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Fulltext Effects of palm oil consumption on biomarkers of glucose metabolism: A systematic review

Zulkiply SH, Balasubramaniam V, Abu Bakar NA, Abd Rashed A, Ismail SR

PLoS One, 2019;14(8):e0220877.
PMID: 31415611 DOI: 10.1371/journal.pone.0220877

INTRODUCTION: Vegetable oil is an important source of fatty acids, and as palm oil being the highest consumed vegetable oil in many countries, its high saturated fatty acid content has led many concerns on cardiometabolic health. Dietary fatty acids has also been linked to affect glucose metabolism and insulin sensitivity. This systematic review is aimed at critically evaluating the available evidence on the association of palm oil with the biomarkers of glucose metabolism as compared to other vegetable oils.
METHODS: We systemically searched PubMed, CENTRAL and Scopus up to June 2018. We searched for published interventional studies on biomarkers of glucose metabolism (defined as fasting glucose, fasting insulin, HOMA, 2-hour post prandial glucose and HbA1C) that compared palm oil- or palm olein-rich diets with other edible vegetable oils (such as olive oil, canola oil and soybean oil). Two reviewers independently extracted data and assessed study risks of bias. Mean differences of outcomes were pooled for the meta-analysis.
RESULTS: We identified 1921 potentially eligible articles with only eight included studies. Seven randomised cross-over trials and one parallel trial were included. Study population were among young to middle-aged, healthy, non-diabetic, and normal weight participants. Intervention duration ranged from three to seven weeks, and fat substitution ranged from 15% to 20% energy. There were insignificant differences in fasting glucose when compared to partially hydrogenated soybean oil [-0.15mmol/L (-0.46,0.16) P = 0.33, I2 = 48%], soybean oil [0.05mmol/L (-0.09,0.18) P = 0.49, I2 = 0%] and olive oil [0.04mmol/L (-0.09,0.17) P = 0.76, I2 = 0%]. Insignificant effects were also seen on fasting insulin when compared to partially hydrogenated soybean oil [1.72pmol/L (-11.39,14.84) P = 0.80, I2 = 12%] and olive oil diet [-0.14pmol/L (-4.87,4.59) P = 0.95, I2 = 0%].
CONCLUSION: Current evidence on the effects of palm oil consumption on biomarkers of glucose metabolism is poor and limited to only healthy participants. We conclude that little or no additional benefit will be obtained by replacing palm oil with other oils rich in mono or polyunsaturated fatty acids for changes in glucose metabolism.

Matched MeSH terms: Vegetables
Fulltext Laser-Assisted High Speed Machining of 316 Stainless Steel: The Effect of Water-Soluble Sago Starch Based Cutting Fluid on Surface Roughness and Tool Wear

Yasmin F, Tamrin KF, Sheikh NA, Barroy P, Yassin A, Khan AA, et al.

Materials (Basel), 2021 Mar 09;14(5).
PMID: 33803364 DOI: 10.3390/ma14051311

Laser-assisted high speed milling is a subtractive machining method that employs a laser to thermally soften a difficult-to-cut material's surface in order to enhance machinability at a high material removal rate with improved surface finish and tool life. However, this machining with high speed leads to high friction between workpiece and tool, and can result in high temperatures, impairing the surface quality. Use of conventional cutting fluid may not effectively control the heat generation. Besides, vegetable-based cutting fluids are invariably a major source of food insecurity of edible oils which is traditionally used as a staple food in many countries. Thus, the primary objective of this study is to experimentally investigate the effects of water-soluble sago starch-based cutting fluid on surface roughness and tool's flank wear using response surface methodology (RSM) while machining of 316 stainless steel. In order to observe the comparison, the experiments with same machining parameters are conducted with conventional cutting fluid. The prepared water-soluble sago starch based cutting fluid showed excellent cooling and lubricating performance. Therefore, in comparison to the machining using conventional cutting fluid, a decrease of 48.23% in surface roughness and 38.41% in flank wear were noted using presented approach. Furthermore, using the extreme learning machine (ELM), the obtained data is modeled to predict surface roughness and flank wear and showed good agreement between observations and predictions.

Matched MeSH terms: Vegetables
Fulltext The impact of four processing methods on trypsin-, chymotrypsin- and alpha-amylase inhibitors present in underutilised legumes

Choi WC, Parr T, Lim YS

J Food Sci Technol, 2019 Jan;56(1):281-289.
PMID: 30728570 DOI: 10.1007/s13197-018-3488-0

The global trend in increasing plant-based protein diets due to health and ideological reasons, has created an increased demand for food legumes that exceeds current production. To meet this demand, it is timely to reduce relying solely on soybean, and explore the potential of the underutilised legumes that are cultivated regionally. Underutilised legumes are rich in protein, carbohydrates and other nutrients that are essential for consumer. However, relatively little is known about their anti-nutritional properties and processing methods. Anti-nutritional factors (ANFs) such as enzyme inhibitors are prevalent in legumes and may interfere with digestibility and nutrient absorption. Nevertheless, an optimised food processing method will overcome this challenge and warrant a safe inclusion of legume in plant-based protein diets. Hence current study aimed to optimise the food processing methods (soaking, wet heating, autoclaving and freezing) and evaluate their efficiency in eliminating the enzyme inhibitors [trypsin, chymotrypsin (CIA) and α-amylase (AIA) inhibitors] present in seven underutilised legumes. Current study showed that autoclaving at 121 °C for 15 min reduced the AIA in all underutilised legumes tested. The AIA and CIA of bambara groundnut were successfully inactivated by wet heating at 50 °C for 60 min, and by autoclaving at 121 °C for 15 min. While the CIA of chickpea was successfully inactivated by freezing at - 80 °C for 24 h.

Matched MeSH terms: Vegetables
First Report of Soft Rot Caused by Pectobacterium carotovorum subsp. carotovorum on Cucumber in Malaysia

Nazerian E, Sijam K, Zainal Abidin MA, Vadamalai G

Plant Dis, 2011 Nov;95(11):1474.
PMID: 30731752 DOI: 10.1094/PDIS-10-10-0754

Cucumber (Cucumis sativus L.) is one of the most important vegetable fruits in Malaysia. Cucumber is principally grown in the states of Johor, Kelantan, and Perak. The broad host range Enterobacteriaceae pathogen, Pectobacterium carotovorum, can cause soft rot on stems or cucumber fruit. In Malaysia, cucumber is produced in a warm, humid climate, thus the plant is susceptible to attack by P. carotovorum at any time during production. In 2010, cucumber samples with wilted and chlorotic leaves, water-soaked lesions, and collapsed fruits were found in multiple fields. Small pieces of infected stems and fruit were immersed in 5 ml of saline solution (0.85% NaCl) for 20 min and then 50 μl of this suspension was spread onto nutrient agar (NA) and incubated at 27°C for 24 h. White-to-pale gray colonies with irregular margins were selected for analysis. For pathogenicity tests, cucumber fruits were surface sterilized by ethyl alcohol 70%, washed with sterilized distilled water, cut into small pieces, and inoculated with 20 μl of 108 CFU/ml suspensions of five representative strains. Cucumber plants were grown for 3 weeks in sterilized soil and their stems were inoculated with 20 μl of 108 CFU/ml of bacterial suspension. Inoculated samples and control (noninoculated) plants were placed in a growth chamber with 80 to 90% relative humidity at 27°C. Symptoms occurred on fruit slices and stems after 1 to 3 days and appeared the same as naturally infected samples, but the control samples remained healthy. Koch's postulates were fulfilled with the reisolation of cultures with the same characteristics as described earlier. Hypersensitivity reaction (HR) assays were done by infiltrating 108 CFU/ml of bacterial suspension into tobacco leaf epidermis and HR developed. All strains were subjected to biochemical and morphological assays, as well as molecular assessment. The strains were gram negative, facultative anaerobes, rod shaped, able to macerate potato slices and growth at 37°C; catalase positive; oxidase and phosphatase negative; able to degrade pectate; sensitive to erythromycin; negative for utilization of α-methyl glycoside, indole production, and reduction of sugars from sucrose; acid production from arabitol, sorbitol, and utilization of citrate were negative, but positive for raffinose and melibiose utilization. PCR amplification of the pel gene by Y1 and Y2 primers produced a 434-bp fragment on agarose gel 1% (1). Amplification of intergenic transcribed spacer region by G1 and L1 primers gave two main bands at approximately 535 and 580 bp on agarose gel 1.5%. The ITS-PCR products were digested with RsaI restriction enzyme (3). On the basis of biochemical and morphological characteristics, PCR-based pel gene and characterization of the ITS region, and digestion of the ITS-PCR products with RsaI restriction enzyme, all isolates were identified as P. carotovorum subsp. carotovorum. To our knowledge, this is the first report of soft rot caused by P. carotovorum subsp. carotovorum on cucumber from Malaysia. References: (1) A. Darraas et al. Appl. Environ. Microbiol. 60:1437, 1994. (2) N. W Schaad et al. Laboratory Guide for the Identification of Plant Pathogenic Bacteria. 3rd ed. The American Phytopathological Society Press, St. Paul, 2001. (3) I. K. Toth et al. Appl. Environ. Microbiol. 67:4070, 2001.

Matched MeSH terms: Vegetables
First Report of Anthracnose Caused by Colletotrichum capsici on Bok Choy (Brassica chinensis) in Malaysia

Mahmodi F, Kadir JB, Wong MY, Nasehi A, Soleimani N, Puteh A

Plant Dis, 2013 May;97(5):687.
PMID: 30722185 DOI: 10.1094/PDIS-09-12-0843-PDN

Bok choy (Brassica chinensis L.) is a temperate vegetable grown in the cool highland areas of Malaysia. In June 2010, vegetable growing areas of the Cameron Highlands, located in Pahang State, Malaysia, were surveyed for the prevalence of anthracnose disease caused by Colletotrichum species. Diseased samples were randomly collected from 12 infested fields. Anthracnose incidence on bok choy varied from 8 to 36% in different nursery fields. Disease symptoms initially appeared as small water-soaked spots scattered on the leaf petioles of young plants. As these spots increased in size, they developed irregular round spots that turned to sunken grayish brown lesions surrounded by brownish borders. When the lesions were numerous, leaves collapsed. Pale buff to salmon conidial mass and acervuli were observed on well-developed lesions. The acervuli diameter varied in size from 198 to 486 μm, averaging 278.5 μm. Morphological and cultural characteristics of the fungus were examined on potato dextrose agar incubated for 7 days at 25 ± 2°C under constant fluorescent light. Vegetative mycelia were hyaline, septate, branched, and 2 to 7 μm in diameter. The color of the fungal colonies was grayish brown. Conidia were hyaline, aseptate, falcate, apices acute, and 21.8 to 28.5 × 2.6 to 3.4 mm. Setae were pale brown to dark brown, 75 to 155 μm long, base cylindrical, and tapering towards the acute tip. Appressoria were solitary or in dense groups, light to dark brown, entire edge to lobed, roundish to clavate, 6.5 to 14 × 5.8 to 8.6 μm, averaging 9.2 × 6.8 μm, and had a L/W ratio of 1.35. Based on the keys outlined by Mordue 1971 (2) and Sutton 1980 (3), the characteristics of this fungus corresponded to Colletotrichum capsici. Sequence analysis of the ITS-rDNA obtained from the Malaysian strain CCM3 (GenBank Accession No. JQ685746) using primers ITS5 and ITS4 (1) when aligned with deposited sequences from GenBank revealed 99 to 100% sequence identity with C. capsici strains (DQ286158, JQ685754, DQ286156, GQ936210, and GQ369594). A representative strain CCM3 was used for pathogenicity testing. Four non-infected detached leaves of 2-week-old B. chinensis were surface-sterilized and inoculated by placing 10 μl of conidial suspension (106 conidia ml-1) using either the wound/drop or non-wound/drop method, and distilled water was used as a control (1). Leaves were incubated at 25°C, 98% RH. The experiment was repeated twice. Five days after inoculation, typical anthracnose symptoms with acervuli formation appeared on the surface of tissues inoculated with the spore suspension, but not on the water controls. A fungus with the characteristics of C. capsici was recovered from the lesions on the inoculated leaves. Anthracnose caused by C. capsici has been reported on different vegetable crops, but not on bok choy (3). To the best of our knowledge, this is the first report of C. capsici causing anthracnose on bok choy in Malaysia. References: (1) R. Ford et al. Aust. Plant Pathol. 33:559, 2004. (2) J. E. M. Mordue. CMI Description of Pathogenic Fungi and Bacteria. Commonwealth Mycol. Inst., Kew, UK. 1971. (3) B. C. Sutton. The Genus Glomerella and its anamorph Colletotrichum. CAB International, Wallingford, UK, 1992. (4) P. P. Than et al. Plant Pathol. 57:562, 2008.

Matched MeSH terms: Vegetables
Fulltext Blood cholinesterase activity and associated factors among pesticide-exposed vegetable family farmers in Sabah Malaysia

Bitinsun Botinggo, Khamisah Awang Lukman, Mohammad Saffree Jeffree, Saihpudin @ Sahipudin Saupin

Malaysian Journal of Medicine and Health Sciences, 2019;15(106):9-9.
MyJurnal

Introduction: Chemical pesticide is commonly used among vegetable family farmers in Sabah. There is no system to monitor adverse health effects. This study is to determine the blood cholinesterase activity and associated factors among vegetable family farmers. Methods: A cross sectional study has involved 163 farmers aged 18-60 years old. Validated questionnaire was used to assess the associated factors. Lovibond AF267 Cholinesterase Kit was used to determine the blood cholinesterase activity. Chi square test was used to determine the association between blood cholinesterase activity and socio demographic factors and work practices. Results: Approximately 23.3% of study sample had low blood cholinesterase activity. There were significant associations between low blood cholinesterase activity with older age group (p

Matched MeSH terms: Vegetables
Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits

Voon WW, Rukayadi Y, Meor Hussin AS

Lett Appl Microbiol, 2016 May;62(5):428-33.
PMID: 27002476 DOI: 10.1111/lam.12568

Biocellulose (BC) is pure extracellular cellulose produced by several species of micro-organisms that has numerous applications in the food, biomedical and paper industries. However, the existing biocellulose-producing bacterial strain with high yield was limited. The aim of this study was to isolate and identify the potential biocellulose-producing bacterial isolates from Malaysian acidic fruits. One hundred and ninety-three bacterial isolates were obtained from 19 local acidic fruits collected in Malaysia and screened for their ability to produce BC. A total of 15 potential bacterial isolates were then cultured in standard Hestrin-Schramm (HS) medium statically at 30°C for 2 weeks to determine the BC production. The most potent bacterial isolates were identified using 16S rRNA gene sequence analysis, morphological and biochemical characteristics. Three new and potent biocellulose-producing bacterial strains were isolated from soursop fruit and identified as Stenotrophomonas maltophilia WAUPM42, Pantoea vagans WAUPM45 and Beijerinckia fluminensis WAUPM53. Stenotrophomonas maltophilia WAUPM42 was the most potent biocellulose-producing bacterial strain that produced the highest amount of BC 0·58 g l(-1) in standard HS medium. Whereas, the isolates P. vagans WAUPM45 and B. fluminensis WAUPM53 showed 0·50 and 0·52 g l(-1) of BC production, respectively.

Matched MeSH terms: Vegetables
Fulltext EFFECT OF PHOTO-SELECTIVE NETTING ON GROWTH DEVELOPMENT OF GREEN AMARANTH (Amaranthus viridis L.)

SITI NABILAH MOHAMMAD SABRI, NURUL FAZIHA IBRAHIM, SITI NABILAH MOHAMMAD SABRI, SUHAIZAN LOB

UMT Journal of Undergraduate Research, 2020;2(3):67-72.
MyJurnal

Green amaranth (Amaranthus viridis L.) is one of the most popular leafy vegetables cultivated in Malaysia. Photo-selective netting is a revolutionary agricultural-technical idea, allowing traditional nets to be improved to a sophisticated level, which can impact microclimate and solar radiation as well as protect crops from pests. This study aims to investigate the growth performance of green amaranth under different three different treatments of photo-selective nets including black, and yellow shade nets and control. The assessed physiological, pre-harvest and post-harvest parameters were air temperature and relative humidity, measurement of absorption of photosynthetically active radiation (PAR), plant height, number of leaves, and dry weight. This study found that the yellow shade net showed the best performance in plant growth with the average difference in plant height (4.11 cm), average leaves number (7 leaves) and average dry weight (18.58 mg) were the highest compared to black shade net and control (without shading). In conclusion, photo-selective yellow shade net was able to enhance the growth development of green amaranth and could be applied in the large scale production to boost the growth and yield of leafy vegetables.

Matched MeSH terms: Vegetables
Fulltext Antioxidant properties of total lutein content in different parts of pumpkin (Cucurbita maxima)

WONG YEN WEN, FAUZIAH TUFAIL AHMAD

UMT Journal of Undergraduate Research, 2020;2(3):27-34.
MyJurnal

Pumpkin (Cucurbita maxima) is a vegetable crop which is commonly consumed as vegetables or incorporated into food products. Pumpkin flesh was reported abundant with carotenoid compounds includes α-carotene, β-carotene, β-cryptoxanthin, lutein and zeaxanthin. As this antioxidant related to the colour pigment, these nutrients highly potential to be in other parts of pumpkin such as peel and seed. Therefore, the aim of this was to determine the total lutein content in different parts of pumpkin and their antioxidant properties. The pumpkin would be collected and evaluated at the commercial maturity stage (60% to fully orange-yellow of fruit peel). The presence of the lutein properties using DPPH, FRAP and ABTS assays in different parts of pumpkin was tested by using microplate spectrophotometer and analysed statistically with SPSS version 20. Among of all pumpkin fruit parts, flesh presented the highest concentration of potential lutein extracts, followed by peel and seed, respectively. On the contrary, potential lutein extracts from the pumpkin peel had the highest antioxidant activity in terms of DPPH, FRAP and ABTS assays when compared to the lutein extracts in flesh and seeds. This study indicates that the potential lutein from Cucurbita maxima especially peel may be the alternative to be used as both natural antioxidants in food products due to increasing demand for natural food preservatives. Therefore, this study may act as a source for others to further study to optimize the usage of pumpkin by-products

Matched MeSH terms: Vegetables
Fulltext Occurrence of Aflatoxins in Edible Vegetable Seeds and Oil Samples Available in Pakistani Retail Markets and Estimation of Dietary Intake in Consumers

Waqas M, Iqbal SZ, Abdull Razis AF, Pervaiz W, Ahmad T, Usman S, et al.

Int J Environ Res Public Health, 2021 07 29;18(15).
PMID: 34360308 DOI: 10.3390/ijerph18158015

Aflatoxins (AFs) are secondary metabolites toxic to humans as well as animals. The environmental conditions, conventional agricultural practices, and illiteracy are the main factors which favor the production of AFs in food and feed. In the current study 744 samples of vegetable seeds and oils (soybean, sunflower, canola, olive, corn, and mustard) were collected and tested for the presence of aflatoxin B1 (AFB1) and total AFs. Liquid-liquid extraction was employed for the extraction of AFs from seeds and oil samples. Reverse phase high performance liquid chromatography equipped with fluorescence detection was used for the analysis. The results have shown that 92 (56.7%) samples of imported and 108 (57.0%) samples of local edible seeds were observed to be contaminated with AFs. All samples of edible seeds have AFB1 levels greater than the proposed limit set by the European Union (EU, 2 µg/kg) and 12 (7.40%) samples of imported seeds and 14 (7.40%) samples of local seeds were found in the range ≥ 50 µg/kg. About 78 (43.3%) samples of imported edible oil and 103 (48.3%) sample of local edible oil were observed to be positive for AFs. Furthermore, 16 (8.88%) and six (3.33%) samples of imported vegetable oil have levels of total AFs in a range (21-50 µg/kg) and greater than 50 µg/kg, respectively. The findings indicate significant differences in AFs levels between imported and local vegetable oil samples (t = 22.27 and p = 0.009) at α = 0.05 and a significant difference in AFs levels were found between vegetable seeds and oil samples (t = -17.75, p = 0.009) at α = 0.05. The highest dietary intake was found for a local sunflower oil sample (0.90 µg/kg/day) in female individuals (16-22 age group). The results have shown considerably high levels of AFB1 and total AFs in seeds and oil samples and emphasise the need to monitor carefully the levels of these toxic substances in food and feed on regular basis.

Matched MeSH terms: Vegetables
Fulltext Ph Monitoring for Liquid Fertilizer Management in Black Pepper Farming

Kedung Fletcher, Anding Nyuak, Tan Phei Yee

International Journal of Innovation and Industrial Revolution, 2021;3(6):1-12.
MyJurnal

There is lacking technology application in black pepper farming to automate daily routine activities in monitoring black pepper vines growth and nutrient need. With the revolution of Industry 4.0 (IR4.0), and tremendous improvement in the internet of things (IoT), the application of precision agriculture to pepper farming is a thing to consider for its benefit. This paper to explore the use of IoT to monitor fertilizer requirement for pepper vines using pH sensor. The pH sensor attached to Raspberry Pi 3 will be collecting the data and forwarding it to the cloud database for farmer reference and take decision based on data presented in form of a digital report from the database. The Python environment provides the space for coding in Raspberry Pi. SQL and PHP software is used to design the user interface and data management in the relational database management system. The information about pH provides a better understanding of how pH parameter affects the growth of pepper vines. The farmer will be able to access the information anywhere and anytime. Therefore, our proposed system will greatly help the pepper farmers in Sarawak in managing the usage of fertilizer as a way to minimize farm inputs, thus increase their profit.

Matched MeSH terms: Vegetables
Fulltext Association between home environment, dietary practice, and physical activity among primary school children in Selangor, Malaysia

Woon, F.C., Chin, Y.S., Kaartina, S., Fara Wahida, R., Hiew, C.C., Mohd Nasir, M.T.

Malays J Nutr, 2014;20(1):1-14.
MyJurnal

Introduction: The home environment plays an influential role in affecting dietary and physical activity practices of children. This study aimed to determine the association between the home environment, dietary practice and physical activity among primary school children in Selangor. Methods: This cross-sectional study was conducted in five primary schools selected using multistage stratified sampling. A total of 293 children (32.8% males and 67.2% females) (mean age of 11.0±0.9 years) and their parents (10.7% fathers and 89.3% mothers) completed the study. Dietary practice of the children was assessed using a two-day dietary recall. Energy expenditure and physical activity of the children were assessed using a two-day physical activity recall. Parents of the children completed the Home Environment Survey (HES). Results: The mean energy intake of the children was 1765±416 kcal/day with 75.0% not achieving the Malaysian Recommended Nutrient Intake (RNI) for energy. Almost all children (96.5%) were physically inactive, with a mean energy expenditure of 1269±342 kcal/day. High availability of fruit/vegetable at home was associated with high vegetable intake (r=0.128, p

Matched MeSH terms: Vegetables
Fulltext Occurrence of Intestinal Parasitic Contamination in Select Consumed Local Raw Vegetables and Fruits in Kuantan, Pahang

Yusof AM, Mohammad M, Abdullahi MA, Mohamed Z, Zakaria R, Wahab RA

Trop Life Sci Res, 2017 Jan;28(1):23-32.
PMID: 28228914 MyJurnal DOI: 10.21315/tlsr2017.28.1.2

Intestinal parasitic infections are one of the most common causes of human diseases that result in serious health and economic issues in many developing and developed countries. Raw vegetables and fruits play an important role in transmitting parasites to humans. Hence, the aim of this study was to investigate the parasitological contamination of select commonly consumed local leafy vegetables and fruits in Kuantan, Malaysia. One kilogram of locally consumed raw vegetables and fruits were collected randomly from the Kuantan wet market (Pasar Tani) during the monsoon season (November 2014-January 2015) and the dry season (February 2015-April 2015). A standard wet mount procedure and modified Ziehl-Neelsen staining were used for the detection of parasites. In the present study, the examination of vegetables revealed five different parasite species. The vegetable samples collected from Kuantan's wet market were positive for both helminthes and protozoa. However, the fruits samples were negative for parasitic contamination. Pegaga was the most contaminated leafy vegetable in this study, and Strongyloides was the parasite found most frequently. Furthermore, there was a high diversity in the type of parasites observed during the dry season compared to the monsoon season. Therefore, further action should be taken to reduce the occurrence of parasitic contamination in vegetables by implementing the principles of good agricultural practice and improving water treatment efficacy.

Matched MeSH terms: Vegetables
Fulltext Metabolic engineering of functional phytochemicals

Kabir, M.U., Abdulkarim, S.M., Son, R., Azizah, A.H., Saari, N.B.

International Food Research Journal, 2013;20(1):35-41.
MyJurnal

Phytochemicals belonging to the group’s phenols, terpenes, betalains, organosulfides, indoles and protein inhibitors are important components in fruits, vegetables, legumes, whole grains and nuts that have health promoting benefits and a variety of applications in food and pharmaceutical industries. Initially only a few of these important phytochemicals are produced commercially by chemical synthesis. However, recent developments in the field of biotechnology have provided metabolic engineering strategies that use microorganisms as cell factories for high production of these products. This review will discuss the general biosynthetic pathways, metabolic engineering and optimization strategies of functional phytochemicals that have received a lot of attention from investigators.

Matched MeSH terms: Vegetables
Fulltext Occurrence of Norovirus GI in green and red onion

Noor Hidayah, M.S., Tuan Zainazor, C., Pui, C.F., Noorlis, A., Noor Eliza, M.R., Naziehah, M.D., et al.

International Food Research Journal, 2011;18(2):677-681.
MyJurnal

Several Norovirus cases due to consumption of green onions have been reported during recent years but reports on red onions are not found. Onions are one of the major tastes in Malaysian food which are sometimes consuming raw especially the green onion. Viral contamination in onions can occur due to planting condition and not properly prepared food. This situation can pose the human health risk. A method was developed to detect the Norovirus that might present on different type of onions. In this study, 60 samples were collected from local market. Elution by Tryptose Phosphate Glycine broth and concentration steps using negatively charge filter were applied to enhance the detection of virus in food due to low copies of virus on food surface. The viral RNA was extracted using Qiagen Rneasy Mini kit before further detection using One-step RT-PCR. The total incidence of Norovirus in green onion and red onion was 13.33% (4/30) and 3.33 % (1/30) respectively. This is the first report of the detection of Norovirus in red and green onions in Malaysia. Based on the results, it is concluded that this method is reliable to detect Norovirus on onions and vegetables surface and hence can be applied in the laboratories for routine or food borne outbreak investigation.

Matched MeSH terms: Vegetables
Fulltext Nutritional profile and antioxidative properties of selected tropical wild vegetables

Ng, X.N., Chye, F.Y., Mohd Ismail, A.

International Food Research Journal, 2012;19(4):1487-1496.
MyJurnal

Five underutilized wild vegetables namely Limnophila aromaticoides, Ceratopetris thalictroides, Crassocephalum crepidioides, Etlingera elatoir and Monochoria vaginalis were analyzed for nutritional values, phenolic components and antioxidant activities. These wild greens were found to have high fibre (11.3-19.8 g/100g) and ash (13.0-17.6 g/100g) contents as compared to commercialized species, Brassica juncea. The iron content of Monochoria vaginalis is four times higher than Brassica juncea (33.1 mg/g dry weight). Crassocephalum crepidioides demonstrated remarkable lipid peroxidation inhibition (90.4%). The phenolic content of Etlingera elatoir is two times higher than Brassica juncea. Thus, it is of both great free radical scavenger and iron chelators with the lowest EC50 values of 1.8 mg/ml and 2.3 mg/ml respectively. As a conclusion, these wild vegetables could be potentially used in alleviating micronutrients deficiency especially for the rural populace and as a potent source of natural antioxidants.

Matched MeSH terms: Vegetables
Updates on Managing Type 2 Diabetes Mellitus with Natural Products: Towards Antidiabetic Drug Development

Alam F, Islam MA, Kamal MA, Gan SH

Curr Med Chem, 2018;25(39):5395-5431.
PMID: 27528060 DOI: 10.2174/0929867323666160813222436

Over the years, natural products have shown success as antidiabetics in in vitro, in vivo studies and clinical trials. Because natural product-derived drugs are more affordable and effective with fewer side-effects compared to conventional therapies, pharmaceutical research is increasingly leaning towards the discovery of new antidiabetic drugs from natural products targeting pathways or components associated with type 2 diabetes mellitus (T2DM) pathophysiology. However, the drug discovery process is very lengthy and costly with significant challenges. Therefore, various techniques are currently being developed for the preclinical research phase of drug discovery with the aim of drug development with less time and efforts from natural products. In this review, we have provided an update on natural products including fruits, vegetables, spices, nuts, beverages and mushrooms with potential antidiabetic activities from in vivo, in vitro and clinical studies. Synergistic interactions between natural products and antidiabetic drugs, and potential antidiabetic active compounds from natural products are also documented to pave the way for combination treatment and new drug discovery, respectively. Additionally, a brief idea of the drug discovery process along with the challenges that arise during drug development from natural products and the methods to conquer those challenges are discussed to create a more convenient future drug discovery process.

Matched MeSH terms: Vegetables
Fulltext Biosafety of Vibrio parahaemolyticus from vegetables based on antimicrobial sensitivity and RAPD profiling

Tunung, R., Jeyaletchumi, P., Noorlis, A., Tang, Y.H., Sandra, A., Ghazali, F.M., et al.

International Food Research Journal, 2012;19(2):467-474.
MyJurnal

This study was undertaken to characterize the antibiotic resistance and randomly amplified polymorphic DNA (RAPD) profiles of Vibrio parahaemolyticus isolates from raw vegetable samples. A total of 46 isolates of V. parahaemolyticus recovered from raw vegetables samples and were confirmed by PCR were analyzed in this study. Most of the isolates were resistant to nalidixic acid (93.48%) and were the least resistant towards imipinem (4.35%). The MAR index results also demonstrated high individual and multiple resistances to antibiotics among the isolates. From the RAPD analysis, the size for RAPD fragments generated ranged from 250 bp to 1,500 bp, with most of the strains contained three major gene fragments of 350, 1,000 and 1,350 bp. The RAPD profiles revealed a high level of DNA sequence diversity within the isolates. Antibiotic resistance and RAPD proved to be effective tools in characterizing and differentiating the V. parahaemolyticus strains.

Matched MeSH terms: Vegetables
Fulltext Anticancer effect of underutilized fruits

Rabeta, M.S., Chan, S., Neda, G.D., Lam, K.L., Ong, M.T.

International Food Research Journal, 2013;20(2):551-556.
MyJurnal

Plants, particularly fruits and vegetables, have many phytochemicals that possess various bioactivities, including antioxidant and anticancer properties. In this study, the aim was to investigate the antiproliferative properties of Syzygium fruits, namely water apple (Syzygium aqueum), milk apple (Syzygium malaccense), and malay apple (Syzygium malaccense L.) against two types of cancer-origin cells, namely MCF-7 (hormone dependent breast cancer cell line) and MDA-MB-231 (nonhormone-dependent breast cancer cell line). Two solvent methods were prepared using aqueous and methanol extraction. Antiproliferation activities of these extracts were evaluated by employing colorimetric MTT (3-(4,5-dimethylthiazol-2-yl)2,5 diphenyltetrazolium bromide) assay through time periods of 24, 48, and 72 hours. The result showed that extracts from the three fruits had no significant effects for 24 and 48 hours time periods (p >0.05) but extracts of Water apple and Malay apple displayed antiproliferation effects on MCF-7 cell lines (p

Matched MeSH terms: Vegetables
Fulltext Carotenoids retention in leafy vegetables based on cooking methods

Chang, S.K., Nagendra Prasad, K., Amin, I.

International Food Research Journal, 2013;20(1):457-465.
MyJurnal

Green leafy vegetables are good sources of carotenoids. Generally, food composition databases related to raw foods are available, but data on cooked foods in Malaysia are still lacking. Since carotenoids are prone to degradation during cooking processes, the present study was undertaken to evaluate the extent of nutrient loss in vegetables subjected to two conventional cooking methods, which were boiling and stir-frying with cooking durations of 4 and 8 minutes. The vegetables selected were Chinese cabbage (Brassica Pekinensis var. cephalata), swamp cabbage (Ipomoea aquatica), spinach (Spinacia oleracea), Ceylon spinach (Basella rubra), red spinach (Amaranthus gangeticus), white spinach (Amaranthus viridis) and tapioca shoots (Manihot utilissima). Percentage losses of nutrients after cooking treatment were calculated based on retention factors. Results obtained showed that stir-frying had reduced lutein content for all vegetables ranging from 8-89% while the effect of boiling for lutein varied (0-428%) with different vegetables at both cooking durations of 4 and 8 min. Boiling for 8 min increased retention of β carotene in all vegetables ranging from 18-380% except for Chinese cabbage and spinach compared with 4 min, while stir-frying generally increased the retention of β-carotene for all vegetables 2-3 times except for spinach. Cooked vegetables have variations in carotenoids composition brought by varying cooking conditions (time and temperature), type of vegetables and the interaction between cooking methods and type of vegetables.

Matched MeSH terms: Vegetables

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