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  1. Akinbile CO, Yusoff MS
    Int J Phytoremediation, 2012 Mar;14(3):201-11.
    PMID: 22567705
    Water hyacinth (Eichhornia crassipes) and water lettuce (Pistia stratiotes) were analyzed to determine their effectiveness in aquaculture wastewater treatment in Malaysia. Wastewater from fish farm in Semanggol Perak, Malaysia was sampled and the parameters determined included, the pH, turbidity, dissolved oxygen (DO), chemical oxygen demand (COD), biochemical oxygen demand (BOD), nitrite phosphate (PO4(3-)), nitrate (NO(3-)), nitrite (NO(-2)), ammonia (NH3), and total kjedahl nitrogen (TKN). Also, hydroponics system was set up and was added with fresh plants weights of 150 +/- 20 grams Eichhornia crassipes and 50 +/- 10 grams Pistia stratiotes during the 30 days experiment. The phytoremediation treatment with Eichhornia crassipes had pH ranging from 5.52 to 5.59 and from 4.45 to 5.5 while Pistia stratiotes had its pH value from 5.76 to 6.49 and from 6.24 to 7.07. Considerable percentage reduction was observed in all the parameters treated with the phytoremediators. Percentage reduction of turbidity for Eichhornia crassipes were 85.26% and 87.05% while Pistia stratiotes were 92.70% and 93.69% respectively. Similar reductions were observed in COD, TKN, NO(3-), NH3, and PO4(3-). The capability of these plants in removing nutrients was established from the study. Removal of aquatic macrophytes from water bodies is recommended for efficient water purification.
  2. Akinbile CO, Yusoff MS
    Waste Manag Res, 2012 May;30(5):498-505.
    PMID: 21930519 DOI: 10.1177/0734242X11417974
    Solid waste comprised of a grass clippings mixture was decomposed using a locally-made compost bin in Pulau Pinang, Malaysia, to eliminate challenges associated with improper waste disposal. Bulk density, pH, moisture content, nutrients content, nitrogen (N), phosphorus (P), potassium (K), iron (Fe), zinc (Zn), copper (Cu) and carbon/nitrogen (C/N) ratio were determined over 77 days. A 34% reduction in compost bin volume was observed and bulk density and pH were also reduced from 732 to 482 kg m(-3) and 7.82 to 8.41, respectively, indicating fairly good performance. The final moisture content and C/N ratio were 44.06% and 14 : 1, respectively, and the results also showed that the presence of nutrients and heavy metals in the final compost were within acceptable limits for use as a soil conditioner. Final concentrations of N, P and K were 347 mg kg(-1), 510 mg kg(-1) and 14.8 g kg(-1) and for heavy metals, Fe, Zn and Cu were 5308, 300 and 20 mg kg(-1), respectively, which considerably assisted in the decomposition process. Processed waste materials from the bin were shown to be excellent organic fertilizers with over 75% germination index for seeds grown into bean sprouts in 72 h. An improved bin design to eliminate greenhouse gas emission into the environment is suggested.
  3. Akinbile CO, Yusoff MS, Ahmad Zuki AZ
    Waste Manag, 2012 Jul;32(7):1387-93.
    PMID: 22456086 DOI: 10.1016/j.wasman.2012.03.002
    Performance evaluation of pilot scale sub-surface constructed wetlands was carried out in treating leachate from Pulau Burung Sanitary Landfill (PBSL). The constructed wetland was planted with Cyperus haspan with sand and gravel used as substrate media. The experiment was operated for three weeks retention time and during the experimentation, the influent and effluent samples were tested for its pH, turbidity, color, total suspended solid (TSS), chemical oxygen demand (COD), biochemical oxygen demand (BOD(5)), ammonia nitrogen (NH(3)-N), Total phosphorus (TP), total nitrogen (TN) and also for heavy metals such as iron (Fe), magnesium (Mg), manganese (Mn) and zinc (Zn) concentrations. The results showed that the constructed wetlands with C. haspan were capable of removing 7.2-12.4% of pH, 39.3-86.6% of turbidity, 63.5-86.6% of color, 59.7-98.8% of TSS, 39.2-91.8% of COD, 60.8-78.7% of BOD(5), 29.8-53.8% of NH(3)-N, 59.8-99.7% of TP, 33.8-67.0% of TN, 34.9-59.0% of Fe, 29.0-75.0% of Mg, 51.2-70.5% of Mn, and 75.9-89.4% of Zn. The significance of removal was manifested in the quality of the effluent obtained at the end of the study. High removal efficiencies in the study proved that leachate could be treated effectively using subsurface constructed wetlands with C. haspan plant.
  4. Akinbile CO, Ogunrinde TA, Che Bt Man H, Aziz HA
    Int J Phytoremediation, 2016;18(1):54-61.
    PMID: 26121232 DOI: 10.1080/15226514.2015.1058330
    Two constructed wetlands, one with Azolla pinnata plant (CW1) and the other without (CW2) for treating domestic wastewaters were developed. Fifteen water parameters which include: Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD), Total Suspended Solid (TSS), Total Phosphorus (TP), Total Nitrogen (TN), Ammoniacal Nitrogen (NH3N), Turbidity, pH, Electrical Conductivity (EC), Iron (Fe), Magnesium (Mg), Manganese (Mn), and heavy metals such as Lead (Pb) and Zinc (Zn) were analyzed using standard laboratory procedures. The experiments were conducted in two (dry and wet) seasons simultaneously. Results showed considerable reductions in all parameters and metals including Zn in CW1 compared with CW2 in the two seasons considered while Pb and Mn were not detected throughout the study. Zn concentration levels reduced significantly in both seasons just as removal efficiencies of 70.03% and 64.51% were recorded for CW1 while 35.17% and 33.45% were recorded for CW2 in both seasons. There were no significant differences in the removal efficiencies of Fe in both seasons as 99.55%, 59.09%, 88.89%, and 53.56% were recorded in CW1 and CW2 respectively. Azolla pinnata has proved effective in domestic wastewater phytoremediation studies.
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