speculate on the maternal ancestry of red tilapia strains. Three red tilapia varieties of Oreochromis ssp., Taiwan (TW), Israel (IL), and Malaysia (MY) strains and other populations, including O. aureus (AR), O. niloticus (NL), O. mossambicus (MS), and the GIFT strain of O. niloticus, were collected and analyzed in this study.
Results: A total of 146 polymorphic sites and 32 haplotypes of D-loop sequences were detected among 332 fish and four major haplotypes were shared among the populations. The TW and NL populations had a greater number of haplotypes (20 and 8, respectively). The haplotype diversity (Hd) and nucleotide diversity (π) of each population ranged from 0.234 to 0.826, and 0 to 0.060, respectively. The significant positive Tajima's D value of neutral test were detected in the NL, IL, and MY populations (P 0.05). The nearest K2P genetic distance (D = 0.014) was detected between the MS and TW populations, whereas, the farthest (D = 0.101) was found between the GIFT and AR populations. The results from the molecular variance analysis (AMOVA) showed that there was an extremely significant genetic variation observed among the populations (P
Enhancing electronic metal support interaction (EMSI) over Pt/TiO2 for efficient catalytic wet air oxidation of phenol in wastewater

Fu J, Zhang X, Li H, Chen B, Ye S, Zhang N, et al.

J Hazard Mater, 2022 Mar 15;426:128088.
PMID: 34959211 DOI: 10.1016/j.jhazmat.2021.128088

Phenol is one of the major hazardous organic compounds in industrial wastewater. In this work, a highly active Pt/TiO2 catalyst for catalytic wet air oxidation (CWAO) of phenol was obtained by supporting pre-synthesized Pt on TiO2. During the followed hydrogen reduction, strong hydrogen spillover occurred without the migration of TiO2 onto Pt. The reduced support then enhanced the electron transfer from TiO2 to Pt, increasing the percentage of partially negative Pt (Ptδ-), which has been confirmed by XPS. The strong EMSI made the obtained catalyst far more active than Pt/TiO2 prepared by impregnation method. The electron-enriched Pt/TiO2 achieved total organic carbon (TOC) conversion of 88.8% and TOF 149 h-1 at 100 °C and 2 MPa O2, while conventional Pt/TiO2 gave TOC conversion of 39.5% and TOF 41 h-1 for CWAO of phenol. Our work indicates that the enhancement of EMSI between metal and support can be an effective approach to develop highly active catalysts for phenol treatment.
Investigation of Antioxidant Mechanisms of Novel Peptides Derived from Asian Swamp Eel Hydrolysate in Chemical Systems and AAPH-Induced Human Erythrocytes

Wang X, Chen B, Bhullar KS, Yang H, Luo X, Fu J, et al.

Antioxidants (Basel), 2024 Jul 23;13(8).
PMID: 39199134 DOI: 10.3390/antiox13080888

Sixteen novel antioxidant peptides from Asian swamp eel (ASE) were identified in previous studies. However, their chemical and cellular antioxidant mechanisms remain unclear. Molecular docking of these peptides with ABTS and DPPH radicals revealed the critical role of hydrogen bonding and Pi-Pi stacking hydrophobic interactions between hydrophobic amino acid residues and free radicals. Residues, such as tryptophan, proline, leucine, and valine, played significant roles in these interactions. All these peptides exhibited notable erythrocyte morphoprotective effects in a model of AAPH-induced oxidative damage of human erythrocytes. Erythrocyte hemolysis was reduced primarily through the modulation of both non-enzymatic (GSH/GSSG) and enzymatic antioxidant systems (SOD, CAT, and GSH-Px) by these peptides. A decrease in levels of MDA, LDH release, and hemoglobin oxidation was observed. Among the peptides, VLYPW demonstrated superior chemical and cellular antioxidant activities, which may be attributed to its higher levels of tyrosine and tryptophan, as well as to its increased hydrophobic amino acid content.
Fulltext Hard-Carbon Negative Electrodes from Biomasses for Sodium-Ion Batteries

Lu B, Lin C, Xiong H, Zhang C, Fang L, Sun J, et al.

Molecules, 2023 May 11;28(10).
PMID: 37241775 DOI: 10.3390/molecules28104027

With the development of high-performance electrode materials, sodium-ion batteries have been extensively studied and could potentially be applied in various fields to replace the lithium-ion cells, owing to the low cost and natural abundance. As the key anode materials of sodium-ion batteries, hard carbons still face problems, such as poor cycling performance and low initial Coulombic efficiency. Owning to the low synthesis cost and the natural presence of heteroatoms of biomasses, biomasses have positive implications for synthesizing the hard carbons for sodium-ion batteries. This minireview mainly explains the research progress of biomasses used as the precursors to prepare the hard-carbon materials. The storage mechanism of hard carbons, comparisons of the structural properties of hard carbons prepared from different biomasses, and the influence of the preparation conditions on the electrochemical properties of hard carbons are introduced. In addition, the effect of doping atoms is also summarized to provide an in-depth understanding and guidance for the design of high-performance hard carbons for sodium-ion batteries.
News from a postpandemic world

Ellwanger JH, Lekgoathi MDS, Nemani K, Tarselli MA, Al Harraq A, Uzonyi A, et al.

Science, 2020 07 03;369(6499):26-29.
PMID: 32631879 DOI: 10.1126/science.abd1320

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